Cellulose Or Derivative Patents (Class 436/530)
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Patent number: 7476548Abstract: An assay device, analytical instrument and assay method for determining the presence or amount of an analyte in a fluid is disclosed. The device is a one-step lateral flow dry reagent immunoassay with one, two or more zones along a transverse axis of the device, each zone can contain or be preceded by diffusively or non-diffusively bound reagents. The invention measures an indicator in one or two test zones for each analyte to determine its presence or concentration. The signal reagent (indicator) may be a particle such as a colored latex or colloidal gold. The assay quantitation may be read by an instrument.Type: GrantFiled: March 31, 2004Date of Patent: January 13, 2009Assignee: Bayer Healthcare LLCInventors: Joel M. Blatt, Wilma M. Mangan
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Patent number: 7473563Abstract: Provided is sampling and testing device for the detection of specific molds, allergens, viruses, bacteria, fungi, and other protein containing substances. Embodiments of the device include a sampling member slideably engaged with a base that contains a lateral flow strip adapted to detect specific analytes of interest. The sampling member defines a solvent reservoir that stores an elution solvent in a fluid-tight manner before the device is used to sample and test environmental surfaces. During slideable withdrawal of the sampling member from the base, the elution solvent stored in the reservoir is automatically released to a wick assembly of the sampling member. The wick assembly includes a wick adapted to receive, distribute, and retain the elution solvent. After a user samples an environmental surface for an analyte of interest with the elution solvent wetted wick, the sampling member is returned to the base where the wick contacts the lateral flow strip contained in the base.Type: GrantFiled: April 3, 2006Date of Patent: January 6, 2009Assignee: The Clorox CompanyInventors: Brendi M. Cumberland, Maha El-Sayed, Alan J. Fujii, Scott D. Manske, Elias A. Shaheen
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Publication number: 20080305559Abstract: The present invention provides noncompetitive immunoassays to detect small molecules.Type: ApplicationFiled: December 15, 2006Publication date: December 11, 2008Inventors: Gualberto Gonzalez-Sapienza, Bruce Hammock, Andres Gonzalez-Techera, Lucia Vanrell, Hee Joo Kim
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Patent number: 7445941Abstract: The assay devices, assay systems and device components of this invention comprise at least two opposing surfaces disposed a capillary distance apart, at least one of which is capable of immobilizing at least one target ligand or a conjugate in an amount related to the presence or amount of target ligand in the sample from a fluid sample in a zone for controlled fluid movement to, through or away the zone. The inventive device components may be incorporated into conventional assay devices with membranes or may be used in the inventive membrane-less devices herein described and claimed. These components include flow control elements, measurement elements, time gates, elements for the elimination of pipetting steps, and generally, elements for the controlled flow, timing, delivery, incubation, separation, washing and other steps of the assay process.Type: GrantFiled: March 2, 2004Date of Patent: November 4, 2008Assignee: Biosite IncorporatedInventor: Kenneth F. Buechler
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Patent number: 7439025Abstract: Antibodies are described, which are suitable for identification of ?-carboxyglutamic acid (Gla), said antibodies having an ability of identifying Gla residues in proteins and/or peptides, while not reacting with glutamic (Glu) residues in corresponding proteins and/or peptides. Methods for the preparation and identification of said antibodies as well as methods for detection of Gla in biological fluids, tissue extracts, tissue specimens, in which methods said antibodies are used, are also described. There is also described the use of said antibodies for immunopurification of Gla-containing proteins or peptides by, for instance, immunoprecipitation or immunoaffinity chromatography.Type: GrantFiled: March 1, 2001Date of Patent: October 21, 2008Assignee: Protease ABInventors: Johan Stenflo, Loisa Mary Stenberg, Mark Alan Brown
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Patent number: 7432111Abstract: A non-continuous immunoassay device which includes two or more separated pads for immunoassay analysis, and is capable of controlling the migration speed of a mobile phase between the separated pads, and an immunoassay method using the same are disclosed. The immunoassay device includes a first pad receiving a mobile phase; a second pad which is spatially separated from the first pad by a predetermined distance, and to which the mobile phase migrates; an upper case for covering the upper parts of the first pad and the second pad; a lower case for covering the lower parts of the first pad and the second pad; and a connecting member which is formed on at least one of the upper case and the lower case, and located between the first pad and the second pad to form a passage for moving the liquid sample.Type: GrantFiled: July 28, 2005Date of Patent: October 7, 2008Assignee: Standard Diagnostics, Inc.Inventors: Young-Shik Cho, Hyo-Keun Lee, Byung-Ki Cho
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Patent number: 7427511Abstract: An immunochromatographic test device of the present invention comprises: a sample receiving member for receiving a sample; a label holding member for holding a labeling substance to bind to an analyte contained in the sample; and a chromatographic membrane having a detection zone at which an immobilization substance to bind to the analyte is immobilized, wherein the sample receiving member disposed to cover the label holding member and in contact with the chromatographic membrane, and the chromatographic membrane is spaced apart from the label holding member.Type: GrantFiled: January 5, 2006Date of Patent: September 23, 2008Assignee: Sysmex CorporationInventors: Masako Aki, Shinya Nagai, Noriyuki Saito, Takeshi Imoarai
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Publication number: 20080220538Abstract: The present invention relates to an antibody having specificity for a polypeptide comprising an amino acid sequence of SEQ ID No:2 or an isoform thereof or a polypeptide comprising SEQ ID NO:2.Type: ApplicationFiled: October 7, 2005Publication date: September 11, 2008Applicant: NATIONAL INSTITUTE OF IMMUNOLOGYInventor: Anil Kumar Suri
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Patent number: 7407796Abstract: A device and method allowing evaluation of the contents of a sealed primary container by means of an integral sensor which is separated from the contents of the sealed primary container yet provides information on quality of the contents of the primary container without breaking the sealed system. The integral sensor device includes a biosensor retained within a plastic construct by a gated-pore membrane. Pores in the membrane open in response to an environmental change in the primary container allowing the contents of the primary container to contact the biosensor. Status of the contents of the primary container can be determined by inspection of the biosensor, visually or via a fiberoptic probe, through the optical window of the plastic construct.Type: GrantFiled: May 6, 2004Date of Patent: August 5, 2008Assignee: The Penn State Research FoundationInventors: Roy H. Hammerstedt, Allen T. Phillips
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Publication number: 20080176342Abstract: The invention concerns a diagnostic kit for simultaneous assay of antibiotics of different classes. The kit contains a single reaction mixture containing at least one first labelled receptor, specific of recognition of ?-lactams, a second labelled receptor, specifically and competitively identifying a tetracycline and a biotinylated nucleic acid fragment, an antisulfamide antibody and a labelled sulfamide analogue; and a recovery system in the form of a solid support comprising a nitrocellulose membrane whereon are fixed in three separate testing zones, respectively an antibiotic with ?-lactam nucleus, an avidin and an antibody capable of specifically identifying the antisulfamide antibody, so that the labelling intensity detected on the recovery system at the three testing zones results independently from a competitive recognition of each antibiotic by its labelled receptor.Type: ApplicationFiled: April 13, 2006Publication date: July 24, 2008Inventor: Benoit Granier
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Patent number: 7402381Abstract: Aspects of the invention can provide a method capable of easily realizing immobilization with the optimum density derived from a concentration control and without phase separation in coadsorption of a number of molecules. The immobilization method can include the step of dissolving a plurality of molecules to be immobilized to a solid phase substrate with a solvent to obtain a solution of the plurality of molecules, and the step of incubating the solution and the solid phase substrate in touch therewith. Each of the molecules can include a solid phase substrate joint portion having a jointing property to the solid phase substrate, a functional portion having a specific function, and a linker portion positioned between the solid phase substrate joint portion and the functional portion.Type: GrantFiled: August 27, 2004Date of Patent: July 22, 2008Assignee: Seiko Epson CorporationInventors: Hiroshi Takiguchi, Hitoshi Fukushima
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Patent number: 7393696Abstract: This invention provides bovine pregnancy test methods and devices. The test is also suitable for other ruminant and/or ungulate animals. Antigens from Group A (early pregnancy antigens), and/or Group B (mid-pregnancy antigens), and Group C (early, mid- and late pregnancy antigens) are detected in a fluid from the animal, and pregnancy is reliably determined. The pregnancy assays of this invention are preferably carried out using immunoassay devices which provide immediate results in the field.Type: GrantFiled: September 24, 2002Date of Patent: July 1, 2008Assignee: AspenBio Pharma, Inc.Inventors: Jay W. Roth, Mark Colgin, Roger Hurst, Diane Newman, Cathy Landmann
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Patent number: 7385022Abstract: Disclosed is a polythiocarbonate polythiol including repeating units represented by the chemical formula (I): in which formula (I), R represents a divalent hydrocarbon group, which hydrocarbon group may have at least one substituent which does not take part in a reaction with the polyisocyante component and the carbon chain in the hydrocarbon group may include at least one member selected from hetero atoms and an —OCO— group, and the polythiocarbonate polythiol has a number average molecular weight of 200 to 2500.Type: GrantFiled: January 4, 2007Date of Patent: June 10, 2008Assignee: Ube Industries, Ltd.Inventors: Masanori Watanabe, Masahiko Watanabe, Atsushi Morikami, Takafumi Hirakawa
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Patent number: 7371583Abstract: Disclosed is a testing device and methods for the identification of an analyte of interest in a sample. In a preferred embodiment, the testing device includes a front panel having at least one sample application aperture; a rear panel having at least one solvent application aperture; a sample collection matrix disposed between the rear panel and the front panel, the sample collection matrix being in communication with the sample and solvent application apertures of the front and rear panels; and at least one insertable test strip containing a reagent enabling detection of the analyte of interest.Type: GrantFiled: March 11, 2005Date of Patent: May 13, 2008Assignee: Enterix, Inc.Inventor: Howard Chandler
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Patent number: 7368296Abstract: Solid supports for chemiluminescent assays are provided. The solid support includes a plurality of probes covalently or physically attached to the support surface and a chemiluminescent enhancing moiety incorporated onto the surface or into the bulk of the support. The solid support can be a multi-layered support including an upper probe binding layer (e.g., an azlactone polymer layer or porous functional polyamide layer) adjacent to a cationic microgel layer. The azlactone-functional polymer can be a copolymer of dimethylacrylamide and vinylazlactone crosslinked with ethylenediamine. The cationic microgel layer can be a cross-linked quaternary onium salt containing polymer. A method and a kit for conducting chemiluminescent assays using the solid supports is also provided. The kit comprises a dioxetane substrate, a biopolymer probe-enzyme complex, and a solid support.Type: GrantFiled: January 17, 2002Date of Patent: May 6, 2008
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Patent number: 7364898Abstract: The present invention relates to a detection and quantification method of different sets of target molecules (1, 3), being possibly present simultaneously in a biological sample by the use of a microarray present upon a solid support surface (6) and comprising different sets (A, B) of capture molecules (2, 4), present in different locations (8, 9) of the solid support surface (6).Type: GrantFiled: May 4, 2004Date of Patent: April 29, 2008Assignee: Eppendorf AGInventors: Lars-Erik Peters, José Remacle, Konrad Beyreuther
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Patent number: 7312028Abstract: A simple easy to manufacture analytical device capable of performing membrane based immunoassays on batch of samples within 3 to 10 minutes wherein the method permits focused application of samples, costly labeled immunoassay and signal amplification reagents, said device includes an antibody-immobilized micro porous membrane, breadth corner layer of which is directly attached to a semi-rigid liquid-impervious body with water insoluble adhesive; absorbent body is provided separately and is not attached to analytical device during manufacture, absorbent body is wetted and is placed proximal to the lower surface of the membrane thereby forming networks of capillary channels with the absorbent body; flow of samples or reagents is always kept downwards and focused without application of any force to the absorbent body and the use of disposable adsorbent body permits stepwise addition of signal amplification reagents for ultra sensitive detection of diagnostically important molecules by visual examination of the mType: GrantFiled: July 28, 2006Date of Patent: December 25, 2007Assignees: Council of Scientific & Industrial Research, Department of Biotechnology, A Department of Government of IndiaInventors: Tarun K. Dhar, Arindam Pal
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Patent number: 7309611Abstract: A test strip and method for detecting an analyte present in a sample.Type: GrantFiled: June 14, 2004Date of Patent: December 18, 2007Assignee: Relia Diagnostic Systems, LLCInventors: Robert K. DiNello, Alan J. Polito, Stella S. Quan
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Patent number: 7285426Abstract: The present invention provides methods, compositions, and kits for rapid detection of analytes in a sample. Surprisingly, it has been found that bulking materials having starch reagents effectively enhance chromatographic detection methods by providing component stability and controlled release. Thus, the novel components and methods disclosed herein provide a completely new modality of chromatographic analyte detection.Type: GrantFiled: May 5, 2005Date of Patent: October 23, 2007Assignee: Medtox Scientific, Inc.Inventors: Robert Bohannon, Glen Chapman, Alan Morris, Maria Scholz-Steele, Martin Green, Phillip Hartzog, Oluyomi Adeneye
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Patent number: 7270995Abstract: The present invention is an analytical device which comprises a porous piece assembly consisting of a liquid reagent-receiving porous piece (1), a labeled substance-retaining piece (2), a test piece (3) comprising a detection site (4) and a reference site (5), and a sample-absorbing porous material piece (6); and a sample-receiving porous material piece (7) disposed independently therefrom and partially communicated therewith through a connection. The analytical device of the present invention exhibits extremely high sensitivity and can accurately perform various types of analysis.Type: GrantFiled: December 19, 2000Date of Patent: September 18, 2007Assignee: Nichirei BiosciencesInventors: Hirohisa Matsushita, Hidenori Kawai, Takashi Kitoh
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Patent number: 7267993Abstract: Methods and compositions for identifying and characterizing one or more ligands of a peptide are provided. In particular, the invention provides a phage ligand sensor device (PLSD) comprising a sensor coupled to a binding element of interest. Binding elements of interest comprise phage displaying at least one foreign peptide. The PLSD and assays find particular use in identifying and characterizing ligand-peptide interactions.Type: GrantFiled: November 7, 2002Date of Patent: September 11, 2007Assignee: Auburn UniversityInventors: Valery A. Pentrenko, Vitaly J. Vodyanoy, Alexandre M. Samoylov, Iryna Sorokulova, Viswaprakash Nanduri, Bryan A. Chin, James M. Barbaree, W. Charles Neely
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Patent number: 7241626Abstract: Methods, articles, and kits for obtaining elevated concentrations of a substance from a sample that has become bound, for example by providing a positive test result in an immunoassay or other binding assay. The concentrated substance is optionally subjected to further processing or analysis, for example to confirm the identity of the substance, such as a pathogenic organism in a food sample.Type: GrantFiled: November 12, 2003Date of Patent: July 10, 2007Assignee: Strategic Diagnostics Inc.Inventor: Jingkun Li
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Patent number: 7238537Abstract: An analytical test device incorporating a dry porous carrier to which a liquid sample, eg. urine, suspected of containing an analyte such as HCG or LH can be applied indirectly, the device also incorporating a labelled specific binding reagent which is freely mobile in the porous carrier when in the moist state, and an unlabelled specific binding reagent which is permanently immobilized in a detection zone on the carrier material, the labelled and unlabelled specific binding reagents being capable of participating in either a sandwich reaction or a competition reaction in the presence of the analyte, in which prior to the application to the device of a liquid sample suspected of containing the analyte, the labelled specific binding reagent is retained in the dry state in a macroporous body, eg.Type: GrantFiled: September 4, 2001Date of Patent: July 3, 2007Assignee: Inverness Medical Switzerland GmbHInventors: Paul James Davis, Michael Evans Prior, Keith May
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Patent number: 7232661Abstract: This invention is about a method for the prenatal diagnosis of preterm delivery, fetal infection, and fetal damage, and diagnostic reagent system and diagnostic kit for the diagnosis. The method, diagnostic reagent system, and kit are based on the finding that the level of MMP-8 in the amniotic fluid is significantly higher when the pregnant woman is at risk for preterm delivery, intrauterine infection, and fetal damage. The diagnostic reagent system and kit can be applied to patients with or without clinical signs of preterm labor or premature rupture of fetal membranes. With its superiority in sensitivity and specificity as well as its less invasiveness compared to the conventional method of measuring fetal blood cytokine levels, this diagnostic reagent system and kit is very useful in the prenatal diagnosis of preterm delivery, fetal infection, and fetal damage.Type: GrantFiled: August 1, 2001Date of Patent: June 19, 2007Assignee: Seoul National University Industry FoundationInventor: Bo Hyun Yoon
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Patent number: 7229839Abstract: A test strip adapted to receive a sample and detect an analyte therein is provided. The test strip comprises a sample addition zone to which a sample may be added; an absorbent zone proximal to the sample addition zone; one or more test zones distal to the sample addition zone, at least one of the test zones including a first analyte binding agent immobilized therein which is capable of binding to the analyte to be detected; and a terminal sample flow zone distal to the one or more test zones, the absorbent zone being positioned relative to the sample addition zone and having an absorption capacity relative to the other zones of the test strip such that a distal diffusion front of a sample added to the sample addition zone diffuses from the sample addition zone to a distal diffusion point within the terminal sample flow zone and then reverses direction and diffuses proximal relative to the one or more test zones.Type: GrantFiled: January 17, 2003Date of Patent: June 12, 2007Assignee: Relia Diagnostic Systems, LLCInventors: Richard M. Thayer, Alan J. Polito, Robert K. Dinello, George H. Sierra, Henry J. Wieck
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Patent number: 7229835Abstract: A compound linked to a solid support (R) through a divalent linker moiety (X) and which is represented by the following formula: is disclosed. In particular, the 1-hydroxybenzotriazole-6-carboxylic acid is directly linked to the support under mild conditions (i.e., in aqueous or organic solvents at neutral pH and at room temperature). The polymer bound 1-hydroxybenzotriazole-6-carboxylic acid can be used for the derivatization of amines as well as for single step amino group modification of proteins, peptides, and amines via acylation or sulfonylation reactions. A flow through device and method for the single step amino group modifications of proteins, peptides, and amines is disclosed. Also disclosed is a flow through device for the detection of amines in a sample. Additionally, a device and method for the detection of amines in a sample using 1-hydroxybenzotriazole-6-carboxylic acid is disclosed. In a preferred embodiment, the device is used to detect the presence of amines in a spoiled meat product.Type: GrantFiled: October 25, 2001Date of Patent: June 12, 2007Assignee: The University of Maryland, Baltimore CountyInventor: Aristotle G. Kalivretenos
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Patent number: 7226793Abstract: A test device and method for determining the presence or absence of an analyte in a fluid sample, the test device including a support bearing a mark thereon, and a matrix defining an axial flow path. In operation, an observation area in the test device becomes transparent, thereby allowing the user to view a mark that is present on a support that is disposed beneath the observation area. Typically, the mark on the underlying support is configured as a minus (?) sign. In the absence of analyte in the sample, the test device presents a negative result as a minus (?) signal. In the presence of analyte in the sample, however, the mark operates in concert with a perpendicular test line on the observation area to present a positive result as a plus (+) signal that is visible to the user.Type: GrantFiled: June 12, 2003Date of Patent: June 5, 2007Assignee: Quidel CorporationInventors: Jeremy Jerome, Mark Daquipa, Bruce Jacono, Hans Boehringer
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Patent number: 7214531Abstract: An assay system has a chamber that receives a test strip onto which a sample comprising an analyte has been placed. The chamber is in gaseous communication with a piezoelectric material that generates an electrical signal in response to a pressure change in the chamber that is caused by a reaction between the analyte and a reagent.Type: GrantFiled: April 15, 2002Date of Patent: May 8, 2007Assignee: Disan, Inc.Inventors: Mahmoud Ladjevardi, Theodore Sand
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Patent number: 7211445Abstract: A method is provided for covalently linking carbohydrates, proteins, nucleic acids, and other biomolecules under neutral conditions, using a Diels-Alder cycloaddition reaction. In an example, activated carbon-carbon double bonds were attached to free amino sites of a carrier protein, and a conjugated diene was attached to a carbohydrate hapten. Spontaneous coupling of the carbohydrate and the protein components under very mild conditions provided glycoconjugates containing up to 37 carbohydrate hapten units per carrier protein molecule. The method is also applicable to the immobilization of biomolecules on gel or solid supports. The conjugated products are useful as immunogens and as analytical and diagnostic reagents.Type: GrantFiled: October 22, 2003Date of Patent: May 1, 2007Assignee: The United States of America as Represented by the Secretary of the Department of Health and Human ServicesInventor: Vince Pozsgay
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Patent number: 7205159Abstract: A method and apparatus for use in a flow through assay process is disclosed. The method is characterised by a “pre-incubation step” in which the sample which is to be analysed, (typically for the presence of a particular protein), and a detection analyte (typically an antibody bound to colloidal gold or a fluorescent tag) which is known to bind to the particular protein may bind together for a desired period of time. This pre incubation step occurs before the mixture of sample and detection analyte come into contact with a capture analyte bound to a membrane. The provision of the pre-incubation step has the effect of both improving the sensitivity of the assay and reducing the volume of sample required for an assay. An apparatus for carrying out the method is disclosed defining a pre-incubation chamber for receiving the sample and detection analyte having a base defined by a membrane and a second membrane to which a capture analyte is bound.Type: GrantFiled: August 20, 2002Date of Patent: April 17, 2007Assignee: Proteome Systems Intellectual Property Pty Ltd.Inventors: Robert Alan Cole, Andrew John Sloane, William Samuel Hunter
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Patent number: 7179657Abstract: A test device and method for determining the presence or absence of one or more analytes in a fluid sample, the test device including a support or member bearing a mark thereon, and a matrix or member containing a capture zone. In operation, an observation area in the test device becomes transparent, thereby allowing the user to view a mark that is present on a support that is disposed beneath the observation area. Typically, the mark on the underlying support is configured as a minus (?) sign. In the absence of analyte in the sample, the test device presents a negative result as a minus (?) signal. In the presence of analyte in the sample, however, the mark operates in concert with a perpendicular test line on the observation area to present a positive result as a plus (+) signal that is visible to the user.Type: GrantFiled: September 10, 2002Date of Patent: February 20, 2007Assignee: Quidel CorporationInventors: Jeremy Jerome, Mark Daquipa, Bruce Jacono, Hans Boehringer, Paul Lambotte, Paul J. Lawrence
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Patent number: 7176036Abstract: Methods and devices for electrochemical detection of a specific binding pair member utilizing a microsphere with an incorporated electroactive marker, wherein a member of the specific binding pair to be detected is bound, directly or through one or more intermediates, to the microsphere. Multiple specific binding pair members may be detected by use of electrochemically distinguishable electroactive markers. Microspheres with incorporated electroactive markers may include one or more functional groups for binding members of specific binding pairs, and are preferably insoluble in aqueous solvents but soluble in selected organic solvents.Type: GrantFiled: September 18, 2003Date of Patent: February 13, 2007Assignee: Arrowhead Center, Inc.Inventors: Joseph Wang, Ronen Polsky, Kathryn L. Turner
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Patent number: 7169618Abstract: An improved method for separating materials is provided, using colloidal, magnetizable aggregates, optionally silanized, and coated with a one or more layers of novel polysaccharide derivatives. Materials separated by the aggregates of the invention include inorganic and organic molecules, viruses, organelles, and cells. The invention also relates to a kit for separating such materials. The separated materials are useful in analytical and preparative or in diagnostic and therapeutic techniques.Type: GrantFiled: June 26, 2001Date of Patent: January 30, 2007Assignee: Skold TechnologyInventor: Carl Nelson Skold
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Patent number: 7144705Abstract: Heart and brain fatty acid binding proteins (H-FABP, B-FABP) are markers for stroke. The invention provides a diagnostic assay for either of these markers, preferably by ELISA using anti-H-FABP or B-FABP antibody. Since H-FABP is also a marker for acute myocardial infarction (AMI), to distinguish stroke from AMI requires an assay specific to AMI, e.g. using troponin-I or creatine kinase-MB as a marker, also to be carried out.Type: GrantFiled: June 7, 2002Date of Patent: December 5, 2006Assignee: ElectroPhoretics LimitedInventors: Denis Francois Hochstrasser, Jean-Charles Sanchez, Catherine Gabrielle Zimmerman
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Patent number: 7144743Abstract: For certain mixed mode resins having anionic character, a ligand is joined to a solid support via a linkage that includes a mercapto-, ether- or amino-containing moiety. A suitable ligand comprises an aromatic group, a heteroaromatic group, or a heterocyclic group, optionally fused, that is sulfate-, sulfonate-, phosphonate- or phosphate-substituted and that is linked to such a moiety. These resins possess an anionic character under conditions prescribed for their use. Separation of a biological substance, such as a peptide or protein, can be accomplished with a resin of this type via a change in the pH of eluants, thereby effecting adsorption and desorption.Type: GrantFiled: September 12, 2003Date of Patent: December 5, 2006Assignee: Pall CorporationInventors: Egisto Boschetti, Pierre Girot
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Patent number: 7122381Abstract: A selective adsorption material made by the process comprising: a) non-cavalently or reversibly-covalently binding a print molecule to at least two styrene, acrylate or silica monomers, each of which monomers brinds to said print molecule by means of a different functional group; b) immobilizing said bound monomers by being polymerized to each other in the presence of an effective amount of cross-linker; and c) removing said print molecule from said polymer by extraction to leave a cavity in said polymer which is stereo-tailored to a biological molecule of interest; and wherein said print molecule is the biological molecule of interest.Type: GrantFiled: September 4, 1992Date of Patent: October 17, 2006Inventors: Magnus Glad, Maria Kempe, Klaus Mosbach
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Patent number: 7112453Abstract: This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.Type: GrantFiled: August 5, 2002Date of Patent: September 26, 2006Assignee: Ciphergen Biosystems, Inc.Inventors: T. William Hutchens, Tai-Tung Yip
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Patent number: 7109042Abstract: An analytical test device useful for example in pregnancy testing, comprises a hollow casing (500) constructed of moisture-impervious solid material, such as plastics materials, containing a dry porous carrier (510) which communicates indirectly with the exterior of the casing via a bibulous sample receiving member (506) which protrudes from the casing such that a liquid test sample can be applied to the receiving member and permeate therefrom to the porous carrier, the carrier containing in a first zone a labelled specific binding reagent is freely mobile within the porous carrier when in the moist state, and in a second zone spatially distinct from the first zone unlabelled specific binding reagent for the same analyte which unlabelled reagent is permanently immobilised on the carrier material and is therefore not mobile in the moist state, the two zones being arranged such that liquid sample applied to the porous carrier can permeate via the first zone into the second zone, and the device incorporating meaType: GrantFiled: February 12, 2001Date of Patent: September 19, 2006Assignee: Inverness Medical Switzerland GmbHInventors: Keith May, Michael Evans Prior, Ian Richards
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Patent number: 7109024Abstract: This invention features a biomolecule-bound substrate that includes a support made of an organic polymer and having an unmodified surface; and a plurality of unmodified biomolecules immobilized on the unmodified surface. The organic polymer is acrylic resin, polypropylene, polystyrene, polyethylene, polyvinyl chloride, polysulfone, polycarbonate, cellulose acetate, rubber, latex, polyethylene terephthalate, acrylonitrile butadiene styrene, acrylonitrile styrene, or a combination thereof. The substrate is formed by placing the unmodified biomolecules on the unmodified surface followed by ultraviolet irradiation.Type: GrantFiled: April 7, 2003Date of Patent: September 19, 2006Assignee: Dr. Chip Biotechnology Inc.Inventors: Kan-Hung Lee, Yu-Hau Shih, Chuan-Mei Tsai, Yih-Weng Wang, Hsiung Hsiao, Chi-Horng Bair, Shin-Hwan Wang
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Patent number: 7090803Abstract: A lateral flow immunoassay test device that includes a housing with an elongated slot for holding a test sample collector; an elongated holder member for securing at least one immunoassay test strip therein; a first chamber for storing a first, pre-treatment reagent; and a second chamber for storing a second reagent. The pre-treatment reagent is contained within a rupturable enclosure. A piercing member is located within the housing that is used to rupture the enclosure in order to release the pre-treatment reagent so that the sample and pre-treatment reagent form a mixture. The second reagent may then be introduced to the mixture which is allowed to react with the second reagent for a period of time. The mixture and second reagent combination is then contacted with the immunoassay test strip.Type: GrantFiled: October 28, 2003Date of Patent: August 15, 2006Assignee: American Bio Medica CorporationInventors: Martin Gould, Yli Remo Vallejo, Robert Bernstine
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Patent number: 7087389Abstract: A simple easy to manufacture analytical device capable of performing membrane based immunoassays on batch of samples within 3 to 10 minutes wherein the method permits focused application of samples, costly labeled immunoassay and signal amplification reagents, said device includes an antibody-immobilized micro porous membrane, breadth corner layer of which is directly attached to a semi-rigid liquid-impervious body with water insoluble adhesive; absorbent body is provided separately and is not attached to analytical device during manufacture, absorbent body is wetted and is placed proximal to the lower surface of the membrane thereby forming networks of capillary channels with the absorbent body; flow of samples or reagents is always kept downwards and focused without application of any force to the absorbent body and the use of disposable adsorbent body permits stepwise addition of signal amplification reagents for ultra sensitive detection of diagnostically important molecules by visual examination of the mType: GrantFiled: February 9, 2001Date of Patent: August 8, 2006Assignees: Council of Scientific & Industrial Research, Department of Biotechnology, A Department of Govenment of IndiaInventors: Tarun K. Dhar, Arindam Pal
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Surface plasmon resonance biosensor for measurement of anti-glycolipid antibody levels in neuropathy
Patent number: 7074621Abstract: The present invention provides a method of detecting human antibodies in a sera solution. The invention also provides a method of quantitating anti-glycolipid antibody levels in solutions. The invention provides a method of diagnosing disease states, including neurological diseases, by quantitating a subject's antibody levels.Type: GrantFiled: May 3, 2004Date of Patent: July 11, 2006Assignee: The Trustees of Columbia University in the City of New YorkInventors: Norman Latov, Armin Alaedini -
Patent number: 7070941Abstract: The invention provides methods and compositions for azide tagging of biomolecules. In one embodiment of the invention, proteins are tagged by metabolic incorporation of prenylated azido-analog substrates. Examples of such analogs are azido farnesyl diphosphate and azido farnesyl alcohol. The azido moiety in the resulting modified proteins provides an affinity tag, which can be chemoselectively captured by an azide-specific conjugation reaction, such as the Staudinger reaction, using a phosphine capture reagent. When the capture agent is biotinylated, the resulting conjugates can be detected and affinity-purified by streptavidin-linked- HRP and streptavidin-conjugated agarose beads, respectively. The invention allows detection and isolation of proteins with high yield, high specificity, and low contamination without harsh treatment of proteins.Type: GrantFiled: November 17, 2003Date of Patent: July 4, 2006Assignee: Board of Regents, The University of Texas SystemInventors: Yingming Zhao, John R. Falck
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Patent number: 7067270Abstract: An object of the present invention is to provide a method for detecting a protein using a nonradioactive label which is prevented to a smaller extent than in the case of the interaction of biotin/(strepto)avidin, and which achieves higher detection sensitivity than that of the detection system using DIG. The present invention provides a method for detecting a target substance, which comprises steps of: (A) allowing a target substance to come into contact with a protein labeled with a compound having a 6-membered ring, so as to carry out a binding reaction; and (B) detecting the protein labeled with the compound having a 6-membered ring, which was bound to the target substance, by using an antibody against the above compound having a 6-membered ring.Type: GrantFiled: December 13, 2004Date of Patent: June 27, 2006Assignee: Fuji Photo Film Co., Ltd.Inventor: Yukio Sudo
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Patent number: 7067264Abstract: A test device (20) for detecting human blood includes a strip (22) having an introduction station (24), a test station (26), and a control station (28) disposed in spaced apart relationship. The test sample introduction station (24) has labeled antihuman Hb antibodies, the test station (26) has immobilized antihuman Hb antibodies, and the control station has immobilized polyclonal antibodies. A test sample (500) is deposited at the introduction station (24). If human hemoglobin is present in the test sample (500), a colored line will appear at the test station (26) and at the control station (28). If no human hemoglobin is present in the test sample (500), a colored line will only appear at the control station (28).Type: GrantFiled: July 23, 2001Date of Patent: June 27, 2006Inventor: Padma S. Bagaria
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Patent number: 7067324Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.Type: GrantFiled: January 12, 2001Date of Patent: June 27, 2006Assignee: Invitrogen CorporationInventors: Tom Knapp, Gregor Zlokarnik, Paul Negulescu, Roger Y. Tsien, Tim Rink
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Patent number: 7060505Abstract: An assay device and method are provided which allow the determination of the presence or absence of at least one analyte in a test sample, while providing specific identification of the test subject. The assay device includes a reaction medium having at least one reaction zone and at least one control zone, which is capable of providing a pattern suitable for identifying the test subject. The pattern suitable for identifying the test subject is preferably a fingerprint. In a preferred embodiment of the invention, the reaction zone and the control zone include at least one member of a ligand/receptor pair.Type: GrantFiled: March 8, 2004Date of Patent: June 13, 2006Assignee: La Mina, Inc.Inventor: Raouf Guirguis
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Patent number: 7052915Abstract: A method for selective labeling of phosphate groups in natural and synthetic oligomers and polymers in the presence of chemically related groups such as carboxylic acid groups. The method is specifically applicable to biological oligomers and polymers, including phosphopeptides, phosphoproteins and phospholipids. In a specific embodiment, selective labeling of phosphate groups in proteins and peptides, for example, facilitates separation, isolation and detection of phosphoproteins and phosphopeptides in complex mixtures of proteins. Selective labeling can be employed to selectively introduce phosphate labels at phosphate groups in an oligomer or polymer, e.g., in a peptide or protein. Detection of the presence of the label, is used to detect the presence of the phosphate group in the oligomer or polymer. The method is useful for the detection of phosphoproteins or phosphopeptides.Type: GrantFiled: June 12, 2001Date of Patent: May 30, 2006Assignee: University of WashingtonInventors: Ruedi Aebersold, Huilin Zhou
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Patent number: RE39664Abstract: The present invention relates to improved specific binding assay devices comprising a chromatographic medium including a reaction site at which a specific binding reagent is immobilized, a sample application well located adjacent to the chromatographic medium and offset upstream from the reaction site, and liquid absorption blotter offset downstream from the reaction site.Type: GrantFiled: November 21, 1995Date of Patent: May 29, 2007Assignee: Inverness Medical Switzerland GmbHInventors: Julian Gordon, Charles Stuart Gredell Pugh
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Patent number: RE39816Abstract: A diagnostic tool is disclosed for accurately and rapidly diagnosing the condition of an ailing organ. Although applicable to numerous organ and organ systems, this application particularly illustrates the concept of conjunctive marker utilization as it relates to diagnosing and distinguishing congestive heart failure. The invention particularly relates to the conjunctive utilization of cardiac Troponin I (cTn-I) and natriuretic peptide, e.g. ANP, pro-ANP, BNP, pro-BNP and CNP as a retrospective tool for diagnosing the underlying mechanism of heart failure and as a prospective analytical device for monitoring disease progression and efficacy of therapeutic agents.Type: GrantFiled: April 29, 2005Date of Patent: September 4, 2007Assignee: Nanogen Inc.Inventors: Eric B. Stanton, George Jackowski