Method Of Screening A Library Patents (Class 506/7)
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Patent number: 11977077Abstract: The present invention relates to pancreatic cancers and the use of biomarkers in biological samples for the diagnosis of such conditions, in particular pancreatic ductal adenocarcinoma. The biomarker panel comprises LYVE1, REG1 and TFF1. Methods of treatment are also provided, as well as kits useful in the diagnosis and treatment of pancreatic ductal adenocarcinoma. The present invention is particularly useful in detecting early-stage PDAC.Type: GrantFiled: August 26, 2020Date of Patent: May 7, 2024Assignee: Queen Mary University of LondonInventors: Tatjana Crnogorac-Jurcevic, Tomasz Radon
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Patent number: 11967078Abstract: The disclosure herein relates to systems, methods, and devices for medical image analysis, diagnosis, risk stratification, decision making and/or disease tracking. In some embodiments, the systems, devices, and methods described herein are configured to analyze non-invasive medical images of a subject to automatically and/or dynamically identify one or more features, such as plaque and vessels, and/or derive one or more quantified plaque parameters, such as radiodensity, radiodensity composition, volume, radiodensity heterogeneity, geometry, location, perform computational fluid dynamics analysis, facilitate assessment of risk of heart disease and coronary artery disease, enhance drug development, determine a CAD risk factor goal, provide atherosclerosis and vascular morphology characterization, and determine indication of myocardial risk, and/or the like.Type: GrantFiled: December 30, 2022Date of Patent: April 23, 2024Assignee: Cleerly, Inc.Inventors: James K. Min, James P. Earls, Hugo Miguel Rodrigues Marques, Shant Malkasian, Ben Hootnick
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Patent number: 11903735Abstract: An apparatus and method for examining an organism surface includes a pliable member including a contact layer, a plurality of channels, and a reaction layer, wherein the plurality of channels are disposed between the contact layer and the reaction layer; each of the channels having a size in the range of micrometers and formed of biocompatibility materials, and the contact layer including a plurality of spherical projections having a size in the range of micrometers and formed of biocompatible materials, and the reaction layer including examining molecules and a reaction unit.Type: GrantFiled: April 30, 2021Date of Patent: February 20, 2024Assignees: CHANG GUNG UNIVERSITY, CHANG GUNG MEMORIAL HOSPITAL, LINKOUInventors: Hsin-Yao Wang, Ting-Wei Lin, Jang-Jih Lu
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Patent number: 11887720Abstract: The present disclosure is generally directed to an apparatus for using a feedback loop to optimize meals, may include at least a processor; and a memory communicatively connected to the processor, the memory containing instructions configuring the at least a processor to retrieve nutrition data from a database. The processor may be configured to generate an optimization score, wherein generating the optimization score may include training an optimization machine-learning model, wherein the optimization machine-learning model is trained with optimization training data, inputting a nutrient quantity to the optimization machine-learning model to output a target nutrient score, and generating an optimization score as a function of the nutrition data and the target nutrient score.Type: GrantFiled: January 23, 2023Date of Patent: January 30, 2024Inventor: Kenneth Neumann
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Patent number: 11860108Abstract: An optofluidic diagnostic system and methods for rapid analyte detections. The system comprises an optofluidic sensor array, a test plate and an optical detection cartridge. The sensor array supports one or more distinct sensor units, each having a reactor section designed to temporarily enter a series of different kinds of wells in the test plate. One kind of well is a sample reservoir that holds reagent solution to be transferred into the reactor section. Another kind of well is a drainage chamber that removes reagent solution from the reactor section. A third kind of well is a colorant reservoir that holds a colorant reagent transferable into a reactor section. Finally, the sensor unit is transferred to the optical detection cartridge where it is placed into an isolation booth during the optical detection process so that its flat observation face is stationed in a viewing window opposite an optical detector lens.Type: GrantFiled: October 6, 2022Date of Patent: January 2, 2024Assignee: Optofluidic Bioassay, LLCInventors: Maung Kyaw Khaing Oo, Xudong Fan
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Patent number: 11851710Abstract: This document provides methods and materials for identifying metastatic malignant skin lesions (e.g., malignant pigmented skin lesions). For example, methods and materials for using quantitative PCR results and correction protocols to reduce the impact of basal keratinocyte contamination on the analysis of test sample results to identify metastatic malignant skin lesions are provided. This document also provides methods and materials for treating skin cancer. For example, methods and materials for identifying a mammal (e.g., a human) having a pre-metastatic skin lesion (e.g., pre-metastatic melanoma) and treating that mammal with pentamidine (4,4?-[pentane-1,5-diylbis(oxy)]dibenzenecarboximidamide) are provided.Type: GrantFiled: September 20, 2019Date of Patent: December 26, 2023Assignee: Mayo Foundation for Medical Education and ResearchInventors: Alexander Meves, Ekaterina M. Nikolova
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Patent number: 11795498Abstract: Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3? end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3? of the positional domain.Type: GrantFiled: February 16, 2023Date of Patent: October 24, 2023Assignee: 10x Genomics Sweden ABInventors: Jonas Frisen, Patrik Stahl, Joakim Lundeberg
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Patent number: 11788122Abstract: Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3? end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3? of the positional domain.Type: GrantFiled: October 17, 2022Date of Patent: October 17, 2023Assignee: 10x Genomics Sweden ABInventors: Jonas Frisen, Patrik Stahl, Joakim Lundeberg
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Patent number: 11725231Abstract: The present disclosure provides methods and systems for nucleic acid preparation and/or analysis. Nucleic acids may be derived from one or more cells. Nucleic acid preparation may comprise generating nucleic acid molecules of varying lengths. Nucleic acid analysis may comprise identifying nucleic acid sequence information with nucleosome position information.Type: GrantFiled: April 21, 2020Date of Patent: August 15, 2023Assignee: 10x Genomics, Inc.Inventor: Geoffrey McDermott
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Patent number: 11702700Abstract: The disclosure provides methods of identifying a human subject as a candidate for treating or inhibiting a liver disease by inhibiting HSD17B13. The disclosure also provides methods of treating a subject who is PNPLA3 Ile148Met+ by administering an inhibitor of HSD17B13. The disclosure also provides method of detecting a PNPLA3 Ile148Met variant and functional HSD17B13 in a subject. The disclosure also provides method of identifying a subject having a protective effect against liver disease. The disclosure also provides inhibitors of HSD17B13 for use in the treatment of a liver disease.Type: GrantFiled: February 18, 2021Date of Patent: July 18, 2023Assignee: Regeneron Pharmaceuticals, Inc.Inventors: Yurong Xin, Jesper Gromada, Xiping Cheng, Frederick Dewey, Tanya Teslovich Dostal, Claudia Schurmann, Aris Baras, Noura Abul-Husn
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Patent number: 11629379Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.Type: GrantFiled: April 13, 2022Date of Patent: April 18, 2023Assignee: President and Fellows of Harvard CollegeInventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
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Patent number: 11608527Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.Type: GrantFiled: April 13, 2022Date of Patent: March 21, 2023Assignee: President and Fellows of Harvard CollegeInventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
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Patent number: 11561218Abstract: The invention relates to a method for detecting a dengue infection in a patient blood sample, comprising the steps: a) Performing an analysis of prespecified parameters of blood platelets and prespecified types of blood cells in the sample and determining parameter values for the prespecified parameters of the platelets and the prespecified types of cells; b) Obtaining sample parameters from the values determined in step a); and c) Evaluating the sample parameters in relation to a prespecified criterion, wherein, if the criterion is fulfilled, a dengue infection is present.Type: GrantFiled: March 8, 2019Date of Patent: January 24, 2023Assignee: Siemens Healthcare Diagnostics he.Inventors: Kieran O'Donoghue, Rory Sobolewski
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Patent number: 11554370Abstract: Described herein are systems and methods for analyzing biological samples. Including a method for processing an analyte, comprising providing a fluidic device comprising the analyte and one or more polymer precursors; selecting a discrete area within said fluidic device; providing an energy source in optical communication with fluidic device; and selectively supplying a unit of energy generated from the energy source to the fluidic device to generate a polymer matrix within the fluidic device, wherein the polymer matrix is within the discrete area or adjacent to the discrete area.Type: GrantFiled: April 7, 2022Date of Patent: January 17, 2023Assignee: Cellanome, Inc.Inventors: Tarun Kumar Khurana, Ali Agah, Yir-Shyuan Wu, Filiz Gorpe Yasar
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Patent number: 11534758Abstract: A micro-total analysis system and a method thereof are provided. The micro-total analysis system includes: a microfluidic device, configured to accommodate a liquid to be detected; an optical unit, configured to form a first light irradiated to the microfluidic device; and a detection unit, configured to detect the liquid to be detected and output a detection signal to obtain detection information.Type: GrantFiled: August 16, 2018Date of Patent: December 27, 2022Assignee: BOE TECHNOLOGY GROUP CO., LTD.Inventors: Xue Dong, Yanling Han, Haisheng Wang, Chuncheng Che, Xiaoliang Ding, Yingming Liu, Yuzhen Guo, Wanxian Xu, Peizhi Cai, Haochen Cui, Fengchun Pang
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Patent number: 11534392Abstract: A cosmetic composition including exosomes derived from Galactomyces as an active ingredient is provided for skin regeneration, skin elasticity improvement or skin wrinkle reduction. The cosmetic composition has excellent effects on skin regeneration, skin elasticity improvement and/or skin wrinkle reduction.Type: GrantFiled: February 9, 2021Date of Patent: December 27, 2022Assignee: ExoCoBio Inc.Inventors: Yong Weon Yi, Byong Seung Cho
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Patent number: 11536730Abstract: The invention relates to methods for determining whether a subject is afflicted with a motor neuron disease, the method comprising conducting an analysis of cerebrospinal fluid and/or plasma, measuring the level of one or more sterol/oxysterol analytes, and comparing these to reference values. Further, the invention relates to methods of identifying agents suitable for the treatment of MND, and monitoring the progress of the disease.Type: GrantFiled: July 4, 2017Date of Patent: December 27, 2022Assignee: Swansea UniversityInventors: William Griffiths, Peter Crick, Yuqin Wang, Jens Kuhle, Andreas Sailer, Juan Zhang, Martin Turner
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Patent number: 11511277Abstract: Provided are a microfluidic apparatus, a method and system for introducing a substance into a cell. The microfluidic apparatus includes a cavity channel, a bulk wave generating device and a surface acoustic wave generating device; a microstructure is arranged on an inner wall of the cavity channel, and the microstructure is constructed for forming a bubble by a solution at the microstructure when the solution is injected into the cavity channel; the bulk wave generating device is configured to generate a bulk wave, the bulk wave enables the bubble to resonate for generating a flow field; and the surface acoustic wave generating device is configured to generate a surface acoustic wave and control a position of at least one particle in the solution.Type: GrantFiled: January 3, 2017Date of Patent: November 29, 2022Assignee: SHENZHEN INSTITUTES OF ADVANCED TECHNOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Hairong Zheng, Wei Zhou, Long Meng, Lili Niu, Zhengrong Lin, Kaiyue Wang, Xiaowei Huang
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Patent number: 11506672Abstract: The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using assays that detect one or more of Follistatin-related protein 3, Basigin, Cathepsin B, and Tenascin as diagnostic and prognostic bio-marker assays in renal injuries.Type: GrantFiled: June 10, 2016Date of Patent: November 22, 2022Assignee: Astute Medical, Inc.Inventors: Joseph Anderberg, Paul McPherson, Jeff Gray, Kevin Nakamura, James Patrick Kampf, Thomas Kwan
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Patent number: 11465144Abstract: In one aspect of the invention, the fluidic device includes a fluidic chip includes a body having a first surface and an opposite, second surface, one or more channels formed in the body in fluidic communications with input ports and output ports for transferring one or more fluids between the input ports and the output ports, and a fluidic chip registration means formed on the first surface for aligning the fluidic chip with a support structure; and an actuator configured to engage with the one or more channels at the second surface of the body for selectively and individually transferring the one or more fluids through the one or more channels from at least one of the input ports to at least one of the output ports at desired flowrates.Type: GrantFiled: August 20, 2019Date of Patent: October 11, 2022Assignee: VANDERBILT UNIVERSITYInventors: David K. Schaffer, Dmitry A. Markov, Ronald S. Reiserer, Lisa McCawley, Michael Geuy, Clayton M. Britt, John P. Wikswo
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Patent number: 11454629Abstract: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.Type: GrantFiled: June 22, 2020Date of Patent: September 27, 2022Assignee: Berkeley Lights, Inc.Inventors: Kristin G. Beaumont, Peter J. Beemiller, Volker L. S. Kurz, Gregory G. Lavieu, Xiaohua Wang, Aathavan Karunakaran
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Patent number: 11446662Abstract: A system for capturing a biomolecule in a single cell includes: a two-dimensional array having single cell capture holes on one surface of a substrate, and biomolecule capture areas inside the substrate each comprising a biomolecule capture member for capturing a biomolecule extracted from individual cells respectively captured by the single cell capture holes; a flow channel for flowing a sample containing cells to be assayed, from a 1st direction parallel to the surface of the substrate; a structure on the surface of the substrate and opposed to the 1st direction on the downstream side of each of the single cell capture holes; a 1st application means applying a 1st flow, and a 1st control means; and a 2nd application means applying a 2nd flow orthogonal to the one surface of the substrate toward each biomolecule capture area from each corresponding single cell capture hole, and a 2nd control means.Type: GrantFiled: March 28, 2016Date of Patent: September 20, 2022Assignee: HITACHI, LTD.Inventors: Tomoyuki Sakai, Masataka Shirai
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Patent number: 11450121Abstract: An optical readout method for detecting a precipitate (e.g., a precipitate generated from the LAMP reaction) contained within a droplet includes generating a plurality of droplets, at least some which have a precipitate contained therein. The droplets are imaged using a brightfield imaging device. The image is subject to image processing using image processing software executed on a computing device. Image processing isolates individual droplets in the image and performs feature detection within the isolated droplets. Keypoints and information related thereto are extracted from the detected features within the isolated droplets. The keypoints are subject to a clustering operation to generate a plurality of visual “words.” The word frequency obtained for each droplet is input into a trained machine learning droplet classifier, wherein the trained machine learning droplet classifier classifies each droplet as positive for the precipitate or negative for the precipitate.Type: GrantFiled: June 26, 2018Date of Patent: September 20, 2022Assignee: The Regents of the University of CaliforniaInventors: Dino Di Carlo, Aydogan Ozcan, Omai B. Garner, Hector E. Munoz, Carson Riche
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Patent number: 11435370Abstract: A sample group forming section 24 classifies samples derived from microorganisms into groups according to empirical information showing the species or strain of each sample. A differential analysis section 27 performs a differential analysis using a peak matrix created based on the result of the grouping. An operator enters group rearrangement conditions concerning the drug resistance of microorganisms. Under the entered conditions, a sample group rearranging/rearrangement-cancelling section 25 rearranges the already formed groups by selecting or merging groups using another kind of previously registered empirical information which shows the drug resistance of each group. The differential analysis section 27 performs a differential analysis using a peak matrix newly created based on the result of the rearrangement of the groups. Thus, differential analysis results concerning the resistance to different drugs can be sequentially acquired as the group rearrangement condition is successively changed.Type: GrantFiled: November 9, 2017Date of Patent: September 6, 2022Assignee: SHIMADZU CORPORATIONInventors: Yoshihiro Yamada, Koretsugu Ogata, Hiroto Tamura, Teruyo Kato
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Patent number: 11414712Abstract: Methods and devices for isolating microbial cells from a sample, extracting eukaryotic DNA from a sample, and identifying the microbial species in the sample are disclosed herein.Type: GrantFiled: September 17, 2015Date of Patent: August 16, 2022Assignee: HELIXBIND, INC.Inventors: Alon Singer, Ranjit Prakash, Srinivas Rapireddy, Jork Nolling
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Patent number: 11408031Abstract: Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.Type: GrantFiled: June 19, 2018Date of Patent: August 9, 2022Assignee: Natera, Inc.Inventors: Allison Ryan, Styrmir Sigurjonsson, Milena Banjevic, George Gemelos, Matthew Hill, Johan Baner, Matthew Rabinowitz, Zachary Demko, Bernhard Zimmerman
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Patent number: 11345960Abstract: Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.Type: GrantFiled: June 19, 2018Date of Patent: May 31, 2022Assignee: Natera, Inc.Inventors: Allison Ryan, Styrmir Sigurjonsson, Milena Banjevic, George Gemelos, Matthew Hill, Johan Baner, Matthew Rabinowitz, Zachary Demko, Bernhard Zimmerman
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Patent number: 11299789Abstract: A method and system are disclosed for detecting microbial pathogens in a sample suspected of containing the pathogens. The method includes combining loop-mediated isothermal amplification (LAMP) reagents and a polymer gel, such as a hydrogel, together with the sample to form a mixture. The gel polymerizes over a short time to immobilize the viral particles within the mixture. If target DNA/RNA are present in the sample, amplicons are produced. The target microorganisms are detected by visually detecting the presence or absence of the amplicons. The target microorganism concentrations may be determined based on the number of fluorescent amplicon dots after the reaction using a smartphone or a fluorescent microscope. The method may be employed for rapidly and inexpensively quantifying microbial pathogens in environmental water samples with high sensitivity.Type: GrantFiled: December 31, 2018Date of Patent: April 12, 2022Assignee: California Institute of TechnologyInventors: Xiao Huang, Michael R. Hoffmann
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Patent number: 11302416Abstract: Systems and methods are disclosed to detect single-nucleotide variations (SNVs) from somatic sources in a cell-free biological sample of a subject by generating training data with class labels; in computer memory, generating a machine learning unit comprising one output for each of adenine (A), cytosine (C), guanine (G), and thymine (T) calls; training the machine learning unit; and applying the machine learning unit to detect the SNVs from somatic sources in the cell-free biological sample of the subject, wherein the cell-free biological sample comprises a mixture of nucleic acid molecules from somatic and germline sources.Type: GrantFiled: September 1, 2016Date of Patent: April 12, 2022Assignee: Guardant HealthInventors: Bahram Ghaffarzadeh Kermani, Helmy Eltoukhy
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Patent number: 11279924Abstract: A method of preparing a conditionally active polypeptide from a parent polypeptide, comprising steps of evolving a DNA encoding the parent polypeptide by increasing a net charge of the parent polypeptide using one or more techniques selected from increasing a total number of codons of charged amino acid residues in the DNA and decreasing a total number of codons of uncharged amino acid residues in the DNA to create mutant DNAs; expressing the mutant DNAs to obtain mutant polypeptides; and selecting the conditionally active polypeptide from the mutant polypeptides which exhibits a decrease in activity in a first assay at a first value of a condition compared to the same activity in a second assay at a second value of the same condition. The conditionally active polypeptide, pharmaceutical compositions containing same, nanoparticle and drug conjugates thereof and uses thereof are also provided.Type: GrantFiled: August 22, 2017Date of Patent: March 22, 2022Assignee: BioAtla, Inc.Inventors: Jay M. Short, Hwai Wen Chang, Gerhard Frey
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Patent number: 11249090Abstract: The invention relates to a method of predicting therapeutic responses to TNF blockers before anti-TNF therapy comprising analyzing immune parameters to selected stimuli in patients before therapy and its use for anti-TNF therapy. The invention relates also to a method of determining a predictive biomarker of response to anti-TNF therapy and to the use of the predictive biomarker obtained by the method.Type: GrantFiled: September 27, 2016Date of Patent: February 15, 2022Assignees: INSTITUT PASTEUR, ASSISTANCE PUBLIQUE—HOPITAUX DE PARIS, UNIVERSITE DE PARISInventors: Lars Rogge, Silvia Menegatti, Maxime Dougados, Elisabetta Bianchi
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Patent number: 11237169Abstract: Compositions, methods and kits are described for identifying biomolecules (e.g., proteins and nucleic acids) expressed in a biological sample that are associated with the presence, development, or progression of a disease (such as cancer), or more generally determination of the etiology or risk factors associated with a disease. Sample types analyzed by the disclosed methods include but are not limited to archival tissue blocks that have been preserved in a fixative, tissue biopsy samples, tissue microarrays, and so forth. The methods disclosed herein correlate expression profiles of biomolecules with various disease types, and allow for the determination of relative survival rates; in some embodiments, the methods permit determination of survival rates for a subject with cancer. In other embodiments, the disclosure relates to methods for evaluating therapeutic regimes for the treatment, such as treatment of cancer.Type: GrantFiled: December 12, 2018Date of Patent: February 1, 2022Assignee: The United States of America, as represented by the Secretary, Department of Health and Human ServicesInventors: Stephen M. Hewitt, Joon-Yong Chung
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Patent number: 11207685Abstract: Systems, methods, and devices for forming an array of emulsions. An exemplary device comprises a frame and at least one or a plurality of separate microfluidic modules mounted to the frame and each configured to form an array of emulsions. In some embodiments, each module may be mounted by snap-fit attachment. The device also may include the same sealing member bonded to a top side of each module and hermetically sealing each of the modules. Another exemplary microfluidic device for forming an array of emulsions comprises a stack of layers bonded together. The stack may comprise a port layer forming a plurality of ports. Each port may have a top rim formed by a protrusion that encircles the central axis of the port. The rims may be coplanar with one another to facilitate bonding of a sealing member to each rim.Type: GrantFiled: February 12, 2018Date of Patent: December 28, 2021Assignee: Bio-Rad Laboratories, Inc.Inventors: Thomas Henry Cauley, III, John Dzenitis, Steve Hobbs, Stefano Schiaffino
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Patent number: 11206138Abstract: An apparatus includes a tester to detect a biological signature of a biological sample, a processor, and a memory operably coupled to the processor. The memory stores instructions to cause the processor to receive an indication of the biological signature from the tester, and to generate, using a smart contract and through communication with a distributed ledger, a cryptographic token including a digital identifier based on the biological signature. The cryptographic token is transmitted to a remote processor for verification of the biological sample, in response to receiving the cryptographic token. The tester can detect the biological signature within a predetermined test duration that is less than a DNA sequencing duration associated with the biological sample, and the biological signature has a data precision sufficient to uniquely identify the biological sample from a plurality of biological samples.Type: GrantFiled: October 16, 2019Date of Patent: December 21, 2021Assignees: Ernst & Young U.S. LLP, EYGS LLPInventors: James C. Canterbury, Shyam Bharat Khatau, Parvez Hakim
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Patent number: 11198912Abstract: Embodiments of the invention include a system and method of using biomarkers in the diagnosis of lung cancer. A subject can be screened for lung cancer based on expression of specific mRNAs, miRNAs or the detection of a nucleic acid, protein, peptide or other biological molecule in blood, serum or plasma. Embodiments include 29 specific miRNAs for use as biomarkers to screen or distinguish healthy individuals from individuals affected with the disease, including a lung cancer, along with 81 additional nucleic acids, proteins or peptides for use as biomarkers to screen or distinguish healthy individuals from individuals affected with the disease, including a lung cancer. Levels of more than one of the mRNAs, miRNAs or proteins can be scored and compared to one or more threshold values to diagnose or determine the prognosis of lung cancer. Embodiments also include a kit for screening healthy subjects from subjects affected with the disease.Type: GrantFiled: August 26, 2020Date of Patent: December 14, 2021Assignee: Liquid Lung DXInventors: Patrick Lilley, Martin J. Keiser, III
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Patent number: 11142793Abstract: Sequencing methods, devices, and systems are described. Arrays of nanoscale electronic elements comprising two electrodes separated by an insulating layer are used to provide sequence information about a template nucleic acid in a polymerase-template complex bound proximate to the insulating region. A sequencing reaction mixture comprising nucleotide analogs having impedance labels is introduced to the array of nanoscale electronic elements under conditions of polymerase mediated nucleic acid synthesis. The time sequence of incorporation of nucleotide analogs is determined by identifying the types of labels of the nucleotide analogs that are incorporated into the growing strand using measured impedance.Type: GrantFiled: October 30, 2019Date of Patent: October 12, 2021Assignee: Pacific Biosciences of California, Inc.Inventors: Stephen Turner, Jeremiah Hanes, Keith Bjornson
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Patent number: 11111531Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.Type: GrantFiled: July 11, 2019Date of Patent: September 7, 2021Assignee: TANGEN BIOSCIENCES, INC.Inventors: John R. Nobile, John Davidson
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Patent number: 11104950Abstract: Panels of 8-, 9- and 12-biomarker for diagnostic and prognostic methods to determine a subject's radiation exposure and discriminates between persons who have been exposed to radiation only, inflammation stress only, or a combination of the two.Type: GrantFiled: November 7, 2014Date of Patent: August 31, 2021Assignee: The Regents of the University of CaliforniaInventors: Andrew J. Wyrobek, Antoine M. Snijders
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Patent number: 11097247Abstract: Methods of generating a nucleic acid signature for identifying particles associated in a partition are provided. In one aspect, the method comprises: partitioning a sample into a plurality of partitions comprising a particle comprising a solid support surface, the solid support surface having a plurality of oligonucleotide primers conjugated thereon, wherein the oligonucleotide primers comprise a barcode sequence, and wherein the partitions have 0, 1, or more than 1 particles per partition; providing in a partition a substrate comprising a barcode sequence or repeating clonal barcode sequences; and in the partition, associating a first particle conjugated to oligonucleotide primers comprising a first barcode sequence and a second particle conjugated to oligonucleotide primers comprising a second barcode sequence to a barcode sequence from the substrate, thereby generating a nucleic acid signature for the particles in the partition.Type: GrantFiled: June 25, 2020Date of Patent: August 24, 2021Assignee: Bio-Rad Laboratories, Inc.Inventors: Ronald Lebofsky, Nicholas Heredia
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Patent number: 11078543Abstract: Provided herein is technology for pancreatic high-grade dysplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of pancreatic high-grade dysplasia (IPMN-HGD, PanIN-3, or PDAC).Type: GrantFiled: April 13, 2017Date of Patent: August 3, 2021Assignee: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCHInventors: David A. Ahlquist, William R. Taylor, John B. Kisiel, Tracy C. Yab, Douglas W. Mahoney
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Patent number: 11072826Abstract: Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.Type: GrantFiled: June 19, 2018Date of Patent: July 27, 2021Assignee: Natera, Inc.Inventors: Allison Ryan, Styrmir Sigurjonsson, Milena Banjevic, George Gemelos, Matthew Hill, Johan Baner, Matthew Rabinowitz, Zachary Demko
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Patent number: 11061045Abstract: A matrix droplet extruder includes one or a plurality of reagent containers. Pneumatic connectors are each connectable to a corresponding docking station connector of a docking station that is connectable to a pneumatic generator controllable by a controller to provide pneumatic pressure to the pneumatic docking station connectors. A droplet matrix extrusion surface includes an array of perforations. A liquid management chip has a network of dispensing channels for dispensing reagents from reagent containers through the array of perforations. A pneumatic control network includes pneumatic channels and gates that are controllable by application of the pneumatic pressure to the gates via the pneumatic channels to enable or block dispensing the one or more reagents to repeatedly generate a matrix of droplets when applying the pneumatic pressure to the reagents.Type: GrantFiled: March 12, 2019Date of Patent: July 13, 2021Assignee: Picodya Technologies Ltd.Inventor: Yehuda Yavets-Chen
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Patent number: 11047013Abstract: Provided herein is technology for pancreatic high-grade dysplasia screening and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of pancreatic high-grade dysplasia (IPMN-HGD, PanIN-3, or PDAC).Type: GrantFiled: April 13, 2017Date of Patent: June 29, 2021Assignee: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCHInventors: David A. Ahlquist, William R. Taylor, John B. Kisiel, Tracy C. Yab, Douglas W. Mahoney
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Patent number: 11021757Abstract: There is a need for improved methods for determining the diagnosis and prognosis of patients with conditions, including autoimmune disease and cancer, especially lymphoid neoplasms, such as lymphomas and leukemias. Provided herein are methods for using DNA sequencing to identify personalized, or patient-specific biomarkers in patients with lymphoid neoplasms, autoimmune disease and other conditions. Identified biomarkers can be used to determine and/or monitor the disease state for a subject with an associated lymphoid disorder or autoimmune disease or other condition. In particular, the invention provides a sensitive method for monitoring lymphoid neoplasms that undergo clonal evolutions without the need to development alternative assays for the evolved or mutated clones serving as patient-specific biomarkers.Type: GrantFiled: December 13, 2018Date of Patent: June 1, 2021Assignee: Adaptive Biotechnologies CorporationInventors: Malek Faham, Thomas Willis
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Patent number: 11016082Abstract: Provided is a non-invasive method of detecting or screening for a cancer in a subject specimen originating in a tissue outside of the lung. The method includes detecting elevated levels of one or more carbonyl-containing volatile organic compounds (VOCs) that are biomarkers for the cancer in exhaled breath from the subject specimen. The method may further include obtaining exhaled breath from the subject specimen; forming adducts of the carbonyl-containing VOCs with a reactive chemical compound; quantifying the adducts of the carbonyl-containing VOCs to establish a subject value for each of the adducts; and comparing each subject value to a threshold healthy specimen value for each of the adducts of the carbonyl-containing VOCs. One or more subject values at quantities greater than threshold healthy specimen values are also useful for screening for the cancer in the subject specimen.Type: GrantFiled: July 29, 2016Date of Patent: May 25, 2021Assignee: University of Louisville Research Foundation, Inc.Inventors: Xiaoan Fu, Michael Nantz, Michael Bousamra, Victor van Berkel
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Patent number: 10994275Abstract: A system for processing a plurality of biological samples contains a support and a temperature controller. The suppose is configured to hold a case that includes an inner chamber and a substrate located within the inner chamber, the substrate containing a plurality of isolated reaction sites containing one or more biological samples. The temperature controller is configured to maintain or control a temperature of at least one of the support, the case, or the one or more biological samples during an assay or reaction on the one or more biological samples. The support is also configured to maintain at least one of the surfaces of substrate at a positive angle relative to a horizontal plane during the assay or reaction.Type: GrantFiled: March 4, 2019Date of Patent: May 4, 2021Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Michael Pallas, Evan Foster, Jorge Fonseca, James Nurse, Theodore Straub
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Patent number: 10964409Abstract: Provided herein are methods, processes, systems and machines for non-invasive assessment of genetic variations.Type: GrantFiled: October 2, 2014Date of Patent: March 30, 2021Assignee: Sequenom, Inc.Inventor: Gregory Hannum
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Patent number: 10961583Abstract: The disclosure provides methods of identifying a human subject as a candidate for treating or inhibiting a liver disease by inhibiting HSD17B13. The disclosure also provides methods of treating a subject who is PNPLA3 Ile148Met+ by administering an inhibitor of HSD17B13. The disclosure also provides method of detecting a PNPLA3 Ile148Met variant and functional HSD17B13 in a subject. The disclosure also provides method of identifying a subject having a protective effect against liver disease. The disclosure also provides inhibitors of HSD17B13 for use in the treatment of a liver disease.Type: GrantFiled: October 11, 2018Date of Patent: March 30, 2021Assignee: Regeneron Phramaceuticals, Inc.Inventors: Yurong Xin, Jesper Gromada, Xiping Cheng, Frederick Dewey, Tanya Teslovich Dostal, Claudia Schurmann, Aris Baras, Noura Abul-Husn
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Patent number: 10956806Abstract: A computer-implemented method for efficient assembly of oligonucleotides for nucleic acid based data storage includes receiving encoded data including binary data encoded into nucleic acid sequence data, and assembling a target nucleic acid data strand based on the encoded data by, concatenating one or more selected codeword oligonucleotides obtained from a codeword stack strand.Type: GrantFiled: June 10, 2019Date of Patent: March 23, 2021Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATIONInventors: Koji Masuda, Eiji Nakamura
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Patent number: 10907196Abstract: The present application relates to new coumarin compounds and their uses as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.Type: GrantFiled: January 9, 2020Date of Patent: February 2, 2021Assignee: Illumina Cambridge LimitedInventor: Nikolai Nikolaevich Romanov