Yeast Is A Host For The Plasmid Or Episome Patents (Class 435/483)
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Patent number: 8142793Abstract: Immunogenic compositions that elicit immune responses against Norovirus and Sapovirus antigens are described. In particular, the invention relates to polynucleotides encoding one or more capsid proteins or other immunogenic viral polypeptides from one or more strains of Norovirus and/or Sapovirus, coexpression of such immunogenic viral polypeptides with adjuvants, and methods of using the polynucleotides in applications including immunization and production of immunogenic viral polypeptides and viral-like particles (VLPs). Methods for producing Norovirus- or Sapovirus-derived multiple epitope fusion antigens or polyproteins and immunogenic compositions comprising one or more immunogenic polypeptides, polynucleotides, VLPs, and/or adjuvants are also described.Type: GrantFiled: March 24, 2009Date of Patent: March 27, 2012Assignee: Novartis Vaccines & Diagnostics, Inc.Inventors: Doris Coit, Michael Houghton, Colin McCoin, Angelica Medina-Selby, Michael Vajdy
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Patent number: 8143061Abstract: The invention relates to an industrial method for producing an interest heterologous RNA and to a system for carrying out said production method consisting (1) in transforming mitochondrions of yeast cells free of mitochondrial RNA with a mitochondrial transcription vector comprising at least one copy of the DNA encoding said interest heterologous RNA which are controlled by regulatory element(s) of the mitochondrial transcription and a reporter gene thereof or a fragment of said reporter gene, 2) identifying yeast transformants by incorporating the interest DNA into the mitochondrions thereof, (3) culturing the yeast mitochondrial transformants selected at the stage (2), (4) isolating the mitochondrions from the yeast mitochondrial transformants obtainable at stages (3) and in extracting and purifying the interest heterologous RNA from said mitochondrions.Type: GrantFiled: July 22, 2004Date of Patent: March 27, 2012Assignees: Centre National de la Recherche Scientifique, Universite Victor Segalen Bordeaux 2Inventors: Jean-Paul Di Rago, Nathalie Bonnefoy, Stéphane Duvezin-Caubet
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Patent number: 8137954Abstract: Methods and materials are provided for the production of compositions of erythropoietin protein, wherein said compositions comprise a pre-selected N-linked glycosylation pattern as the predominant N-glycoform.Type: GrantFiled: May 13, 2010Date of Patent: March 20, 2012Assignee: Glycofi, Inc.Inventors: Natarajan Sethuraman, Juergen Nett, Robert Davidson
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Patent number: 8133715Abstract: The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.Type: GrantFiled: March 29, 2011Date of Patent: March 13, 2012Assignee: Gevo, Inc.Inventors: Thomas Buelter, Andrew Hawkins, Stephanie Porter-Scheinman, Peter Meinhold, Catherine Asleson Dundon, Aristos Aristidou, Jun Urano, Matthew Peters, Melissa Dey, Justas Jancauskas, Julie Kelly, Ruth Berry
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Patent number: 8124104Abstract: Immunogenic compositions that elicit immune responses against Norovirus and Sapovirus antigens are described. In particular, the invention relates to polynucleotides encoding one or more capsid proteins or other immunogenic viral polypeptides from one or more strains of Norovirus and/or Sapovirus, coexpression of such immunogenic viral polypeptides with adjuvants, and methods of using the polynucleotides in applications including immunization and production of immunogenic viral polypeptides and viral-like particles (VLPs). Methods for producing Norovirus- or Sapovirus-derived multiple epitope fusion antigens or polyproteins and immunogenic compositions comprising one or more immunogenic polypeptides, polynucleotides, VLPs, and/or adjuvants are also described.Type: GrantFiled: March 24, 2009Date of Patent: February 28, 2012Assignee: Novartis Vaccines & Diagnostics, IncInventors: Doris Coit, Michael Houghton, Colin McCoin, Angelica Medina-Selby, Michael Vajdy
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Patent number: 8119145Abstract: Immunogenic compositions that elicit immune responses against Norovirus and Sapovirus antigens are described. In particular, the invention relates to polynucleotides encoding one or more capsid proteins or other immunogenic viral polypeptides from one or more strains of Norovirus and/or Sapovirus, coexpression of such immunogenic viral polypeptides with adjuvants, and methods of using the polynucleotides in applications including immunization and production of immunogenic viral polypeptides and viral-like particles (VLPs). Methods for producing Norovirus- or Sapovirus-derived multiple epitope fusion antigens or polyproteins and immunogenic compositions comprising one or more immunogenic polypeptides, polynucleotides, VLPs, and/or adjuvants are also described.Type: GrantFiled: March 24, 2009Date of Patent: February 21, 2012Assignee: Novartis Vaccines & Diagnostics, Inc.Inventors: Doris Coit, Michael Houghton, Colin McCoin, Angelica Medina-Selby, Michael Vajdy
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Patent number: 8119146Abstract: HBsAg is expressed in a Saccharomyces cerevisiae host, carrying a plasmid having a HBsAg coding sequence, wherein the plasmid includes: (1) an upstream promoter from a glyceraldehyde-3-phosphate dehydrogenase gene, for controlling expression of the HBsAg coding sequence; and (2) an ARG3 transcription terminator downstream of the HBsAg coding sequence. The plasmids may also include: (3) a LEU2 selection marker; (4) a 2? plasmid sequence; and (5) an origin of replication functional in Escherichia coli. HBsAg can be expressed in this host, and can be purified for use in the manufacture of vaccines, and in particular for the manufacture of combination vaccines and in new monovalent HBV vaccines e.g. with non-alum adjuvants.Type: GrantFiled: April 14, 2006Date of Patent: February 21, 2012Inventor: Angelica Medina-Selby
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Patent number: 8114629Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate keto amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with keto amino acids using these orthogonal pairs.Type: GrantFiled: October 26, 2007Date of Patent: February 14, 2012Assignee: The Scripps Research InstituteInventors: Peter G. Schultz, Lei Wang
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Patent number: 8110360Abstract: The present invention provides compositions and methods for rapid assembly of one or more assembled polynucleotides from a plurality of component polynucleotides. The methods of the invention utilize circular nucleic acid vectors that comprise a DNA segment D flanked by an annealable linker sequence, annealable linker sequence pairs LA and LB, or annealable linker sequence/primer binding segment pairs LA and PB or PA and LB. Restriction endonuclease digestion of a plurality of vectors containing the DNA segments to be assembled generates a plurality of DNA fragments comprising the elements PA-D-LB, LA-D-LB, and LA-D-PB or D-LB, LA-D-LB, and LA-D. The sequences of annealable linker sequences LA and LB provide complementary termini to the DNA fragments, which are utilized in host cell mediated homologous recombination or together with primer binding segments PA and PB in a polymerase cycling assembly reaction for the ordered assembly of the various DNA segments into one or more assembled polynucleotides.Type: GrantFiled: January 8, 2010Date of Patent: February 7, 2012Assignee: Amyris, Inc.Inventors: Zach Serber, Raymond Lowe, Jeffrey A. Ubersax, Sunil S. Chandran
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Patent number: 8110382Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.Type: GrantFiled: August 12, 2008Date of Patent: February 7, 2012Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, Anna Panova, Jeffery Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
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Patent number: 8084238Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.Type: GrantFiled: August 12, 2008Date of Patent: December 27, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, Anna Panova, Jeffery Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
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Patent number: 8071343Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.Type: GrantFiled: August 12, 2008Date of Patent: December 6, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, Anna Panova, Jeffery Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
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Patent number: 8039238Abstract: The present invention provides a transformant into which has been incorporated DNA for coding a foreign protein having lactate dehydrogenase activity and provided with pyruvic acid substrate affinity that equals or exceeds the pyruvic acid substrate affinity of the pyruvate decarboxylase inherent in the host organism. Said transformant can stably mass-produce lactic acid inside a host organism having the pyruvate decarboxylase gene.Type: GrantFiled: March 11, 2003Date of Patent: October 18, 2011Assignee: Toyota Jidosha Kabushiki KaishaInventors: Satoshi Saito, Osamu Saotome, Noriko Yasutani, Yasuo Matsuo, Nobuhiro Ishida, Masana Hirai, Takao Imaeda, Chikara Miyazaki, Kenro Tokuhiro
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Patent number: 8034607Abstract: A method of enhancing heterologous protein secretion in a yeast cell is disclosed. In one embodiment, the method comprising the steps of engineering a yeast cell to overexpress at last one gene selected from the group consisting of CCW12, CWP2, SED1, RPP0, ERO1 and their homologs, supplying the yeast cell with a nucleic acid encoding a heterologous protein and obtaining increased expression of the heterologous protein, wherein the expression is increased relative to the protein expression in a yeast cell that does not overexpress a gene selected from the group consisting of CCW12, CWP2, SED1, RPP0, ERO1 and their homologs.Type: GrantFiled: September 12, 2008Date of Patent: October 11, 2011Assignee: Wisconsin Alumni Research FoundationInventors: Eric V. Shusta, Alane E. Wentz
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Patent number: 8008052Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.Type: GrantFiled: August 12, 2008Date of Patent: August 30, 2011Assignee: E.I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, Anna Panova, Jeffery Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
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Patent number: 7968341Abstract: The present invention relates to a method of sequence-specific recombination of DNA in eukaryotic cells, comprising the introduction of a first DNA comprising a nucleotide sequence containing at least one recombination sequence into a cell, introducing a second DNA comprising a nucleotide sequence containing at least one further recombination sequence into a cell, and performing the sequence specific recombination by a bacteriophage lambda integrase Int.Type: GrantFiled: November 28, 2003Date of Patent: June 28, 2011Assignees: Boehringer Ingelheim Pharma GmbH & Co KGInventors: Peter Dröge, Barbara Enenkel
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Patent number: 7955596Abstract: The present invention pertains to the discovery that B. anthracis possesses a luxS gene that encodes a functional LuxS polypeptide, and that B. anthracis synthesizes a functional AI-2 quorum-sensing molecule. The invention provides mutant B. anthracis bacteria lacking the function of the luxS gene, which do not produce a functional AI-2 molecule and have growth defects compared to wild-type B. anthracis. The invention also concerns methods for inhibiting the growth of B. anthracis, or for preventing or treating B. anthracis infection, by inhibiting the activity of the B. anthracis LuxS polypeptide, or by exposure of the B. anthracis to furanone. In particular, the invention concerns the use of furanone, a compound that inhibits AI-2-mediated quorum-sensing, to inhibit the growth of B. anthracis, to inhibit B. anthracis toxin production, particularly that of protective antigen, and to prevent or treat B. anthracis infection. The invention also provides methods to prevent B.Type: GrantFiled: March 7, 2008Date of Patent: June 7, 2011Assignees: New York University, University of ConnecticutInventors: Marcus B. Jones, Martin J. Blaser, Thomas Wood, Dacheng Ren
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Patent number: 7947874Abstract: The present invention provides methods of modifying in vivo mutagenesis or homeologous recombination in a eukaryote. The method of modifying in vivo mutagenesis involves transforming a eukaryote with a nucleotide sequence capable of expressing a wild-type prokaryotic MutS, MutL, MutH, MutU, NLS-MutS, NLS-MutL, NLS-MutH, NLS-MutU protein, or a combination thereof, and expressing the protein. A method of modifying recombination between homeologous chromosomes in an allopolyploid eukaryotic organism comprising, expressing a nucleotide sequence encoding prokaryotic NLS-MutS in combination with one or more than one of NLS-MutL, NLS-MutH, NLS-MutU, within a germ cell of the allopolyploid eukaryotic organism is also disclosed.Type: GrantFiled: December 6, 2004Date of Patent: May 24, 2011Assignee: Her Majesty the Queen in right of Canada, as represented by the Minister of Agriculture and AgrifoodInventors: Kevin L. Rozwadowski, Derek J. Lydiate
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Patent number: 7939303Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.Type: GrantFiled: August 12, 2008Date of Patent: May 10, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, Anna Panova, Jeffery Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
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Patent number: 7927585Abstract: Stocks of infectious rAAV are generated using yeast strains, bacterial strains, and bacteriophages engineered to express the required AAV proteins and harboring rAAV vector sequences. Stocks of rAAV virions of all serotypes and pseudotypes can be generated in prokaryotic and eukaryotic cells using the methods described herein.Type: GrantFiled: January 25, 2008Date of Patent: April 19, 2011Assignee: University of Florida Research Foundation, Inc.Inventor: Richard O. Snyder
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Patent number: 7923221Abstract: The invention relates to processes for producing an immunoglobulin or an immunologically functional immunoglobulin fragment containing at least the variable domains of the immunoglobulin heavy and light chains. The processes can use one or more vectors which produce both the heavy and light chains or fragments thereof in a single cell. The invention also relates to the vectors used to produce the immunoglobulin or fragment, and to cells transformed with the vectors.Type: GrantFiled: April 13, 1995Date of Patent: April 12, 2011Assignees: Genentech, Inc, City of HopeInventors: Shmuel Cabilly, Herbert L. Heyneker, William E. Holmes, Arthur D. Riggs, Ronald B. Wetzel
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Patent number: 7892786Abstract: The present invention relates to a method of expressing an immunotoxin in Pichia pastoris strain mutated to toxin resistance comprising a) growing the Pichia pastoris in a growth medium comprising an enzymatic digest of protein and yeast extract and maintaining a dissolved oxygen concentration at 40% and above; and b) performing methanol induction with a limited methanol feed of 0.5-0.75 ml/min/IO L of initial volume during induction along with a continuous infusion of yeast extract at a temperature below 17.5° C., antifoaming agent supplied up to 0.07%, agitation reduced to 400 RPM, and the induction phase extended out to 163 h.Type: GrantFiled: August 2, 2004Date of Patent: February 22, 2011Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: David M. Neville, Jung-Hee Woo, Yuan-Yi Liu
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Publication number: 20100304490Abstract: Provided herein are methods of generating genetically modified microorganisms, e.g., genetically modified yeast strains, which comprise functional disruptions in one or more pheromone response genes and one or more sporulation genes, and genetically modified yeast cells, e.g., genetically modified diploid and haploid yeast cells, that lack sporulation capability and endogenous mating capability, produced thereby.Type: ApplicationFiled: June 1, 2010Publication date: December 2, 2010Inventor: Jeffrey A. Ubersax
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Patent number: 7838278Abstract: Nucleotide and protein sequences that encode enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as a R. oryzae cell, are provided. Methods of manipulating carbon flux in a cell also are provided.Type: GrantFiled: March 23, 2009Date of Patent: November 23, 2010Assignee: Archer-Daniels-Midland CompanyInventors: Beth Fatland-Bloom, P. John Rayapati, Nyerhovwo John Tonukari
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Publication number: 20100291648Abstract: The invention relates to global transcription machinery engineering to produce altered cells having improved phenotypes.Type: ApplicationFiled: December 7, 2007Publication date: November 18, 2010Applicants: MASSACHUSETTS INSTITUTE OF TECHNOLOGY, WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCHInventors: Hal S. Alper, Gregory Stephanopoulos, Gerald Fink
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Patent number: 7820423Abstract: The present application provides a method for predicting the functional site of a protein using data of the entire proteins of an organism of which genome data or cDNA data is known. More specifically, the present application provides a method for predicting a protein functional site, comprising the steps of calculating the frequency of occurrence of an oligopeptide in the entire proteins, calculating the value of each amino-acid residue contributing to the frequency of occurrence as the representative value of the function, and predicting the protein functional site by using the representative value of function as an indicator. The present also provides a system for predicting a functional site for automatically performing said methods.Type: GrantFiled: May 31, 2007Date of Patent: October 26, 2010Assignee: Japan Science And Technology CorporationInventors: Hirofumi Doi, Hideaki Hiraki, Akio Kanai
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Patent number: 7759121Abstract: Yeast cells are mutagenized to obtain desirable mutants. Mutagenesis is mediated by a defective mismatch repair system which can be enhanced using conventional exogenously applied mutagens. Yeast cells with the defective mismatch repair system are hypermutable, but after selection of desired mutant yeast strains, they can be rendered genetically stable by restoring the mismatch repair system to proper functionality.Type: GrantFiled: October 31, 2007Date of Patent: July 20, 2010Assignees: The John Hopkins University, Morphotek, Inc.Inventors: Nicholas C. Nicolaides, Philip M. Sass, Luigi Grasso, Bert Vogelstein, Kenneth W. Kinzler
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Patent number: 7741083Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.Type: GrantFiled: August 12, 2008Date of Patent: June 22, 2010Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, Anna Panova, Jeffery Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
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Patent number: 7732172Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.Type: GrantFiled: August 12, 2008Date of Patent: June 8, 2010Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, Anna Panova, Jeffrey Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
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Patent number: 7713719Abstract: The present invention provides methods to reduce or eliminate ?-mannosidase resistant glycans on glycoproteins in yeast. The reduction or elimination of ?-mannosidase resistant glycans on glycoproteins results from the disruption of the newly isolated P. pastoris AMR2 gene encoding ?1,2-mannosyltransferase. The present invention also discloses novel genes, polypeptides, antibodies, vectors and host cells relating to ?-mannosidase resistance on glycans.Type: GrantFiled: November 7, 2008Date of Patent: May 11, 2010Assignee: GlycoFi, Inc.Inventor: Piotr Bobrowicz
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Patent number: 7666678Abstract: The present invention provides genes and proteins having possibilities to generate plants having tolerance against excessive boron, which can confer a boric acid tolerance to organisms. 5 types of genes that can confer a boric acid tolerance to yeast, such as AtPAB2, AtRBP47c?, AtRPS20B, AtMYB13 and AtMYB68, AtRBP45a, AtRBP45b, AtRBP45c, AtRBP45d, AtRBP47a, AtRBP47b, AtRBP47c, AtUBP1a, AtUBP1b and AtUBP1c which were found by expressing several genes of higher plant Arabidopsis thaliana in yeast that is a organism model of eukaryote. Further, a key to the toxicity mechanism of boric acid exists in the specific inhibition of splicing, and a gene related to enhancement of splicing efficiency also confers a boric acid tolerance.Type: GrantFiled: September 15, 2006Date of Patent: February 23, 2010Assignee: Japan Science and Technology AgencyInventors: Toru Fujiwara, Akira Nozawa
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Patent number: 7622275Abstract: A yeast cell containing constitutively expressed aequorin and methods of using the cells in growth and toxicity assays are disclosed.Type: GrantFiled: December 14, 2004Date of Patent: November 24, 2009Assignee: Sanofi-Aventis Deutschland GmbHInventors: Pauline Fraissignes, Denis Guedin
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Patent number: 7618793Abstract: Methods of screening candidate agents to identify potential therapeutic agents for the treatment of a neurodegenerative disease, such as Huntington's Disease and Parkinson's Disease and methods for identifying a mutation in, or changes in expression of, a gene associated with neurodegenerative disease, such as Huntington's Disease and Parkinson's Disease, are provided.Type: GrantFiled: October 20, 2004Date of Patent: November 17, 2009Assignee: The Regents of the University of WashingtonInventors: Paul J. Muchowski, Flaviano Giorgini
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Patent number: 7612177Abstract: A method is presented for selecting and isolating nucleic acids capable of conferring tolerance or resistance to environmental stress conditions in plants or yeast. Furthermore, nucleic acids, the proteins they encode and their use for the production of plants or yeast with enhanced environmental stress resistance is disclosed.Type: GrantFiled: April 13, 2004Date of Patent: November 3, 2009Assignee: Cropdesign N.V.Inventors: Jose Miguel Mulet Salort, Ramon Serrano Salom
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Patent number: 7566563Abstract: Nucleotide sequences and genetic constructs that can be used to regulate genes encoding enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as a R. oryzae cell, are provided. Methods of manipulating carbon flux in a cell also are provided.Type: GrantFiled: August 21, 2008Date of Patent: July 28, 2009Assignee: Archer-Daniels-Midland CompanyInventors: Beth Fatland-Bloom, Gyan Rai, P. John Rayapati, Nyerhovwo John Tonukari
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Publication number: 20090136924Abstract: Methods are described for construction of long synthetic arrays of DNA repeats, such as alphoid repeats or other repeat sequences. The methods include concatamerization of DNA into short repeats (for instance using rolling circle amplification or directional in vitro ligation), followed by assembling the short repeats into long arrays by homologous recombination during transformation into microbe cells. These methods can be described generally as Recombinational Amplification of Repeats (RAR). The long arrays are engineered centromere-like regions that allow one to construct mammalian artificial chromosomes with a predefined centromeric region structure.Type: ApplicationFiled: April 8, 2006Publication date: May 28, 2009Inventors: Vladimir L. Larionov, William C. Earnshaw, Reto Gassman, Stefanie Kandels-Lewis, Hiroshi Masumoto, Megumi Nakano, Stefano Cardinale, Carl J Barrett, Vladimir Noskov, Natalay Y. Kouprina
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Patent number: 7527801Abstract: Immunogenic compositions that elicit immune responses against Norovirus and Sapovirus antigens are described. In particular, the invention relates to polynucleotides encoding one or more capsid proteins or other immunogenic viral polypeptides from one or more strains of Norovirus and/or Sapovirus, coexpression of such immunogenic viral polypeptides with adjuvants, and methods of using the polynucleotides in applications including immunization and production of immunogenic viral polypeptides and viral-like particles (VLPs). Methods for producing Norovirus- or Sapovirus-derived multiple epitope fusion antigens or polyproteins and immunogenic compositions comprising one or more immunogenic polypeptides, polynucleotides, VLPs, and/or adjuvants are also described.Type: GrantFiled: November 22, 2006Date of Patent: May 5, 2009Assignee: Novartis Vaccines and Diagnostics, Inc.Inventors: Doris Coit, Michael Houghton, Colin McCoin, Angelica Medina-Selby, Michael Vajdy
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Patent number: 7524647Abstract: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate keto amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with keto amino acids using these orthogonal pairs.Type: GrantFiled: October 26, 2007Date of Patent: April 28, 2009Assignee: The Scripps Research InstituteInventors: Peter G. Schultz, Lei Wang
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Patent number: 7514216Abstract: Yeast cells are mutagenized to obtain desirable mutants. Mutagenesis is mediated by a defective mismatch repair system which can be enhanced using conventional exogenously applied mutagens. Yeast cells with the defective mismatch repair system are hypermutable, but after selection of desired mutant yeast strains, they can be rendered genetically stable by restoring the mismatch repair system to proper functionality.Type: GrantFiled: July 26, 2005Date of Patent: April 7, 2009Assignee: The Johns Hopkins UniversityInventors: Nicholas C. Nicolaides, Philip M. Sass, Luigi Grasso, Bert Vogelstein, Kenneth W. Kinzler
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Patent number: 7507574Abstract: The present invention provides a modified Saccharomyces yeast which produces significantly lower levels of ethanol than wild-type yeast under aerobic conditions and saccharide concentrations of 2% glucose, and which exhibits a growth rate of at least 30% of the wild-type yeast, preferably containing a chimeric construct of at least 2 saccharide transporters, nucleic acid molecules encoding the chimeras and polypeptides encoded by such sequences, and methods of using the modified yeast for preparing products in the yeast.Type: GrantFiled: June 29, 2001Date of Patent: March 24, 2009Assignee: Gothia Yeast Solutions ABInventors: Roslyn Bill, Eckhard Boles, Lena Gustafsson, Stefan Hohmann, Christer Larsson, Karin Elbing
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Patent number: 7501268Abstract: A method of producing a prenyl alcohol, comprising creating a recombinant by transferring into a host a recombinant DNA for expression or a DNA for genomic integration each comprising a prenyl diphosphate synthase gene or a mutant thereof, culturing the resultant recombinant, and recovering the prenyl alcohol from the resultant culture.Type: GrantFiled: December 20, 2001Date of Patent: March 10, 2009Assignee: Toyota Jidosha Kabushiki KaishaInventors: Chikara Ohto, Shusei Obata, Masayoshi Muramatsu, Kiyohiko Nishi, Kazuhiko Totsuka
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Publication number: 20090035755Abstract: The present invention provides screening procedures for identifying inhibitors of components of regulatory networks by a positive phenotype and modified yeast cell lines suitable for said screening. The screening procedures are especially suited to screen for substances that re-sensitize resistant pathogenic microorganisms or tumor cells by suspending the expression of resistance-relevant genes. The invention further provides methods for constructing said cell lines and their use in screening systems.Type: ApplicationFiled: November 25, 2005Publication date: February 5, 2009Applicant: University of South BohemiaInventors: Milan Höfer, Jost Ludwig, Petra Schwanewilm, Juilus Subik
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Patent number: 7479389Abstract: Novel genes encoding P. pastoris ARG1, ARG2, ARG3, HIS1, HIS2, HIS5 and HIS6 are disclosed. A method for inactivating alternately at least two biosynthetic pathways in a methylotrophic yeast is provided. A method for producing and selecting yeast strains characterized as being capable of genetic integration of heterologous sequences into the host genome using the genes involved in the biosynthetic pathways is also disclosed.Type: GrantFiled: March 2, 2005Date of Patent: January 20, 2009Assignee: GlycoFi, Inc.Inventors: Juergen Nett, Tillman Gerngross
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Patent number: 7462708Abstract: The present invention relates to novel temperature promoters and set of expression vectors isolated from Schizosaccharomyces pombe. The vectors so developed can be used for regulated expression of proteins, both homologous and heterologous, very efficiently and economically.Type: GrantFiled: March 31, 2004Date of Patent: December 9, 2008Assignee: Council of Scientific & Industrial ResearchInventors: Jagmohan Singh, Raj Kumar
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Patent number: 7456020Abstract: The present invention provides a simple method for splitting and loss of a chromosome in yeast. The method for modifying a chromosome in yeast includes preparing a linear chromosome splitting vector (1) having a target sequence (a), a marker gene sequence and (C4A2)n sequence in this order; preparing a linear chromosome splitting vector (2) having a target sequence (b), a centromere sequence of a yeast chromosome and (C4A2)n sequence in this order; and introducing the chromosome splitting vectors (1) and (2) into yeast. Herein, n is each independently an integer of 6 to 10. Although this chromosome splitting vector has a repetitive sequence of 5?-CCCCAA-3?, it can be amplified specifically with PCR, so that a chromosome splitting vector can be prepared significantly simply and easily, compared with the conventional DNA splitting method.Type: GrantFiled: September 11, 2003Date of Patent: November 25, 2008Assignee: Osaka UniversityInventors: Satoshi Harashima, Yoshinobu Kaneko, Minetaka Sugiyama
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Patent number: 7445917Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.Type: GrantFiled: December 21, 2005Date of Patent: November 4, 2008Assignee: E.I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, Anna Panova, Jeffery Scott Thompson, Robert D. Fallon, F. Glenn Gallagher, Thomas Foo, Xu Li, George C. Fox, Joseph J. Zaher, Mark S. Payne, Daniel P. O'Keefe
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Patent number: 7445775Abstract: Vectors for the expression in yeast of mammalian plasminogen derivatives such as microplasminogen and miniplasminogen are presented. Methods for expression of these proteins in a methylotrophic yeast expression system are disclosed as well as the activation and stabilisation of the recombinant proteins. The proteins of this invention are used In the treatment of focal cerebral ischemic infarction and other thrombotic diseases.Type: GrantFiled: December 20, 2001Date of Patent: November 4, 2008Assignee: Thromb-X nvInventors: Désiré José Collen, Nubuo Nagai, Yves Laroche
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Patent number: 7435168Abstract: Nucleotide sequences and genetic constructs that can be used to regulate genes encoding enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as a R. oryzae cell, are provided. Methods of manipulating carbon flux in a cell also are provided.Type: GrantFiled: January 17, 2006Date of Patent: October 14, 2008Assignee: Archer-Daniels-Midland CompanyInventors: Beth Fatland-Bloom, Gyan Rai, P. John Rayapati, Nyerhovwo John Tonukari
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Patent number: 7429469Abstract: This invention relates to a method of producing polyesters resulting from homopolymerization or copolymerization of a 3-hydroxyalkanoic acid(s) and having biodegradability and good physical properties using yeasts as hosts. By constructing at least one enzyme gene involved in polyester synthesis by adding a DNA coding a peroxisome-targeting signal, introducing an enzyme gene expression cassette containing that gene into yeast, and cultivating the thus-obtained transformant, it becomes possible to cause accumulation of a polyester resulting from homopolymerization or copolymerization of a 3-hydroxyalkanoic acid(s) in yeast cells and recover the polyester from the culture.Type: GrantFiled: October 9, 2002Date of Patent: September 30, 2008Assignee: Kaneka CorporationInventors: Satoru Yokomizo, Takeshi Fukuchi, Fumio Osakada, Keiji Matsumoto, Masamichi Takagi, Akinori Ohta
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Patent number: 7425450Abstract: The present invention generally relates to hyphal growth in fungi and in particular describes the modulation of genes associated with hyphal growth in filamentous fungi. The present invention provides methods and systems for the production of proteins and/or chemicals from filamentous fungi which comprise modulation of genes associated with hyphal growth. Specifically, the present invention is directed to a full length cotA gene, its gene product and methods of use.Type: GrantFiled: April 25, 2005Date of Patent: September 16, 2008Assignee: Genencor International, Inc.Inventors: Ali R. Akin, Elizabeth A. Bodie, Shirley M. Burrow, Nigel Dunn-Coleman, Geoffrey Turner, Michael Ward