Abstract: A mushroom culture of Agaricus bisporus produced by hybridization of a first strain and a second strain of Agaricus bisporus, wherein at least one of said first and second strains of Agaricus is a hybrid mushroom culture of Agaricus bisporus designated strain J9277. Diverse additional strains can be developed from J9277 by various means including somatic and tissue culture selection, basidiospore selection, and hybridization to other strains of Agaricus bisporus, and the resulting derivative strains can be screened for desirable commercial characteristics. One resultant class of the mushroom Agaricus bisporus (J. Lange) Imbach is the hybrid strain J10165. It exhibits an attractive appearance that includes a smooth, bright white cap, and is biologically incompatible with strains of the Ur lineage group. A method for improving facility hygiene and reducing disease incidence at any commercial Agaricus bisporus mushroom production facility is also provided.

Abstract: A fungal bed cultivation method of a hon-shimeji mushroom, in which the sprouting step and/or fruit body growing step is carried out under an environmental condition of high CO2 concentration, is provided. Examples of the environmental condition of high CO2 concentration include a CO2 concentration of 2,500 ppm or more in the sprouting step and a CO2 concentration of 5,000 ppm or more in the fruit body growing step. Since the formation ratio of a budlet in the fungal bed cultivation of a hon-shimeji mushroom is improved by embodiments of the present invention, stable production of a hon-shimeji mushroom by its large scale commercial cultivation becomes possible.

Abstract: Ozone is applied to the interior of a mushroom house through use of an ozone generator which preferably communicates with an air conditioner so that the ozone flows from the ozone generator through the air conditioner and into the mushroom house. The ozone is applied during various stages of a mushroom growing process.

Abstract: The method of growing a fungal fruiting body requires exposing a mycelium of a desired organism type to environmental conditions sufficient to induce fruiting of fungal primordium in the organism type followed by enclosing the fungal primordium within a mold of a designated shape representing a near net shape volume of a desired final product. The fungal primordium is allowed to grow and fill the mold to form a mass of fungal tissue equivalent in shape to the designated shape of the mold after which the mass of fungal tissue is removed from the mold and dried.

Abstract: Hybrid Agaricus bisporus mushroom strains having one or more genetic characteristics of a wild mushroom strain deposited under ATCC accession No. PTA-6903 or a progeny thereof and having specified physical and genetic characteristics are disclosed along with methods of producing brown mushrooms for commercial use.

Abstract: Provided are a method of culturing Agaricus bisporus mycelium, the method comprising culturing Agaricus bisporus mycelium or spores thereof in a liquid medium comprising sugar cane extract, and a medium for culturing the Agaricus bisporus mycelium.

Abstract: The use of dewatered small particle tailings material derived from the industrial washing of coal, metal ores, rocks and extracted minerals as a component of casing material for the cultivation of mushrooms (Agaricus bisporus and other Agaricus spp.) is described.

Abstract: The present invention relates to an artificial cultivating technique of fruiting body of Antrodia camphorate. The method concludes a process for cultivating Antrodia camphorata on camphor tree, which comprises liquid culture of Antrodia camphorata, treatment of camphor tree, and useage of essential oil, to cultivate Antrodia camphorata on the segmental woods of camphor tree. The efficiency of successful cultivation is confirmed by micrescopy and HPLC analysis, and compared with that of natural growth.

Abstract: The present invention discloses an apparatus which is designed to hold a plurality of cultivation beds for mushrooms or other agricultural products, each rotating on two different axes. The three disclosed embodiments operate according to different configurations but all provide similar functionality for the apparatus, enabling the frames holding the cultivation beds to rotate in two different circular motions independently or in congruence. The overall design of the preferred embodiments of the apparatus resemble that of an elaborate Ferris wheel and includes a central wheel assembly, and a number of secondary wheel assemblies each holding a plurality of cultivation beds which are positioned on the perimeter of the central wheel assembly. The first rotating motion is around the axis of the central wheel and the second is of each secondary rotating mechanism around its axis.

Abstract: According to the present invention, there is provided a fungal bed cultivation method of a hon-shimeji mushroom, wherein induction of primordia of a fruit body is conducted by illumination under incubation conditions, and an ongoing culture for fungal bed cultivation of a hon-shimeji mushroom, wherein primordia of a fruit body have been formed thereon during incubation. The culture medium is maintained in a clean state and it is advantageous because the formation of primordia of a fruit body can be carried out in a clean environment and the culture medium wherein primordia of a fruit body have been formed can be transferred to distant mushroom cultivation facilities under clean conditions as such.

Abstract: Problem: To provide a method for fungal bed cultivation of a mushroom of a large size having an excellent shape and crunchy texture, a mushroom cutting useful for the fungal bed cultivation method, a culture medium for fungal bed cultivation into which the cutting is transplanted, and a culture medium suitable for fungal bed cultivation. Solution: A method for cultivating a mushroom in fungal bed comprising a step of transplanting an isolated cutting of the mushroom into a culture medium for fungal bed cultivation, an isolated cutting of a mushroom to be used in the method, and a culture medium for fungal bed cultivation of a mushroom into which the cutting is transplanted.

Abstract: Ozone is applied to the interior of a mushroom house through use of an ozone generator which preferably communicates with an air conditioner so that the ozone flows from the ozone generator through the air conditioner and into the mushroom house. The ozone is applied during various stages of a mushroom growing process.

Abstract: A process and device used to produce CO2 in an indoor environment is described. The device contains a container that contains a mixture of substrate and fungi. The container has at least one opening to permit the CO2 to enter the plant growth environment and a pump connected to the container to pump out the CO2 through said opening into the plant growth environment. The invention further relates to a kit that contains a container, substrate, fungi and a pump.

Abstract: The interior of a mushroom house is sterilized by applying ozone into the interior of the mushroom house around compost in receptacles in the mushroom house. The ozone is applied prior to the spawning step in the growing of the mushrooms.

Abstract: A composition and method for the formulation of ingredients for a medium for growing fungi and the like. The invention provides for a formulation of a growing medium including oat hulls for the cultivating of mushroom mycelium.

Abstract: Methods of increasing the amount of one or more functional compounds in medicinal mushroom biomass and mushroom mycelia by growing the mushrooms on a substrate that is high in the functional compound or compounds of interest is provided. More specifically, a method of increasing the amount of anthocyanins in mushrooms is provided. Mushrooms are grown on a substrate rich in anthocyanins, such as purple corn or other suitable red, purple or black-pigmented substrate, and incorporate the anthocyanins during growth. Mushrooms grown by this method, as well as methods of improving the growth rate and potency of mushrooms, are also provided.

Abstract: The present invention relates to the establishment of a cultivation condition that is suitable for the large-scale production of pharmacologically active filtrates from a culture of A. camphorata, in particular, by optimizing the agitation rate and/or pH value during the cultivation. The present invention also relates to a process for obtaining pharmacologically active compositions from a culture of A. camphorata through a series of fractionation. This invention is further directed to the uses of the above compositions in the preparation of pharmaceutical compositions.

Abstract: An incubation method for obtaining solid culture of Zang Zhi (Antrodia camphorata) including the steps of: incubating a stock strain of Zang Zhi in a PP bag for growth of mycelia, followed by an incubation in the air for fruiting the Zang Zhi body.

Abstract: The present invention relates to the establishment of a cultivation condition that is suitable for the large-scale production of pharmacologically active filtrates from a culture of A. camphorata, in particular, by optimizing the agitation rate and/or pH value during the cultivation. The present invention also relates to a process for obtaining pharmacologically active compositions from a culture of A. camphorata through a series of fractionation. This invention is further directed to the uses of the above compositions in the preparation of pharmaceutical compositions.

Abstract: A reusable filter port assembly is installable on the wall of a sterilizable cultivation bag, such as is used in the cultivation of mushrooms. A grommet and locking collar are removably installed in the wall of the cultivation bag or pouch to provide gas flow communication between the interior of the bag and the ambient environment after the bag is sealed. A removable filter cap holds a filter member over the opening of the filter port assembly to prevent undesired particles from passing through the port of the assembly. In use, the grommet is placed against the interior surface of a bag or pouch, such as a plastic cultivation bag. The collar is screwed down over the grommet from the exterior surface of the bag to form a grommet-collar combination fixed in place to the wall of the bag. Once installed, a patch of bag material of the bag wall is still covering the grommet opening of the neck tube.

Abstract: The present invention describes a new substrate comprising olive oil waste as the nutrient for growing edible and medicinal shiitake mushrooms (Lentinus edodes) in aseptic plastic bags. The invention further provides the strain Ile-1, a new and distinct variety of the species Lentinus edodes, that grows very efficiently in the olive oil waste-containing substrate.

Abstract: The present invention relates to the establishment of a cultivation condition that is suitable for the large-scale production of pharmacologically active filtrates from a culture of A. camphorata, in particular, by optimizing the agitation rate and/or pH value during the cultivation. The present invention also relates to a process for obtaining pharmacologically active compositions from a culture of A. camphorata through a series of fractionation. This invention is further directed to the uses of the above compositions in the preparation of pharmaceutical compositions.

Abstract: A process for cultivating Morchella ascocarps using mycelium and a tree seedling, the tree seedling having a root system and a shoot system. The process includes the steps of inoculating the root system with the mycelium to produce an inoculated tree seedling; stimulating the mycelium to form sclerotia by distressing the inoculated tree seedling; and inducing the sclerotia to produce ascocarps by providing conditions conducive to the formation of ascocarps. Also, the growth of trees may be accelerated by inoculating the root system with the mycelium. Further more, a process for cultivating Morchella sclerotia using mycelium and a tree seedling, the tree seedling having a root system and a shoot system includes the steps of inoculating the root system with the mycelium to produce an inoculated tree seedling; and stimulating the mycelium to form sclerotia by distressing the inoculated tree seedling.

Abstract: A process for cultivating Morchella ascocarps using mycelium and a tree seedling, the tree seedling having a root system and a shoot system. The process includes the steps of inoculating the root system with the mycelium to produce an inoculated tree seedling; stimulating the mycelium to form sclerotia by severing the shoot system from the root system; and inducing the sclerotia to produce ascocarps by providing conditions conducive to the formation of ascocarps. Also, the growth of trees may be accelerated by inoculating the root system with the mycelium.

Abstract: Methods are described for producing reduced, methylated, biologically active forms of folate, including 5-methyltetrahydrofolate (i.e. 5-methyltetrahydropteroylglutamic acid), 5-formyltetrahydrofolate, 10-formyltetrahydrofolate and tetrahydrofolate. Edible mushroom-producing fungi are cultivated to enhance the uptake of pteroylmonoglutamate (synthetic folate) into edible portions of the plants. The mushroom-producing fungi reduce and methylate the pteroylmonoglutamates into activated folates. The cultivated mycelia or mushrooms of the mushroom-producing fungi are harvested, and may be consumed directly or processed into oral dosage formats (capsules, tablets, softgels, powders, gel packets, liquids, nutritional bars, beverages) for use as functional foods or nutritional supplements.

Abstract: A method of forming an artificial Shiro of Matsutake comprises culturing Matsutake hyphae in a culture substrate containing a substance capable of controlling the cell membrane permeability of the hyphae as an active principle, and a method of forming an artificial Shiro of Matsutake comprises culturing Matsutake hyphae in a culture substrate containing a substance capable of enhancing the hydrophilic property of the hyphae as an active principle.

Abstract: A novel hybrid mushroom culture of Agaricus bisporus, and its inbred and outcrossed descendants, is produced by crossing a culture of a wild strain of Agaricus bisporus designated RWK 1913 with a second culture of a different, preferably cultivated, strain of Agaricus bisporus. Where the second culture is the known cultivated strain designated S130-b, a novel hybrid mushroom culture designated B7970 is formed.

Abstract: Provided is a method for the in vitro aseptic mass production and sporulation of arbuscular mycorrhizal fungi (AMF). The method comprises providing symbiotic root organs with AMF propagules and spores after the root organs are inoculated with AMF inoculums in advance to form AMF propagules in the root organs and cultivating them in an aseptic container, and then providing the whole symbiotic root organs and AMF propagules with liquid medium for a temporary contact between the liquid medium and the root organ with AMF propagules in order to cultivate AMF, then removing the liquid medium from the symbiotic root organs and arbuscular mycorrhizal fungal propagules and finally repeating former two steps periodically to facilitate the mass production and sporulation of the arbuscular mycorrhizal fungi.

Abstract: A self-automated mushroom growing apparatus and method for providing a continuous water and air supply for growing mushrooms on a small-scale. The apparatus is an enclosed, terrarium-like Grow Space including a porous Wicking Medium substrate, and an Air Diffuser rod situated within or below the porous Wicking Medium. An Air Diffuser is situated within the apparatus and is connected to an air supply to provide fresh air to the Grow Space. The porous Wicking Medium is saturated with water. The saturated Wicking Medium provides a continuous supply of water to a Cake Culture in contact with the Wicking Medium. The Air Supply mixes stale air that settles near the surface of the porous Wicking Medium.

Abstract: Preventive and therapeutic agents against the HIV virus and pathogenic bacteria, comprising the active ingredients obtained from hyphae of Kabanoanatake [scientific name: Fuscoia obliqua (Fr.) Aoshima].

Abstract: The present invention relates to the establishment of a cultivation condition that is suitable for the large-scale production of pharmacologically active filtrates from a culture of A. camphorata, in particular, by optimizing the agitation rate and/or pH value during the cultivation. The present invention also relates to a process for obtaining pharmacologically active compositions from a culture of A. camphorata through a series of fractionation. This invention is further directed to the uses of the above compositions in the preparation of pharmaceutical compositions.

Abstract: The present invention relates to the establishment of a cultivation condition that is suitable for the large-scale production of pharmacologically active filtrates from a culture of A. camphorata, in particular, by optimizing the agitation rate and/or pH value during the cultivation. The present invention also relates to a process for obtaining pharmacologically active compositions from a culture of A. camphorata through a series of fractionation. This invention is further directed to the uses of the above compositions in the preparation of pharmaceutical compositions.

Abstract: This invention provides a tray liner that is insertable into a mushroom-growing tray. This invention provides a tray liner that provides protection to the mushroom-growing tray by eliminating direct contact of the growing medium or compost with the trays' interior wood The tray liner has a rectangular base member with a periphery of upstanding sidewalls joined to the base portion. There is a notch section located at each corner of the rectangular base member that accommodates an upstanding corner frame member located at each corner of the wooden mushroom growing tray. The notch section has a contiguous wall that is joined with the periphery of upstanding sidewalls and the base member. An alternative embodiment is to provide a replaceable mushroom growing tray liner that comprises a plurality of sections.

Abstract: A process for cultivating Morchella ascocarps using mycelium and a tree seedling, the tree seedling having a root system and a shoot system. The process includes the steps of inoculating the root system with the mycelium to produce an inoculated tree seedling; stimulating the mycelium to form sclerotia by distressing the inoculated tree seedling; and inducing the sclerotia to produce ascocarps by providing conditions conducive to the formation of ascocarps. Also, the growth of trees may be accelerated by inoculating the root system with the mycelium. Further more, a process for cultivating Morchella sclerotia using mycelium and a tree seedling, the tree seedling having a root system and a shoot system includes the steps of inoculating the root system with the mycelium to produce an inoculated tree seedling; and stimulating the mycelium to form sclerotia by distressing the inoculated tree seedling.

Abstract: Disclosed herein is a biological method for aerobically treating whey using mushroom mycelia wherein the whey can be biologically treated at a disposal rate comparable to conventional methods. According to the method, since environmentally unfriendly sludge is not discharged, post-treatment operations in connection with the disposal of sludge can be simplified. Therefore, the method can lower environmental costs and further enables the economical cultivation of mushroom mycelia. Furthermore, the method has an advantage in terms of resource recycling.

Abstract: A process for cultivating Morchella ascocarps using mycelium and a tree seedling, the tree seedling having a root system and a shoot system. The process includes the steps of inoculating the root system with the mycelium to produce an inoculated tree seedling; stimulating the mycelium to form sclerotia by severing the shoot system from the root system; and inducing the sclerotia to produce ascocarps by providing conditions conducive to the formation of ascocarps. Also, the growth of trees may be accelerated by inoculating the root system with the mycelium.

Abstract: A substrate for mushroom cultivation comprises a polyene fungicide, in particular, natamycin. Mushrooms cultivated in such substrates can be harvested earlier than mushrooms cultivated in substrates which do not include polyene fungicides. Alternatively, mushrooms grown in a substrate comprising a polyene fungicide achieve a greater size than mushrooms grown for the same amount of time in a substrate which does not include a polyene fungicide.

Abstract: This invention provides a tray liner that is insertable into a mushroom-growing tray. This invention provides a tray liner that provides protection to the mushroom-growing tray by eliminating direct contact of the growing medium or compost with the trays' interior wood The tray liner has a rectangular base member with a periphery of upstanding sidewalls joined to the base portion. There is a notch section located at each corner of the rectangular base member that accommodates an upstanding corner frame member located at each corner of the wooden mushroom growing tray. The notch section has a contiguous wall that is joined with the periphery of upstanding sidewalls and the base member. An alternative embodiment is to provide a replaceable mushroom growing tray liner that comprises a plurality of sections.

Abstract: This invention provides a tray liner that is insertable into a mushroom-growing tray. This invention provides a tray liner that provides protection to the mushroom-growing tray by eliminating direct contact of the growing medium or compost with the trays' interior wood The tray liner has a rectangular base member with a periphery of upstanding sidewalls joined to the base portion. There is a notch section located at each corner of the rectangular base member that accommodates an upstanding corner frame member located at each corner of the wooden mushroom growing tray. The notch section has a contiguous wall that is joined with the periphery of upstanding sidewalls and the base member. An alternative embodiment is to provide a replaceable mushroom growing tray liner that comprises a plurality of sections.

Abstract: A growth medium for plants or mushrooms, the medium comprising sugar care mill mud and non sphagnum-peat material selected from the group consisting of a non-sphagnum-peat and coconut fibre.

Abstract: The present invention provides an improved nutrient supplement that increases the yield of mushrooms, especially Agaricus bisporus. The nutrient supplement consists of polysaccharides (especially cellulose) or polysaccharide-containing materials. The polysaccharides or polysaccharide-containing materials may be mixed with protein-containing and/or vegetable oil-containing materials and/or may be treated to increase the availability of the carbohydrate to the mushroom fungus.

Abstract: An in vitro system using Agrobacterium-transformed dicotyledon roots (such as carrot roots) and vesicular-arbuscular mycorrhizal fungi (such as Glomus intradices) was developed to produce viable and aseptic spores. The entire life cycle from the growth of initial germ tubes to the formation of external mycelium network and spore production was observed nondestructively using light microscopy. The system may be subcultured continuously for the production of viable and clean spores.

Abstract: Disclosed is an efficient method for culturing an edible basidiomycetous fungus such as Mushroom Agaricus Blazei Murill in a liquid culture medium to give fungus aggregates of several centimeter size. Characteristically, the liquid culture medium is formulated with sucrose as a carbon source in the form of crude cane sugar in combination with a water-insoluble growth-supporting material in the form of a fine powder to serve as the core of the fungus aggregates as selected from crushed sugarcane, sugarcane bagasse, pine trees and wheat bran. Further characteristically, the culturing procedure is carried out under an oxygen-enriched condition by blowing oxygen-enriched air of at least 30% by volume oxygen into the culture medium under pressurization at 0.12 to 0.5 MPa (absolute) in a specified blowing rate.

Abstract: “Novel hybrid strains of Agaricus bisporus, the germination of the spores of which provides mostly homocaryons, their production and their use”. Hybrid strain of Agaricus having the following traits: it is interfertile with Agaricus bisporus, its fructifications have basidia of which less than 15% are bisporous, and most of the spores on said fructifications are homocaryotic, and whose traits can be genetically transmitted; method of obtaining such a strain, and its use for obtaining modified strains of Agaricus bisporus.

Abstract: Mushrooms harvested from a growing bed are deposited on a carrier having a plurality of individual recesses each for receiving an individual mushroom by supporting the under-side of the cap with the stalk projecting downwardly. The carrier, when fully loaded with mushrooms, is moved into a compartment of a multi-compartmented storage structure configured to allow the flow of air therethrough. The structure is then moved into a cold room so that all of the carried mushrooms are cooled down to the optimum storage temperature. Only when the mushrooms are to be dispatched for sale are the carriers removed from the storage structure and the stalks of the mushrooms trimmed to the required length whereafter the mushrooms are suitably packed for dispatch and sale.

Abstract: Disclosed is an efficient method for culturing an edible basidiomycetous fungus such as Mushroom Agaricus Blazei Murill in a liquid culture medium to give fungus aggregates of several centimeter size. Characteristically, the liquid culture medium is formulated with sucrose as a carbon source in the form of crude cane sugar in combination with a water-insoluble growth-supporting material in the form of a fine powder to serve as the core of the fungus aggregates as selected from crushed sugarcane, sugarcane bagasse, pine trees and wheat bran. Further characteristically, the culturing procedure is carried out under an oxygen-enriched condition by blowing oxygen-enriched air of at least 30% by volume oxygen into the culture medium under pressurization at 0.12 to 0.5 MPa (absolute) in a specified blowing rate.

Abstract: A pharmacological composition including a component having pressure reductive activity obtained from Mycoleptodonoides aitchisonii fruit body which is safe and cheap, harvested constantly year-round by artificial cultivation, with equal quality, and useful for prevention and/or amelioration of symptom of hypertension caused by angiotensin I converting enzyme or the like. The invention also includes foods or beverages containing the pharmacological compositions, and methods of preventing or treating hypertension by administration of the composition.

Abstract: The present invention describes new and distinct strains of higher Basidiomycetes mushrooms grown in submerged cultures. Specifically, the new strain of species of the genus Tremella offer superior yields of one-cell biomass and exocellular heteropolysaccharide glucuronoxylomannan, niacin and essential amino acids.

Abstract: The present invention relates to a mushroom bed cultivation method for cultivating a fruit body of Agaricus and a grain-containing medium used for such a method. The method includes the step of culturing Agaricus in a medium including a grain. The medium further includes a nutrient, a medium matrix, and the like, as necessary.

Abstract: The present invention describes new and distinct horticultural varieties of higher Basidiomycetes mushrooms grown in submerged culture. Specifically, the new varieties of species of the genus Pleurotus offer superior yields of mushroom cell biomass and greater concentrations of the protein rich in essential amino acids, fatty acids, vitamins and minerals.