By Use Of Intercalating Agent (e.g., Acridine Orange, Etc.) Patents (Class 435/443)
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Publication number: 20120244584Abstract: The present disclosure provides, inter alia, Thraustochytrium and relevant methods and reagents, including engineered regulatory sequences from and/or operative in Thraustochytrid or Thraustochytrium, selectable markers useful for engineering microorganisms such as Thraustochytrids, means for mutagenizing microorganisms, novel strains produced by mutagenesis, and methods and compositions related to production of particular compounds in microorganisms.Type: ApplicationFiled: March 7, 2012Publication date: September 27, 2012Applicant: OCEAN NUTRITION CANADA LIMITEDInventors: ShuoCheng Zhang, Roberto Armenta
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Patent number: 8101386Abstract: The present invention relates to the fields of microbiology and microbial genetics. More specifically, the invention relates to novel bacteria strains and processes employing these strains for the fermentative production of amino acids such as threonine.Type: GrantFiled: June 28, 2010Date of Patent: January 24, 2012Assignee: Archer Daniels Midland CompanyInventors: Hungming J. Liaw, Jill S. Bradshaw, Yueqin Yang, Weiying Mao
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Patent number: 7927606Abstract: Free-living microbes are provided in which the nucleic acid has been modified so that the microbe is attenuated for proliferation and/or which comprise genetic mutations that attenuate the ability of the microbe to repair its nucleic acid. Methods of using the modified microbes for the loading, activation, and/or maturation of antigen-presenting cells are also provided. Vaccine compositions comprising the modified microbes and/or the antigen-presenting cells and methods of using the vaccines are also provided. The microbes may be further modified to include heterologous antigens, such as tumor antigens or infectious disease antigens, for use as a vaccine against cancer or infectious diseases.Type: GrantFiled: August 11, 2006Date of Patent: April 19, 2011Assignee: Aduro BiotechInventors: Thomas W. Dubensky, Jr., Dirk G. Brockstedt, Keith S. Bahjat, John E. Hearst, David N. Cook, William S. Luckett, Jr.
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Patent number: 7842289Abstract: The present invention provides recombinant nucleic acid molecules, expression cassettes, and vectors useful for expression of polypeptides, including heterologous polypeptides, such as antigens, in bacteria. Some of the recombinant nucleic acid molecules, expression cassettes and vectors comprise codon-optimized sequences encoding the polypeptides and/or signal peptides. Some of the recombinant nucleic acid molecules, expression cassettes, and expression vectors comprise sequences encoding non-Listerial and/or non-secA1 signal peptides for secretion of the polypeptides. The invention also provides bacteria comprising the nucleic acid molecules, expression cassettes, and expression vectors, as well as compositions such as vaccines comprising the bacteria. Methods of making and using the bacteria, recombinant nucleic acid molecules, and expression cassettes are also provided.Type: GrantFiled: December 23, 2004Date of Patent: November 30, 2010Assignee: Aduro BioTechInventors: Thomas W. Dubensky, Jr., Daniel A. Portnoy, William S. Luckett, Jr., David N. Cook
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Patent number: 7767431Abstract: The present invention relates to the fields of microbiology and microbial genetics. More specifically, the invention relates to novel bacteria strains and processes employing these strains for the fermentative production of amino acids such as threonine.Type: GrantFiled: April 13, 2007Date of Patent: August 3, 2010Assignee: Archer-Daniels-Midland CompanyInventors: Hungming J. Liaw, Jill S. Bradshaw, Yueqin Yang, Weiying Mao
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Patent number: 7695725Abstract: Free-living microbes are provided in which the nucleic acid has been modified so that the microbe is attenuated for proliferation and/or which comprise genetic mutations that attenuate the ability of the microbe to repair its nucleic acid. Methods of using the modified microbes for the loading, activation, and/or maturation of antigen-presenting cells are also provided. Vaccine compositions comprising the modified microbes and/or the antigen-presenting cells and methods of using the vaccines are also provided. The microbes may be further modified to include heterologous antigens, such as tumor antigens or infectious disease antigens, for use as a vaccine against cancer or infectious diseases.Type: GrantFiled: June 30, 2004Date of Patent: April 13, 2010Assignee: Aduro BiotechInventors: Thomas W. Dubensky, Jr., Dirk G. Brockstedt, John E. Hearst, David N. Cook, William S. Luckett, Jr.
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Patent number: 7687253Abstract: The present invention relates to the fields of microbiology and microbial genetics. More specifically, the invention relates to novel bacteria strains and processes employing these strains for the fermentative production of amino acids such as threonine.Type: GrantFiled: March 19, 2008Date of Patent: March 30, 2010Assignee: Archer-Daniels-Midland CompanyInventors: Hungming J. Liaw, Jill S. Bradshaw, Yueqin Yang, Weiying Mao
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Publication number: 20090298151Abstract: The disclosed invention relates to an isolated hydrogen gas producing microorganism, termed Enterobacter sp. SGT-T4™ and derivatives thereof. Compositions and methods comprising the disclosed microorganisms are also provided.Type: ApplicationFiled: June 3, 2008Publication date: December 3, 2009Applicant: Dr. Elmar Schmid and James GibsonInventors: Elmar Schmid, James Gibson
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Publication number: 20090029433Abstract: The disclosed invention relates to an isolated hydrogen gas producing microorganism, termed Enterobacter sp. SGT 06-1™.Type: ApplicationFiled: July 27, 2007Publication date: January 29, 2009Applicant: Dr. Elmar Schmid and James GibsonInventors: Elmar Schmid, James Warner Gibson
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Patent number: 7303897Abstract: The invention relates to a fragmentation process that depends on mismatches between two strands of parental polynucleotides. One embodiment, comprises a method for preparing polynucleotide fragments of DNA comprising formation of heteroduplex molecules by hybridizing polynucleotides. The invention also provides a method and process of forming fragments which can be used with any shuffling process or combination of shuffling processes.Type: GrantFiled: November 17, 2003Date of Patent: December 4, 2007Assignee: Proteus S.A.Inventors: Daniel Dupret, Fabrice Lefevre, Laurent Fourage
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Patent number: 7220571Abstract: The present invention relates to the fields of microbiology and microbial genetics. More specifically, the invention relates to novel bacterial strains and processes employing these strains for the fermentative production of amino acids such as threonine.Type: GrantFiled: September 26, 2001Date of Patent: May 22, 2007Assignee: Archer-Daniels-Midland CompanyInventors: Hungming J Liaw, Jill S Bradshaw, Yueqin Yang, Weiying Mao
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Patent number: 7208317Abstract: Cellular libraries useful for in vitro phenotyping and gene mapping. In a representative approach, a method for preparing a homozygous cellular library includes the steps of providing a heterozygous cellular library comprising a plurality of isolated parent cells; inducing site-specific mitotic recombination in the plurality of isolated parent cells; culturing the plurality of isolated parent cells, whereby a population of daughter cells is produced; and selecting daughter cells comprising a homozygous genetic modification, whereby a homozygous cellular library is prepared.Type: GrantFiled: May 2, 2003Date of Patent: April 24, 2007Assignee: The University of North Carolina at Chapel HillInventors: David W. Threadgill, Daekee Lee
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Patent number: 7078389Abstract: A mutagenic, triplex-forming oligonucleotide and methods for use thereof wherein the oligonucleotide is chemically modified to incorporate a mutagen and forms a triple-stranded nucleic acid molecule with a specific DNA segment of a target DNA molecule. Upon formation of the triplex, the mutagen is brought into proximity with the target molecule and causes a mutation at a specific site therein. The mutation activates, inactivates or alters the activity and function of the target molecule.Type: GrantFiled: February 14, 2001Date of Patent: July 18, 2006Assignee: Yale UniversityInventors: Peter M. Glazer, Pamela A. Havre
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Patent number: 6922850Abstract: Visor assembly (1, 21) consisting of an inner shield (6, 26) and an outer shield (2, 22). In the use position these shields are some distance apart. With this arrangement the inner shield (6, 26) is located within the peripheral confines of the outer shield (2, 22). The inner shield (6, 26) is provided with an elevation (7, 27) which extends close to the periphery thereof and which acts as a spacer with respect to the outer shield. This elevation (7, 27) is preferably made of silicone material which is snack to the inner shield (6, 26) but is not stuck to the outer shield (2, 22). Fixing to the outer shield (2, 22) takes place by means (10, 12) of mechanical fixing means.Type: GrantFiled: August 24, 2000Date of Patent: August 2, 2005Assignee: Derek's Patent B.V.Inventor: Derek Leslie Arnold
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Patent number: 6835867Abstract: The present invention relates to methods of producing an allelic series of modifications in genes of interest in a cell. In particular, the invention provides methods for using nucleic acid sequence-modifying agents (e.g., chemicals, electromagnetic radiation, etc.) to introduce modifications in any nucleic acid sequence in the genome of a cell. Also provided are sets of cells which contain at least one modification in any gene of interest. The methods and compositions of the invention are useful in determining the function of the gene of interest.Type: GrantFiled: June 24, 1998Date of Patent: December 28, 2004Inventors: Richard P. Woychik, Terry R. Magnuson, Ellis D. Avner, James W. Thomas
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Patent number: 6696294Abstract: The present invention provides methods for generating and identifying mutations in any target gene of a polyploid plant species. In a preferred aspect of the present invention, a plant is constructed and/or selected that has at least one copy of a functional, target gene located exclusively in only one of its homoeologous, or homologous, genomes. Seed derived from the selected plant are then contacted with an effective amount of at least one mutagenic agent, the treated seed are germinated and the seeds or plants derived therefrom, are screened for mutations in the target gene. Thus, the inventive concepts set forth herein can be used to create, select and identify mutations in any target gene of any suitable polyploid plant, thereby providing a source of numerous, readily-identifiable mutations that can, if so desired, be used in crosses to develop unique new crop cultivars.Type: GrantFiled: December 18, 2000Date of Patent: February 24, 2004Assignee: Northwest Plant Breeding Co.Inventor: Calvin F. Konzak
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Publication number: 20030113759Abstract: Disclosed is a process of performing “sexual” PCR which includes generating random polynucleotides by interrupting or blocking a synthesis or amplification process to show or halt synthesis or amplification of at least one polynucleotide, optionally amplifying the polynucleotides, and reannealing the polynucleotides to produce random mutant polynucleotides. Also provided are vector and expression vehicles including such mutant polynucleotides, polypeptides expressed by the mutant polynucleotides and a method for producing random mutant polypeptides.Type: ApplicationFiled: August 12, 2002Publication date: June 19, 2003Inventor: Jay M. Short
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Publication number: 20030068808Abstract: The invention provides methods for generating antibiotic resistant bacteria comprising blocking mismatch repair in a bacterium to make hypermutable bacteria, contacting the bacteria with at least one antibiotic, selecting bacteria that are resistant to the antibiotic, and culturing the antibiotic resistant bacteria. The invention also provides methods of determining the genes responsible for antibiotic resistance.Type: ApplicationFiled: July 25, 2001Publication date: April 10, 2003Inventors: Nicholas C. Nicolaides, Philip M. Sass, Luigi Grasso, J. Bradford Kline
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Publication number: 20020106800Abstract: The present invention relates to the fields of microbiology and microbial genetics. More specifically, the invention relates to novel bacterial strains and processes employing these strains for the fermentative production of amino acids such as threonine.Type: ApplicationFiled: September 26, 2001Publication date: August 8, 2002Inventors: Hungming J. Liaw, Jill S. Bradshaw, Yueqin Yang, Weiying Mao
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Publication number: 20020068053Abstract: The invention relates to a pharmaceutical preparation comprising tumor-reactive cells similar to Th1 which produce large quantities of interferon &ggr; and produce little or no interleukin 4; together with pharmaceutically compatible excipients and auxiliary agents. The invention also relates to a method for producing tumor-reactive cells similar to Th1 which produce large quantities of interferon &ggr; and produce little or no interleukin 4.Type: ApplicationFiled: August 16, 2001Publication date: June 6, 2002Inventors: Martin Rocken, Oliver Egeter, Ralph Mocikat
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Publication number: 20020028922Abstract: A mutagenic, triplex-forming oligonucleotide and methods for use thereof wherein the oligonucleotide is chemically modified to incorporate a mutagen and forms a triple-stranded nucleic acid molecule with a specific DNA segment of a target DNA molecule. Upon formation of the triplex, the mutagen is brought into proximity with the target molecule and causes a mutation at a specific site therein. The mutation activates, inactivates or alters the activity and function of the target molecule.Type: ApplicationFiled: February 14, 2001Publication date: March 7, 2002Inventors: Peter M. Glazer, Pamela A. Havre
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Patent number: 6303376Abstract: A high affinity, triplex-forming oligonucleotide and methods for use thereof wherein an oligonucleotide is used to form a triple-stranded nucleic acid molecule with a specific DNA segment of a target DNA molecule. Upon formation of the triplex, the binding of the oligonucleotide stimulates mutagenesis within or adjacent to the target sequence using cellular DNA synthesis or repair mechanisms. The mutation activates, inactivates or alters the activity and function of the target molecule.Type: GrantFiled: October 4, 1999Date of Patent: October 16, 2001Assignee: Yale UniversityInventor: Peter M. Glazer
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Patent number: 6300317Abstract: This invention provides a self-assembling polynucleotide delivery system comprising components aiding in the delivery of the polynucleotide to the desired address which are associated via noncovalent interactions with the polynucleotide. The components of this system include DNA-masking components, cell recognition components, charge-neutralization and membrane-permeabilization components, and subcellular localization components. Specific compounds useful in this system are also provided.Type: GrantFiled: June 6, 1995Date of Patent: October 9, 2001Assignee: The Regents of the University of CaliforniaInventors: Francis C. Szoka, Jr., Jean Haensler
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Patent number: 6271007Abstract: The invention relates to novel yeast strains having a reduced ability to metabolize xylitol. The invention further relates to the use of said strains for the production of xylitol.Type: GrantFiled: February 7, 1994Date of Patent: August 7, 2001Assignee: Xyrofin OyInventors: Juha Apajalahti, Matti Leisola
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Patent number: 5955365Abstract: This invention provides a self-assembling polynucleotide delivery system comprising components aiding in the delivery of the polynucleotide to the desired address which are associated via noncovalent interactions with the polynucleotide. The components of this system include DNA-masking components, cell recognition components, charge-neutralization and membrane-permeabilization components, and subcellular localization components. Specific compounds useful in this system are also provided.Type: GrantFiled: June 7, 1995Date of Patent: September 21, 1999Assignee: Regents of the University of CaliforniaInventors: Francis C. Szoka, Jr., Jean Haensler
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Patent number: 5795802Abstract: A method for manufacturing a semiconductor device, the method includes the steps of forming an n-type well and a p-type well under a surface of a semiconductor substrate, forming a pad oxide layer having a first thickness on the p-type well and a second thickness on the n-type well, the first thickness being greater than the second thickness, and forming a field oxide layer between the n-type well and the p-type well, the field oxide layer having less bird's beak on the n-type well than on the p-type well.Type: GrantFiled: September 3, 1997Date of Patent: August 18, 1998Assignee: LG Semicon Co., Ltd.Inventors: Sang-Gi Ko, Mun-Mo Jeong
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Patent number: RE44250Abstract: Visor assembly (1, 21) consisting of an inner shield (6, 26) and an outer shield (2, 22). In the use position these shields are some distance apart. With this arrangement the inner shield (6, 26) is located within the peripheral confines of the outer shield (2, 22). The inner shield (6, 26) is provided with an elevation (7, 27) which extends close to the periphery thereof and which acts as a spacer with respect to the outer shield. This elevation (7, 27) is preferably made of silicone material which is snack to the inner shield (6, 26) but is not stuck to the outer shield (2, 22). Fixing to the outer shield (2, 22) takes place by means (10, 12) of mechanical fixing means.Type: GrantFiled: August 24, 2000Date of Patent: June 4, 2013Assignee: Derek's Patent B.V.Inventor: Derek Leslie Arnold