Involving Laser Treatment Of The Cell Before Or During Transfection Patents (Class 435/460)
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Patent number: 11191278Abstract: A method of delivering at least one nucleic acid to a plant, comprising: applying a light energy to a surface of the plant to create a pore; and applying at least one nucleic acid to the pore, wherein the at least one nucleic acid comprises RNA, wherein the at least one nucleic acid comprises at least one of a RNA interference (RNAi), an antisense RNA (asRNA), a micro RNA (miRNA), a small interfering RNA (siRNA), a double-stranded RNA (dsRNA), a non-coding RNA (ncRNA), mitochondrial RNA (mtDNA), and combinations thereof.Type: GrantFiled: December 21, 2018Date of Patent: December 7, 2021Assignee: Premier Citrus ApZ, LLCInventor: Gregory P Drouillard
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Patent number: 10278334Abstract: A method of delivering at least one nucleic acid to a plant, comprising: applying a light energy to a surface of the plant to create a pore; and applying at least one nucleic acid to the pore, wherein the at least one nucleic acid comprises RNA, wherein the at least one nucleic acid comprises at least one of a RNA interference (RNAi), an antisense RNA (asRNA), a micro RNA (miRNA), a small interfering RNA (siRNA), a double-stranded RNA (dsRNA), a non-coding RNA (ncRNA), mitochondrial RNA (mtDNA), and combinations thereof.Type: GrantFiled: March 24, 2017Date of Patent: May 7, 2019Assignee: PREMIER CITRUS APZ, LLCInventor: Gregory P. Drouillard
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Patent number: 9840714Abstract: A method for gene introduction by which higher efficiency for gene introduction than that by the conventional Agrobacterium method may be attained simply and without injuring the tissue is disclosed. According to the method of the present invention, the efficiency of gene introduction into plant cells by a bacterium belonging to genus Agrobacterium is promoted by accompanying centrifugation of the plant cells or plant tissue.Type: GrantFiled: May 3, 2011Date of Patent: December 12, 2017Assignee: JAPAN TOBACCO INC.Inventors: Yukoh Hiei, Keisuke Kasaoka, Yuji Ishida
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Patent number: 8865450Abstract: The present invention relates to a method for production of continuous cell lines comprising providing living cells of an animal or a human, irradiating said cells with UV light, proliferating said cells and selecting multiplying cells as cells of a continuous cell line.Type: GrantFiled: February 20, 2009Date of Patent: October 21, 2014Assignees: Baxter International Inc., Baxter Healthcare S.A.Inventors: Manfred Reiter, Wolfgang Mundt, Simone Feigl, Simone Von Fircks
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Patent number: 8785742Abstract: Disclosed is the seed of a novel soybean cultivar, designated 1000729, a sample of which is deposited under ATCC Accession No. PTA-121009. Also disclosed are plants, or parts thereof, grown from the seed of the cultivar, plants having the morphological and physiological characteristics of the 1000729 cultivar, and methods of using the plant or parts thereof in a soybean breeding program.Type: GrantFiled: November 13, 2012Date of Patent: July 22, 2014Assignee: Agrigenetics, Inc.Inventor: Hunt B. Wiley
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Patent number: 8778682Abstract: A method of delivering exogenous molecules, comprising: providing a plurality of cells having a cell membrane; adding a plurality of exogenous molecules to the cells; exposing the cells to a defocused infrared (IR) light to permeabilize the cell membrane of the cells; and delivering the exogenous molecules to the cells through the permeablized cell membrane, wherein an intensity of the IR light at the optical focus is at least greater than or equal to an order of 104 W/cm2.Type: GrantFiled: August 30, 2011Date of Patent: July 15, 2014Assignee: General Electric CompanyInventors: Vasile Bogdan Neculaes, Kenneth Roger Conway, Allen Lawrence Garner, Evelina Roxana Loghin, Siavash Yazdanfar, Dmitry Vladimirovich Dylov, Brian Michael Davis, Chulmin Joo
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Patent number: 8435791Abstract: A highly controlled and precise system, device and method for tissue and cellular alteration and treatment below or at surfaces with a laser. The present invention is characterized by ultra low levels of collateral damage as defined by physiologically relevant tests that measure tissue viability. The operation of the present invention is based on spectrally confining the interaction between laser energy and a targeted tissue including an essential element for physiologically relevant tests for monitoring tissue viability.Type: GrantFiled: March 27, 2003Date of Patent: May 7, 2013Assignees: Hadasit Medical Research Services and Development Ltd., Yissum Research Development Company of the Hebrew University of JerusalemInventors: Eithan Galun, Aaron Lewis, Evelyne Zeira, Alexandra Manevitch, Artium Khatchatouriants, Yitzchak Hemo
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Patent number: 8349598Abstract: A method of optoperforation of the membrane of a cell by application of laser pulses characterized by focusing the pulsed laser beam onto the cell membrane to be perforated, applying a series of laser pulses of predetermined pulse energy, measuring the oscillation time of the bubbles formed in the laser focus from the change in laser intensity of a test laser beam transmitted through the laser focus and caused by the bubbles in the laser focus, and increasing the pulse energy to a level at which the oscillation time of the bubbles attains a predetermined value.Type: GrantFiled: January 9, 2008Date of Patent: January 8, 2013Assignee: Universitat Zu LubeckInventor: Alfred Vogel
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Patent number: 8318494Abstract: The presently claimed invention applies to a genetic material processing and manipulation method and related product. The claimed invention relates to a method for changing the inherited characteristics of a cell through chromosome processing treatment. In a preferred embodiment, ‘genomic surgery’ is applied to source genetic material 101. Micro-beam cutting takes place on target genetic material 103, which is moved by way of micro-beam transport to a destination location adjacent to desired target genetic material 105. Source and target genetic material are welded during a micro-beam welding step, resulting in a combined or new chromosome combination 125. The combined or new chromosome combination 125 is transferred into receptor cell by three ways. The presently claimed invention provides a high quality alternate approach to directed genetic recombination without requiring the use of restriction enzymes and is used for chromosomal repair, removal of defects and new organism creation.Type: GrantFiled: November 19, 2009Date of Patent: November 27, 2012Assignee: Geneforge LimitedInventor: Yaoxiong Huang
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Patent number: 8080399Abstract: An opening is formed in a cell using laser radiation. A Bessel beam is formed using the laser radiation and the Bessel beam is directed onto the cell to form an opening. The guiding of material towards the opening may be involved using optical trapping/manipulation. The material may change cellular function or analyse cell behaviour. Both pulsed laser radiation and continuous wave radiation may be formed using the same laser.Type: GrantFiled: November 29, 2005Date of Patent: December 20, 2011Assignee: The University of Court of the University of St. AndrewsInventors: Kishan Dholakia, Christian Thomas Alcuin Brown, Lynn Paterson
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Patent number: 8008077Abstract: The present invention provides a method for introducing a molecule into the cytosol of a cell in which the cell is contacted with a photosensitising agent, the cell is irradiated with light of a wavelength effective to activate the photosensitising agent and, substantially at the same time or after the irradiation, the cell is contacted with the molecule to be introduced, particularly for use in cancer treatment, gene therapy and vaccination.Type: GrantFiled: May 9, 2007Date of Patent: August 30, 2011Assignee: PCI Biotech ASInventors: Kristian Berg, Lina Prasmickaite, Anders Hogset, Pal Kristian Selbo
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Patent number: 8008063Abstract: Electroporation is performed on a population of cells, liposomes, vesicles, or other membrane-encased structures with uniform results regardless of size variations within the population, by drawing the membrane-encased structures into micron-sized openings that contain paired electrodes. An electric potential is then imposed between the paired electrodes to permeabilize only that portion of each cell that extends into the openings and resides within the electric field focused in the area between the electrodes.Type: GrantFiled: June 5, 2009Date of Patent: August 30, 2011Assignee: Bio-Rad Laboratories, Inc.Inventor: Charles W. Ragsdale
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Publication number: 20110159591Abstract: The presently claimed invention applies to a genetic material processing and manipulation method and related product. The claimed invention relates to a method for changing the inherited characteristics of a cell through chromosome processing treatment. In a preferred embodiment, ‘genomic surgery’ is applied to source genetic material 101. Micro-beam cutting takes place on target genetic material 103, which is moved by way of micro-beam transport to a destination location adjacent to desired target genetic material 105. Source and target genetic material are welded during a micro-beam welding step, resulting in a combined or new chromosome combination 125. The combined or new chromosome combination 125 is transferred into receptor cell by three ways. The presently claimed invention provides a high quality alternate approach to directed genetic recombination without requiring the use of restriction enzymes and is used for chromosomal repair, removal of defects and new organism creation.Type: ApplicationFiled: November 19, 2009Publication date: June 30, 2011Inventor: Yaoxiong Huang
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Patent number: 7892837Abstract: The invention relates to an optical method for targeted transfer of molecules, preferably of DNA, RNA, peptides, amino acids and proteins, into vital cells by means of laser radiation and to an arrangement for implementing the method. The object of the invention, to find a novel possibility for targeted molecule transfer into the interior of vital cells, particularly the transfer of DNA, RNA, peptides, amino acids and proteins, which achieves a high transfer efficiency while extensively excluding destructive side effects such as a lethal effect on a treated cell, is met according to the invention in that cellular membranes are opened transiently for the molecule transfer by multiple laser pulses in the microjoule range or less and a pulsed, near-infrared laser beam with a pulse width in the femtosecond range is directed in each instance to a submicrometer spot of a membrane of the vital cell for an irradiation period of less than one second.Type: GrantFiled: May 22, 2003Date of Patent: February 22, 2011Inventors: Karsten Koenig, Uday Krishna Tirlapur
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Patent number: 7534598Abstract: A surface of a cell is irradiated with laser beam to make an opening in the cell membrane, and a drug fluid is inserted in the cell through the opening.Type: GrantFiled: February 28, 2005Date of Patent: May 19, 2009Assignee: Fujitsu LimitedInventors: Moritoshi Ando, Satoru Sakai
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Patent number: 7524327Abstract: In accordance with the present invention, methods are provided for treating a patient through the use of ultraviolet light activated gene therapy. Embodiments of the present invention include methods for the utilization of light activated gene therapy to repair and/or rebuild damaged cartilage by introducing a desired gene into a patient's tissue.Type: GrantFiled: January 31, 2003Date of Patent: April 28, 2009Assignee: University of RochesterInventors: Edward M. Schwarz, Regis J. O'Keefe, Thomas Foster, Jarod C. Finlay
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Patent number: 7521239Abstract: A method for introducing a molecule into the cytosol of a cell in which the cell is contacted with a photosensitizing agent, the cell is irradiated with light of a wavelength effective to activate the photosensitizing agent and, substantially at the same time or after the irradiation, the cell is contacted with the molecule to be introduced, particularly for use in cancer treatment, gene therapy and vaccination.Type: GrantFiled: November 29, 2001Date of Patent: April 21, 2009Assignee: PCI Biotech ASInventors: Anders Høgset, Kristian Berg, Gunhild Mari Mælandsmo, Birgit Øvstebø Engesæter, Lina Prasmickaite
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Patent number: 7300795Abstract: Optoinjection method for transiently permeabilizing a target cell by (a) illuminating a population of cells contained in a frame; (b) detecting at least one property of light directed from the frame; (c) locating a target cell by the property of light; and (d) irradiating the target cell with a pulse of radiation.Type: GrantFiled: March 30, 2004Date of Patent: November 27, 2007Assignee: Cyntellect, Inc.Inventors: Manfred R. Koller, Elie G. Hanania, Timothy M. Eisfeld, Bernhard Ø. Palsson
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Patent number: 7223600Abstract: The present invention provides a method for introducing a molecule into the cytosol of a cell in which the cell is contacted with a photosentistising agent, the cell is irradiated with light of a wavelength effective to activate the photosentisitising agent and, substantially at the same time or after the irradiation, the cell is contacted with the molecule to be introduced, particularly for use in cancer treatment, gene therapy and vaccination.Type: GrantFiled: November 29, 2001Date of Patent: May 29, 2007Assignee: The Norwegian Radium Hospital Research FoundationInventors: Kristian Berg, Lina Prasmickaite, Anders Høgset, Pål Kristian Selbo
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Patent number: 7151203Abstract: Disclosed are methods for producing grape plants having resistance to bunch rot disease or Botrytis or both. Also disclosed are grape plants having resistance to bunch rot disease or Botrytis or both, wherein the plants express a cecropin B peptide Shiva-1.Type: GrantFiled: May 19, 2003Date of Patent: December 19, 2006Assignees: University of Florida Research Foundation, Inc., The United States of America as represented by the Secretary of AgriculureInventors: Ralph Scorza, Dennis J. Gray
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Patent number: 7132289Abstract: A method for introducing a foreign matter into a cell, includes the steps of placing a small particle carrying a foreign matter at a part of a cell surface of a living cell, boring a hole in a cell wall and/or a cell membrane by irradiating and treating said part of the cell surface with a laser beam, and introducing the foreign matter into the living cell.Type: GrantFiled: December 17, 2001Date of Patent: November 7, 2006Assignee: Osaka UniversityInventors: Akio Kobayashi, Kiichi Fukui, Satoshi Harajima, Eiichiro Fukusaki, Shinichiro Kajiyama, Shinya Okuda, Takeshi Shoji
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Patent number: 6753171Abstract: A technique for controlling membrane denaturation reactions other than physical shear force was developed. For example, the present invention provides, a method for causing membrane disruption at a specific site by reacting a stimulus such as light with a compound that is activated by the stimulus, where the reaction occurs on a membrane such as a biomembrane. It also provides a membrane structure such as cells in which a specific site has been disrupted, which are obtained by the present method. Introduction of substances such as genes also became possible by using this membrane structure. Further provided is a membrane-destroying member for disrupting a membrane at a specific site. Thus, the present invention enabled, for example, easy membrane penetration using components constituting microelectrodes, micromanipulators, and microinjectors, which were conventionally hardly usable in penetrating cell membranes.Type: GrantFiled: December 28, 2000Date of Patent: June 22, 2004Assignee: Center for Advanced Science and Technology Incubation, Ltd.Inventors: Isao Karube, Takashi Saitoh
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Patent number: 6664228Abstract: A drug delivery system and methods are provided wherein a therapeutic/diagnostic agent or a component of the immune system is directed to particular cells in a selected organ or a specific site. Such a system can be formed by a photoselective compound generally described as V-M-P-C. V is a vector component suitable to target particular cells in a selected organ or site, where the type of the cell or antigen does not have to be specific to the desired treatment site. M is a marker component that is capable of being targeted by a therapeutic/diagnostic agent or a component of the immune system. P is a photocleavable or photosensitive bond, and C is a cap component rendering M ineffective. C is connected to M by the photocleavable or photosensitive bond. The photoselective compound is administered and marks targets of a certain kind of cell or a specific site.Type: GrantFiled: August 10, 2000Date of Patent: December 16, 2003Assignee: CeramOptec Industries, Inc.Inventors: Jörg G Moser, Wolfgang Neuberger
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Patent number: 6534308Abstract: This invention provides a method and apparatus for selectively identifying, and targeting with an energy beam, specific cells within a mixed cell population, for the purpose of inducing a response in the targeted cells. Using the present invention, every detectable cell in a population can be identified and affected, without substantially affecting non-targeted cells within the mixture.Type: GrantFiled: November 30, 1999Date of Patent: March 18, 2003Assignee: Oncosis, LLCInventors: Bernhard O. Palsson, Manfred R. Koller, Timothy M. Eisfeld
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Publication number: 20020142465Abstract: A method for processing a cell, including the steps of irradiating a cell or a living tissue with a laser beam through an optical fiber, and cutting off, removing or boring a cell wall and/or a cell membrane or an entirety of the cell thus irradiated.Type: ApplicationFiled: January 10, 2002Publication date: October 3, 2002Applicant: OSAKA UNIVERSITYInventors: Akio Kobayashi, Kiichi Fukui, Satoshi Harajima, Eiichiro Fukusaki, Shinichiro Kajiyama, Shinya Okuda, Takeshi Shoji
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Patent number: 6103959Abstract: An inbred corn line, designated ASG28, is disclosed. The invention relates to the seeds of inbred corn line ASG28, to the plants of inbred corn line ASG28 and to methods for producing a corn plant produced by crossing the inbred line ASG28 with itself or another corn line. The invention further relates to hybrid corn seeds and plants produced by crossing the inbred line ASG28 with another corn line.Type: GrantFiled: December 9, 1998Date of Patent: August 15, 2000Assignee: Monsanto CorporationInventor: Rodney Tietz