Bacillus Circulans Patents (Class 435/835)
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Patent number: 6329011Abstract: An antimicrobial agent with a high degree of safety is provided, which is derived from a natural product and can exhibit growth-inhibitory activity against acid-resistant and heat-resistant bacteria such as Alicyclobacillus acidoterrestris, which is resistant against pasteurization and causes spoilage of fruit juice. The antimicrobial agent against acid-resistant and heat-resistant bacteria contains as an effective ingredient alpha-type thionin and/or beta-type thionin. A preservative for fruit juice is also provided, which contains as an effective ingredient the alpha-type thionin and/or beta-type thionin.Type: GrantFiled: October 5, 2000Date of Patent: December 11, 2001Assignees: Director General of Shikoku National Agricultural Experiment Station, Ministry of Agriculture, Forestry and FisheriesInventor: Shigeru Oita
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Patent number: 6165740Abstract: To reduce the effects of the contaminants in the measurement of microorganisms and the reduction of the time necessary for the measurement. Measurement is performed of the microorganism prior to and following culture, and the difference between the two is found. This prevents errors caused by the effect of contaminants contained in the specimens. Since the measurement of the microorganism is performed by means of a flow cytometer, the microorganisms can be measured even when the culture period is short. Moreover, the measurements are accurate, since the contaminants are not measured. Furthermore, the growth form of the microorganisms can be determined by measuring the changes in the intensity of the light emission over the duration of emission of the forward scattered light detected by means of a flow cytometer.Type: GrantFiled: September 29, 1999Date of Patent: December 26, 2000Assignee: Sysmex CorporationInventors: Masakazu Fukuda, Junya Inoue, Akito Terai, Kazuyuki Kanai, Kurayoshi Iseki, Mayumi Kamo
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Patent number: 5888802Abstract: The invention relates to a thermostable xylanase selected from xylanase XP1 having a molecular weight of about 22,500, an isoelectric point at around 8.1 and an optimum temperature for reaction of 70.degree. C. or xylanase XP2 having a molecular weight of about 32,000, an isoelectric point at around 8.5 and an optimum temperature for reaction of 80.degree. C., a gene encoding for the thermostable xylanase, a method for producing the xylanase, applications of the xylanase, a bleaching agent containing the xylanase as an active ingredient, a method for bleaching pulp by using the bleaching agent and Bacillus sp. 2113 and Bacillus sp. 208 both having an ability to produce a thermostable xylanase.Type: GrantFiled: November 3, 1997Date of Patent: March 30, 1999Assignee: New Oji Paper Company Ltd.Inventors: Nobuyuki Fukunaga, Yuji Iwasaki, Satoko Kono, Yukio Kita, Yoshiya Izumi
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Patent number: 5837482Abstract: A medium for detecting staphylococci is described. The medium contains components selective for growing staphylococci, and a glucopyranoside indicator substance in sufficient quantity to distinguish colonies containing Bacillus and other microorganisms from colonies containing staphylococci. Methods of detecting staphylococci utilizing such medium are also described.Type: GrantFiled: January 23, 1997Date of Patent: November 17, 1998Assignee: Minnesota Mining and Manufacturing CompanyInventors: Patrick A. Mach, Marlys E. Lund
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Patent number: 5770393Abstract: A bacteria impermeable container or ampule (10) contains a liquid growth medium and a substrate-indicator complex. The complex includes a substrate component, e.g., starch, and an indicator molecule, e.g., a dye, a fluorescent molecule, or the like, which are tightly bound and complexed, but which are cleavable by a preselected enzyme. A sterilant passes over a carrier (20) for microorganisms which, upon germination, are capable of rapidly generating large quantities of the preselected enzyme. Following the sterilization process, the carrier is immersed in the liquid growth medium. Any viable surviving microorganisms grow, generating the preselected enzyme. The enzymes cleave the bound indicator molecule from the substrate, resulting in a measurable property change in a couple of hours. Typical property changes include fluorescence, a color change, a change in pH which triggers a pH indicator color change, and the like.Type: GrantFiled: April 1, 1997Date of Patent: June 23, 1998Assignee: Steris CorporationInventors: Joseph P. Dalmasso, David A. Freeman
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Patent number: 5744516Abstract: A biodegradable resin molded article molded from a kneaded material obtained by kneading, melting or mixing a biodegradable resin raw material and at least one of a biodegradable additive and an additive made of a substance existing in the nature, an injection molded article of a biodegradable resin containing a biodegradable resin and an anti-biotic substance, a molded article of a resin composition including a polymer material having an ester bond in the polymer main chain thereof and an alkali or acid component in an amount effective for neutralizing an acidic or alkaline component contained in the polymer material, and a resin molded article having a layer of a biodegradable resin, and a layer of a photolytic resin covering the resin layer and containing an antibiotic.Type: GrantFiled: September 11, 1996Date of Patent: April 28, 1998Assignee: Fujitsu LimitedInventors: Takafumi Hashitani, Shozo Fujita, Makoto Iijima, Koji Asano
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Patent number: 5551515Abstract: Soil bacteria can be isolated which produce an enzyme capable of catalyzing the degradation of mannan-containing hemicellulose under conditions combining high pH and high temperature. Such bacteria can be cultured or used as sources of genetic information with which to engineer other microorganisms to produce the enzyme. Commercially useful quantities of native or recombinant hemicellulase can thus be produced by cultures consisting essentially of microorganisms capable of producing the enzyme.Type: GrantFiled: May 26, 1995Date of Patent: September 3, 1996Assignee: Chemgen CorporationInventors: Douglas W. Fodge, David M. Anderson, Thomas M. Pettey
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Patent number: 5498526Abstract: Biological indicators are provided for use in validating and routinely monitoring oxidizing gas sterilizations. The biological indicators are based on Bacillus circulans spores that are enclosed in packages with sufficient permeability to admit a sterilizing amount of vapor while being substantially bacteria impermeable.Type: GrantFiled: August 25, 1993Date of Patent: March 12, 1996Assignee: Abtox, Inc.Inventors: Ross A. Caputo, Phillip A. Martens
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Patent number: 5405759Abstract: Disclosed are novel enzymes, heparitinase T-I, heparitinase T-II, heparitinase T-III and heparitinase T-IV, which degrade heparan sulfate and/or heparin, a process for producing thereof by cultivating a novel Bacillus circulans HpT 298 having an ability of producing these enzymes and a novel Bacillus circulans HpT 298.Type: GrantFiled: November 18, 1993Date of Patent: April 11, 1995Assignee: Seikagaku Kogyo Kabushiki KaishaInventors: Kiyoshi Morikawa, Hirofumi Miyazono, Hiroshi Maruyama, Keiichi Yoshida
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Patent number: 5290695Abstract: Disclosed are novel enzymes, heparitinase T-I, heparitinase T-II, heparitinase T-III and heparitinase T-IV, which degrade heparan sulfate and/or heparin, a process for producing thereof by cultivating a novel Bacillus circulans HpT 298 having an ability of producing these enzymes and a novel Bacillus circulans HpT 298.Type: GrantFiled: February 28, 1992Date of Patent: March 1, 1994Assignee: Seikagaku Kogyo Kabushiki KaishaInventors: Kiyoshi Morikawa, Hirofumi Miyazono, Hiroshi Maruyama, Keiichi Yoshida
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Patent number: 5061490Abstract: A biological inoculant is disclosed for facilitating and fostering the growth of edible corn plants. The inoculant includes biologically pure cultures of bacterial strains, including Bacillus circulans, a yet unidentified bacterial strain, and Xanthomonas maltotphilia.Type: GrantFiled: May 5, 1990Date of Patent: October 29, 1991Assignee: W. R. Grace & Co.-Conn.Inventors: Alan S. Paau, Dennis E. McCabe, Steven G. Platt
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Patent number: 5053328Abstract: The preparation of an L-amino acid, particularly valine, leucine, isoleucine, alanine and phenylalanine, from the corresponding .alpha.-keto carboxylic acid by bacterial fermentation in the presence of ammonium ions is carried out with the aid of thermophilic Bacillus strains at temperatures above 45.degree. C., in particular above 60.degree. C. Bacillus strains DSM 406, 452, 461, 42, 463, 465 and 466 are particularly suitable for this purpose. The greater solubility of the amino acid at the elevated fermentation temperature permits the separation out of the amino acid from the reaction mixture simply by cooling, whereafter the depleted reaction mixture can be pumped back into the fermenter. Especially favorable yields are achieved by supplying oxygen to the fermenter to an amount of less than about 20% dissolved oxygen.Type: GrantFiled: September 18, 1986Date of Patent: October 1, 1991Assignees: Kernforschungsanlage Juelich, Degussa AGInventors: Bernhard Muetze, Christian Wandrey, Wolfgang Leuchtenberger, Toshihisa Ohshima
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Patent number: 4958016Abstract: Bifunctional oligosaccharides, characterized by the formula ##STR1## wherein R.sub.1 represents a hydrogen atom, an alkyl group having 1-4 carbon atoms or an alkali metal, R.sub.2 represents a single bond or a divalent radical compound of monosaccharides and glc represents glucose, are obtained with the help of a specific microbial lyase cleaving the glucose-glucoronic acid bond in polysaccharides; said bifunctional oligosaccharides can be reacted with all kinds of products like proteins and drugs resulting in products having modified properties like solubility etc.Type: GrantFiled: June 3, 1988Date of Patent: September 18, 1990Assignee: Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek TnoInventors: Antonius Kerkenaar, Diederik J. M. Schmedding, Ronald T. M. Van Den Dool
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Patent number: 4929445Abstract: A method for inhibiting Listeria monocytogenes in a food or other material which can be contaminated with this pathogen using a bacteriocin produced by DNA in Pediococcus acidilactici is described. The bacteriocin is particularly produced by Pediococcus acidilactici containing a 6.2 Mdal (9.4 Kilobase pairs) plasmid encoding for the bacteriocin.Type: GrantFiled: January 25, 1988Date of Patent: May 29, 1990Assignee: Microlife Technics, Inc.Inventors: Peter A. Vandenbergh, Michael J. Pucci, Blair S. Kunka, Ebenezer R. Vedamuthu
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Patent number: 4925795Abstract: Maltotetraose or maltose is specifically produced in a high yield from starch by causing an amylase produced by a microorganism of genus Bacillus circulans G-4 to act on the starch.Type: GrantFiled: December 29, 1986Date of Patent: May 15, 1990Assignees: Agency of Industrial Science & Technology, Ministry of International Trade & IndustryInventor: Yoshiyuki Takasaki
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Patent number: 4835262Abstract: A process for preparing pectin which comprises subjecting a plant tissue containing pectic substances to the action of a microorganism which belongs to the genus Bacillus and possesses an activity liberating pectin from a plant tissue but substantially does not possess an activity of decomposing pectin, or a culture broth or processed material thereof to liberate pectin from said plant tissue and recovering the pectin, which allows to obtain readily a pectin of high molecular weight in high yield.Type: GrantFiled: February 26, 1988Date of Patent: May 30, 1989Inventor: Takuo Sakai
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Patent number: 4769329Abstract: A process for the preparation of optically pure D- or L-lactic acid by fermentation of an aqueous nutrient medium, which contains nitrogen, vitamins, aminoacids, sugars and trace elements, by means of a microorganism, at pH 4-6, wherein the nutrient medium contains brewers' yeast as the source of nitrogen, vitamins, aminoacids and trace elements.Type: GrantFiled: June 30, 1982Date of Patent: September 6, 1988Assignee: BASF AktiengesellschaftInventors: Bryan Cooper, Werner Kuesters, Christoph Martin, Hardo Siegel
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Patent number: 4704360Abstract: Methods and compositions are provided for the production and use of enzymes that degrade lipopolysaccharide bioemulsifiers, and, in particular, emulsans. The enzymes may be used to demulsify bioemulsifier-stabilized hydrocarbon-in-water emulsions.Type: GrantFiled: November 30, 1983Date of Patent: November 3, 1987Assignee: Petroleum FermentationsInventors: Yuval Shoham, Eugene Rosenberg, David L. Gutnick
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Patent number: 4396579Abstract: A luminescence detection device for quantitatively detecting an analyte in a liquid sample, which device comprises (1) a reaction compartment having, along a primary axis, opposite end portions, a first of which is for introduction of fluid reagents and sample into the compartment and the other of which forms a light transmissive aperture of predetermined size, the compartment being suitable to hold a composition which luminesces in response to contact with analyte-containing sample; (2) closure means in said first end portion for admitting a cannula, whereby fluid is introduced into said compartment, and for closing the reaction compartment; (3) a photoresponsive imaging layer; (4) means for associating the photoresponsive imaging layer and the reaction compartment such that the photoresponsive imaging layer is positioned preferably substantially perpendicular to the primary axis of the reaction compartment, at a predetermined distance from the end portion forming the aperture so as to be exposed to light emType: GrantFiled: August 6, 1981Date of Patent: August 2, 1983Assignee: Miles Laboratories, Inc.Inventors: Hartmut R. Schroeder, Paul O. Vogelhut
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Patent number: 4360593Abstract: A novel peptide antibiotic complex designated herein as Bu-2470 is produced by fermentation of Bacillus circulans strain G493-B6 (ATCC 31,805). Complex Bu-2470 may be separated into four bioactive peptide antibiotics designated as Bu-2470A, B.sub.1, B.sub.2a, and B.sub.2b. The complex and individual bioactive factors possess significant antimicrobial activity.Type: GrantFiled: February 8, 1982Date of Patent: November 23, 1982Assignee: Bristol-Myers CompanyInventors: Masataka Konishi, Takeo Miyaki, Hiroshi Tsukiura, Hiroshi Kawaguchi
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Patent number: 4350770Abstract: A process for removing oleaginous materials containing those of animal origin from wastewater comprising treating wastewater containing oleaginous material with a microbial combination of:(a) a microorganism of the strain Pseudomonas aeruginosa mutant SGRR.sub.2 ; and(b) at least one of:(i) a microorganism of the genus Bacillus; and(ii) a microorganism of the genus Pseudomonas other than the strain Pseudomonas aeruginosa mutant SGRR.sub.2 ;and the microbial combination of:(a) a microorganism of the strain Pseudomonas aeruginosa mutant SGRR.sub.2 ; and(b) at least one of:(i) a microorganism of the genus Bacillus; and(ii) a microorganism of the genus Pseudomonas other than the strain Pseudomonas aeruginosa mutant SGRR.sub.2.Type: GrantFiled: May 7, 1981Date of Patent: September 21, 1982Assignee: Sybron CorporationInventor: Philip W. Spraker
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Patent number: 4288545Abstract: A process for removing oleaginous materials containing those of animal origin from wastewater comprising treating wastewater containing oleaginous material with a microbial combination of:(a) a microorganism of the strain Pseudomonas aeruginosa mutant SGRR.sub.2 ; and(b) at least one of:(i) a microorganism of the genus Bacillus; and(ii) a microorganism of the genus Pseudomonas other than the strain Pseudomonas aeruginosa mutant SGRR.sub.2 ; and the microbial combination of:(a) a microorganism of the strain Pseudomonas aeruginosa mutant SGRR.sub.2 ; and(b) at least one of:(i) a microorganism of the genus Bacillus; and(ii) a microorganism of the genus Pseudomonas other than the strain Pseudomonas aeruginosa mutant SGRR.sub.2.Type: GrantFiled: January 17, 1979Date of Patent: September 8, 1981Assignee: Sybron CorporationInventor: Philip W. Spraker
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Patent number: 4254227Abstract: The present invention relates to processes for producing syrups or syrup solids containing fructose-terminated oligosaccharides, characterized by subjecting a mixture containing liquefied starch and either fructose or sucrose to the action of immobilized cyclodextrin glucanotransferase E.C. 2.4.1.19.Type: GrantFiled: March 8, 1979Date of Patent: March 3, 1981Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Shigetaka Okada, Sumio Kitahata, Shigeharu Yoshikawa, Kentaro Miyake
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Patent number: 4237230Abstract: A novel lactase having a molecular weight of about 3.times.10.sup.5, an optimum pH value of about 6.0, an optimum temperature of about 60.degree. C. and at least 1 of the ratio of the activity for hydrolyzing lactose to the activity for hydrolyzing a synthetic substrate: o-nitrophenyl-.beta.-D-galactopyranoside (Lact/ONPG ratio), which is produced by cultivating a microorganism of the genus Bacillus being capable of producing the enzyme, particularly Bacillus circulans LOB 377 (ATCC No. 31382) and isolating from the culture broth. This novel lactase is characteristic in the excellent thermostability and the high Lact/ONPG ratio and hence is useful for treating milk and milk products as well as for preventing diarrhea due to lactose intolerance, especially in babies and infants.Type: GrantFiled: June 26, 1979Date of Patent: December 2, 1980Assignee: Daiwa Kasei Kabushiki KaishaInventors: Takao Iida, Sho Ozaki, Toshihiko Kotaka