Abstract: A diagnostic device for and method of detecting the presence of head and neck squamous cell carcinoma (HNSCC) in a patient including a detector device for detecting a presence of at least one marker indicative of HNSCC, the detector device including a panel of markers for HNSCC. A diagnostic device for and method of staging HNSCC in a patient including a detector device for detecting a presence of at least one marker indicative of stages of HNSCC, the detector device including a panel of markers for HNSCC. Markers for head and neck squamous cell carcinoma selected from the markers listed in Table 5. Methods of personalized immunotherapy, making a personalized anti-cancer vaccine, and predicting a clinical outcome in a HNSCC patient. A method of making a panel of HNSCC markers.

Abstract: The present invention includes composition and methods for making and using a combinatorial library having two or more beads, wherein attached to each bead is a unique nucleic acid aptamer that have disposed thereon a unique sequence. The library aptamers may be attached covalently to the one or more beads, which may be polystyrene beads. The aptamers may include phosphorothioate, phosphorodithioate and/or methylphosphonate linkages and may be single or double stranded DNA, RNA or even PNAs.

Abstract: A method for manufacturing a biochip is provided. First, a first self-assembled monolayer is coated on a substrate. Next, a plurality of first biomedical molecular dots are formed on the first self-assembled monolayer by micro-titration technique. After the bonding reaction between the first biomedical molecular point and the first self-assembled monolayer, a second self-assembled monolayer is deposited on the surface of the first self-assembled monolayer by evaporation. The second self-assembled monolayer attached on the plurality of first biomedical molecular dots and the first self-assembled monolayer not bonded to the substrate are removed, so that the first biomedical molecular dots immobilized on the first self-assembled monolayer are exposed. Finally, a second biomedical molecular layer is immobilized on the exposed portions of the first biomedical molecular dots.

Abstract: This invention relates to a kit and a system for performing an in-solution microarray assay, and a method of carrying out this assay. The kit includes a plate having a number of wells in which a plurality of magnetic beads are placed. These magnetic beads are coated with different probes, wherein beads placed in each individual well are coated with identical probes.

Abstract: A technique is disclosed for performing sequencing of polymers such as DNA and RNA. A sample containing multiple sites to be sequenced is cyclically subjected to attachment of nucleotides, and imaging. A digital mask corresponding to sites of interest in the sample array is generated and image data for the sites is processed differently from image data not corresponding to sites of interest. The latter may be discarded during the sequencing operation. The use of the mask improves computational efficiency and reduces memory allocated for the image data during sequencing.

Abstract: The present invention provides novel methods and reagents for detecting the binding of protein targets to nucleic acid ligands. Using Universal Protein Stains (UPS), proteins bound by nucleic acid ligands may be labeled with a detectable moiety. The methods and reagents are particularly useful for the detection of protein targets bound to multiplexed arrays of nucleic acid ligands. The present invention also provides novel methods for the multiplexed evaluation of photocrosslinking nucleic acid ligands. The methods allow one simultaneously to: (1) evaluate the performance (dynamic range) of a plurality of photocrosslinking nucleic acid ligands; and (2) assess the specificity of each photocrosslinking nucleic acid ligand for its cognate target protein. Photocrosslinking nucleic acid ligands with the most desirable properties can then be selected for use in diagnostic and prognostic medical assays.

Abstract: Biopolymeric array scanners that are capable of automatically selecting a dye specific scale factor to employ for a plurality of different dyes, as wells as methods for making and using the same, are provided. In many embodiments, the actual dye specific scale factor automatically selected by the scanner is one that is equal to a preset “master” scale factor, so that the scanner reads any supported dye using the same constant scale factor. The dye specific scale factor selection is typically made by reference to a collection of nominal scale factors for each member of the plurality of dyes. In using the subject scanners, a user simply inputs the one or more dyes being used in a given array assay, and the scanner automatically reads the array using an automatically chosen dye specific scale factor for the selected dyes. Also provided are methods of obtaining collections of nominal scale factors and computer readable mediums comprising the same.

Abstract: A biosensor includes an analysis unit and a reader device for detecting an analyte. In at least one embodiment, the analysis unit includes a quartz oscillator on whose surface capture molecules, which bind specifically to the analyte, are immobilized; as well as a transponder to transmit information, regarding whether and/or how many analyte molecules have bound to the capture molecules, to the reader device.

Abstract: A method and means of identifying nucleic acid oligomers is disclosed. A sample is split into parts and the parts are flowed through chromatography columns containing nucleic acid oligomer probes bound to a binding medium. Analyte oligomers are transiently hybridized to complementary probe oligomers during chromatography. Detection and analysis of oligomer peaks is used to identify the oligomers contained in the sample.

Abstract: A nucleic acid cassette includes a main body in which first and second channels are formed. A sample containing a target nucleic acid is input in a sample chamber of the second channel via an injection port. In the cassette, a detecting part includes a detection chamber communicated to the first and second channels and a nucleic detection unit. The first channel has a first reagent reservoir storing a first reagent and a second reagent reservoir storing a second reagent. The first and second reagents reservoirs are separated by a gaseous body in the channel. The sample and the first and second reagents are supplied to the detecting part due to an action of a pump part communicated to the first and second channels.

Abstract: The present invention provides microdevices for electrochemical detection of target agents in a fluid sample. The microdevices each comprise an elongated microstructure having an inlet for introduction of a fluid sample potentially containing a target agent, and an outlet to allow the sample to flow through the region of the microdevice that allows detection of the target agent. This detection is mediated through hybridization of nucleic acids corresponding to a target agent with device-associated nucleic acids and subsequent binding of an electrochemical detection agent to allow the transfer of an electron to or from the electrode. In specific embodiment, the device comprises: a hollow elongated microstructure with a conductive surface on the internal surface; b) insulating polymer that is uniformly distributed on the conductive surface; c) adapter molecule associated with the insulating polymer, and d) a plurality of associated nucleic acids conjugated to the polymer surface in a specific orientation.

Abstract: The present application relates to a system and apparatus for protein sequencing that incorporates a single reaction vessel with multiple selectable receiver ports system that allows for high throughput and flexibility in the use of a detection system to provide a robust sequencing system.