Abstract: An aqueous solution for suspending and storing cells, especially erythrocytes, and containing sodium chloride, glucose or fructose, adenine, and a sugar alcohol, the sugar alcohol being sorbitol or xylitol is employed to further improve the storage time and hence the survival rate of the cells and even reduce the hemolysis rate of erythrocytes in packed red blood cells.
Abstract: A tonometer having vessels for producing and maintaining multi-levels of tonometerd aqueous buffered solutions and whole blood available for use in the quality controlling of a blood-gas analyzer. A water bath maintains all vessels at a predetermined temperature and gas mixtures are supplied at a very low rate thereby permitting continuous operation of the tonometer.
Abstract: An isotonic multipurpose blood diluent, and a method for use of this diluent with a weak lysing reagent system which is especially suitable for routine enumeration of traditional hemogram values, and also the determination of lymphoid-myeloid populations of leukocytes, particularly in automatic particle counting systems.This blood diluent is capable of affording accurate, reproducible test results. It is an osmotically balanced aqueous solution of preselected pH containing Procaine hydrochloride for maintaining erythrocyte morphology during operation, N-(2-acetamido)iminodiacetic acid (ADA) as a blood cell stabilizing agent, and bacteriostatic agents including sodium 1-hydroxypyridine-2-thione, and dimethylolurea which, together with the ADA, allow preferential determination of myeloid-lymphoid leukocytes, and other hematological values.The lysing agent is a mixture of an aqueous solution of at least one quaternary ammonium salt having surface active properties, and an alkali metal cyanide.
Type:
Grant
Filed:
July 6, 1982
Date of Patent:
June 4, 1985
Assignee:
Coulter Electronics, Inc.
Inventors:
James H. Carter, Stephen L. Ledis, Harold R. Crews
Abstract: Viruses, particularly hepatitis viruses, in blood clotting enzyme compositions are inactivated with little enzyme activity loss by heating the compositions in the dry state. The novel products which result are therapeutically and diagnostically useful.
Abstract: This invention encompasses methods and reagents for inactivation of bacterial growth inhibitors present in blood, serum or plasma. It has been found that salicylates and closely related compounds will neutralize bacterial growth inhibitors present in the blood of many patients. The addition of salicylate to conventional growth medium also provides a reagent for monitoring the antibiotic levels in blood by enabling measurement of the effect of the antibiotic against a standard test organism without interference from the bacterial growth inhibitor present in sera.
Type:
Grant
Filed:
May 3, 1982
Date of Patent:
November 20, 1984
Assignee:
Abbott Laboratories
Inventors:
George M. Fukui, Herbert J. Spencer, Laurens R. Williams, II
Abstract: A process for quantitatively assaying factor Xa from a medium by reacting the medium with tripeptide derivatives having the formula ##STR1## wherein R.sup.5 is a chromogenic substituted amino group capable of being split off by enzymatic hydrolysis to form a colored or fluorescent product H--R.sup.5. The quantity of split product H--R.sup.5 released by the enzymatic action on the tripeptide derivative is determined photometrically, spectrophotometrically, fluorescence-spectrophotometrically, or electrochemically. The quantity of released split product H--R.sup.5 per time unit is proportional to the quantity of enzyme present in the starting material.
Abstract: Biological indicators are used to evaluate the effectiveness of virus inactivation conducted on virus-contaminated, protein-containing compositions. The indicators comprise dry protein and a predetermined titer of infectious virus.
Abstract: A reference fluid for the calibration and control of blood gas instruments is comprised of substantially pure stroma-free hemoglobin solution, a buffering agent, a source of bicarbonate ion and predetermined amounts of gases found in in vivo blood.
Abstract: A control material for use in the determination of glycosylated and total hemoglobin, and a method of preparing the same, are disclosed. The control is prepared by the steps of hemolyzing red blood cells, preferably in the presence of a detergent, followed by removal of stroma and lipids from the hemoglobin with an organic solvent such as toluene in the presence of a polyhydroxy compound such as sucrose. A hemoglobin-rich fraction is separated from the resulting mixture and dialyzed against a solution of a polyhydroxy compound. The dialysate may be lyophilized to provide a stable, dry product suitable for reconstitution.
Type:
Grant
Filed:
July 16, 1981
Date of Patent:
August 14, 1984
Assignee:
Sherwood Medical Company
Inventors:
John J. Huang, Jung-Shou Chen, Louis M. Mezei
Abstract: Disclosed are cell-free, stable, aqueous blood gas controls containing a proteinaceous foaming agent. The addition of foaming agent to the blood gas control permits generation of a gas impermeable foam within the head space of a sealed ampule in which the control is packaged. Upon agitation of the ampule prior to opening, the foam thus generated effectively isolates the control fluid from the ambient environment, thereby maintaining the concentration of gases dissolved within the fluid at their assigned values.
Abstract: Artificially constituted storage-stable hemoglobin compositions for use in the temperature correction of a cation exchange column chromatographic assay for hemoglobin A.sub.1 or A.sub.1c in a sample of human blood. The relative amounts of hemoglobin components in the composition conform approximately to the following formula:______________________________________ y = mx + b where y = weight percent A.sub.1c with respect to A.sub.1 x = weight percent A.sub.1 with respect to total hemoglobin m = -1.7 and b = 85 when x < 10, and m = -1.2 and b = 80 when x > 10. ______________________________________The compositions when analyzed in a cation exchange assay produce a result which varies with temperature in substantially the same manner as that from a true sample of human blood.
Type:
Grant
Filed:
August 24, 1982
Date of Patent:
May 15, 1984
Assignee:
Bio-Rad Laboratories, Inc.
Inventors:
Dennis M. Bleile, Diane M. Allen, Steve K. Tanaka, Francis J. Matarrese, Jr.
Abstract: A consistent, stable serum having good optical qualities and improved filterability suitable for control and calibration uses is disclosed as well as its method of preparation.
Abstract: An acidic solution of glycine and a fatty acid of 3 to 12 carbon atoms provides an improved solution for cleaning and storing blood dialyzers and other separation devices, particularly those intended for contact with blood after they have once been used.
Abstract: A method is disclosed of blood serum sample preparation for improved, more accurate and precise, electro-optical method for measuring erythrocyte volumes, individually and as an average.
Abstract: A method for the removal of hormones from whole human serum which comprises: contacting, at a pressure higher than 1 psi the serum with a composite sheet, the composite sheet comprising a matrix of self-bonding fibers having interdispersed therein carbon particles, such that better than 90% of the carbon particles have an average diameter less than about 50 microns; and recovering a whole human serum having substantially undetectable amounts of hormones. The method is particularly useful for the removal of thyroid hormones.
Abstract: A method and system for measuring the oxygen carrying capacity of normal and abnormal hemoglobins and red cells under controlled pH, ionic strength, temperature and gas conditions is described. A blood sample and a physiologically balanced medium are placed in a cuvette and means are associated therewith for measuring the fractional saturation of the sample and the partial pressure of the oxygen (sometimes referred to as the oxygen tension) and provide output signals to an X-Y plotter for continuous recording. Preferably, means for maintaining the temperature of the medium and the blood sample is provided.
Abstract: Synthetic stool, adaptable to use as a control standard in the diagnostic detection of substances contained in stools and for demonstration purposes, said stool comprising a matrix of a member selected from the group consisting of oxygen-containing organic polymers which are difficultly soluble in water and oxygen-containing inorganic solids difficultly soluble in water, a non-bleeding coloring agent adherent to said matrix and simulating the color of a stool, and a liquid selected from the group consisting of water and lubricants.
Abstract: A reference liquid for calibration or quality control of instruments which determine ionized calcium and pH. The reference liquid contains both a particular calcium ion activity and a pH-buffer and can therefore be used for calibration of both the calcium-sensitive electrode and the pH-electrode in the instruments. The pH-buffer is a nitrogen-containing organic sulphonic acid and the salt of this acid, the acid having a pK in the range of 6.6-7.6. The reference liquid has an ionic strength of 0.15-0.17, a buffer capacity .beta. in the range of 0.04-0.10 and is packed in glass ampoules.