Alcohol Patents (Class 530/424)
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Patent number: 6433146Abstract: The invention is a corn product removal process that successfully extracts oil and zein from dry-milled corn. Oils and zein are extracted from corn using ethanol. Corn solids are separated from the ethanol, oil and zein mixture produced in the step of extracting. Thereafter, the ethanol, oil and zein mixture are membrane filtered to restrain zein from the mixture and pass an oil and ethanol mixture. At least one of zein or oil is then selected to be separated for an output corn product.Type: GrantFiled: May 18, 1999Date of Patent: August 13, 2002Assignee: The Board of Trustees of the University of IllinoisInventor: Munir Cheryan
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Patent number: 6407214Abstract: This invention relates to whole antibodies of neutral isotype having specificity for E-selection, process for their preparation (using vectors), pharmaceutical compositions containing them, and their use in therapy (e.g. for inflammatory disorders) and diagnosis. Said antibodies are variants of natural antibodies altered in the Fc region, especially in the CH2 domain, so that the interactions with antibodies Fc receptors and complement are absent or very low.Type: GrantFiled: June 5, 2000Date of Patent: June 18, 2002Assignee: Celltech Therapeutics LimitedInventors: Raymond John Owens, Martyn Kim Robinson
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Patent number: 6342367Abstract: Disclosed is an economical method for the preparation of chondroitin sulfates A and C useful as an effective ingredient of medicaments from fish scales as a waste material discharged from fishery in large quantities. Fish scales are enzymatically decomposed in an aqueous medium in the presence of a protease to isolate the chondroitin sulfate compounds and by-product polypeptides followed by removal of the by-product polypeptides from the aqueous solution by a cation-exchange treatment and then the aqueous solution of the chondroitin sulfate compounds is subjected to fractional precipitation by the addition of ethyl alcohol as the precipitant.Type: GrantFiled: February 25, 2000Date of Patent: January 29, 2002Assignee: Japan as represented by Secretary of Agency of Industrial Science and TechnologyInventors: Toshihisa Sumi, Hideki Ohba, Toru Ikegami, Masao Shibata, Tsuyoshi Sakaki, Imre Sallay, Sung Soo Park
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Patent number: 6291654Abstract: This invention relates to the identification of a human complement C3 binding protein from Streptococcus pneumoniae and to its sequence and to methods for its purification and use. The protein binds but does not degrade or cleave C3 and is implicated in S. pneumoniae virulence. The protein is recognized by antibodies produced by humans recovering from pneumococcal infection.Type: GrantFiled: May 12, 1999Date of Patent: September 18, 2001Assignee: Regents of the University of MinnesotaInventors: Margaret K. Hostetter, Qi Cheng
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Patent number: 6162905Abstract: A new process, particularly simple and economical, for FSH and LH separation and purification starting from crude HMG preferably urinary, comprising the following steps:1) optional exhaustion of crude HMG viral charge in aqueous EtOH2) ion-exchange chromatography on weakly basic anionic resins of DEAE type;3) affinity chromatography on resin having an antraquinone derivative as a ligand;4) optional ion-exchange chromatography on strongly basic anionic resins;Hormones obtained thereby, in particularly pure form and having high specific activity, may subsequently undergo a depyrogenation step.Type: GrantFiled: July 6, 1998Date of Patent: December 19, 2000Assignee: IBSA Institut Biochimique S.A.Inventors: Paolo Lualdi, Elisabetta Donati, Irina Rapaport
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Patent number: 6107467Abstract: The present invention relates to a process for purifying a hydrophobic or amphiphilic compound, by first mixing a starting material containing the hydrophobic or amphiphilic compound with a first polymeric material, water and at least one of a second polymeric material and a surfactant, wherein the first polymeric material and the second polymeric material and/or surfactant are immiscible in the resulting primary aqueous solution. The process further comprises maintaining the primary aqueous solution for a period of time sufficient for essentially separating the phases formed, and then removing the phase containing the main portion of the hydrophobic or amphiphilic compound and the second polymeric material and/or surfactant. The second polymeric material and/or surfactant are separated from the hydrophobic or amphiphilic compound, and subsequently recycled to the initial mixing step.Type: GrantFiled: September 5, 1997Date of Patent: August 22, 2000Assignee: Pharmacia & Upjohn ABInventors: Hans Ageland, Lena Nystrom, Josefine Persson, Folke Tjerneld
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Patent number: 6080844Abstract: The present invention involves a process of purifying and recovering a recombinant protein from a suspension of cells. The process of the present invention involves extracting a recombinant protein from a concentrated suspension of cells using a water-miscible organic solvent, isolating the recombinant protein from the suspension of cells, concentrating the recombinant protein to remove the water-miscible organic solvent, precipitating the recombinant protein using an acid, washing the recombinant protein with the salt or free form of a suitable organic acid and recovering the recombinant protein.Type: GrantFiled: April 23, 1998Date of Patent: June 27, 2000Assignee: Abbott LaboratoriesInventors: Ronald E. Carney, Joseph Arndt, Julie R. List, Ellen Marie Schwartz
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Method for immunological determination of hemoglobin derivative and treating reagent for use therein
Patent number: 6027907Abstract: A hemoglobin derivative-containing sample is treated with a treating reagent containing 2-butanol and then immunologically analyzed to determine the quantity of the hemoglobin derivative. By the treatment with 2-butanol, the immunological determination for the hemoglobin derivative, particularly HbA.sub.1c, can be attained with high sensitivity by a simple procedure. Since 2-butanol-containing treating reagent does not affect the enzymatic activity, the homogeneous enzyme immunoassay with high sensitivity is realized.Type: GrantFiled: December 16, 1996Date of Patent: February 22, 2000Assignee: Fuji Photo Film Co., Ltd.Inventors: Hiroshi Shinoki, Yoshikazu Amano -
Patent number: 6019999Abstract: Described is a process for making a liposomal, ion-exchange whey protein and products thereof, which result in the sustained release of amino acids into the body's circulation to generally promote skeletal muscle protein synthesis, decrease body fat in association with diet modification and improve exercise performance. The whey protein is preferably encapsulated in a liposome using a cold, or non-heated, process. After the liposomal, ion-exchange whey protein has been prepared, it is then preferably lyophilized to deliver macronutrients for use as a sports nutrition supplement and for use in medical or clinical catabolic applications.Type: GrantFiled: December 2, 1998Date of Patent: February 1, 2000Inventors: David F. Miller, Ian Hicks, Charles B. Quick, Joey Antonio, Ian J. Reynolds, David Rush, S. Keith Klein, IV
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Patent number: 5855902Abstract: A subunit vaccine for Tritrichomonas foetus and method for preparing such vaccine for use in immunizing and treating animals is provided. The method disclosed involves separating out the antigens by centrifuging homogenized Tritrichomonas foetus cells, preferably at about 830.times.g for about 15 minutes, solubilizing the antigen with an nonionic detergent and completing with saponin. Topical administration, such as intravaginal or intrapretutial administration, of such vaccine preparation in conjunction with subcutaneous administration in combination with other adjuvants is effective to eliminate infection.Type: GrantFiled: February 7, 1994Date of Patent: January 5, 1999Assignee: Protatek International, Inc.Inventors: Miodrag Ristic, Lionel Wanduragala
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Patent number: 5851301Abstract: Methods for separation of wheat flour into protein and starch fractions are described. Wheat flour is (1) mixed with water to hydrate the flour and form a cohesive batter or dough, (2) chilled, and (3) mixed and washed with chilled ethanol to separate it into protein and starch fractions. Wheat protein fractions that are equivalent in yield and protein concentration to fractions produced by water washing methods are obtained, while reducing water and energy use. The protein fraction showed improved dough strength.Type: GrantFiled: June 20, 1997Date of Patent: December 22, 1998Assignee: The United States of America as represented by the Secretary of AgricultureInventors: George H. Robertson, Trung K. Cao
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Patent number: 5814314Abstract: A biologically active, non-glycosylated recombinant human Interleukin 2 (R-hIL.sub.2) in reduced form, process for its preparation and method of use.Type: GrantFiled: February 12, 1996Date of Patent: September 29, 1998Assignee: Roussel UclafInventors: Danielle Lando, Philippe Riberon, Pierre Yves Abecassis
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Patent number: 5808006Abstract: A composition is provided comprising about 0.1 to 15 mg/ml of a polypeptide in a buffer having a pH of about 7-12 comprising about 5-40% (v/v) of an alcoholic or polar aprotic solvent, about 0.2 to 3M of an alkaline earth, alkali metal, or ammonium salt, about 0.1 to 9M of a chaotropic agent, and about 0.01 to 15 .mu.M of a copper or manganese salt. The buffer is suitably used in a method for refolding improperly folded polypeptides.Type: GrantFiled: October 11, 1994Date of Patent: September 15, 1998Assignee: Genentech, Inc.Inventors: Stuart Builder, Roger Hart, Philip Lester, David Reifsnyder
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Patent number: 5798446Abstract: In the method described, a protein-containing substance is first taken up in an alkaline solvent to give a solution. Insoluble constituents of the substance are separated off, the solution is neutralized and desalinated, and then the proteins contained in the solution are concentrated. The solubilization or disintegration of the protein-containing substance is carried out at room temperature using homogenization equipment. The heat dissipated into the protein-containing substance during homogenization is simultaneously removed. The pH of the alkaline solvent during the decomposition is over 11.5 and/or decomposition is carried out in the presence of a detergent, in particular sodium dodecylsulfate (SDS).Type: GrantFiled: October 16, 1996Date of Patent: August 25, 1998Assignee: Nupron GmbH ProteinwerkInventor: Waldemar Neumuller
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Patent number: 5756672Abstract: A composition is provided comprising about 0.1 to 15 mg/mL of a polypeptide in a buffer having a pH of about 7-12 comprising about 5-40% (v/v) of an alcoholic or polar aprotic solvent, about 0.2 to 3M of an alkaline earth, alkali metal, or ammonium salt, about 0.1 to 9M of a chaotropic agent, and about 0.01 to 15 .mu.M of a copper or manganese salt. The buffer is suitably used in a method for refolding improperly folded polypeptides.Type: GrantFiled: June 6, 1995Date of Patent: May 26, 1998Assignee: Genentech, Inc.Inventors: Stuart Builder, Roger Hart, Philip Lester, David Reifsnyder
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Patent number: 5734026Abstract: A process for manufacturing crystals of growth hormone (GH) comprising the steps of:i) mixing GH with an aqueous solution comprising a buffer and a chemical compound with the general formula (1):Ar--?--CR.sub.1 R.sub.2 --!n--?--C R.sub.3 R.sub.4 --!m--C R.sub.5 R.sub.6 --OH (1)in which Ar is phenyl, alkyl-substituted phenyl, naphthyl, or alkyl-substituted naphthyl, R.sub.1 to R.sub.6 is H, OH or alkyl and n and m is 0 or 1;ii) incubating; andiii) isolating the crystals is provided. The crystals are in the form of needles, trigonal forms, cubes or parallelepipeds with a length of at least 20 microns.Type: GrantFiled: May 24, 1995Date of Patent: March 31, 1998Assignee: Pharmacia & Upjohn AktiebolagInventors: Ebba Florin-Robertsson, Elvy Hokby, Ronny Lundin, Sirkka Thome, Gertrud Westin-Sjodahl
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Patent number: 5663304Abstract: A composition is provided comprising about 0.1 to 15 mg/mL of a polypeptide in a buffer having a pH of about 7-12 comprising about 5-40% (v/v) of an alcoholic or polar aprotic solvent, about 0.2 to 3M of an alkaline earth, alkali metal, or ammonium salt, about 0.1 to 9M of a chaotropic agent, and about 0.01 to 15 .mu.M of a copper or manganese salt. The buffer is suitably used in a method for refolding improperly folded polypeptides.Type: GrantFiled: August 20, 1993Date of Patent: September 2, 1997Assignee: Genentech, Inc.Inventors: Stuart Builder, Roger Hart, Philip Lester, David Reifsnyder
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Patent number: 5605577Abstract: A process wherein a grain flour is treated to remove proteins is described. The process uses ethanol and water for the extraction at acid or basic pH's and optionally heating with or without a reducing agent. The remaining solution is then used to form edible and biodegradable films by casting on a surface.Type: GrantFiled: November 28, 1995Date of Patent: February 25, 1997Assignee: Board of Trustees operating Michigan State UniversityInventors: Luis M. Rayas, Perry K.W. Ng
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Patent number: 5532141Abstract: A method is taught whereby improved yields of lipid containing molecules, such as gangliosides, are obtained. Involved is the treatment of neural tissue from animals afflicted with GM1 gangliosidosis. Only members of the ovine family, e.g., sheep are so treated.Type: GrantFiled: June 13, 1995Date of Patent: July 2, 1996Inventor: Larry D. Holler
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Patent number: 5530100Abstract: Recovery of the 52/48 kDa tryptic fragment of vWF or peptide subfragments thereof produced in the form of inclusion bodies from recombinant host cells is carried out by providing a washed recombinant host cell suspension, adding a detergent and subjecting the cells to mechanical disruption. A second detergent is added, followed by another mechanical disruption and centrifugation to a pellet. The pellet is resuspended in a buffer and subjected to another mechanical disruption. Inclusion bodies are washed, resuspended and then recovered. In addition, endotoxins and DNA are removed from inclusion bodies containing the 52/48 kDa tryptic fragment of vWF or peptide subfragments thereof by mechanically disrupting the inclusion bodies in an aqueous buffer containing a detergent. A washed pellet is formed from these mechanically disrupted inclusion bodies and dissolved in a denaturant.Type: GrantFiled: May 7, 1990Date of Patent: June 25, 1996Assignee: Rhone-Poulenc Rorer Pharmaceuticals Inc.Inventors: Thomas L. J. Darling, Lida Y. Akhnana, Jonathan J. Mitschelen, Michael E. Hrinda
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Patent number: 5472511Abstract: A process wherein a grain flour is treated to remove proteins is described. The process uses ethanol and water for the extraction at acid or basic pH's and optionally heating with or without a reducing agent. The remaining solution is then used to form edible and biodegradable films by casting on a surface.Type: GrantFiled: July 11, 1994Date of Patent: December 5, 1995Assignee: Board of Trustees operating Michigan State UniversityInventors: Luis M. Rayas, Perry K. W. Ng
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Patent number: 5275954Abstract: A process and apparatus are disclosed for the demineralization of ground bone particles or pieces of cancellous or cortical bone. The apparatus includes multiple solution reservoirs which supply solutions to be pumped into one or more columns filled with bone samples to be demineralized. Solvent outflowing from the column(s) can be monitored for pH, calcium ion concentration or conductivity as a basis for determining when demineralization is complete. During the delipidization phase of processing, lipid solute can be monitored by photometry, or a small assay of the eluent can be dropped into deionized water to see whether a precipitate develops. Flow through the apparatus, including the types and amounts of solutions to be flowed through the apparatus, can be manually or computer controlled.Type: GrantFiled: March 5, 1991Date of Patent: January 4, 1994Assignee: LifenetInventors: Lloyd Wolfinbarger, Scott Bottenfield
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Patent number: 5258496Abstract: Methods are provided for the isolation and purification of recombinant lung surfactant protein. The methods involve a multistep procedure including extraction with a C.sub.1 -C.sub.4 aliphatic alcohol and chromatographic purification steps which employ a C.sub.1 -C.sub.4 aliphatic alcohol as eluant. Purified proteins obtained using the disclosed isolation and purification steps are provided as well.Type: GrantFiled: July 10, 1990Date of Patent: November 2, 1993Assignee: Scios Nova Inc.Inventors: Bradley J. Benson, Douglas Buckley, David Lesikar, Asha Naidu, Kate B. Silverness
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Patent number: 5254673Abstract: This invention pertains to methods for extracting and purifying zein, zein bodies, glutelins or destarched corn gluten from corn gluten meal by efficiently removing and recovering food grade pigments and starch hydrolysates, while discarding unwanted fractions. Corn gluten is subjected to the combination of enzymatic starch hydrolysis, alkaline treatment, alcohol washing and alcohol extraction to produce a substantially starch-free, deflavored and decolored zein which is suitable for use in foods and pharmaceuticals. An advantage of the process described herein is its use of food grade alcohol(s) to deflavor, decolorize and extract the zein from corn gluten meal. Purified zein and zein bodies have improved utility and functionality in a variety of existing and emerging food applications. Alternate products such as deflavored, decolored and destarched corn gluten, zein bodies or glutelins are also obtained by the methods of this invention and are useful as vegetable protein supplements.Type: GrantFiled: December 26, 1991Date of Patent: October 19, 1993Assignee: Opta Food Ingredients, Inc.Inventors: Richard B. Cook, Frank M. Mallee, Mark L. Shulman
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Patent number: 5229497Abstract: Casings for food such as sausage are prepared from connective tissue that has been removed from animal tissue in accordance with the process comprising the steps of (1) mechanically separating connective tissue and impurities from animal tissue; (2) treating the connective tissue to remove the fat; (3) reducing the residual bone content of the connective tissue by exposure to acidic materials; (4) separation of the non-collagenous protein and elastin by treating the connective tissue with a first enzyme; and (5) separation of the muscle tissue from the connective tissue by treating with a second enzyme. The step of removing the fat from the connective tissue can be accomplished by one or more of the following steps: (A) hydrocarbon extraction of the fat from the connective tissue; (B) extraction of the connective tissue with a critical fluid; (C) dissolution of the fat with a third enzyme; (D) treatment of the fat with a suitable treating agent; (E) heat treatment; and/or (F) pressure treatment e.g.Type: GrantFiled: October 3, 1990Date of Patent: July 20, 1993Assignee: Teepak, Inc.Inventor: Kenneth A. Boni
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Patent number: 5215963Abstract: A procedure for the preparation of the solubilized and purified gastrin releasing peptide receptor, in an active form, from a gastrin releasing peptide receptor source such as Swiss 3T3 fibroblasts.Type: GrantFiled: October 24, 1989Date of Patent: June 1, 1993Assignee: Berlex Laboratories, Inc.Inventors: Richard I. Feldman, James M. Wu, Elaina Mann, Anne Larocca, James C. Jenson
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Patent number: 5210184Abstract: A process for enhancing the functional properties of denatured proteinaceous material of vegetable origin. The enhanced properties include at least one property selected from the group consisting of water absorption, water binding capacity, oil binding capacity, fat binding capacity, and the ability to produce viscous aqueous suspensions. The process includes the steps of obtaining the denatured proteinaceous material by treating undenatured proteinaceous material with aqueous alcohol and maintaining a slurry of the denatured proteinaceous material in warm aqueous ammonia in which the weight ratio of the aqueous phase to solids is between 3:1 and 15:1 at a temperature between 75.degree. C. to 100.degree. C. and within a pH range of from 8.0 to 9.5.Type: GrantFiled: June 18, 1991Date of Patent: May 11, 1993Inventor: Daniel Chajuss
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Patent number: 5155040Abstract: Alcohol is suitable for the extraction of salt from biomass-containing suspensions, in particular from the salt-containing lower phase of an aqueous 2-phase extraction of intracellular proteins after cell disruption, with the formation of a salt-containing alcoholic upper phase. It is possible to use ethanol, propanol, isopropanol or tert.-butanol, but especially ethanol, as the alcohol. The upper phase is separated from the lower phase by disk separators or decanters. The extraction is carried out, in particular, as a countercurrent extraction in at least three stages, and uses 10 to 30% by weight salt-containing suspension or liquid with 30 to 50% by weight alcohol, in particular ethanol, remainder water. The alcohol is removed from the resulting salt-rich upper phase by evaporation, and the salt solution is recycled where appropriate after further concentration to obtain proteins.Type: GrantFiled: March 15, 1990Date of Patent: October 13, 1992Assignee: Forschungszentrum Juelich GmbHInventors: Maria-Regina Kula, Arend Greve
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Patent number: 5124256Abstract: The invention relates to a process for releasing into aqueous medium polypeptides produced by yeasts and localized at least partially within the periplasmic space thereof without breaking the yeast cell. The process involves treating the yeasts in aqueous medium with a neutral water-soluble mineral salt and a non-ionic water-soluble polyethoxylated alkylphenol surfactant having an HLB of between 8 and 15.Type: GrantFiled: November 5, 1990Date of Patent: June 23, 1992Assignee: Labofina, S.A.Inventors: Jacques Crahay, Jean M. A. G. Delcour, Jacques D. V. Hanotier
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Patent number: 5112956Abstract: The present invention relates to a method for the removal of lipids and cholesterol from protein materials comprising the steps of (a) treating the protein with an extraction mixture comprising a lower alcohol, water and an acid, in concentrations selected to extract cholesterol and lipids from the protein, and (b) removing the extraction mixture from the protein.Type: GrantFiled: March 17, 1989Date of Patent: May 12, 1992Assignee: The NutraSweet CompanyInventors: Pamela S. Tang, Norman S. Singer, Hsien-Hsin Chang
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Patent number: 5097017Abstract: Process for making soy protein concentrate which includes agglomerating either dusty defatted soybean flakes or the dust itself in a screw device with substantially no die restriction at the discharge end, the material to be agglomerated having been subjected to moisture addition to bring the moisture content to 18-30%, maintaining the temperature in the range of 160.degree.-300.degree. and thereafter extracting the agglomerated material with 55-75% aqueous ethanol.Type: GrantFiled: December 13, 1990Date of Patent: March 17, 1992Assignee: Central Soya Company, Inc.Inventor: Arthur H. Konwinski
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Use of intermediate length alcohols as virucidal agents in solutions of biologically active proteins
Patent number: 5071650Abstract: Soluble, intermediate length alcohols (C.sub.4 -C.sub.10) in aqueous solutions at low pH (4.0-7.0) can be used as virucidal agents for therapeutic biologically active protein preparations. Treatment with the alcohols is especially useful for proteins having activity not adversely affected by low pH.Type: GrantFiled: June 4, 1990Date of Patent: December 10, 1991Assignee: Miles Inc.Inventors: George B. Dove, Milton B. Dobkin, Michael A. Shearer -
Patent number: 5047510Abstract: The present invention relates to improved methods for purifying TGF-beta so as to obtain such in high yields. The process steps consist of acid-ethanol extracting platelets, cation exchange separation, and hydrophobic separation.Type: GrantFiled: October 11, 1989Date of Patent: September 10, 1991Assignee: Ostuka Pharmaceutical Co. Ltd.Inventors: James L. Cone, Joseph E. DeLarco
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Patent number: 5017556Abstract: A method and therapeutic composition for the treatment of bleeding disorders, for example those characterized by a tendency toward hemorrhage or a hypercoagulative state, by the administration of tissue factor protein or antagonists thereof.Type: GrantFiled: March 8, 1989Date of Patent: May 21, 1991Assignee: Genentech, Inc.Inventors: Donogh P. O'Brien, Gordon A. Vehar
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Patent number: 4958007Abstract: A method is provided for extracting human interleukin-4 (IL-4) from IL-4 expressing bacterial cells. The method comprises treating the bacterial cells with a deactivating agent, disrupting the cell membrane and recovering the IL-4.Type: GrantFiled: May 17, 1988Date of Patent: September 18, 1990Assignee: Schering-Plough Corp.Inventors: Yair Alroy, Paul Leibowitz
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Patent number: 4931543Abstract: A process for recovering substantially pure rIL-2 from transformed microorganisms in which the cells are disrupted, impure insoluble rIL-2 is separated from the bulk of the cellular components, the separated impure rIL-2 is solubilized and partially purified in a reduced form, the solubilized rIL-2 is oxidized, the oxidized rIL-2 is purified to clinically acceptable levels, and the oxidized, purified IL-2 is denatured by placing it into a solution of a chaotropic agent, solids are removed from the solution and rIL-2 is renatured from the solution.Type: GrantFiled: March 25, 1988Date of Patent: June 5, 1990Assignee: Cetus CorporationInventors: Robert Halenbeck, Flint Smith, Michael Kunitani
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Patent number: 4902673Abstract: A bidifobacteria growth promoter contained in soybeans is extracted from defatted soybeans with an aqueous solution of alcohol.Type: GrantFiled: November 9, 1988Date of Patent: February 20, 1990Assignee: The Calpis Food Industry Co., Ltd.Inventors: Kunihiko Hayakawa, Teruhisa Masai, Yasuyuki Yoshida, Takanobu Shibuta, Hiroshi Miyazaki
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Patent number: 4883865Abstract: A liquid phase containing preS2+S antigen is separated into two phases after which the desired antigen is concentrated, washed and adsorbed and desorbed from a fumed silica to produce a product that is purified and concentrated in antigen:protein ratio and that is suitable for final purification.Type: GrantFiled: September 30, 1987Date of Patent: November 28, 1989Assignee: Merck & Co. Inc.Inventor: Dennis J. Kubek
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Patent number: 4873317Abstract: A method for the separation or purification of biopolymers comprises adsorbing the biopolymer on the surface of liquid oil droplets; and separating the adsorbed biopolymer from the oil droplets. The adsorbed biopolymer is separated from the oil droplets by mixing the droplets in an aqueous liquid, removing the lower aqueous phase and adding a fresh aqueous phase to the droplets, cooling the mixture to solidify and coalesce the oil and to cause it to release the adsorbed biopolymer to the fresh liquid, separating the fresh liquid and the biopolymer from the coalesced oil, separating the biopolymer from the fresh liquid.Type: GrantFiled: March 5, 1987Date of Patent: October 10, 1989Inventors: Melvyn Rosenberg, Ilana Eli
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Patent number: 4870053Abstract: A novel process for the preparation of water-soluble acyl glycoprotein extracted from Klebsiella Pneumoniae containing 30 to 45% by weight of proteins, 30 to 40% by weight of neutral saccharides, less than 4% of glucuronic acid, 2 to 5% by weight of osamines with a molecular weight of about 350,000 daltons and having a polysaccharide chain of n chains of one molecule of glucose and 4 molecules of galactose attached to an asparagine of a proteidic chain by a core formed of heptose and 2-keto-3-deoxy-octulosonic acid followed by an acyl portion containing .beta.-hydroxymyristic acid and then N-acetyl-glucosamine designated herein as F.sub.1 and compositions and a method of inducing antiallergic properties in warm-blooded animals.Type: GrantFiled: January 4, 1984Date of Patent: September 26, 1989Assignee: Roussel UclafInventors: Rene Zalisz, Marie-France Salles
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Patent number: 4757134Abstract: Describes IgA binding protein isolatable from Group B streptococci, methods of isolation and use in immunological testing procedures.Type: GrantFiled: February 13, 1986Date of Patent: July 12, 1988Assignee: The Rockefeller UniversityInventors: Milan Blake, Emil Gotschlich, Gregory J. Russell-Jones
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Patent number: 4748234Abstract: A refractile material containing a heterologous protein is recovered from a host microorganism cell culture transformed to produce the protein. One recovery process involves disrupting the cell wall and membrane of the host cell, removing greater than 99% by weight of the salts from the disruptate, redisrupting the desalted disruptate, adding a material to the disruptate to create a density or viscosity gradient in the liquid within the disruptate, and separating the refractile material from the cellular debris by high-speed centrifugation. Another version of such a recovery process comprises the further steps of solubilizing the refractile material under reducing conditions, organically extracting the solubilized refractile material, and isolating said refractile material from the extractant.Preferably the protein is recombinant IL-2 or IFN-.beta. and the salt removal step is carried out by diafiltration.Type: GrantFiled: March 25, 1986Date of Patent: May 31, 1988Assignee: Cetus CorporationInventors: Glenn Dorin, Wolfgang H. Hanisch, Leo S. Lin
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Patent number: 4697003Abstract: There is disclosed a method for separating alpha-1-proteinase inhibitor (also known as alpha-1 antitrypsin) from an aqueous solution of plasma proteins, especially from Cohn Effluent II & III and Cohn Effluent I. The method includes the steps of first treating the aqueous solution to lower the concentration of salts therein and, optionally, its alcohol content, contacting the resulting solution with an anion exchange resin having selective affinity for alpha-1-proteinase inhibitor to selectively bind the alpha-1-proteinase inhibitor and allow unwanted plasma proteins to elute through the resin, displacing the alpha-1-proteinase inhibitor from the resin and recovering the same.Type: GrantFiled: November 1, 1985Date of Patent: September 29, 1987Assignee: Miles Laboratories, Inc.Inventor: Michael H. Coan
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Patent number: 4690892Abstract: A method is disclosed for the recovery of biological material from an aqueous solution comprising contacting a water-insoluble, particulate binder with a solution containing biological material to produce a water insoluble biological material/binder composition which may then be recovered. The aqueous solutions may then be recycled.Type: GrantFiled: February 12, 1986Date of Patent: September 1, 1987Assignee: Union Carbide CorporationInventors: Kavssery P. Ananthapadmanabhan, Errol D. Goodard
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Patent number: 4683294Abstract: The process is applicable to the supernatant of engineered yeast cells disrupted in the presence of a non-ionic detergent: it comprises the precipitation of contaminants by polyethylene glycol and the treatment cation or, after eventual ultrafiltration, with ammonium sulfate.Type: GrantFiled: April 3, 1985Date of Patent: July 28, 1987Assignee: Smith Kline RIT, S.A.Inventors: Frans Van Wijnendaele, Daniel Gilles, Guy Simonet
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Patent number: 4668584Abstract: A method is provided for forming ordered macromolecular protein arrays by avoiding a binding pathway that leads to densely packed, disordered states during protein crystal growth, by a diffusion limited process or by allowing controlled growth to occur by removal of the initial supported protein layer to a different environment.Type: GrantFiled: December 23, 1985Date of Patent: May 26, 1987Assignee: General Electric CompanyInventors: Egidijus E. Uzgiris, James R. Yates
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Patent number: 4665161Abstract: Crude HCG is purified by extracting with a neutral or weakly basic aqueous solution containing lower aliphatic alcohol and soluble salt, adding lower aliphatic alcohol to the extracted solution to form precipitates and the precipitates containing high purity of HCG are collected. This precipitates can be further purified by dissolving in a buffer solution, contacting the solution with a weak anion exchanger and eluting the exchanger with said buffer solution containing added salt.Type: GrantFiled: April 21, 1986Date of Patent: May 12, 1987Assignee: Japan Chemical Research Co., Ltd.Inventors: Yoshikazu Yuki, Toyohiko Nishimura, Hajime Hiratani
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Patent number: RE36259Abstract: A composition is disclosed which comprises a solution of human serum albumin essentially free of chemicals used in processing. The preparation is also essentially free of metals such as aluminum. The composition is 100% pure by cellulose acetate electrophoresis and is essentially monomeric when tested by high pressure liquid chromatography. The turbidity is less than 5 N.T.U. (National Turbidity Units). This preparation has a substantially longer shelf life and remains biologically active longer than products currently available. The novelty of this product is also such that it does not leach metallic substances such as aluminum from its closure. Novel applications of process methodology are taught in the preparation of this composition and a novel preparation results from essentially non hemoglobin containing albumin sources such as Source Plasma (Human).Type: GrantFiled: July 18, 1994Date of Patent: July 27, 1999Assignee: Bayer CorporationInventor: Robert A. Tenold