Streptomyces Griseus Patents (Class 435/897)
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Publication number: 20030031683Abstract: Attenuated immunogenic bacteria having an RpoS+ phenotype, in particular, Salmonella enterica serotype Typhi having an RpoS+ phenotype and methods therefor are disclosed. The Salmonella have in addition to an RpoS+ phenotype, an inactivating mutation in one or more genes which render the microbe attenuated, and a recombinant gene capable of expressing a desired protein. The Salmonella are attenuated and have high immunogenicity so that they can be used in vaccines and as delivery vehicles for genes and gene products. Also disclosed are methods for preparing the vaccine delivery vehicles.Type: ApplicationFiled: May 3, 2002Publication date: February 13, 2003Inventors: Roy Curtiss, Cheryl A. Nickerson
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Amino alcohol dehydrogenase converts keto alcohol to amino alcohol and amino alcohol to keto alcohol
Patent number: 6432688Abstract: A purified amino alcohol dehydrogenase which reductively converts a keto alcohol into an amino alcohol and oxidatively converts an amino alcohol into a keto alcohol is disclosed. The enzyme is NAD(H) dependent, has a molecular weight of 100,000 Da when determined by gel filtration, has a optimum temperature of about 30° C. in reductive amination, an optimum pH of 10.0 in an oxidative deamination and of 7.0 in a reductive amination and can be isolated from Steptomyces virginiae.Type: GrantFiled: January 18, 2000Date of Patent: August 13, 2002Assignee: Daicel Chemical Industries, Ltd.Inventors: Nobuya Ito, Akinobu Matsuyama, Yoshinori Kobayashi -
Patent number: 6383496Abstract: Attenuated immunogenic bacteria having an RpoS+ phenotype, in particular, Salmonella enterica serotype Typhi having an RpoS+ phenotype and methods therefor are disclosed. The Salmonella have in addition to an RpoS+ phenotype, an inactivating mutation in one or more genes which render the microbe attenuated, and a recombinant gene capable of expressing a desired protein. The Salmonella are attenuated and have high immunogenicity so that they can be used in vaccines and as delivery vehicles for genes and gene products. Also disclosed are methods for preparing the vaccine delivery vehicles.Type: GrantFiled: May 18, 1999Date of Patent: May 7, 2002Assignee: Washington UniversityInventors: Roy Curtiss, III, Cheryl A. Nickerson
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Patent number: 5770392Abstract: Materials can be assayed for activity as an inhibitor of post-translational protein phosphorylation by adding the material to a growing culture of a prokaryotic organism such as a streptomycete; allowing the culture to grow for a period of time in the presence of the material; and observing the culture for altered development relative to development of the prokaryotic organism grown in the absence of the material. Observation of altered development is indicative that the material has activity as an inhibitor of post-translational protein phosphorylation. In particular, the material to be tested can be added to a growing culture of the prokaryotic organism by placing a carrier disk bearing the material on a freshly seeded plate. Inhibition of the development of aerial mycelia and spore formation is an indicator that the material has activity as an inhibitor of post-translational protein phosphorylation.Type: GrantFiled: October 17, 1996Date of Patent: June 23, 1998Assignee: TerraGen Diversity Inc.Inventors: Julian E. Davies, Barbara Waters
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Patent number: 5674714Abstract: A process for inducing cytochrome P-450 enzyme production in bacteria of the genus Streptomyces using inducers such as soybean flour, genistein or genistin is described. Uses for the cytochrome P-450 enzymes produced are also discussed as is a process for using genetically engineered Streptomyces to determine the mutagenicity of chemicals.Type: GrantFiled: November 13, 1992Date of Patent: October 7, 1997Assignee: E. I. Du Pont de Nemours and CompanyInventors: Daniel Anthony Kunz, Sima Fateme Sariaslani
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Patent number: 5213971Abstract: A process for inducing cytochrome P-450 enzyme production in bacteria of the genus Streptomyces using inducers such as soybean flour, genistein or genistin is described. Uses for the cytochrome P-450 enzymes produced are also discussed as is a process for using genetically engineered Streptomyces to determine the mutagenicity of chemicals.Type: GrantFiled: August 28, 1991Date of Patent: May 25, 1993Assignee: E. I. Du Pont de Nemours and CompanyInventors: Daniel A. Kunz, Fateme S. Sariaslani
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Patent number: 5169755Abstract: A process for inducting cytochrome P-450 enzyme production in bacteria of the genus Streptomyces using inducers such as soybean flour, genistein or genistin is described. Uses for the cytochrome P-450 enzymes produced are also discussed as is a process for using genetically engineered Streptomyces to determine the mutagenicity of chemicals.Type: GrantFiled: June 16, 1988Date of Patent: December 8, 1992Assignee: E. I. Du Pont de Nemours and CompanyInventors: Fateme S. Sariaslani, Steven E. Buchholz, Charles A. Omer, Paul V. Viitanen
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Patent number: 5134069Abstract: The type III restriction endonuclease SgrAI has the following recognition sequence: ##STR1## cleaves DNA at the cleavage site indicated by the arrows, and is preferably obtainable from microorganisms of the genus Streptomyces. It can be used to recognize and cleave the double-stranded DNA sequence ##STR2## and its complementary sequence.Type: GrantFiled: November 16, 1990Date of Patent: July 28, 1992Assignee: Boehringer Mannheim GmbHInventors: Klaus Kaluza, Bruno Frey, Gudrun Schmitz-Agheguian, Michael Jarsch, Christoph Kessler
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Patent number: 5070015Abstract: Incubation of 13-deoxy ivermectin aglycone with a species of B. subtilis and of S. griseus results in the production of 13-.beta. ivermectin monoglucopyranoside as the major product and of 5-.beta. ivermectin monoglucopyranoside as the minor product.Type: GrantFiled: October 15, 1990Date of Patent: December 3, 1991Assignee: Merck & Co., Inc.Inventors: Brian R. Petuch, Shieh-Shung T. Chen, Edward S. Inamine, Raymond F. White
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Patent number: 4994381Abstract: Disclosed herein are N-[[6-methoxy-5-(trifluoromethyl)-1-naphthalenyl]-thioxmethyl]-N-methylgly cine S-oxide (tolrestat S-oxide) and the amine thereof and methods of their preparation. The S-oxides are new aldose reductase inhibitors useful for the treatment or prevention of diabetic complications.Type: GrantFiled: March 20, 1989Date of Patent: February 19, 1991Assignee: American Home Products CorporationInventors: Eckhardt S. Ferdinandi, Michael S. Malamas, Kazimir Sestanj, Surendra N. Sehgal
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Patent number: 4898822Abstract: A process for preparing optically active indoline-2-carboxylic acid by an optical resolution, which comprises subjecting a racemic ester of (R,S)-indoline-2-carboxylic acid having the general formula [(R,S)-I] to the action of an enzyme or a microorganism having a stereo-selective esterase activity, which is capable of asymmetrically hydrolyzing the racemic ester [(R,S)-I] to give optically active indoline-2-carboxylic acid having the formula [II*] so as to produce the hydrolysis product, i.e. optically active indoline-2-carboxylic acid [II*] and an unreacted optically active ester of indoline-2-carboxylic acid having the general formula [I*], isolating each optically active form, and further, if necessary, hydrolyzing the obtained optically active ester [I*] to give an optical antipode of the acid [II*].According to the process of the present invention, optically active indoline-2-carboxylic acid with a high optical purity can be prepared in a simple process with a good yield.Type: GrantFiled: March 31, 1986Date of Patent: February 6, 1990Assignee: Kanegafuchi Kagaku Kogyo Kabushiki KaishaInventors: Masanori Asada, Shigeki Hamaguchi, Hidetoshi Katsuki, Yoshio Nakamura, Hideyuki Takahashi, Kenji Takahara, Yoshio Shimada, Takehisa Ohashi, Kiyoshi Watanabe
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Patent number: 4798792Abstract: A microbial process for converting codeine to 14-hydroxycodeine is provided. This process comprises aerobically culturing codeine with bacteria of the genus Streptomyces for at least about 3 days in a rich medium such as soybean flour medium.Type: GrantFiled: September 9, 1985Date of Patent: January 17, 1989Assignee: E. I. Du Pont de Nemours and CompanyInventors: Patricia A. Harder, Daniel A. Kunz
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Patent number: 4734367Abstract: The manufacture of optically active compounds of the formula ##STR1## wherein R.sup.1 signifies alkyl, phenyl or benzyl and R.sup.2 signifies hydrogen or a customary ester residue,by the fermentative reduction of compounds of the formula ##STR2## wherein R.sup.1 and R.sup.2 have the above significance. The compounds obtained are valuable intermediates in organic syntheses.Type: GrantFiled: November 23, 1984Date of Patent: March 29, 1988Assignee: Hoffmann-La Roche Inc.Inventors: Hans G. W. Leuenberger, Peter K. Matzinger, Dieter Seebach, Max F. Zuger
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Patent number: 4546079Abstract: An antibiotic compound which can be in the iron-free or iron-containing form and has the structural formula (B) as described herein. The compound possesses antimicrobial activity. Also fermentation processes for preparation of the above-mentioned antibiotic compound. In addition, pharmaceutical compositions containing said antibiotic compound and the use of said antibiotic compound and compositions for combating bacterial infection.Type: GrantFiled: May 31, 1983Date of Patent: October 8, 1985Assignee: Bayer AktiengesellschaftInventors: Karl G. Metzger, Jorg Pfitzner, Delf Schmidt, Horst Weyland, Gunter Benz, Theo Schroder
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Patent number: 4438197Abstract: The antibacterial compound DC-38-V is produced by culturing a microorganism belonging to the genus Streptomyces.Type: GrantFiled: April 16, 1982Date of Patent: March 20, 1984Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Fusao Tomita, Yuzuru Matsuda, Kunikatsu Shirahata, Keiichi Takahashi, Hirofumi Nakano, Tomoyasu Sato, Shuji Okubo, Nobuo Nakamura
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Patent number: 4410626Abstract: 7.alpha.-Methoxy-3-p-sulfooxy or p-hydroxycinnamoyloxymethyl cephalosporin derivatives which are useful as antibiotics and as intermediates for the production of other 7.alpha.-methoxycephalosporin derivatives.Type: GrantFiled: October 29, 1980Date of Patent: October 18, 1983Assignee: Yamanouchi Pharmaceutical Co. Ltd.Inventors: Hiroshi Gushima, Shunichi Watanabe, Takeshi Saito, Toshio Sasaki, Hideo Eiki, Yoshihiko Oka, Takashi Osono
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Patent number: 4330624Abstract: The antibiotic Tunicamycin is produced by fermenting a Tunicamycin-producing strain of Streptomyces (Streptomyces griseus ATCC 31591) under controlled conditions in a suitable nutrient medium and isolating the antibiotic so produced from the fermentation medium.Type: GrantFiled: December 3, 1980Date of Patent: May 18, 1982Assignee: Merck & Co., Inc.Inventors: Enrique Tejera, Sara A. Currie, James E. Flor, Richard L. Monaghan
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Patent number: 4212941Abstract: 9-(2-O-Acyl-.beta.-D-arabinofuranosyl)adenine compounds and their production by enzymatic removal of the 3-O-acyl and 5-O-acyl groups of a 9-(2,3-di-O-acyl-.beta.-D-arabinofuranosyl)adenine compound or a 9-(2,3,5-tri-O-acyl-.beta.-D-arabinofuranosyl)adenine compound. The monoester compounds are useful as antiviral agents. The compounds are water-soluble and lipophilic, thereby being adaptable to a wide variety of pharmaceutical formulations.Type: GrantFiled: May 23, 1977Date of Patent: July 15, 1980Assignee: Warner-Lambert CompanyInventor: David C. Baker