Abstract: The present invention provides a method for rapidly clearing a biological tissue. According to the present invention, provided is a method for clearing a biological tissue, including the steps of: infiltrating the biological tissue with water-soluble ethylenically unsaturated monomers before, during or after fixing the biological tissue with a fixative, wherein the water-soluble ethylenically unsaturated monomers include at least a water-soluble ethylenically unsaturated monomer having an ionically dissociable group; polymerizing the water-soluble ethylenically unsaturated monomers to form a hydrogel in the fixed biological tissue; and removing a lipid from the fixed biological tissue.

Abstract: A composition, kit and method useful in a multi-stage washing method for cleaning an aluminum or aluminum alloy containers while reducing hazardous exposure of workers to toxic levels of hydrogen fluoride or ammonium bifluoride employs a stable, neutralized ammonium bifluoride-containing accelerator solution for addition to an acidic wash solution.

Abstract: The invention relates to a lipase containing water-soluble film, and a detergent pouch formed by the water-soluble film.

Abstract: The invention relates to a cleaning composition comprising a lipase with at least 75% identity to SEQ ID NO: 2, and a surfactant, wherein said composition is more effective in removing lipid stains present at a surface in comparison with an equivalent composition lacking the lipase.

Abstract: Liquid compositions for cleaning, in particular medical instruments and air conditioning surfaces, said composition excluding surfactants and comprising one or more enzymes including a protease and optionally a hydrolase, a solvent system including a water soluble glycol ether solvent, at least one anionic hydrotrope, and wherein the molar ratio of said at least one hydrotrope to said glycol ether in the composition is selected to preserve the activity of said one or more enzymes. The hydrotrope is advantageously an anionic hydrotrope selected from the group consisting of water soluble anionic hydrotropes of the formula (I) and having no alkyl side chain greater than six carbons in length, for example a xylene sulfonate or cumene sulfonate salt.

Abstract: A liquid detergent composition containing (A) 10 to 70 mass % of a nonionic surfactant, (B) 1 to 15 mass % of an anionic surfactant, (C) 0.01 to 2 mass % of a protease, and (D) 0.001 to 0.1 mass % of at least one compound selected from the group consisting of thiazole-based compounds and sulfur-containing amino acids.

Abstract: A detergent composition comprising a polypeptide having protease activity is disclosed. The composition may be used in laundry or hard surface cleaning such as automated dish wash, and it has improved performance compared with a commercial protease.

Abstract: The present disclosure relates to benefit agent delivery particles containing at least one benefit agent and at least one cellulosic polymer. The disclosure further relates to compositions containing benefit agent delivery particles and processes for making and using such compositions. The disclosure further relates to methods of imparting a benefit delivery capability to a cleaning composition.

Abstract: The present invention relates to variants of a parent ?-amylase, which parent ?-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No.

Abstract: The present invention provides methods for protein engineering and serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.

Abstract: A method of laundering fabric including the step of contacting a liquid laundry detergent composition to water to form a wash liquor, and laundering fabric in the wash liquor, wherein the laundry detergent is contacted to water in such an amount so that the concentration of the laundry detergent composition in the wash liquor is from above 0 g/l to 4 g/l, and wherein from 0.01 kg to 2 kg of fabric per liter of wash liquor is dosed into the wash liquor, and wherein the liquid laundry detergent composition comprises: (i) detersive surfactant; (ii) surfactancy boosting polymer; (iii) from 0 wt % to 10 wt % fatty acid; (iv) silicone suds suppressor; (v) structurant; (vi) enzyme; and (vii) nil-boron enzyme stabilizer; wherein the electrolytic strength of the composition at a concentration of 1 g/l in de-ionized water and at a temperature of 25° C. in mScm?1 is less than 200 mScm?1.

Abstract: A drain cleaner may be used on a periodic basis to clean soil residues from residential and commercial waste drains. The drain cleaner may chemically self-foam to fill a waste drain with the foam. For example, the drain cleaner may be provided in two or more parts that are physically intermixed during use of the drain cleaner. One part may include hydrogen peroxide and water while another part may include a catalase, an amylase, a protease, and an enzyme stabilizer. The drain cleaner may also include a surfactant present in at least one of the first part and the second part. Additionally, in some examples, the drain cleaner includes a sanitizing agent present in either of the two parts or in yet a third physically separate part. During use, the different drain cleaner parts can be dispensed simultaneously into a drain to generate a cleaning and/or sanitizing foam in-situ.

Abstract: The conversion of a peptide aldehyde to a hydrosulfite adduct can be used to increase the aqueous solubility in the purification of the peptide aldehydes. Advantageously, this hydrosulfite adduct is itself effective as a subtilisin inhibitor and stabilizer and it can also stabilize a second enzyme if present. The hydrosulfite adduct is effective as a subtilisin inhibitor, and it maintains its inhibitory and stabilizing effect in a liquid detergent during storage. Thus, use of the hydrosulfite adduct can avoid the cost and time of converting it back to the peptide aldehyde and subsequent drying of the peptide aldehyde can be saved, and this can avoid the inconvenience of handling the peptide aldehyde in powder form or as a highly diluted aqueous solution.

Abstract: A hydrolytic enzyme is stabilized in a liquid surfactant preparation using a component that stabilizes the hydrolytic enzyme and includes a phenylalkyldicarboxylic acid.

Abstract: A hydrolytic enzyme is stabilized in a liquid surfactant preparation using a component that stabilizes the hydrolytic enzyme and encompasses an oligoaminobiphenyl oligocarboxylic acid.

Abstract: A drain cleaner may be used on a periodic basis to clean soil residues from residential and commercial waste drains. The drain cleaner may chemically self-foam to fill a waste drain with the foam. For example, the drain cleaner may be provided in two or more parts that are physically intermixed during use of the drain cleaner. One part may include hydrogen peroxide and water while another part may include a catalase, an amylase, a protease, and an enzyme stabilizer. The drain cleaner may also include a surfactant present in at least one of the first part and the second part. Additionally, in some examples, the drain cleaner includes a sanitizing agent present in either of the two parts or in yet a third physically separate part. During use, the different drain cleaner parts can be dispensed simultaneously into a drain to generate a cleaning and/or sanitizing foam in-situ.

Abstract: The intention is to improve storage stability in a liquid washing or cleaning agent which contains a protease and amylase. This is achieved by using a protease which comprises an amino acid sequence which, over the entire length thereof, is at least 70% identical to the amino acid sequence stated in SEQ ID no. 1 and, in the numbering according to SEQ ID no. 1, has the amino acid substitution R99E or R99D in combination with at least two further amino acid substitutions which are selected from the group consisting of S3T, V4I and V199I.

Abstract: The present disclosure relates to benefit agent delivery particles containing at least one benefit agent and at least one cellulosic polymer. The disclosure further relates to compositions containing benefit agent delivery particles and processes for making and using such compositions. The disclosure further relates to methods of imparting a benefit delivery capability to a cleaning composition.

Abstract: A method of making a Bacillus alpha-amylase variant that increases alpha-amylase production and the alpha-amylases produced thereby. The recombinant alpha-amylases can be placed in compositions and used for purposes of laundry detergents, cleaning and dishwashing detergents, fabric desizing, starch liquefaction, cereal liquefaction, starch saccharification, biofilm removal, and starch hydrolysis in cane sugar processing.

Abstract: A braided hair washing method includes washing braided hair using a washing agent that includes a microbubble washing composition and microbubbles, the microbubble washing composition including a protease and a lipase. The braided hair washing method can conveniently and effectively wash braided hair without collapsing the hairstyle.

Abstract: In a liquid detergent comprising a subtilisin and optionally a second (non-subtilisin) enzyme, the combination of a peptide aldehyde (or hydrosulfite adduct thereof) with a salt of a monovalent cation and a monovalent organic anion has a synergistic stabilizing effect on the subtilisin and/or the second enzyme. The improved enzyme stability is of particular interest in liquid detergent compositions where the enzyme would otherwise have poor storage stability.

Abstract: The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions for generation of peracids. The present invention finds particular use in applications involving cleaning, bleaching and disinfecting.

Abstract: A hard floor surface care process comprising a process of identifying, cleaning, polishing, and protecting manmade and natural stone hard floor surfaces having a single surface or multi-surface quality. The hard floor surface care process comprising an acid reactive or nonreactive hard floor surface identifying process; an emulsifying solution, agitating, and toweling cleaning process; a polishing process utilizing a lubricating solution with a polishing chemistry or pad, and a protecting process utilizing a protecting chemistry selected as a function of the identifying process.

Abstract: A method of making an aqueous ozone solution for an industrial cleaning system is described. The method includes providing a reaction vessel for entraining ozone gas in an aqueous solution. The reaction vessel includes a conical-shaped surface having a two or more edges or ridges. The conical-shaped surface defines an interior, and two or more edges or ridges are in contact with the interior. The reaction vessel is in fluidic communication with a supply of water. The reaction vessel is in fluidic communication with a supply of a first aqueous ozone solution. The first aqueous ozone solution is directed to the conical-shaped surface. Water is directed to the conical-shaped surface, and the water and the first aqueous ozone solution are mixed to form a second aqueous ozone solution.

Abstract: Surfactant-containing compositions are described which include a protein component that has the effect of improving the surface-active properties of the surfactants contained in the compositions. The surfactant-containing compositions having the protein component demonstrate significantly lower critical micelle concentrations (CMC), reduced surface tensions, and reduced interfacial tensions than do comparable compositions having no protein component. In addition, the surfactant-containing compositions having the protein component has the effect of converting greasy waste contaminants to surface active materials.

Abstract: The present invention provides methods and compositions comprising at least one thermolysin-like neutral protease enzyme with improved storage stability and/or catalytic activity. In some embodiments, the thermolysin finds use in cleaning and other applications comprising detergent. In some particularly preferred embodiments, the present invention provides methods and compositions comprising thermolysin formulated and/or engineered to resist detergent-induced inactivation.

Abstract: Treatment composition, preferably liquid detergent compositions exhibiting a two, or multicolor effect, comprising an aesthetic system including a colored interference pigment and a dye system.

Abstract: The present invention relates to aqueous liquid detergent compositions comprising an alkaline protease and/or one or more other non-proteolytic enzymes, and an enzyme stabilization system. The invention also relates to enhancing stability of the non-proteolytic enzymes in a liquid detergent composition comprising an alkaline protease.

Abstract: Liquid stable enzyme compositions and methods of employing the same for cleaning, including warewashing and dishwashing, are disclosed. The stable enzyme compositions preferably employ an amphoteric surfactant stabilizing agent, such as disodium camphodiacetate (CADA), to stabilize a mixture of traditionally unstable enzymes, such as proteases and lipases.

Abstract: The present compositions and methods relate to an endo-?-mannanase cloned from Bacillus agaradhaerens, polynucleotides encoding the endo-?-mannanase, and methods of use thereof. Formulations containing the endo-?-mannanase are highly suitable for use as detergents.

Abstract: The present compositions and methods relate to an endo-B-mannanase cloned from a Bacillus sp., polynucleotides encoding the endo-B-mannanase, and methods of use thereof. Formulations containing the endo-?-mannanase are highly suitable for use as detergents.

Abstract: According to the invention, storage stability in terms of amylolytic activity is to be improved in a liquid washing or cleaning agent which comprises a protease and amylase. This is achieved by the use of a protease which comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO. 1 and which has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the count according to SEQ ID NO. 1.

Abstract: The present invention relates to bacteria cultures and composition comprising one or more cultures of the invention. The invention also relates to methods of washing or cleaning laundry or fabrics and surfaces as well as degrading waste material using a bacteria culture of the invention.

Abstract: The present invention provides novel Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.

Abstract: According to the invention, storage stability in terms of cellulolytic activity is to be improved in a liquid washing or cleaning agent which comprises a protease and cellulase. This is achieved by the use of a protease which comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO. 1 and which has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the count according to SEQ ID NO. 1.

Abstract: A hard floor surface care process comprising a process of identifying, cleaning, polishing, and protecting manmade and natural stone hard floor surfaces having a single surface or multi-surface quality. The hard floor surface care process comprising an acid reactive or nonreactive hard floor surface identifying process; an emulsifying solution, agitating, and toweling cleaning process; a polishing process utilizing a lubricating solution with a polishing chemistry or pad, and a protecting process utilizing a protecting chemistry selected as a function of the identifying process.

Abstract: The aim of the invention is to stabilize a hydrolytic enzyme in a liquid surfactant preparation. Said aim is achieved by using a component stabilizing the hydrolytic enzyme, said component comprising boric acid, propylene glycol and a phenylboronic acid derivative having the structural formula (I) in which R is hydrogen, a hydroxyl group, a C1-C6 alkyl group, a substituted C1-C6 alkyl group, a C1-C6 alkenyl group or a substituted C1-C6 alkenyl group.

Abstract: The present application relates to washing and cleaning agents, containing phosphate compounds having aliphatic and/or aromatic residues, which act as enzyme stabilizers. Further subjects are the use of such compounds as reversible inhibitors of enzymes, in particular of proteolytic enzymes, and thus as stabilizers in washing or cleaning agents, and further methods and uses correlated therewith.

Abstract: A hydrolytic enzyme is to be stabilized in a liquid surfactant preparation. This is achieved by using a component that stabilizes the hydrolytic enzyme and encompasses a phthaloylglutamic acid and/or a phthaloylaspartic acid.

Abstract: A hydrolytic enzyme is stabilized in a liquid surfactant preparation by using a component that stabilizes the hydrolytic enzyme and includes a monosaccharide glycerate.

Abstract: A hydrolytic enzyme is stabilized in a liquid surfactant preparation using a component that stabilizes the hydrolytic enzyme and includes a phenylalkyldicarboxylic acid.

Abstract: A hydrolytic enzyme is stabilized in a liquid surfactant preparation using a component that stabilizes the hydrolytic enzyme and encompasses an oligoaminobiphenyl oligocarboxylic acid.

Abstract: A hydrolytic enzyme is to be stabilized in a liquid surfactant preparation. This is achieved by using a component that stabilizes the hydrolytic enzyme and encompasses a multiply substituted benzenecarboxylic acid that has a carboxyl group on at least two carbon atoms of the benzene residue.

Abstract: A non-toxic enzymatic detergent for use in cleaning surgical instruments and the like which have a large build-up of bio-residue thereon and which need specialty cleaning in order to avoid both hardened bio-residue and certain fluid traces from the instruments for both operating accuracy as well as sterilization requirements.

Abstract: The need for a means to formulate fluid compositions for use in water-soluble unit dose articles having higher levels of water is met by incorporating a di-amido gellant into the fluid composition. The di-amido gellant also simplifies the addition of ingredients to water-soluble unit dose articles, such as microcapsules, that are typically added as aqueous suspensions or slurries.

Abstract: A composition including the fermentation supernatant from a fermentation of yeast is intended to be conveniently introduced through the wastewater plumbing system of a private home or other facility into a septic system servicing the home or other facility to substantially accelerate the ability of the bacteria resident in the septic system to substantially digest biologically available organic compounds present in the septic system, and methods of accomplishing the same.

Abstract: A concentrated bioenzymatic cleaning product is provided which maintains a homogenous composition under static conditions and does not require shaking or mixing prior to dilution. The concentrated bioenzymatic cleaning product includes bacillus spores, a suspending and/or rheology modifying agent, an odor control agent, a pH adjuster, and water. The types and amounts of ingredients are selected to maintain the bacillus spores in suspension under static conditions, enabling the concentrated bioenzymatic cleaning product to be diluted using a proportioner system.

Abstract: The present invention relates to bacteria cultures and composition comprising one or more cultures of the invention. The invention also relates to methods of washing or cleaning laundry or fabrics and surfaces as well as degrading waste material using a bacteria culture of the invention.

Abstract: This invention is directed to a water soluble sachet comprising a detergent composition having a discrete particle that enhances cleaning in a dishwashing machine. The water soluble sachet unexpectedly results in excellent cleaning properties and minimizes spot and film formation on items being cleaned in a dishwasher.

Abstract: The present invention relates to cleaning compositions comprising protease-sensitive components, anionic surfactant and protease enzyme, and also incorporating a reversible stabilizer for the protease enzyme and methods for making such compositions.