Antigen Or Antibody Entrapped Within The Carrier (e.g., Gel, Hollow Fiber, Etc.) Patents (Class 436/535)
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Patent number: 11614444Abstract: A time-resolved fluorescence immunochromatography test paper card for detecting butralin, which comprises a cover body and a housing body, wherein the cover body is provided with a test hole, a loading hole and a through-hole, an isolating mechanism is arranged in the test hole and the loading hole, the isolating mechanism comprises a first isolating ring and a second isolating ring, the top lateral walls of which are respectively provided with a first lug boss and a second lug boss, one end of the upper surface of the working board is concave towards the inner of the working board to provide a groove, there is a nitrocellulose membrane, a binding pad, a sample pad and a mark zone successively provided between the water absorbing block and the other end of the working board, and the lateral wall at one end of the working board is provided with a bump.Type: GrantFiled: July 27, 2020Date of Patent: March 28, 2023Assignee: TOBACCO RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Xiuguo Wang, Xiao Zheng, Yalei Liu, Kuan Fang, Xiaolian Zhang
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Patent number: 11525826Abstract: The present invention relates to the field of biological diagnosis in oncology. It relates to a method and apparatus for detecting and/or characterizing tumor cells by detecting one or more elements of the tumor cell secretome, in particular one or more peptides or proteins, and, in particular, one or more tumor markers. The invention also relates to detecting and/or characterizing droplets of tumor cells and their method of preparation.Type: GrantFiled: April 18, 2017Date of Patent: December 13, 2022Assignees: Chu Montpellier, Ecole Superieure De Physique Et De Chimie Industrielles De La Ville De Paris, Centre National De La Recherche Scientifique, Université De Montpellier, Axlr, Satt Du Languedoc RoussillonInventors: Catherine Michèle Marilou Alix-Panabieres, Andrew David Griffiths, Raphaël Clément Li-Ming Doineau, Clément Nizak, Philippe Chi-Thanh Nghe, Jean-Marie Pierre Baudry, Elodie Michèle Christine Brient-Litzler, Klaus Eyer, Jérôme Bibette
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Patent number: 9505977Abstract: A gadolinium (Gd) loaded scintillation gel (Gd-ScintGel) compound allows for neutron and gamma-ray detection. The unique gel scintillator encompasses some of the best features of both liquid and solid scintillators, yet without many of the disadvantages associated therewith. Preferably, the gel scintillator is a water soluble Gd-DTPA compound and water soluble fluorophores such as: CdSe/ZnS (or ZnS) quantum dot (Q-dot) nanoparticles, coumarin derivatives 7-hydroxy-4-methylcoumarin, 7-hydroxy-4-methylcoumarin-3-acetic acid, 7-hydroxycoumarin-3-carboxylic acid, and Alexa Fluor 350 as well as a carbostyril compound, carbostyril 124 in a stable water-based gel, such as methylcellulose or polyacrylamide polymers. The Gd-loaded ScintGel allows for a homogenious distribution of the Gd-DTPA and the fluorophores, and yields clean fluorescent emission peaks. A moderator, such as deuterium or a water-based clear polymer, can be incorporated in the Gd-ScintGel.Type: GrantFiled: July 30, 2014Date of Patent: November 29, 2016Assignee: The United States of America Department of EnergyInventors: Catherine Lynn Riddle, Douglas William Akers, Ricky Lynn Demmer, Patricia Denise Paviet, Mark William Drigert
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Patent number: 9023660Abstract: Methods are disclosed for producing a bioweapon-sensitive fibrous-network product, wherein the subject products exhibit a color change in response to exposure to a biological agent (or portion thereof) as used in a biological weapon. Also disclosed are fibrous-network products that contain units of biopolymeric material that impart a color change to the products in response to exposure to a biological agent (or portion thereof) as used in a biological weapon.Type: GrantFiled: January 28, 2013Date of Patent: May 5, 2015Inventors: Dennis Farwell, Keith Baumann
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Patent number: 9018019Abstract: The conductive polymer films of this disclosure reversibly and selectively mediate ligand-receptor interactions. This electrochemical manipulation of biochemical interactions is accomplished by embedding or adsorbing receptors for ligands of interest in or onto a conductive polymer matrix. The matrix can also be doped, for example, with desired ions, polyions, or surfactants. Depending on the receptor properties and dopants utilized, ligand-receptor interactions at the polymer-electrolyte interface are manipulated by controlling the oxidation and reduction of the conductive polymer. The intrinsic charge transfer characteristics of conductive polymers are used to modulate ligand-receptor interactions.Type: GrantFiled: October 4, 2007Date of Patent: April 28, 2015Assignee: President and Fellows of Harvard CollegeInventors: Kevin Kit Parker, Megan O'Grady
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Patent number: 9012240Abstract: An immunoassay device incorporating porous polymeric capture nanoparticles within either the sample collection vessel or pre-impregnated into a porous substratum within fluid flow path of the analytical device is presented. This incorporation of capture particles within the immunoassay device improves sensitivity while removing the requirement for pre-processing of samples prior to loading the immunoassay device. A preferred embodiment is coreshell bait containing capture nanoparticles which perform three functions in one step, in solution: a) molecular size sieving, b) target analyte sequestration and concentration, and c) protection from degradation. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle.Type: GrantFiled: August 26, 2009Date of Patent: April 21, 2015Assignee: George Mason Research Foundation, Inc.Inventors: Lance A. Liotta, Alessandra Luchini, Emanuel F. Petricoin, Virginia Espina
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Patent number: 8993235Abstract: The present invention contemplates use of encapsulated aqueous and non-aqueous reagents, solutions and solvents and their use in laboratory procedures. These encapsulated aqueous or non-aqueous reagents, solutions and solvents can be completely contained or encapsulated in microcapsules or nanocapsules that can be added to an aqueous or non-aqueous carrier solution or liquid required for medical and research laboratory testing of biological or non-biological specimens.Type: GrantFiled: July 21, 2014Date of Patent: March 31, 2015Inventor: Lee H. Angros
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Patent number: 8956880Abstract: The present invention concerns methods for detecting the presence of a micro-organism in a fluid, gaseous and solid samples, together with apparatus for use in same. The invention comprises the use of a plurality of hollow fibers to filter the fluid and the capture of the micro-organisms on the membrane by means of a specific binding pair.Type: GrantFiled: May 11, 2006Date of Patent: February 17, 2015Assignee: MICAP PLCInventors: Elizabeth Ann Keenan, Michael S. Brennand
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Patent number: 8916389Abstract: An object of the present invention is to provide an element for SPR measurement without the need of a mechanical solution-sending mechanism.Type: GrantFiled: June 26, 2007Date of Patent: December 23, 2014Assignee: Nippon Telegraph and Telephone CorporationInventors: Yuzuru Iwasaki, Michiko Seyama, Toru Miura, Tsutomu Horiuchi, Tsuneyuki Haga, Serge Camou
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Patent number: 8841082Abstract: The invention relates to a method for determining one or more cellularly bound analytes in a liquid sample, said method being carried out using a device comprising: at least one feeding zone (5) for applying the liquid sample; a porous membrane (2) that is suitable for letting cellular components penetrate therethrough and includes at least one indicator zone on the membrane, said indicator zone being able to interact with the cellularly bound analyte and containing at least one binding element against the cellularly bound analyte; and at least one absorption area (3) on the membrane, which absorbs the liquid after the liquid has passed the indicator zones. The at least one indicator zone lies between the feeding zone (5) and the absorption area (3).Type: GrantFiled: December 14, 2007Date of Patent: September 23, 2014Assignee: Medion Diagnostics AGInventors: Peter Schwind, Iwan Aebischer
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Patent number: 8802449Abstract: An objective of the present invention is to provide immunoassay microchips in which microstructures of beads having a sufficient reaction area were constructed within microchannels while suppressing flow path resistance, and to provide simple and highly-sensitive immunoassay methods for microsamples. The objective was achieved by immunoassay microchips comprising microchannels with microstructures arranged in at least a portion of the microchannels, the microstructures retaining microbeads uniformly dispersed in photo-cured hydrophilic resins, and the microbeads having a primary antibody immobilized on their surfaces, and by immunoassay methods using the microchips.Type: GrantFiled: December 25, 2006Date of Patent: August 12, 2014Assignees: Institute of Microchemical Technology, Kansai Paint Co., Ltd.Inventors: Masaya Kakuta, Manabu Tokeshi, Hitoshi Izumida, Takahide Takadera, Kenji Miyagawa
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Patent number: 8790882Abstract: The present invention relates to the field of immunology and hyperproliferative diseases. More specifically, the present invention relates to a method of detecting and monitoring therapeutic antibody:antigen complex, soluble antigen and soluble therapeutic antibody, wherein a patient has undergone at least one course of immunotherapy. Yet further, levels of therapeutic antibody:antigen complexes, soluble antigens or soluble therapeutic antibodies may be measured and used to stage or monitor a hyperproliferative disease.Type: GrantFiled: July 3, 2013Date of Patent: July 29, 2014Assignee: Board of Regents, The University of Texas SystemInventors: Maher Albitar, Michael J. Keating, Taghi Manshouri
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Patent number: 8785124Abstract: The present invention contemplates use of encapsulated aqueous and non-aqueous reagents, solutions and solvents and their use in laboratory procedures. These encapsulated aqueous or non-aqueous reagents, solutions and solvents can be completely contained or encapsulated in microcapsules or nanocapsules that can be added to an aqueous or non-aqueous carrier solution or liquid required for medical and research laboratory testing of biological or non-biological specimens.Type: GrantFiled: July 17, 2013Date of Patent: July 22, 2014Inventor: Lee H. Angros
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Patent number: 8697111Abstract: An osteochondral plug includes a first scaffold and a second scaffold. The first scaffold may be a solid scaffold containing one or more pendant reactive functional groups. The second scaffold capable of reacting with the one or more pendant reactive functional groups of the first scaffold.Type: GrantFiled: May 12, 2010Date of Patent: April 15, 2014Assignee: Covidien LPInventors: Arpan Desai, Timothy Sargeant, Atu Agawu, Joshua Stopek
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Patent number: 8679808Abstract: The present invention discloses a method for fabricating aerogels, a method for fabricating surface-modified aerogels, and a method for fabricating biocomposites. Take the fabricating method of biocomposites for example, first, a precursor solution is provided and the precursor solution comprises a hydrophilic ionic liquid, a catalyzed hydrolysis and/or condensation reagent, at least one biomolecule. Next, a curing process is performed for the precursor solution to hydrolyze and polymerize the at least one alkoxide monomer and/or aryloxide monomer to wrap at least one biomolecule and thus form biocomposite. Afterwards, an extracting process is performed by a solvent for the biocomposite to substitute the ionic liquid in the biocomposite. Finally, a drying process for the biocomposite is carried out after the extracting process so as to remove the solvent in the biocomposite. Therefore, the biocomposite is formed.Type: GrantFiled: November 17, 2010Date of Patent: March 25, 2014Assignee: Chung Yuan Christian UniversityInventors: Yui-Whei Chen-Yang, Yen-Kuang Li, Ching-Yao Yuan, Tzong-Yuan Wu
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Patent number: 8664001Abstract: An immunochemical filter device which has filter material attached to a support member (such as a cap) which is, in turn, attachable to a sample collection vessel. The filter material includes a labeled binding reagent that is released from the filter material into solution by migration of a liquid sample solution through the filter material. The mixture of the sample solution and the labeled specific binding reagent is transferred to an analyzer device for performance of a method for determining the presence or absence of an analyte in a sample solution.Type: GrantFiled: September 29, 2004Date of Patent: March 4, 2014Assignee: Ani Biotech OyInventors: Aimo Niskanen, Mika Saramaki
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Patent number: 8609438Abstract: In one example embodiment, a protein-immobilized electrode is stably used for long time. In one example embodiment, a method of manufacturing the protein-immobilized electrode includes immobilizing cytochrome c552 having high stability to a chemically-stable gold electrode while maintaining electron transfer capability of the cytochrome c552. In one example embodiment, a self-assembled monolayer is formed on a gold electrode by using hydrophobic thiol and hydrophilic thiol. By dipping the gold electrode on which the self-assembled monolayer is formed in a cytochrome c552 solution, a protein-immobilized electrode in which a cytochrome c552 is immobilized to the gold electrode with the self-assembled monolayer in between is produced.Type: GrantFiled: December 10, 2009Date of Patent: December 17, 2013Assignee: Sony CorporationInventors: Seiji Yamada, Yoshio Goto, Yuichi Tokita
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Patent number: 8574858Abstract: The present disclosure provides immunoassays and kits for detection or quantification of an analyte of interest in a test sample that potentially contains endogenously produced autoantibodies reactive with the analyte.Type: GrantFiled: October 2, 2012Date of Patent: November 5, 2013Assignee: Abbott LaboratoriesInventors: Maciej Adamczyk, Roy Jeffrey Brashear, Phillip G. Mattingly
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Patent number: 8568993Abstract: A diagnostic method for determining the absence or presence of a disease is provided. The method includes assaying the amount and/or types of glycopeptides in a sample from a subject, and comparing these to the amount and types of reference glycopeptides. The method may include the use of a stable isotope label, affinity selection, immunoaffinity chromatography, and glycoproteomics techniques, to identify and quantify changes in glycosylated peptides or glycosylated proteins associated with cancers such as malignant lymphoma or breast cancer, to monitor patient's response to therapy, and to monitor disease recurrence.Type: GrantFiled: June 30, 2008Date of Patent: October 29, 2013Assignee: Purdue Research FoundationInventors: Fred E. Regnier, Stephen B. Hooser, Christina R. Wilson, Wonryeon Cho
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Patent number: 8563260Abstract: A capillary for an immunoassay is provided which comprises an insoluble layer of an oxidase formed on an inner wall surface of said capillary, said oxidase being conjugated to a first antibody, and a layer of a hydrophilic polymer formed on said insoluble layer, said hydrophilic polymer layer containing a second antibody conjugated to a peroxidase, wherein said first and second antibodies are capable of binding to the same antigen.Type: GrantFiled: December 7, 2009Date of Patent: October 22, 2013Assignee: Osaka Prefecture University Publication CorpoarationInventors: Hideaki Hisamoto, Terence Gaba Henares
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Patent number: 8557609Abstract: A method employing gel electrophoresis and optical imaging techniques to measure the amount of biomaterial that attaches to specified locations on a detector slide such as a bioarray or biochip.Type: GrantFiled: October 18, 2010Date of Patent: October 15, 2013Assignee: Maven Biotechnologies, LLCInventors: Shane Dultz, David Ralin, William Rassman
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Patent number: 8506813Abstract: This invention concerns a method, devices, instrument and program for extraction an ingredient from a liquid sample by bidirectional transfer of an aliquot of fluid between compartments, the method can be applied to a wide variety of laboratory techniques such as; solid phase extraction by filter disc, column chromatography, magnetic separation, diagnostic tests and others, the system is suitable for single or multi sample handling, manual operation or integrated into an automated system, can be used in a lab or in field.Type: GrantFiled: June 17, 2008Date of Patent: August 13, 2013Inventor: Beno Alspektor
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Patent number: 8497137Abstract: Capture particles for harvesting analytes from solution and methods for using them are described. The capture particles are made up of a polymeric matrix having pore size that allows for the analytes to enter the capture particles. The pore size of the capture particles are changeable upon application of a stimulus to the particles, allowing the pore size of the particles to be changed so that analytes of interest remain sequestered inside the particles. The polymeric matrix of the capture particles are made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. The capture particles may be used to isolate and identify analytes present in a mixture. They may also be used to protect analytes which are typically subject to degradation upon harvesting and to concentrate low an analyte in low abundance in a fluid.Type: GrantFiled: August 19, 2008Date of Patent: July 30, 2013Inventors: Alessandra Luchini, Lance Liotta, Emanuel Petricoin, Barney Bishop, Caterina Longo, Virginia Espina, Alexis Patanarut
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Patent number: 8497138Abstract: In one aspect the present invention provides a method for selecting a cell or cell colony which produces a polypeptide of interest, comprising a) providing a medium comprising cells and a detection agent, wherein the detection agent is associated with a detectable signal and the detection agent is capable of binding to the polypeptide of interest; b) providing a solid phase having a capture agent disposed thereon, wherein the capture agent is capable of binding to the polypeptide of interest; c) contacting the medium with the solid phase; d) detecting the signal associated with the detection agent; and e) selecting a cell or cell colony associated with the signal, wherein presence of the signal is indicative of a cell or cell colony which produces the polypeptide of interest.Type: GrantFiled: September 30, 2010Date of Patent: July 30, 2013Assignee: Genetix LimitedInventors: Julian Burke, Alasdair Robertson, Xiao Zhang
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Patent number: 8440470Abstract: The disclosure relates to a fabrication process of a biosensor on a semiconductor wafer, comprising steps of: making a central photosensitive zone comprising at least one pixel-type biological analysis device comprising a photosensitive layer, and a first peripheral zone surrounding the central photosensitive zone, comprising electronic circuits. The first peripheral zone is covered by a hydrophilic coating, and the central photosensitive zone is covered with a hydrophobic coating. A barrier of a bio-compatible resin is formed on the second peripheral zone.Type: GrantFiled: December 16, 2010Date of Patent: May 14, 2013Assignees: STMicroelectronics (R&D) Limited, Universite Paul Cezanne Aix Marseille IIIInventors: Jeffrey M. Raynor, Michaël Maurin, Mitchel O'Neal Perley, Pierre-Francois Lenne, Herve Rigneault, Renaud Vincentelli
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Patent number: 8431405Abstract: The present invention related to a pregnancy test device that can selectively detect hyperglycosylated human chorionic gonadotropin (hCG-H) in a liquid sample. The sample can be deposited on a proximal portion of the device for transport to a distal portion of the device. The device can include a release medium formed of a first material and including a detectable label thereon and a capture medium, including a capture site, in fluid communication with the release medium and formed of a second, different material. At least one of the release medium and the capture medium includes a binding member that exhibits a moderate to high affinity for hCG-H and is selectively or preferentially reactive with hCG-H.Type: GrantFiled: August 22, 2012Date of Patent: April 30, 2013Assignee: Church & Dwight Co., Inc.Inventors: Albert R. Nazareth, Timothy Snowden
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Patent number: 8415173Abstract: A gel microdrop composition is provided. In certain embodiments, the gel microdrop composition contains a polymer matrix, an effector particle that releases an effector molecule into the polymer matrix, a first reporter particle that emits a first optically detectable signal and a second reporter particle that emits a second optically detectable signal that is distinguishable from the first optically detectable signal, where the effector particle and said first and second reporter particles are encapsulated by the polymer matrix. Methods of screening that employ the gel microdrop composition and methods of making the gel microdrop composition are also disclosed.Type: GrantFiled: August 26, 2011Date of Patent: April 9, 2013Assignee: Crystal Bioscience Inc.Inventor: William Don Harriman
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Patent number: 8404495Abstract: In one aspect, the present invention is a system, a device and a method for sensing the concentration of an analyte in a fluid or matrix. The analyte may be glucose or any other chemical of interest. The fluid or matrix may be, for example, the fluid in a bioreactor, a food or agricultural product, any fluid or matrix in the body of an animal, or any other fluid or matrix whose concentration of an analyte is under investigation. In one embodiment, the analyte sensing device includes a housing having an interior space. Contained within the housing and in the interior space is one or more analyte sensing component(s). The analyte sensing component, in one embodiment, includes one or more radiation converting element(s), for example, converting chromophores. The radiation converting element(s) are capable of converting or modifying radiation of one or more wavelengths into radiation of one or more different wavelengths.Type: GrantFiled: January 22, 2007Date of Patent: March 26, 2013Assignee: BioTex, Inc.Inventors: Ralph Ballerstadt, Roger McNichols, Ashok Gowda
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Patent number: 8399262Abstract: Disclosed herein are biosensors for the detection of airborne biomolecules. The biosensors include a housing, a sensing component, and optionally a sample capture component. The biosensors may utilize a gel-based detection platform.Type: GrantFiled: August 2, 2011Date of Patent: March 19, 2013Inventors: Darrel A. Mazzari, Martin A. Seitz
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Patent number: 8361808Abstract: Methods, materials, apparatus and systems are described for performing capillary flow assay. In one aspect, a system includes a sample collection unit to collect a sample liquid and a sample testing and storing unit to interface with the sample collection unit to test and store the collected sample liquid. The sample testing and storing unit includes a sample inlet shaped to receive the collected sample from the sample collection unit, and a sample well positioned below the sample inlet to retain at least a portion of the sample liquid. The sample testing and storing unit includes a sample housing unit to store a remainder of the sample liquid not retained in the sample well, and an analyte testing unit housing shaped to receive an analyte detecting unit to test a presence of a target analyte in the sample liquid.Type: GrantFiled: December 30, 2009Date of Patent: January 29, 2013Assignee: Oranoxis, Inc.Inventor: Dequn Wang
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Patent number: 8361810Abstract: Methods are disclosed for producing a bioweapon-sensitive fibrous-network product, wherein the subject products exhibit a color change in response to exposure to a biological agent (or portion thereof) as used in a biological weapon. Also disclosed are fibrous-network products that contain units of biopolymeric material that impart a color change to the products in response to exposure to a biological agent (or portion thereof) as used in a biological weapon.Type: GrantFiled: April 2, 2009Date of Patent: January 29, 2013Inventors: Dennis Farwell, Keith Baumann
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Patent number: 8329407Abstract: The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.Type: GrantFiled: February 9, 2011Date of Patent: December 11, 2012Assignee: The University of ChicagoInventors: Rustem F Ismagilov, Joshua David Tice, Cory John Gerdts, Bo Zheng
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Patent number: 8304193Abstract: The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.Type: GrantFiled: February 9, 2011Date of Patent: November 6, 2012Assignee: The University of ChicagoInventors: Rustem F Ismagilov, Joshua David Tice, Cory John Gerdts, Bo Zheng
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Patent number: 8278109Abstract: The present invention related to a pregnancy test device that can selectively detect hyperglycosylated human chorionic gonadotropin (hCG-H) in a liquid sample. The sample can be deposited on a proximal portion of the device for transport to a distal portion of the device. The device can include a release medium formed of a first material and including a detectable label thereon and a capture medium, including a capture site, in fluid communication with the release medium and formed of a second, different material. At least one of the release medium and the capture medium includes a binding member that exhibits a moderate to high affinity for hCG-H and is selectively or preferentially reactive with hCG-H.Type: GrantFiled: February 12, 2010Date of Patent: October 2, 2012Assignee: Church & Dwight Co., Inc.Inventors: Albert R. Nazareth, Timothy Snowden
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Patent number: 8252569Abstract: A microfluidic device for the concentration and lysis of cells or viruses and a method of concentrating and lysing cells or viruses using the microfluidic device include: magnetic beads, a reaction chamber in which the magnetic beads are accommodated and a laser source. The reaction chamber includes a plurality of electrodes which cross each other and are separated by a dielectric to generate an electric field and a vibrating part agitating the magnetic beads in the chamber. The laser source radiates a laser onto the magnetic beads in the reaction chamber.Type: GrantFiled: February 25, 2011Date of Patent: August 28, 2012Assignee: Samsung Electronics Co., Ltd.Inventors: Yoon-kyoung Cho, Jeong-gun Lee, Sung-young Jeong
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Patent number: 8236575Abstract: A first reactant, which is provided with a reaction site for specific binding with an analyte, and a fluorescent label site, and a second reactant, which is provided with a reaction site for specific binding with the analyte, and a fluorescence recognition site for recognizing fluorescence produced by the fluorescent label site of the first reactant, are respectively fixed onto a support such that the first reactant and the second reactant have a positional relationship adapted for the binding with the analyte.Type: GrantFiled: November 21, 2008Date of Patent: August 7, 2012Assignee: FUJIFILM CorporationInventors: Koichi Minami, Hirohiko Tsuzuki
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Patent number: 8232068Abstract: The present invention relates to the field of immunology and hyperproliferative diseases. More specifically, the present invention relates to a method of detecting and monitoring therapeutic antibody:antigen complex, soluble antigen and soluble therapeutic antibody, wherein a patient has undergone at least one course of immunotherapy. Yet further, levels of therapeutic antibody:antigen complexes, soluble antigens or soluble therapeutic antibodies may be measured and used to stage or monitor a hyperproliferative disease.Type: GrantFiled: January 12, 2012Date of Patent: July 31, 2012Assignee: Board of Regents, The University of Texas SystemInventors: Maher Albitar, Michael J. Keating, Taghi Manshouri
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Patent number: 8227263Abstract: A process for producing polymer particles having an antigen or an antibody introduced into the surface thereof, by carrying out miniemulsion polymerization using a monomer, a radical polymerization initiator, an emulsifier, and a hydrophobe in the presence of the antigen or antibody to thereby produce the polymer particles, is disclosed. According to the process, it is possible to provide a reagent for immunological analysis having an excellent detection sensitivity and capable of avoiding a nonspecific reaction which occurs in conventional methods.Type: GrantFiled: October 16, 2007Date of Patent: July 24, 2012Assignees: Mitsubishi Chemical Medience Corporation, National University Corporation Chiba UniversityInventors: Tatsuo Taniguchi, Akihiro Mizuno, Tokio Sawai, Hiroyuki Tsubota
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Patent number: 8216854Abstract: The present invention is directed to a system, device and method for measuring the concentration of an analyte in a fluid or matrix. A thermodynamically stabilized analyte binding ligand for use in the system, device and method is disclosed. The thermodynamically stabilized analyte binding ligand is resistant to degradation at physiological temperatures and its use within the device provides a minimally invasive sensor for monitoring the concentration of an analyte in a fluid or matrix as are present in the body of an animal.Type: GrantFiled: November 1, 2006Date of Patent: July 10, 2012Assignee: BioTex, Inc.Inventors: Ralph Ballerstadt, Roger McNichols, Ashok Gowda
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Patent number: 8183002Abstract: The present disclosure provides immunoassays and kits for detection or quantification of an analyte of interest in a test sample that potentially contains endogenously produced autoantibodies reactive with the analyte.Type: GrantFiled: December 3, 2009Date of Patent: May 22, 2012Assignee: Abbott LaboratoriesInventors: Maciej Adamczyk, Jeffrey R. Brashear, Phillip G. Mattingly
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Patent number: 8124359Abstract: A method for reducing non-specific binding in an assay is provided herein. The method includes (a) providing a reaction mixture, which includes or is suspected to include a first component and a second component capable of binding to each other in a specific binding reaction, and (b) adding non-physiological amounts of at least one additive to the reaction mixture before, during or after binding in a sufficient amount to reduce non-specific binding in the reaction mixture. The method further includes (c) monitoring or measuring the presence and/or concentration of at least one of the first and second components after step (b).Type: GrantFiled: March 16, 2007Date of Patent: February 28, 2012Assignee: Aokin AGInventors: Stuart Levison, Paul Nix, Derek Levison
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Patent number: 8114616Abstract: The invention provides an ELISA assay for the determination of serum mast cell ?-tryptase levels using rabbit anti-tryptase as the capture antibody and alkaline phosphatase conjugated G3 as the detecting antibody. Luminescent substrate CPSD was used to enhance the assay sensitivity. Also provided are methods for aiding in the diagnosis of irritable bowel syndrome by detecting the serum level of ?-tryptase, histamine and/or prostaglandin E2.Type: GrantFiled: August 24, 2010Date of Patent: February 14, 2012Assignee: Prometheus Laboratories Inc.Inventors: Hua Gong, Shui Long Wang, Sharat Singh
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Patent number: 8114661Abstract: The present invention is directed to the detection of target analytes using electronic techniques, particularly AC techniques.Type: GrantFiled: May 4, 2010Date of Patent: February 14, 2012Assignee: Osmetech Technology, Inc.Inventors: Stephen D. O'Connor, Jon Faiz Kayyem, Thomas J. Meade
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Patent number: 8114612Abstract: Assay systems and specialized antibodies for the detection and quantitation of troponin I and troponin T in body fluids as an indicator of myocardial infarction are disclosed. Troponin I and T exist in various conformations and the ratios of monomeric troponin I and T and the binary and ternary complexes, as well as which form of troponin present in the blood, may be related to the metabolic state of the heart. Disclosed are systems to determine the presence of a troponin form or a group of troponin forms in whole blood, serum or plasma samples. Disclosed is a method for improving the recovery of troponin I or T from a surface used in immunoassays. Also disclosed are antibodies which recognize unbound troponin forms, the forms of troponin in binary complexes, the ternary complex of troponin I, T and C, and the conformations of troponin I having intramolecularly oxidized and reduced cysteines.Type: GrantFiled: January 7, 2011Date of Patent: February 14, 2012Assignee: Alere San Diego, Inc.Inventors: Kenneth F. Buechler, Paul H. McPherson
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Patent number: 8114618Abstract: The present invention relates to the field of immunology and hyperproliferative diseases. More specifically, the present invention relates to a method of detecting and monitoring therapeutic antibody:antigen complex, soluble antigen and soluble therapeutic antibody, wherein a patient has undergone at least one course of immunotherapy. Yet further, levels of therapeutic antibody:antigen complexes, soluble antigens or soluble therapeutic antibodies may be measured and used to stage or monitor a hyperproliferative disease.Type: GrantFiled: April 5, 2011Date of Patent: February 14, 2012Assignee: Board of Regents of the University of Texas SystemInventors: Maher Albitar, Michael J. Keating, Taghi Manshouri
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Patent number: 8114408Abstract: We disclose synthetic peptide fragments comprising amino acid sequences of specific antigen sites of pathogenic Newcastle disease virus in birds, and more particularly synthetic peptide fragments that comprise polybasic amino acid sequences at the cleavage site of the F protein of pathogenic Newcastle disease virus and that induce humoral immune responses in hosts while reacting only with antibodies to pathogenic Newcastle disease virus and are useful to differentiate infected individuals from vaccinated individuals.Type: GrantFiled: November 26, 2008Date of Patent: February 14, 2012Assignee: Republic of Korea (Management: Ministry of Agriculture and Forestry, National Veterinary Research)Inventors: Kang Seuk Choi, Eun Kyoung Lee, Woo-Jin Jeon, Eun-Im Yoon, Jun-Hun Kwon
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Patent number: 8114615Abstract: The invention provides an improved method for identifying and interpreting tissue specimens and/or cells derived from tissue specimens. A panel of cell-based reagents provides a number of readouts of cellular states or biomarkers that together define a profile of a diversity of cellular states or biomarkers in a tissue specimen representing the “systems” nature of biology. This cellular profile is interpreted using informatics tools, to identify similarities between specimens, in vivo medical conditions, and suggest options for treating medical conditions.Type: GrantFiled: May 17, 2007Date of Patent: February 14, 2012Assignee: Cernostics, Inc.Inventors: Albert H. Gough, Kenneth A. Giuliano, D. Lansing Taylor
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Patent number: 8110365Abstract: Renal injury and inflammation is diagnosed by detecting an elevation in GSK3b level or activity. Inflammation of bodily tissues such as renal tissue is inhibited by administration of GSK3b inhibitory compositions.Type: GrantFiled: March 29, 2007Date of Patent: February 7, 2012Assignee: Rhode Island HospitalInventors: Lance Dworkin, Rujun Gong
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Patent number: 8110366Abstract: The invention describes specific sialylated structures present on human stem cells and cell populations derived thereof. The invention is especially directed to methods to control the status of stem cells by observing changes in sialylation of the cells; and control of potential contaminations of biological materials; and reagents and methods used in connection with the cells in order to avoid alterations of the cell glycosylation by contaminating materials. The invention is further directed to novel stem cells, the glycosylation of which has been specifically altered.Type: GrantFiled: July 7, 2006Date of Patent: February 7, 2012Assignees: Suomen Punainen Risti, Veripalvelu, Glykos Finland OyInventors: Jarmo Laine, Taina Jaatinen, Heidi Anderson, Johanna Nystedt, Sarl Tiitinen, Anita Laitinen, Ulla Impola, Tero Satomaa, Jari Natunen, Annamari Heiskanen, Maria Blomqvist, Anne Olonen
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Patent number: 8105788Abstract: Methods, systems, kits and assays for diagnosing, monitoring or screening for mucopolysaccharidosis (MPS) and methods and systems for assaying test compounds for therapeutic activity are described, whereby MPS marker protein levels are assayed as an indication of MPS.Type: GrantFiled: December 8, 2006Date of Patent: January 31, 2012Assignee: The University of British ColumbiaInventors: Lorne A. Clarke, Derrick Raymond Randall, Graham Sinclair