Carrier Is Water Suspendible Particles Patents (Class 436/534)
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Patent number: 9939433Abstract: Latex particles for a particle agglutination assay, with which it is possible to carry out high-sensitivity assay while highly suppressing a non-specific reaction are disclosed. The latex particles being obtained by emulsion polymerization with a polymerizable monomer having a phenyl group, and a polymerizable monomer having a phenyl group and a sulfonate in an aqueous medium including a nonionic surfactant at a concentration of 0.005 to 0.02 wt % based on the aqueous medium, the latex particles having an average particle size of 0.005 to 1.0 ?m.Type: GrantFiled: December 28, 2012Date of Patent: April 10, 2018Assignee: SEKISUI MEDICAL CO., LTD.Inventors: Shinichiro Kitahara, Yuki Takahashi
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Patent number: 9568471Abstract: This invention relates to assays for a Plasmodium analyte in a liquid sample such as a body fluid. More particularly, the invention relates to a method and apparatus for the detection of a ligand in a body fluid such as urine or blood, which can diagnose malarial infection.Type: GrantFiled: January 25, 2006Date of Patent: February 14, 2017Assignee: The Johns Hopkins UniversityInventors: David J. Sullivan, Peter Scholl
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Patent number: 9447232Abstract: A process for producing carrier polymer particles comprising covering the surface of organic polymer particles having a particle diameter of 0.1 to 20 micrometers with a saccharide by chemically bonding the organic polymers and the saccharide.Type: GrantFiled: February 8, 2013Date of Patent: September 20, 2016Assignee: JSR CORPORATIONInventors: Masayuki Takahashi, Tetsuo Fukuta, Kiyoshi Kasai, Toshihiro Ogawa, Satoshi Katayose, Kouji Tamori, Kinji Yamada
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Patent number: 9201059Abstract: The invention relates to a microfluidic system comprising a microfluidic device having a first and a second opposite surfaces and an optical detector, the microfluidic device comprises a flow channel with a detection channel section having a length of at least about 1 mm, the microfluidic device comprises at least one aperture section comprising at least a part of said detection channel section and a transparent window into said detection channel section, the optical detector is arranged to determine at least one optical property of said aperture section as a function of time. The flow channel may have capillary dimensions and/or it may wholly or fully be arranged to drive a fluid flow by applying external forces. The microfluid device may be used to determine various properties of a sample fluid, for example it may be used to determine a samples coagulation properties and/or reactions of one or more components in a fluid sample as a function of time.Type: GrantFiled: March 13, 2009Date of Patent: December 1, 2015Assignee: SCANDINAVIAN MICRO BIODEVICES APSInventors: Niels Kristian Bau-Madsen, Jacques Jonsmann, Bent Overby, Christianus Berendsen
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Patent number: 8993345Abstract: A method of producing functional molecule-containing silica nanoparticles on which a biomolecule is bonded, containing the steps of: allowing silica nanoparticles containing a functional molecule and having a thiol group on a surface thereof to coexist with a linker molecule having a maleimido group and a carboxyl group in an aprotic solvent, thereby allowing formation of a thioether bond between the thiol group and the maleimido group, and obtaining functional molecule-containing silica nanoparticles on which the linker molecule is bonded; and allowing the functional molecule-containing silica nanoparticles on which the linker molecule is bonded to coexist with carbodiimide and a biomolecule having an amino group in an aqueous solvent, thereby allowing formation of an amide bond between the carboxyl group active esterified by the carbodiimide, and the amino group of the biomolecule.Type: GrantFiled: October 25, 2013Date of Patent: March 31, 2015Assignee: Furukawa Electric Co., Ltd.Inventors: Hideki Aizawa, Michio Ohkubo, Masataka Nishida
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Patent number: 8987005Abstract: [PROBLEMS] To provide a reagent for measuring agglutination by using a reaction accelerator, which causes no spontaneous agglutination of receptor-sensitized carrier particles in the coexistence of these carrier particles, and a measurement method. [MEANS FOR SOLVING PROBLEMS] A reagent for measuring agglutination by using a specific amine compound, whereby aggregation based on a specific reaction can be accelerated without causing spontaneous agglutination of carrier particles, and measurement method.Type: GrantFiled: December 27, 2006Date of Patent: March 24, 2015Assignee: Sekisui Medical Co., Ltd.Inventors: Mitsuaki Yamamoto, Tadaaki Yoshida
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Patent number: 8940549Abstract: The present invention aims to provide an assay reagent and an assay for accurately measuring KL-6, in particular, an assay reagent and an assay for accurately measuring KL-6 in samples containing a rheumatoid factor and/or a nonspecific substance other than the rheumatoid factor. KL-6 in samples that contain a rheumatoid factor and/or a nonspecific substance other than the rheumatoid factor can be accurately measured using an immunoassay reagent comprising a solution at a pH of 4.0 to 5.5 containing a rheumatoid factor interference inhibitor and a solution of an insoluble carrier on which anti-KL-6 antibodies are immobilized.Type: GrantFiled: June 30, 2010Date of Patent: January 27, 2015Assignees: Sekisui Medical Co., Ltd., Eidia Co., Ltd.Inventors: Junichi Kondou, Mitsuaki Yamamoto, Chie Kaneko
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Patent number: 8871518Abstract: A monoclonal antibody that does not show a crossreactivity with middle-molecular weight (MMW) adiponectin and specifically reacts with high-molecular weight (HMW) adiponectin alone is disclosed. The monoclonal antibody of the present invention can be produced by using HMW adiponectin as an antigen. According to the monoclonal antibody of the present invention, a convenient, high-accurate, and versatile reagent for analyzing HMW adiponectin can be provided.Type: GrantFiled: May 6, 2010Date of Patent: October 28, 2014Assignees: Otsuka Pharmaceutical Co., Ltd., LSI Medience CorporationInventors: Suguru Akamatsu, Kiyonori Katsuragi, Hideaki Onishi, Midori Abe, Toru Hadama, Ayako Nishimura, Mio Ooguchi
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Patent number: 8865425Abstract: The present invention relates to a reagent for the measurement of FDP comprising a carrier sensitized with at least two monoclonal antibodies selected from three monoclonal antibodies having different reactivity towards FDP. The present invention also relates to a reagent kit comprising the reagent and a method for measurement of FDP using the reagent or reagent kit.Type: GrantFiled: January 29, 2013Date of Patent: October 21, 2014Assignee: Sysmex CorporationInventors: Katsushi Kobayashi, Masumi Murakami, Mayumi Sugimoto
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Patent number: 8865424Abstract: The present invention relates to an anti-FDP monoclonal antibody selected from the first, second and third monoclonal antibodies having different reactivity towards FDP. The present invention also relates to a reagent and reagent kit for the measurement of FDP and a method for measurement of FDP using the anti-FDP monoclonal antibodies.Type: GrantFiled: January 28, 2013Date of Patent: October 21, 2014Assignee: Sysmex CorporationInventors: Naoya Okitsu, Katsushi Kobayashi, Noriaki Nakajima, Masumi Murakami, Kouji Sakaguchi, Ayumi Asaeda, Mayumi Sugimoto
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Patent number: 8759114Abstract: Peptides useful in determining the presence of autoantibodies in patients suffering from rheumatoid arthritis are disclosed.Type: GrantFiled: June 17, 2013Date of Patent: June 24, 2014Assignee: Stichting Voor de Technische WetenschappenInventors: Waltherus Jacobus Wilhelmus Van Venrooij, Jan Wouter Drijfhout, Martinus Adrianus Maria Van Boekel, Gerardus Jozef Maria Pruijn
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Patent number: 8741600Abstract: A method of accurately separating immunoglobulin monomers by subjecting an immunoglobulin solution containing at least immunoglobulin monomers and immunoglobulin aggregates to cross-flow filtration using an ultrafiltration membrane, an ultrafiltration membrane module, and a cross-flow filtration apparatus. The method can separate immunoglobulin monomers by subjecting an immunoglobulin solution containing at least immunoglobulin monomers and immunoglobulin aggregates and having an immunoglobulin concentration of 1 to 150 g/L to cross-flow filtration using an ultrafiltration membrane having a molecular weight cut-off of 100,000 or more and less than 500,000 so that immunoglobulin monomers passes through the ultrafiltration membrane with a permeability of 80% or more while achieving a fractionation performance in which the permeability ratio of immunoglobulin dimers to immunoglobulin monomers that pass through the ultrafiltration membrane is 0.20 or less.Type: GrantFiled: June 18, 2008Date of Patent: June 3, 2014Assignee: Asahi Kasei Kabushiki KaishaInventors: Fumihiko Yamaguchi, Hitoshi Mizomoto, Shinya Hamasaki, Takena Mochizuki
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Patent number: 8716033Abstract: The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.Type: GrantFiled: February 12, 2007Date of Patent: May 6, 2014Assignee: Life Technologies CorporationInventors: Brian Agnew, Schuyler B. Corry, Kyle R. Gee
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Patent number: 8691594Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations.Type: GrantFiled: October 31, 2012Date of Patent: April 8, 2014Assignee: Bioarray Solutions, Ltd.Inventor: Michael Seul
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Patent number: 8685662Abstract: The present invention pertains to a method for in vitro prognosticating and/or diagnosing cerebral cerebral malaria, wherein said method comprises a step of detecting non-erythroid spectrin or fragments thereof, and/or antibodies directed against non-erythroid spectrin, in a biological sample. Reagents and kits for performing this method are also disclosed.Type: GrantFiled: March 30, 2007Date of Patent: April 1, 2014Assignees: Institut Pasteur, Universite Pierre et Marie Curie (Paris 6)Inventors: Sylviane Pied, Vincent Guiyedi, Pierre-André Cazenave, Maryvonne Kombila, Youri Chanseaud
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Patent number: 8580530Abstract: The invention relates to a method for detecting a plurality of antigenic molecules carried by erythrocytes and/or a plurality of anti-erythrocyte antibodies of an individual, comprising bringing a sample into contact with distinguishable beads, on which are attached a) antibodies specific for said antigens, or b) erythrocytes, erythrocyte membrane fragments or blood group antigens.Type: GrantFiled: June 6, 2008Date of Patent: November 12, 2013Assignee: Bio-Rad InnovationsInventors: Frederic Buffiere, Yves Raisin, Eliane Rivalin, Amparo Sanjuan
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Patent number: 8580531Abstract: The invention relates to a method for detecting a plurality of antigenic molecules carried by erythrocytes and/or a plurality of anti-erythrocyte antibodies, said antigenic molecules carried by the erythrocytes consisting of antigenic molecules carried not only by the erythrocytes, but also by at least one other cell population, other than the blood group antigen molecules, said method comprising bringing a sample into contact with distinguishable beads, on which are attached a) antibodies specific for said antigens, or b) erythrocytes or erythrocyte membrane fragment.Type: GrantFiled: June 6, 2008Date of Patent: November 12, 2013Assignee: Bio-Rad InnovationsInventors: Frederic Buffiere, Yves Raisin, Eliane Rivalin, Amparo Sanjuan
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Patent number: 8563329Abstract: Modified branched polymers are combined with bioactive agents which are one member of a binding pair for use in an assay.Type: GrantFiled: May 2, 2006Date of Patent: October 22, 2013Assignee: ANP Technologies, Inc.Inventors: Ray Yin, Dujie Qin, Jing Pan
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Patent number: 8557604Abstract: A lateral flow, membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes phosphorescence to detect the signals generated by excited phosphorescent labels. The labels may have a long emission lifetime so that background interference from many sources, such as scattered light and autofluorescence, is practically eliminated during detection. In addition, the phosphorescent labels may be encapsulated within particles to shield the labels from quenchers, such as oxygen or water, which might disrupt the phosphorescent signal.Type: GrantFiled: December 14, 2007Date of Patent: October 15, 2013Assignee: Kimberly-Clark Worldwide, Inc.Inventor: Xuedong Song
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Patent number: 8551786Abstract: Systems and methods for enhancing fluorescent detection of target molecules in a test sample are for use with an irradiating device. First fluorophores are provided for absorption of EMF radiation, and emission of a first signal. Second fluorophores are provided for partial absorption of the first signal, and emission of a second signal distinguishable from the first signal. The fluorophores are combined with the test sample, and secured to the target molecules and relative to one another. After the first fluorophores receive the EMF radiation from the irradiating device, the first signal is detected, together with the second spectral signal if the target molecules are present in the test sample.Type: GrantFiled: July 9, 2008Date of Patent: October 8, 2013Assignee: FIO CorporationInventors: Warren Che Wor Chan, Travis Leon Jennings, Jesse M. Klostranec
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Patent number: 8546148Abstract: A composition for use as an assay reagent comprises a solid support comprising a member of a signal producing system and a coating of a synthetic copolymer. The synthetic copolymer comprises a first polymerized monomer comprising a pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and a second polymerized monomer comprising a pendant moiety comprising at least 1 carbon atoms and at least 2 heteroatoms. In some embodiments the copolymer comprises a polyethylenic backbone comprising the pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and the pendant moiety comprising at least 1 carbon atom and at least 2 heteroatoms.Type: GrantFiled: April 9, 2012Date of Patent: October 1, 2013Assignee: Siemens Healthcare Diagnostics IncInventors: Bhalchandra Lele, Pratap Singh, Raphael Bartz, Jason A. Kellogg
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Patent number: 8524452Abstract: Disclosed is an agent for inhibiting decrease in measured values in immunoassays, which may reduce the influences by interfering substances in a test sample so as to promote the accuracy of the immunoassays, as well as an immunoassay and a reagent for immunoassays using the same, with which the decrease in the measured values due to the interfering substances is reduced. The agent for inhibiting decrease in measured values in immunoassays, caused by interfering substances, is an ionic surfactant having a molecular weight of 1000 to 100,000, the agent being a polymer in which a hydrophobic cyclic monomer(s) having an ionic functional group(s) is(are) polymerized.Type: GrantFiled: February 25, 2005Date of Patent: September 3, 2013Assignee: Denka Seiken Co., Ltd.Inventors: Yasunori Minakawa, Michie Saito, Hiroshi Matsui
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Patent number: 8518713Abstract: Generally, conjugate systems, self-illuminating quantum dot conjugates, methods of detecting a target in a host, methods of treating a disease in a host, and the like, are described herein.Type: GrantFiled: June 13, 2012Date of Patent: August 27, 2013Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Jianghong Rao, Min-kyung So, Chenjie Xu, Andreas M. Loening, Sanjiv S. Gambhir
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Patent number: 8513024Abstract: Provided is a detection method for a target substance capable of enhancing detection sensitivity and quantitative property of a magnetic biosensor, while keeping monodispersity and dispersion stability of magnetic markers, including the steps of: reacting the target substance in a sample solution with a first target substance trapping member immobilized on a sensing element and with a second target substance trapping member immobilized on a gel particle to hold the gel particle on the sensing element; adjusting a magnetic marker precursor including the gel particle and a magnetic material precursor existing in the gel particle by bringing the magnetic material precursor into contact with the gel particle; synthesizing a magnetic material from the magnetic material precursor held on the gel particle, thereby adjusting the magnetic markers; and detecting the magnetic markers with the sensing element.Type: GrantFiled: February 4, 2009Date of Patent: August 20, 2013Assignee: Canon Kabushiki KaishaInventor: Kazumichi Nakahama
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Patent number: 8497137Abstract: Capture particles for harvesting analytes from solution and methods for using them are described. The capture particles are made up of a polymeric matrix having pore size that allows for the analytes to enter the capture particles. The pore size of the capture particles are changeable upon application of a stimulus to the particles, allowing the pore size of the particles to be changed so that analytes of interest remain sequestered inside the particles. The polymeric matrix of the capture particles are made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. The capture particles may be used to isolate and identify analytes present in a mixture. They may also be used to protect analytes which are typically subject to degradation upon harvesting and to concentrate low an analyte in low abundance in a fluid.Type: GrantFiled: August 19, 2008Date of Patent: July 30, 2013Inventors: Alessandra Luchini, Lance Liotta, Emanuel Petricoin, Barney Bishop, Caterina Longo, Virginia Espina, Alexis Patanarut
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Patent number: 8481326Abstract: A microfluidic device and method for measuring a level of cholesterol therewith are provided. The cholesterol measurement apparatus includes a microfluidic device including a plurality of chambers and at least one channel through which the plurality of chambers are interconnected. The plurality of chambers include a reaction chamber which contains a capture binder, a buffer chamber which contains an elution buffer and is connected to the reaction chamber, and at least one detection chamber which contains a cholesterol measurement reagent and is connected to the reaction chamber.Type: GrantFiled: July 8, 2011Date of Patent: July 9, 2013Assignee: Samsung Electronics Co., Ltd.Inventor: In Wook Kim
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Patent number: 8481332Abstract: A liposomal composition, preferably a vaccine, comprising liposomes formed of liposome forming compounds, containing coentrapped polysaccharide antigen and T-cell dependent protein carrier, such as tetanus toxoid or diphtheria toxin modified to render it non-toxic. The invention is of use in the production of vaccines against Haemophilus influenzae, Streptococcus pneumoniae or Neisseria meningitidis.Type: GrantFiled: January 24, 2011Date of Patent: July 9, 2013Assignee: Stichting Voor de Technische WetenshappenInventors: Waltherus Jacobus Wilhelmus Van Venrooij, Jan Wouter Drijfhout, Martinus Adrianus Maria Van Boekel, Gerardus Jozef Maria Pruijn
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Patent number: 8456158Abstract: A magnetic binding substance, which is a first binding substance that specifically binds with a target substance, having magnet enveloping dielectric particles, which have magnetic particles enveloped therein and surfaces modified with functional groups that exhibit polarity within a liquid sample, attached thereto, and a labeling binding substance, which is a second binding substance that specifically binds with the target substance having photoresponsive labels attached thereto, are mixed with the liquid sample such that binding reactions occur. A magnetic field is generated within a sample cell, to draw the magnetic binding substance to a local region. Excitation light is irradiated only onto a predetermined region including the local region while the magnetic binding substance is drawn to the local region, causing the photoresponsive labels present therein to generate optical signals. The optical signals are detected.Type: GrantFiled: July 28, 2010Date of Patent: June 4, 2013Assignee: FUJIFILM CorporationInventor: Hisashi Ohtsuka
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Patent number: 8431415Abstract: The present invention provides a reagent for an immunoassay comprising insoluble carrier particles which can give the values to be determined with high accuracy and reliability, and can be stored for a long time; an immunoassay using the reagent; and a method for keeping the reagent stable.Type: GrantFiled: July 27, 2009Date of Patent: April 30, 2013Assignee: TFB, Inc.Inventor: Kayoko Shigenobu
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Patent number: 8394647Abstract: Methods and reagents are disclosed for reducing an amount of non-covalently bound polysaccharide on a support. The method comprises contacting a support comprising both covalently bound polysaccharide and non-covalently bound polysaccharide with an aqueous solution comprising an amount of a chaotropic agent effective to remove non-covalently bound polysaccharide from the support.Type: GrantFiled: February 17, 2011Date of Patent: March 12, 2013Assignee: Siemens Healthcare Diagnostics Inc.Inventors: Bhalchandra Lele, Pratap Singh, Asoke Chatterjee
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Patent number: 8383424Abstract: Methods and reagents are disclosed for determining the presence and/or amount of cyclosporin A in a medium suspected of containing cyclosporin A. In the method a combination is provided in a medium. The combination comprises (i) the sample, (ii) a first member of a signal producing system (sps) associated with a first support wherein the first sps member is capable of activating a second member of the sps and wherein the first support is associated with a first member of a specific binding pair, and (iii) the second sps member associated with a second support wherein the second sps member is activatable by the first sps member. The second support comprises either (I) cyclosporin C or cyclosporin A and the combination further comprises a conjugate of an antibody for cyclosporin A and a second member of the specific binding pair or (II) antibody for cyclosporin A and the combination further comprises a conjugate of cyclosporin A and a second member of the specific binding pair.Type: GrantFiled: April 27, 2011Date of Patent: February 26, 2013Assignee: Siemens Healthcare DiagnosticsInventors: Yi Feng Zheng, Deb K. Vickery, Susan C. Swann
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Patent number: 8354235Abstract: The invention concerns a method for increasing the dynamic measuring range of especially immunological test elements in particular immunological chromatography test strips that can be evaluated optically that are based on specific binding reactions. The invention enables the dynamic measuring range of test elements based on specific binding reagents, especially of immunological test elements to be shifted towards higher analyte concentrations without impairing the lower detection limit.Type: GrantFiled: November 7, 2006Date of Patent: January 15, 2013Assignee: Roche Diagnostics Operations, Inc.Inventors: Ludger Bodenbach, Juergen Schaeffler, Juergen Spinke
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Patent number: 8318492Abstract: The present invention relates in one aspect to a method for determining the cell culture history of a cell unit labelled with more than one type of tag comprising the steps of: (a) measuring one or more parameters of each tag that is used to label the cell unit; (b) identifying each tag in the cell unit; and (c) correlating the identity of each tag to the identity of the cell unit and/or the specific cell culture conditions to which the cell unit has been exposed.Type: GrantFiled: February 25, 2008Date of Patent: November 27, 2012Assignee: Plasticell LimitedInventors: Yen Choo, Fraser Hornby, John Girdlestone
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Patent number: 8309368Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relics on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations.Type: GrantFiled: June 11, 2007Date of Patent: November 13, 2012Assignee: BioArray Solutions, Ltd.Inventor: Michael Seul
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Patent number: 8263417Abstract: Generally, conjugate systems, self-illuminating quantum dot conjugates, methods of detecting a target in a host, methods of treating a disease in a host, and the like, are described herein.Type: GrantFiled: January 4, 2007Date of Patent: September 11, 2012Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Jianghong Rao, Min-kyung So, Chenjie Xu, Andreas M. Loening, Sanjiv S. Gambhir
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Patent number: 8252569Abstract: A microfluidic device for the concentration and lysis of cells or viruses and a method of concentrating and lysing cells or viruses using the microfluidic device include: magnetic beads, a reaction chamber in which the magnetic beads are accommodated and a laser source. The reaction chamber includes a plurality of electrodes which cross each other and are separated by a dielectric to generate an electric field and a vibrating part agitating the magnetic beads in the chamber. The laser source radiates a laser onto the magnetic beads in the reaction chamber.Type: GrantFiled: February 25, 2011Date of Patent: August 28, 2012Assignee: Samsung Electronics Co., Ltd.Inventors: Yoon-kyoung Cho, Jeong-gun Lee, Sung-young Jeong
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Patent number: 8227263Abstract: A process for producing polymer particles having an antigen or an antibody introduced into the surface thereof, by carrying out miniemulsion polymerization using a monomer, a radical polymerization initiator, an emulsifier, and a hydrophobe in the presence of the antigen or antibody to thereby produce the polymer particles, is disclosed. According to the process, it is possible to provide a reagent for immunological analysis having an excellent detection sensitivity and capable of avoiding a nonspecific reaction which occurs in conventional methods.Type: GrantFiled: October 16, 2007Date of Patent: July 24, 2012Assignees: Mitsubishi Chemical Medience Corporation, National University Corporation Chiba UniversityInventors: Tatsuo Taniguchi, Akihiro Mizuno, Tokio Sawai, Hiroyuki Tsubota
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Patent number: 8182746Abstract: An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries and includes a valve system. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective intersections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes. The apparatus may also include a plurality of valves surrounding each of the analyte concentrators to localize each of the concentrators to improve the binding of one or more analytes of interest.Type: GrantFiled: October 31, 2007Date of Patent: May 22, 2012Assignee: Princeton Biochemicals, Inc.Inventor: Norberto A. Guzman
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Patent number: 8153442Abstract: Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with a solid support such as, for example, a particle. The support includes a chemiluminescent composition that includes a metal chelate. The present inventors observed that, when such support such as, e.g., particles, were employed in assays for the determination of an analyte, stability of signal output by the chemiluminescent composition associated with the particle was unacceptably reduced as compared to particles including other chemiluminescent compositions. In accordance with embodiments of the present invention, the stability of signal output from such particles is enhanced by including in a medium that contains the particles a sufficient amount of one or more stabilizing agents, which may be a chelating agent and/or a metal chelate such as, for example, the metal chelate that is associated with the particle.Type: GrantFiled: October 21, 2009Date of Patent: April 10, 2012Assignee: Siemens Healthcare Diagnostics Inc.Inventors: Alan R. Craig, Zhu Teng, Carsten Schelp, Jason Snyder, Christine Moran
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Patent number: 8137985Abstract: A flow-through assay for detecting the quantity of an analyte residing in a test sample is provided. The flow-through assay contains a porous membrane that is in fluid communication with probe conjugates that contain a specific binding member and a detectable probe. The porous membrane also defines a detection zone and a calibration zone. The calibration zone contains a polyelectrolyte substantially non-diffusively immobilized on the porous membrane. The polyelectrolyte is capable of generating a detectable calibration signal that can be readily compared (visually, quantitatively, and the like) to a detection signal to determine the amount of analyte in the test sample.Type: GrantFiled: November 12, 2009Date of Patent: March 20, 2012Assignee: Kimberly-Clark Worldwide, Inc.Inventors: Xuedong Song, Ning Wei, Curtis N. Sayre
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Patent number: 8110407Abstract: Disclosed is a fluorescent semiconductor microparticle assembly comprising at least three kinds of fluorescent semiconductor microparticles with an average particle size of from 1 to 10 nm, having the same chemical composition, a different average particle size and a different emission maximum wavelength in the emission spectra, wherein a standard deviation of emission intensity in each of the at least three kinds of fluorescent semiconductor microparticles is not more than 15%.Type: GrantFiled: August 22, 2007Date of Patent: February 7, 2012Assignee: Konica Minolta Medical & Graphic, Inc.Inventors: Kazuya Tsukada, Hisatake Okada, Hideki Hoshino
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Patent number: 8101429Abstract: This invention is directed to a lateral flow assay for detecting the presence of an analyte in a liquid test sample. The lateral flow assay represents an improvement in the ability to accurately and with high fidelity to detect the presence or absence of a target analyte in a liquid sample, in part, by encompassing a reference region of immobilized, non-diffusible analyte that allows for detection of any factors that interfere with the interaction and binding of the analyte to the labeled capture reagent. Any influences on the interaction and binding of the analyte that is free in solution in the liquid test sample to its complementary labeled reagent will be encountered in parallel in the binding between the immobilized analyte in reference region to the labeled reagent as it diffuses through the reference region. In one embodiment, the lateral flow assay of the invention is a urine-based human Chorionic Gonadotropin (hCG) assay.Type: GrantFiled: June 3, 2004Date of Patent: January 24, 2012Assignee: Siemens Healthcare Diagnostics Inc.Inventors: Gary H. Krauth, David J. Ledden
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Patent number: 8030092Abstract: An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries and includes a valve system. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective intersections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes. The apparatus may also include a plurality of valves surrounding each of the analyte concentrators to localize each of the concentrators to improve the binding of one or more analytes of interest.Type: GrantFiled: November 17, 2006Date of Patent: October 4, 2011Assignee: Princeton Biochemicals, Inc.Inventor: Norberto A. Guzman
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Patent number: 8026107Abstract: Provided are an agglutination-inhibition assay and a reagent for agglutination-inhibition assay, which can be used for measuring a ligand in a sample at high sensitivity in a wide range from the low-concentration range to the high-concentration range and have good reproducibility of measurement. Specifically, provided are an agglutination-inhibition assay and a reagent for agglutination-inhibition assay, in which used are an insoluble carrier particle carrying a ligand, a specific receptor in the free-form and an insoluble carrier particle carrying a specific receptor which binds to a different site on the ligand than the receptor in the free-form.Type: GrantFiled: July 23, 2007Date of Patent: September 27, 2011Assignee: Sekisui Medical Co., Ltd.Inventors: Tadaaki Yoshida, Hiroshi Takahashi
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Patent number: 7968347Abstract: An example method for detecting an analyte in a sample of a bodily fluid comprises the steps of exposing the bodily fluid sample to electromagnetic energy to cause a thermoelastic expansion in the analyte, and detecting a photoacoustic signal in the sample that results from the thermoelastic expansion.Type: GrantFiled: July 11, 2007Date of Patent: June 28, 2011Assignee: The Curators of the University of MissouriInventors: John A. Viator, Paul S. Dale, Ryan M. Weight, Peter Sutovsky
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Patent number: 7897328Abstract: The present invention provides compositions and methods for treating or preventing antibody mediated graft rejection and blood typing.Type: GrantFiled: March 23, 2007Date of Patent: March 1, 2011Assignee: AbSorber ABInventors: Jan Holgersson, Jining Liu, Linda Lindberg, Per Grufman
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Patent number: 7893177Abstract: The invention provides nanoparticles and nanoparticle conjugates comprising one or more redox-active species, methods of making nanoparticles and nanoparticle conjugates, and methods for using nanoparticles and nanoparticle conjugates, for example, as diagnostic agents for the detection of various analytes.Type: GrantFiled: October 31, 2007Date of Patent: February 22, 2011Assignee: Iowa State University Research Foundation, Inc.Inventors: Marc D. Porter, Jennifer H. Granger
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Patent number: 7892762Abstract: A method for aiding in differentiating irritable bowel syndrome from inflammatory bowel disease by determining the level of total endogenous human lactoferrin in clinical specimens, such as feces, mucus and bile, wherein an elevated level of lactoferrin substantially precludes diagnoses of IBS and other noninflammatory etiologies, and a kit usable in such method are provided. Further provided is a method for quantitating the level of total endogenous human lactoferrin in clinical specimens, such as feces, mucus and bile, to monitor gastrointestinal inflammation in persons having inflammatory bowel disease.Type: GrantFiled: June 17, 2009Date of Patent: February 22, 2011Assignee: Techlab, Inc.Inventors: James Hunter Boone, David Maxwell Lyerly, Tracy Dale Wilkins, Richard Littleton Guerrant
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Patent number: 7888133Abstract: The invention relates to method of detecting autoantibodies from patients suffering from rheumatoid arthritis. To this end, according to the invention, at least two peptide units are used of which at least one peptide unit comprises a part not derived from (pro)filaggrin, fibrin, fibrinogen, vimentin, cytokeratin 1 and cytokeratin 9, and which peptide unit comprises the motif XG, and a peptide unit comprising the motif XnonG, wherein X is a citrulline or an analogue thereof, and nonG is an amino acid other than glycine.Type: GrantFiled: December 11, 2002Date of Patent: February 15, 2011Assignee: Stichting Voor De Technische WetenschappenInventors: Waltherus Jacobus Wilhelmus Van Venrooij, Jan Wouter Drijfhout, Martinus Adrianus Maria Van Boekel, Gerardus Jozef Maria Pruijn
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Patent number: RE43979Abstract: The invention relates to a method for detecting specific target-cells in a simple and time saving way, using paramagnetic particles, antibodies recognizing the Fc portions of target-cell associating antibodies and target-cell associating antibodies directed to specific antigen determinants in the target-cell membranes. Incubation of the cell suspension with a mild detergent and/or second set of antibodies or antibody fragments, prelabeled or not with fluorescent agents, metallocolloids, radioisotopes, biotincomplexes or certain enzymes allowing visualization, with dramatically increase the specificity of the method. The method can further be used for isolation of the target-cells by magnetic field application and kit for performing the method according to the invention is described.Type: GrantFiled: February 5, 2003Date of Patent: February 5, 2013Assignee: Abbott LaboratoriesInventors: Oystein Fodstad, Gunnar Kvalheim