Medium, Per Se, For Culture, Maintenance, Regeneration, Etc. Patents (Class 435/431)
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Patent number: 7683244Abstract: An object of the present invention is to develop a novel Lobelia plant and a breeding method thereof, which allow for first-filial-generation (F1) hybrids exhibiting morphological forms (having the advantages of their two parents and far exceeding the traits of the two parents) desired by their breeder. The present invention thus provides a novel Lobelia plant by crossing Lobelia richardsonii (seed parent) and Lobelia valida (pollen parent), which while being a hybrid in first-filial-generation (F1) population of the genetically stable wild species, nevertheless the F1 population exhibits an even distribution over a range from a hybrid close to the seed parent to a hybrid close to the pollen parent.Type: GrantFiled: March 27, 2006Date of Patent: March 23, 2010Assignee: Kirin Agribio Company, LimitedInventor: Daigaku Takeshita
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Publication number: 20100035327Abstract: The invention is used as an additive to a culture medium to maintain and grow various cells including stem cells and support their manufactured and secreted products including cytokines, chemokines and growth factors in an environment which provides: a) metabolic enabling or enhancement of measurable parameters within the cellular population b) availability and usage of nutrients to cells c) distinct and positive effect on cellular dynamics (i.e. cellular proliferation and secretion of manufactured products by the cells) d) stabilized environment for maintaining conditions for growth and other cellular and metabolic processes including secretion of manufactured products including signaling factors e) enhanced biological cellular function of inherent cellular processes f) non-interference with normal cellular metabolics (i.e. signaling) g) increased protection from toxic effects to the cell h) additional benefits to the cellular population which in absence of the additive would be lacking.Type: ApplicationFiled: August 11, 2008Publication date: February 11, 2010Inventors: Ann Marie Steele, Mark Dargan Smith, Somchai Boonchuen
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Publication number: 20100024081Abstract: Described herein are methods and media for facilitating somatic embryogenesis and for collecting, conditioning, and transferring the washed embryos onto a substrate and into an environment suitable for conditioning the embryos for a desired period of time so they become germination-competent for plant production. The described plant embryo cleaning apparatus and method are used for preparing multiple plant embryos for plant production. The apparatus and method can use a cleaning fluid source, a fluid-conditioning system, a fluid-delivery structure, a cleaning station, an outlet mechanism, a negative pressure source, and a controller.Type: ApplicationFiled: July 29, 2009Publication date: January 28, 2010Inventors: John Joseph Clark, Narender Singh Nehra, Mark Russell Rutter, Jessica S. Sage, Sydney Keith Seymour, Timothy Joel Stout, George Surritte, Ronald W. Winkles
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Publication number: 20100000152Abstract: The present invention aims at providing a method for producing a nutrient solution for plant culture utilizing an organic material and providing a method of hydroponic culture in which a plant can be cultured while directly adding the organic material to the nutrient solution. The present invention provides a method for producing a biomineral-containing substance comprising establishing a microbial ecosystem necessary for stable mineralization of organic material by adding the organic material to water gradually or at one time and fermenting the resulting mixture and a method of hydroponic culture comprising utilizing the biomineral-containing substance obtained according to the method as at least a part of the nutrient solution and culturing the plant while directly adding the organic material to the nutrient solution.Type: ApplicationFiled: December 4, 2006Publication date: January 7, 2010Inventors: Makoto Shinohara, Yoichi Uehara, Masato Kouno, Hirofumi Iwakiri
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Patent number: 7629502Abstract: The present invention provides for a DNA construct comprising a promoter, a TSB1 gene, and a genetic modification, wherein the TSB1 gene and the genetic modification are promoted by the same said promoter. Additionally, the present invention provides for a plant that is transfected by this DNA construct, and a method of selecting this transfected plant.Type: GrantFiled: December 7, 2006Date of Patent: December 8, 2009Assignee: Academia SinicaInventor: Ming-Tsair Chan
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Publication number: 20090275105Abstract: Provided are methods of producing, via induction, plant products such as bromelain from plants of the genus Ananas established in vitro. A process for extracting bromelain from tissues of the pineapple (Ananas comosus) plant that have been established and induced in vitro is provided. The plants have a higher proteolytic activity (200 to 600%)—and in high concentrations—as compared with the protein generated naturally in plants and as currently extracted using conventional methodologies.Type: ApplicationFiled: June 21, 2006Publication date: November 5, 2009Inventor: Barba Ortiz
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Patent number: 7572632Abstract: Methods and compositions involving nutritive media, media supplements, media subgroup and buffer formulations are provided. Powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent are also disclosed. Methods of producing these media, media supplements, media subgroups and buffer formulations as well as kits and methods for cultivation of procaryotic and eukaryotic cells (including human cells) are also provided. Also disclosed are methods of producing sterile powdered media, media supplements, powdered growth factors, media subgroup and buffer formulations. Methods of sterilization include gamma irradiation. Methods for producing dry cell powders where spray-drying a cell suspension may be used are disclosed. Cells, media, media supplements, media subgroups and buffer powders produced by these methods are also disclosed.Type: GrantFiled: October 16, 2003Date of Patent: August 11, 2009Assignee: Life Technologies CorporationInventors: Richard Fike, William Whitford, William Biddle
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Patent number: 7569746Abstract: The invention relates to the use of a modified Agrobacterium to deliver proteins directly to plant cells. Proteins of interest are delivered to the plant host in the form of a fusion protein with the Agrobacterium virulence protein VirF. Nucleotide sequences encoding such fusion proteins of VirF and a protein of interest are provided. Also provided are bacteria modified to comprise such fusion proteins of VirF and a protein of interest. Methods of introducing such fusion proteins into a plant host are provided. The invention finds use in facilitating plant transformation and particularly in the bio-engineering of desirable traits into crop plants.Type: GrantFiled: August 6, 2004Date of Patent: August 4, 2009Assignees: Pioneer Hi-Bred International, Inc., Purdue Research FoundationInventors: Matthew A. Bailey, Stanton B. Gelvin, Saikat Bhattacharjee, William J. Gordon-Kamm, Keith S. Lowe, Yumin Tao
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Patent number: 7521238Abstract: The present invention provides a method and a culture medium for the production of food biomass by directly culturing seed kernel tissue, or seed cotyledonary differentiated tissue. The culture medium of the present invention contains at least DKW culture medium, Vitamin MS culture medium with an enriched concentration of thiamine, sacarose, kinetine, adenine, 2,4diclorofenoxiacético (2,4D), L-Glutamine, cysteine, ascorbic acid, and Gelrite®.Type: GrantFiled: December 20, 2006Date of Patent: April 21, 2009Assignee: Universidad De AntioquiaInventors: Lucia Atehortua, Esther Julia Naranjo, Andrea Lorena Herrera, Adriana Maria Gallego
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Publication number: 20090087889Abstract: Bottom dwelling colonies of Botryococcus are stimulated to produce and accumulate unusually high hydrocarbon concentrations that make them float to the surface. When exposed to carbon dioxide gas at the surface of the water, hydrocarbon induced flotation and growth is further enhanced with concomitant provision of appropriate light and available nitrogen. Botryococcus var. Ninsei is distinct from previously cultured strains of the genus in color, metabolism, and niche. The Ninsei variety grows green at the air-water interface. Metabolically, Ninsei is distinct from other varieties because it produces hydrocarbons in the presence of surplus ammonia in contrast to other varieties that shut down hydrocarbon production when ammonia is available. These characteristics are consistent with growth on the surface at water's edge, e.g., in the niche characterized by a mud alga. The surface niche for Ninsei is distinct because all other varieties are submerged, occupying the floor, e.g.Type: ApplicationFiled: May 5, 2006Publication date: April 2, 2009Inventor: Arthur M. Nonomura
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Publication number: 20090083883Abstract: The present invention relates to improved methods and means for transformation of soybean (Glycine max) based on a D-alanine and/or D-serine selection.Type: ApplicationFiled: March 16, 2007Publication date: March 26, 2009Applicant: BASF Plant Science GmbHInventors: Diana Arias, Sara Price, Leslie Grist, Ming Cheng, Haiping Hong, Libby Bernal, Paula Olhoft, Hee-Sook Song, Luke Mankin
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Publication number: 20090055968Abstract: The present invention relates to a method of reprogramming plant development that allows flower buds and seeds to arise de novo, directly from a cotyledon or radicle explants or from shoots produced on a cotyledon or radicle. The present invention also provides for an improved culturing media that provide for in vitro flowering.Type: ApplicationFiled: October 17, 2008Publication date: February 26, 2009Applicant: University Of Toledo, A University Instrumentally Of The State Of OhioInventors: Sairam V. Rudrabhatla, Stephen L. Goldman
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Publication number: 20080299535Abstract: [Problem] To provide a preservative solution for cells, tissues and organs using a rare sugar and a preservation method using the solution. [Means for Resolution] A preservative solution for cryopreservation of animal or human organs and animal or plant tissues or cells, the solution containing a rare sugar D-allose. A preservative solution for cryopreservation of the human kidney, cells derived from animals or humans, or human or animal sperm and/or ovum and/or fermented ovum in which the cryopreservation is conducted at from ?5 to 20° C. A preservative solution for cryopreservation of cells derived from animals or humans, or human or animal sperm and/or ovum and/or fermented ovum in which the cryopreservation is conducted at a temperature at which to start freezing to ?196° C.Type: ApplicationFiled: May 26, 2005Publication date: December 4, 2008Applicant: NATIONAL UNIVERSITY CORPORATION KAGAWA UNIVERSITYInventors: Masaaki Tokuda, Masaaki Ueki, Rikiya Taoka, Yoshiyuki Kakehi, Ken Izumori
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Publication number: 20080274547Abstract: Use of nitric oxide modulators during the transformation process can enhance Agrobacterium-mediated transformation of plants.Type: ApplicationFiled: May 30, 2008Publication date: November 6, 2008Inventors: David R. Duncan, Cristina Ubach
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Patent number: 7445932Abstract: Solanum viarum is an alkaloid producing plant of the family Solanaceae with varied therapeutic uses. In order to grow the plant in large areas; one needs to have an efficient system of vegetative multiplication, which ensures its genetic uniformity, and true to the type nature. In nature largely seeds propagate plant, which could be result of cross-pollination which may result in genetic drift. The present invention provides an efficient micropropagation system, with high level of multiplication at relatively low cost of production. The multiplication ratio was as high as 1:6 and almost 95% the plants were viable and successfully cultivated in field. The present invention provides an ideal way of mass cultivation of the selected elite plant material.Type: GrantFiled: June 3, 2003Date of Patent: November 4, 2008Assignee: Reliance Life Sciences Pvt. Ltd.Inventors: Anil Kush Kumar, Debasis Patnaik
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Patent number: 7402433Abstract: The present invention relates to an efficient method of in-vitro micropropagation of Piper longum plants. In particular, the present invention is directed towards the novel method for micropropagation of Piper longum from lateral bud (meristematic) explant (starting material), by culturing the explants on different medias. This method results in mass production of the plant Piper longum in a short span of time.Type: GrantFiled: April 10, 2003Date of Patent: July 22, 2008Assignee: Reliance Life Sciences Pvt. Ltd.Inventors: Madhuri Sharon, Ghanshyam Maurya
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Publication number: 20080172753Abstract: The invention provides seed and plants of the bean line designated EX 15340804. The invention thus relates to the plants, seeds and tissue cultures of bean line EX 15340804, and to methods for producing a bean plant produced by crossing a plant of bean line EX 15340804 with itself or with another bean plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of bean line EX 15340804, including the pods and gametes of such plants.Type: ApplicationFiled: January 12, 2007Publication date: July 17, 2008Inventors: George Kotch, Ken Kmiecik
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Publication number: 20080153165Abstract: The present invention provideS a method and a culture medium for the production of food biomass by directly culturing seed kernel tissue, or seed cotyledonary differentiated tissue. The culture medium of the present invention contains at least DKW culture medium, Vitamin MS culture medium with an enriched concentration of thiamine, sacarose, kinetine, adenine, 2,4diclorofenoxiacetico (2,4D), L-Glutamine, cysteine, ascorbic acid, and Gelrite®.Type: ApplicationFiled: December 20, 2006Publication date: June 26, 2008Inventors: Lucia Atehortua, Esther Julia Naranjo, Andrea Lorena Herrera, Adriana Maria Gallego
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Patent number: 7368631Abstract: The present invention is directed to a method of Acacia mangium regeneration through organogenesis and a method of genetic transformation of Acacia mangium. The method of regeneration comprises inducing callus from different parts of seedlings and vegetatively micropropagated plantlets as explants; adventitious bud induction followed by pinnate leaf and bud elongation and eventually, elongated shoots were induced to root. Based on the regeneration system, a marker gene GUS under cauliflower mosaic virus promoter was introduced to Acacia mangium via Agrobacterium infection. GUS staining in the regenerated plants and Southern blot hybridization prove the incorporation of the foreign gene into the host genome and expression of the foreign gene.Type: GrantFiled: December 9, 2004Date of Patent: May 6, 2008Assignee: Temasek Life Sciences Laboratory LimitedInventors: Deyu Xie, Yan Hong
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Patent number: 7264951Abstract: This invention provides methods whereby taxol, baccatin III, and other taxol-like compounds, or taxanes, can be produced in very high yield from all known Taxus species, e.g., brevifolia, canadensis, cuspidata, baccata, globosa, floridana, wallichiana, media and chinensis. Particular modifications of culture conditions (i.e., media composition and operating modes) have been discovered to enhance the yield of various taxanes from cell culture of all species of Taxus. Particularly preferred enhancement agents include silver ion or complex, jasmonic acid (especially the methyl ester), auxin-related growth regulators, and inhibitors of the phenylpropanoid pathway, such as 3,4-methylenedioxy-6-nitrocinnamic acid. These enhancement agents may be used alone or in combination with one another or other yield-enhancing conditions. While the yield of taxanes from plant cell culture of T.Type: GrantFiled: May 22, 1998Date of Patent: September 4, 2007Assignee: Phyton, Inc.Inventors: Venkataraman Bringi, Prakash G. Kadkade, Christopher L. Prince, Braden L. Roach
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Patent number: 7238862Abstract: The present invention relates to an improved transformation and regeneration system for wheat. In particular, the invention relates to enhancements in the glyphosate selection system. The transformation method is efficient and reliable for production of fertile plants with improved agronomic qualities.Type: GrantFiled: August 22, 2002Date of Patent: July 3, 2007Assignee: Monsanto Technology LLCInventors: Helen Allison, Xiaorong Feng, Joyce E. Fry, Tianci Hu, Fengming Lu, Maxim Radionenko
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Patent number: 7238527Abstract: The present disclosure relates to methods and compositions for in vitro cultivation of species of Swertia, e.g. Swertia chirata (Ham.). The disclosure provides culture media comprising Murashige and Skoog (MS) basal culture medium, plant hormones preferably selected from the group consisting of benzyladenine (BAP), gibberellic acid (GA3), and auxins, and other additives, e.g. sucrose and agar. Preferably, auxins are selected from the group consisting of indole acetic acid (IAA), indole butyric acid (IBA), and naphthalene acetic acid (NAA). Individual plant hormone concentrations are preferably from about 0.5 mg/L to about 5.0 mg/L.Type: GrantFiled: August 13, 2004Date of Patent: July 3, 2007Inventors: Ashok Ahuja, Sushma Koul, Brij Lal Kaul, Navin Kumar Verma, Mahraj Krishen Kaul, Ravinder Kumar Raina, Ghulam Nabi Qazi
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Patent number: 7235402Abstract: A bioreactor culture system and process for producing conifer somatic embryos comprise a closed vessel, a biomass immobilization matrix, a liquid culture medium recirculating equipment, and a gas control equipment. The biomass immobilization matrix is installed in a closed vessel, a liquid culture medium is introduced in the closed vessel, and the level of liquid culture medium in the closed vessel is kept lower than the biomass immobilization matrix. The liquid culture medium recirculating equipment sprays liquid culture medium from the closed vessel onto the biomass immobilization matrix to thereby irrigate the maturing immobilized biomass.Type: GrantFiled: March 21, 2005Date of Patent: June 26, 2007Assignee: Universite LavalInventors: Dany Aubry, Jean Archambault, Francine M. Tremblay
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Patent number: 7189568Abstract: The present invention provides a method of clonal propagation of Pandanus amaryllifolius and further provides a composition for the clonal propagation of scented Pandanus amaryllifolius.Type: GrantFiled: November 12, 2004Date of Patent: March 13, 2007Assignee: Council of Scientific and Industrial ResearchInventors: Bhagyalakshmi Neelwarne, Thimmaraju Rudrappa, Mandayam Singara Narayan, Gokare Aswathanarayana Ravishankar
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Patent number: 7157620Abstract: The present invention relates to methods for the transformation and regeneration of transformed embryogenic tissue of coniferous plants. In particular, the invention relates to improved methods for transforming embryogenic tissue of coniferous plants and for regenerating transformed embryogenic tissue of coniferous plants. The invention is well suited to the transformation and regeneration of transformed embryogenic tissue of plants of the subgenus Pinus of pines and hybrids thereof.Type: GrantFiled: October 10, 2001Date of Patent: January 2, 2007Assignee: Mead Westvaco CorporationInventors: Marie B. Connett-Porceddu, Heather J. Gladfelter, Jon E. Gulledge, Ryan R. McCormack
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Patent number: 7151203Abstract: Disclosed are methods for producing grape plants having resistance to bunch rot disease or Botrytis or both. Also disclosed are grape plants having resistance to bunch rot disease or Botrytis or both, wherein the plants express a cecropin B peptide Shiva-1.Type: GrantFiled: May 19, 2003Date of Patent: December 19, 2006Assignees: University of Florida Research Foundation, Inc., The United States of America as represented by the Secretary of AgriculureInventors: Ralph Scorza, Dennis J. Gray
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Patent number: 7145058Abstract: The present invention relates to an efficient and cost effective method of preventing growth of genetic transformant bacteria Agrobacterium tumefaciens after transformation in plants by using tea leaf extract as a bactericide, wherein said method leads to elimination of common problem of polyphenol oxidation during transformation and thereby helps maintain regeneration potential in explants and also helps in increased transformation efficacyType: GrantFiled: March 27, 2002Date of Patent: December 5, 2006Assignee: Council of Scientific and Industrial ResearchInventors: Indra Sandal, Amita Bhattacharya, Ashu Gulati, Srigiripuram D. Ravindranath, Paramvir Singh Ahuja
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Patent number: 7129394Abstract: The present invention relates to Production of transgenic tea (Camellia sinensis (L.) O. Kuntze) through biolistic.Type: GrantFiled: January 22, 2002Date of Patent: October 31, 2006Assignee: Council of Scientific and IndustrialInventors: Indra Sandal, Amita Bhattacharya, Paramvir Singh Ahuja
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Patent number: 7102056Abstract: Improved compositions and methods for transformation and regeneration of plants from embryogenic callus are disclosed that include, for example: use of an intermediate-incubation medium after callus induction to increase the competence of the transformed cells for regeneration; dim light conditions during early phases of selection; use of green callus tissue as a target for microprojectile bombardment; and media with optimized levels of phytohormones and copper concentrations.Type: GrantFiled: April 18, 2000Date of Patent: September 5, 2006Assignee: The Regents of the University of CaliforniaInventors: Peggy G. Lemaux, Myeong-Je Cho
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Patent number: 7090996Abstract: A process for the examination of biocompartments (1), having a micro-flow chamber (2) which contains a biocompartment (1) which is continually or intermittently subjected to the through-flow of a culture medium. In a culture medium zone proximal to the biocompartments (1), an electrical potential is applied in such a manner, that from a substance in the culture medium which is released or consumed by the biocompartments (1), OH? and/or H+ ions are formed. During the application of the potential, a first measurement (pH?1, pH?3) for the pH value of the culture medium is measured. The potential is then switched off or changed in such a manner that the formation of OH? and/or H+ ions from the said substance is stopped. Before or after the measuring of the first measured value, (pH?1, pH?3) with switched off electrical potential, a second measured value (pH?2, pH?4) is measured for the pH value of the culture medium.Type: GrantFiled: September 7, 2004Date of Patent: August 15, 2006Assignee: Micronas GmbHInventor: Mirko Lehmann
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Patent number: 7045354Abstract: The present invention relates to methods for semi-continuous culture of plant cells in a nutrient medium. The pH of the medium is monitored during the cell culture as a way of monitoring the expression of a product of interest by the cells.Type: GrantFiled: November 14, 2001Date of Patent: May 16, 2006Assignee: The Regents of the University of CaliforniaInventors: Karen A. McDonald, Alan P. Jackman, Melody M. Trexler
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Patent number: 7015032Abstract: A plant micropropagation apparatus and process is provided in which a support platform for vessels containing a liquid growth media are periodically pivoted which brings about an intermittent immersion of the plant tissue/growth substrate in the growth media. The motion of the support platform may optionally be used to engage a piston operated dispenser for supplying aseptic media to the sealed vessel. The intermittent immersion also provides for an improved method of separating viable embryos from culture materials using a separation matrix in conjunction with the intermittent wave motion of the plant media when suspended in a liquid culture.Type: GrantFiled: March 25, 2004Date of Patent: March 21, 2006Assignee: Clemson UniversityInventors: Jeffrey W. Adelberg, E. Phillip Simpson
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Patent number: 6905876Abstract: The present disclosure relates to methods and compositions for in vitro cultivation of species of Polygonatum, e.g. Polygonatum cirrhifolium Royle. The disclosure provides culture media comprising MS basal culture media and plant hormones, preferably selected from the group consisting of gibberellic acid (GA3), 6-benzyl-aminopurine (BAP), and naphthalene acetic acid (NAA). The disclosure provides methods of in vitro cultivation of Polygonatum comprising contacting Polygonatum seeds with a first medium comprising MS basal culture medium and GA3, upon emergence of a hypocotyl, transferring this primary explant to a second medium comprising MS basal culture medium, BAP, and NAA, and upon emergence of a first foliage leaf, transferring this secondary explant to a third medium comprising MS basal culture medium, BAP, NAA, and gibberellic acid (GA3).Type: GrantFiled: November 16, 2001Date of Patent: June 14, 2005Assignee: Council of Scientific and Industrial ResearchInventors: Ghulam Nabi Qazi, Surrinder Kumar Lattoo, Avtar Krishan Dhar, Paresh Purohit, Ravinder Kumar Raina, Rekha Sapru Dhar
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Patent number: 6900057Abstract: Improved methods for the regeneration of transgenic plants using apical dominance inhibitors are disclosed. Use of dikegulac to induce multiple shoot formation in culture is particularly disclosed.Type: GrantFiled: January 16, 2002Date of Patent: May 31, 2005Assignee: Monsanto Technology LLCInventors: John Austin Burns, Lori A Smith, Dannette C Ward
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Patent number: 6858777Abstract: Multiple shoot structures are induced from plant tissues (e.g., shoot apices or axillary buds on an artificial medium) to produce multiple shoot cultures. These multi-shoot cultures are then transformed by known transformation methods. Plants are subsequently regenerated from the transformed cells. Crops that may be efficiently transformed by this method include plants normally recalcitrant to transformation such as sugar beet, sunflower, soybean, cotton, tobacco, tomato, peanuts, melons, watermelon, squash, Brassica, and pepper.Type: GrantFiled: August 13, 2001Date of Patent: February 22, 2005Assignee: Syngenta Participations AGInventors: Heng Zhong, Eric Boudreau, Sabrina Rouse, Erik Dunder, Weining Gu, Yin-Fu Chang
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Patent number: 6846971Abstract: The present invention is directed to a method of Acacia mangium regeneration through organogenesis and a method of genetic transformation of Acacia mangium. The method of regeneration comprises inducing callus from different parts of seedlings and vegetatively micropropagated plantlets as explants; adventitious bud induction followed by pinnate leaf and bud elongation and eventually, elongated shoots were induced to root. Based on the regeneration system, a marker gene GUS under cauliflower mosaic virus promoter was introduced to Acacia mangium via Agrobacterium infection. GUS staining in the regenerated plants and Southern blot hybridization prove the incorporation of the foreign gene into the host genome and expression of the foreign gene.Type: GrantFiled: January 19, 2000Date of Patent: January 25, 2005Assignee: Temasek Life Sciences Laboratory LimitedInventors: Deyu Xie, Yan Hong
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Publication number: 20040191908Abstract: The present invention provides a method of clonal propagation of Pandanus amaryllifolius and further provides a composition for the clonal propagation of scented Pandanus amaryllifolius.Type: ApplicationFiled: March 27, 2003Publication date: September 30, 2004Applicant: Council of Scientific and Industrial ResearchInventors: Bhagyalakshmi Neelwarne, Thimmaraju Rudrappa, Mandayam Singara Narayan, Gokare Aswathanarayana Ravishankar
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Publication number: 20040187177Abstract: The invention provides a method to enhance Agrobacterium-mediated transformation of plant cells, parts and tissues, thereby enhancing the production of transgenic plants.Type: ApplicationFiled: March 30, 2004Publication date: September 23, 2004Applicant: Regents of the University of MinnesotaInventors: Paula Olhoft, David A. Somers
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Patent number: 6764854Abstract: The present invention provides methods for generating doubled haploid and/or haploid plants from microspores. In a presently preferred embodiment of the methods of the present invention, plant material is selected that bears reproductive organs containing microspores at a developmental stage that is amenable to androgenic induction. The microspores are treated by contacting the selected plant material with water and subjecting the selected plant material to temperature stress, and optionally to nutrient stress. Preferably the selected plant material is contacted with an effective amount of a sporophytic development inducer and an effective amount of an auxin and/or a cell spindle inhibiting agent. Optionally, the selected plant material is contacted with an effective amount of a cytokinin and/or an effective amount of a gibberellin. The treated microspores are isolated, preferably by density centrifugation utilizing a solution of 0.Type: GrantFiled: January 8, 2002Date of Patent: July 20, 2004Assignee: Northwest Plant Breeding CompanyInventors: Calvin F. Konzak, Enrique A. Polle, Weiguo Liu, Yuanming Zheng
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Patent number: 6753459Abstract: Genetic constructs, transformation vectors and methods are taught for production of transgenic plants which can be selectively removed from a growing site by application of a chemical agent or physiological stress. The invention links a target gene for the trait of commercial interest to a conditionally lethal gene, which can be selectively expressed to cause plant death. By use of the genetic constructs, transformation vectors and methods of the present invention, invasion of environments and contamination of commercial non-engineered productions by transgenic plants can be avoided. Methods are also taught for transformation of Brassica species.Type: GrantFiled: June 22, 2001Date of Patent: June 22, 2004Assignees: National Research Council of Canada, Dow Agrosciences LLCInventors: Wilfred A. Keller, Steven F. Fabijanski, Paul G. Arnison, Joseph K. Hammerlindl, Steven R. Webb
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Patent number: 6753463Abstract: A transformed cotton plant. The transformed cotton plant comprises DNA derived from a source other than cotton plants, wherein the DNA, when transformed into the cotton plants, confers a phenotype not expressed inn a parent cotton.Type: GrantFiled: November 9, 1994Date of Patent: June 22, 2004Assignee: Mycogen CorporationInventors: Thirumale S. Rangan, David M. Anderson, Kanniah Rajasekaran, John W. Grula, Richard Lorne Hudspeth, Richard L. Yenofsky
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Publication number: 20040106199Abstract: The invention provides cyclodextrin derivatives that are substituted with groups bearing charges in aqueous solutions (charged cyclodextrins) in their salt forms and their use, also in combinations with other cyclodextrins, as useful components of plant cell and tissue growth media. The invention also comprises a new method of isolation of useful hydrophobic compounds, such as taxol, produced by plant cultures from the cyclodextrin-containing growth media and from the corresponding cell cultures.Type: ApplicationFiled: December 2, 2002Publication date: June 3, 2004Inventor: Alexey V. Eliseev
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Patent number: 6740526Abstract: The subject invention is related to a method for recombinantly and transiently producing a polypeptide in a plant tissue, which includes providing a plant tissue sample in a bioreactor; adding to the plant tissue sample, a sample of Agrobacterium containing a nucleotide sequence encoding the polypeptide on the T-DNA, co-culturing the plant tissue sample with the Agrobacterium so that the nucleotide sequence is transferred to the plant, allowing the plant tissue to transiently express the polypeptide, and then separating the polypeptide from the mixture.Type: GrantFiled: September 22, 2000Date of Patent: May 25, 2004Inventor: Wayne R. Curtis
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Patent number: 6682931Abstract: This invention relates to a method for improving the growth and regeneration potential of embryogenic cell and tissue cultures of coniferous plants retrieved from cryopreservation. In particular, this invention relates to the use of abscisic acid in the post-cryopreservation recovery medium to improve both the growth and somatic embryo production of embryogenic cell and tissue cultures of conifers, thereby enabling more rapid proliferation of the embryogenic cultures and a subsequent increase in the yield of somatic embryos. This method is well-suited for employment with a number of biotechnological uses of embryogenic cultures of coniferous plants retrieved from cryopreservation, including use with embryogenic cultures of coniferous plants and with genetically transformed embryogenic cultures of coniferous plants for producing clonal planting stock useful for reforestation.Type: GrantFiled: May 19, 2000Date of Patent: January 27, 2004Assignee: MeadWestvaco CorporationInventors: Michael Ryan Becwar, Sharon Anne Krueger
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Patent number: 6677154Abstract: The invention consists of several novel solutions to overcome specific problems currently encountered in propagating bamboo. The invention consists of these novel solutions both individually and in combination.Type: GrantFiled: February 18, 1999Date of Patent: January 13, 2004Inventors: Johan Gielis, John E. Woods, Susan H. Woods, Jan Oprins
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Patent number: 6660910Abstract: This invention is concerned with the use of cyanamide hydratase as a selection marker in plant transformation. Cyanamide acts as a herbicide and plants transformed with the gene coding for cyanamide hydratase are able to convert the cyanamide into urea which enables the selection of transformed plants by survival under cyanamide pressure.Type: GrantFiled: October 15, 1999Date of Patent: December 9, 2003Assignee: Syngenta Mogen B.V.Inventor: Brigitte Damm
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Patent number: 6646185Abstract: The present invention provides an efficient genetic transformation system for the zoysiagrass plant (Zoysia japonica Steud.). Also provided are optimized media compositions and culture conditions for the zoysiagrass transformation. The reliable transformation system for zoysiagrass was developed by optimizing several factors that significantly affect calli growth and plant regeneration. Callus type and co-cultivation period absolutely influenced the transformation efficiency. Concentrations of 2,4-D, CaCl2 and acetosyringone were also critical factors. The best result was achieved when type 3 calli were co-cultivated on a 2,4-D-free co-cultivation medium for 6 days. Removal of calcium and addition of 50 mg/liter acetosyringone during co-cultivation drastically enhanced the efficiency. The invention also provides a bialaphos-resistant zoysiagrass plant, which can be used in golf courses and athletic fields to save the maintenance cost.Type: GrantFiled: November 20, 2001Date of Patent: November 11, 2003Assignee: Korea Kumho Petrochemical Co., Ltd.Inventors: Tohyama Kohichi, Chang-Hyu Bae, Jeong-Gu Kang, Pill-Soon Song, Chung-Mo Park, Hyo Yeon Lee
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Patent number: 6642437Abstract: Methods for producing proteins in plant seeds are disclosed. Expression of the protein is driven by a seed-specific promoter and the protein is preferably expressed as a fusion polypeptide that includes a signal peptide that causes the protein to accumulate in a subcellular compartment to protect the protein.Type: GrantFiled: September 30, 1998Date of Patent: November 4, 2003Assignee: The Regents of the University of CaliforniaInventors: Peggy G. Lemaux, Myeong-Je Cho, Bob B. Buchanan
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Patent number: 6638755Abstract: A simple culture medium produced by impregnating a fibrous water-absorbent sheet with a suspension formed by suspending in an alcohol (a) an adhesive (0.01-0.4 wt. %) which is soluble in both water and alcohol, (b) a gelling agent which is soluble in water and insoluble in alcohol, and (c) a bacterial nutritive ingredient, the fibrous water-absorbent sheet having a mesh larger than the particle size of the gelling agent and being placed on a waterproof flat plate, and by drying the resultant sheet while suppressing rapid evaporation of the alcohol, to thereby cause the water-absorbent sheet to adhere onto the waterproof flat plate; and a method for producing the medium.Type: GrantFiled: November 19, 2001Date of Patent: October 28, 2003Assignee: Nissui Pharmaceutical Co., Ltd.Inventors: Shingo Mizuochi, Hidemasa Kodaka, Hideaki Shibata
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Patent number: 6632666Abstract: Gel-based medium compositions and a method of use thereof in normothermic, hypothermic or cryopreservative storage and transport of cell samples are described. These gel-based compositions contain a cell maintenance and preservation medium together with a gelling agent. Such gel-based medium compositions protect various cell samples, such as animal or plant organs, tissues and cells, from the mechanical, physiological and biochemical stresses inherently associated with liquid preservation techniques.Type: GrantFiled: January 11, 2001Date of Patent: October 14, 2003Assignee: BioLife Solutions, Inc.Inventors: John M. Baust, Robert Van Buskirk, John G. Baust