Encodes An Enzyme Patents (Class 536/23.2)
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Patent number: 8951766Abstract: Provided herein are isolated laccase enzymes and nucleic acids encoding them. Also provided are mediators for laccase reactions. Also provided herein are methods for using laccases to oxidize lignins and other phenolic and aromatic compounds, such as for bio-bleaching and decolorization of wood pulp under high temperature and pH conditions to facilitate a substantial reduction in use of bleaching chemicals, as well as for treatment of fibers.Type: GrantFiled: January 18, 2013Date of Patent: February 10, 2015Assignee: BASF Enzymes LLCInventors: Janne Samuli Kerovuo, Sylke Haremza, Oliver Koch, Tilo Habicher, Dan E. Robertson, Grace Desantis, Ryan McCann, Peter Luginbuhl
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Patent number: 8951774Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.Type: GrantFiled: November 4, 2010Date of Patent: February 10, 2015Assignee: Butamax Advanced Biofuels LLCInventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
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Publication number: 20150037868Abstract: Various aspects and embodiments herein relate to recombinant proteins with at least one protease recognition sequence that can be inactivated by a cognate protease and methods of preparing such proteins. In some embodiments, recombinant phosphoglucose isomerase (Pgi) proteins are provided. In other embodiments, recombinant phosphotransacetylase (Pta) proteins are provided. In yet other embodiments, recombinant transketolase A (TktA) proteins are provided.Type: ApplicationFiled: August 5, 2014Publication date: February 5, 2015Applicant: GreenLight Biosciences, Inc.Inventors: William Jeremy Blake, Drew S. Cunningham
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Publication number: 20150037829Abstract: An imaging method comprising expressing in cells a Class I heme peroxidase, which optionally is fused with a protein of interest or a cellular localization signal peptide, and contacting the cells with a substrate of the Class I heme peroxidase to allow conversion of the substrate into a product via an oxidation reaction catalyzed by the Class I heme peroxidase, wherein the product releases a signal detectable by a microscope such as an electron microscope. Also disclosed herein are monomeric mutants of a Class I heme peroxidase and mutants of the enzyme that exhibit elevated enzymatic activity as compared to the corresponding wild-type counterpart.Type: ApplicationFiled: September 14, 2012Publication date: February 5, 2015Applicant: Massachusetts Institute of TechnologyInventors: Alice Y. Ting, Jeffrey Daniel Martell
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Publication number: 20150037312Abstract: The present invention provides polypeptides that bind to immunoglobulin G and methods for their use.Type: ApplicationFiled: March 25, 2013Publication date: February 5, 2015Inventors: David Baker, Sarel Jacob Fleishman, Eva-María Strauch
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Publication number: 20150037869Abstract: The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: ApplicationFiled: October 20, 2014Publication date: February 5, 2015Inventors: Christopher K. Savile, Emily Mundorff, Jeffrey C. Moore, Paul N. Devine, Jacob M. Janey
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Publication number: 20150037854Abstract: Provided herein are diterpene synthases (diTPS) and methods for producing diterpenoids. Also provided herein are nucleic acid sequences encoding diTPS, diTPS amino acid sequences, diTPS proteins, vectors, cells, transgenic organisms, uses, compositions, methods, processes, and kits thereof.Type: ApplicationFiled: September 18, 2014Publication date: February 5, 2015Inventors: Joerg Bohlmann, Philipp Zerbe
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Publication number: 20150037845Abstract: The present disclosure relates to mutant thermostable glycosyl hydrolases family 7 enzymes, including mutant Trichoderma reesei endoglucanase I. In particular, the present disclosure relates to mutant thermostable enzymes, compositions containing the enzymes, and methods of use thereof.Type: ApplicationFiled: August 11, 2011Publication date: February 5, 2015Applicant: The Regents of the University of CaliforniaInventors: Harshal Akshay Chokhawala, Tae-Wan Kim, Harvey W. Blanch, Douglas S. Clark
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Publication number: 20150037871Abstract: Hybrid nuclease molecules and methods for treating an immune-related disease or disorder in a mammal, and a pharmaceutical composition for treating an immune-related disease in a mammal.Type: ApplicationFiled: October 16, 2014Publication date: February 5, 2015Inventors: Jeffrey A. Ledbetter, Martha Hayden-Ledbetter, Keith Elkon, Xizhang Sun
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Publication number: 20150037284Abstract: The present invention relates to a novel Listeria bacteriophage designated ProCC P825. In particular, the present invention relates to the endolysin PlyP825 encoded by the novel phage ProCC P825 and uses of the novel endolysin PlyP825 for controlling Listeria contamination and infection.Type: ApplicationFiled: May 29, 2012Publication date: February 5, 2015Applicant: DSM IP ASSETS B.V.Inventors: Holger Grallert, Julia Lorenz, Anna Scherzinger
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Publication number: 20150038331Abstract: Rice is described that is tolerant/resistant to a plurality of herbicides, for example, ACCase and HPPD inhibitors. Use of the rice for weed control and methods of producing tolerant/resistant rice are also described.Type: ApplicationFiled: August 21, 2014Publication date: February 5, 2015Inventors: Melissa Hinga, Melissa Shannon Moon, Venu Reddyvari CHANNARAYAPPA, Russell D. RASMUSSEN, Federico CUEVAS
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Patent number: 8945859Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.Type: GrantFiled: March 15, 2013Date of Patent: February 3, 2015Assignee: Butamax Advanced Biofuels LLCInventors: Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
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Patent number: 8945864Abstract: The object of the present invention is to provide a method of determining 1,5-anhydroglucitol, including using (a) a protein which consists of the amino acid sequence of SEQ ID NO: 2; (b) a protein which consists of an amino acid sequence having deletion, substitution and/or addition of one or more amino acid residues in the amino acid sequence of SEQ ID NO: 2 and which has sorbose dehydrogenase activity; or (c) a protein which consists of an amino acid sequence having a homology of at least 60% with the amino acid sequence of SEQ ID NO: 2 and which has sorbose dehydrogenase activity.Type: GrantFiled: June 22, 2007Date of Patent: February 3, 2015Assignees: Ikeda Food Research Co., Ltd., Nippon Kayaku Kabushiki KaishaInventors: Emi Ishimaru, Hirokazu Sanada, Hironori Omura, Hideki Yoshioka, Shuhei Tsukamoto, Minoru Masuda
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Patent number: 8945888Abstract: The present invention relates to an improved method for the bioconversion of a fermentable carbon source to glycolic acid by a recombinant microorganism bearing new genetic modifications such as ?ldhA, ?mgsA, ?arcA, and ?lldP, ?glcA, ?yjcG and combination of them allowing a production with higher yield, titer and productivity.Type: GrantFiled: March 23, 2010Date of Patent: February 3, 2015Assignee: Metabolic ExplorerInventors: Wanda Dischert, Philippe Soucaille
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Patent number: 8945899Abstract: Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.Type: GrantFiled: December 15, 2009Date of Patent: February 3, 2015Assignee: Butamax Advanced Biofuels LLCInventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
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Patent number: 8946507Abstract: A method of producing male or female sterile plants comprising the steps of transforming plant material with a polynucleotide which encodes at least one enzyme which reacts with a non-phytotoxic substance to produce a phytotoxic one, and regenerating the thus transformed material into a plant, wherein the said non-phytotoxic substance is applied to the plant up to the time of male or female gamete formation and/or maturation, so that the non-phytotoxic substance provides for the production of a phytotoxic one which selectively prevents the formation of or otherwise renders the said gametes non-functional, wherein the enzyme is expressed preferentially in either male or female reproductive structures, characterised in that (i) the non-phytotoxic substance is D-phosphinothricin and (ii) the enzyme is a D-amino acid oxidase.Type: GrantFiled: June 21, 2010Date of Patent: February 3, 2015Assignee: Syngenta LimitedInventors: Timothy Robert Hawkes, Glynn Mitchell, Stephen Thomas Hadfield, Paul Anthony Thompson, Russell Viner, Yan Zhang
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Patent number: 8945875Abstract: Disclosed are nucleic acid and amino acid sequences for acetolactate synthase, acetolactate synthase regulatory regions, ?-tubulin promoter, a promoter from a Thraustochytriales polyketide synthase (PKS) system, and fatty acid desaturase promoter, each from a Thraustochytriales microorganism. Also disclosed are recombinant vectors useful for transformation of Thraustochytriales microorganisms, as well as a method of transformation of Thraustochytriales microorganisms. The recombinant nucleic acid molecules of the present invention can be used for the expression of foreign nucleic acids in a Thraustochytriales microorganism as well as for the deletion, mutation, or inactivation of genes in Thraustochytriales microorganisms.Type: GrantFiled: June 21, 2013Date of Patent: February 3, 2015Assignee: DSM IP Assets B.V.Inventors: Paul G. Roessler, T. Dave Matthews, Tom M. Ramseier, James G. Metz
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Patent number: 8945903Abstract: The present invention relates to cellobiohydrolase variants having improved thermostability in comparison to wild-type CBH2a.Type: GrantFiled: July 13, 2012Date of Patent: February 3, 2015Assignee: Codexis, Inc.Inventors: Dipnath Baidyaroy, Louis Clark, David Elgart, Rama Voladri, Xiyun Zhang
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Patent number: 8945901Abstract: Butyrylcholinesterase (BChE) polypeptide variants of the presently-disclosed subject matter have enhanced catalytic efficiency for (?)-cocaine, as compared to wild-type BChE. Pharmaceutical compositions of the presently-disclosed subject matter include a BChE polypeptide variant having an enhanced catalytic efficiency for (?)-cocaine. A method of the presently-disclosed subject matter for treating a cocaine-induced condition includes administering to an individual an effective amount of a BChE polypeptide variant, as disclosed herein, to lower blood cocaine concentration.Type: GrantFiled: October 23, 2013Date of Patent: February 3, 2015Assignee: University of Kentucky Research FoundationInventors: Chang-Guo Zhan, Fang Zheng, Wenchao Yang, Liu Xue, Shurong Hou
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Publication number: 20150031098Abstract: Provided is a mutant of propionyl-CoA transferase from Clostridium propionicum that can convert lactate into lactyl-CoA with high efficiency in a method of preparing a polylactate (PLA) or PLA copolymer using microorganisms. Unlike conventional propionyl-CoA transferase which is weakly expressed in E. coli, when a mutant of propiony-CoA transferase from Clostridium propionicum is introduced into recombinant E. coli, lactyl-CoA can be supplied very smoothly, thereby enabling highly efficient preparation of polylactate (PLA) and PLA copolymer.Type: ApplicationFiled: October 7, 2014Publication date: January 29, 2015Inventors: Si Jae PARK, Taek Ho YANG, Hye Ok KANG, Sang Hyun LEE, Eun Jung LEE, Tae Wan KIM
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Publication number: 20150031020Abstract: The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.Type: ApplicationFiled: August 8, 2014Publication date: January 29, 2015Inventors: Lakmal JAYASINGHE, John Hagan Pryce BAYLEY, Stephen CHELEY, Brian MCKEOWN, James WHITE, James Anthony CLARKE
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Publication number: 20150031091Abstract: The present invention provides isolated polypeptides having alpha-amylase activity catalytic domains, carbohydrate binding domains and polynucleotide encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains.Type: ApplicationFiled: September 29, 2012Publication date: January 29, 2015Applicant: NOVOZYMES A/SInventors: Ming Li, Junxin Duan, Noriko Tsutsumi, Guillermo Coward-Kelly, Henrik Lundkvist
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Publication number: 20150031093Abstract: To produce quinolinate effectively, a L-aspartate oxidase variant that the feedback regulation by nicotinic acid or NAD is released, and a microorganism including the L-aspartate oxidase variant are provided. Quinolinate may be effectively produced by culturing of the microorganism including the L-aspartate oxidase variant.Type: ApplicationFiled: July 25, 2014Publication date: January 29, 2015Inventors: So Young KIM, Yong Uk SHIN, In Kyung HEO, Ju Eun KIM, Kwang Ho NA, Chang Il SEO, Sung Kwang SON, Jae Hee LEE
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Publication number: 20150031059Abstract: An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. The flavin-binding glucose dehydrogenase of the invention has the following characteristics (1) and (4): (1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 68 kDa as measured by SDS-polyacrylamide electrophoresis; (2) Km value: the Km value for D-glucose is about 15 mM or less; (3) Temperature stability: stable at a temperature of 55° C. or less; and (4) pH stability: stable at a pH range of 3.0 to 8.5.Type: ApplicationFiled: February 7, 2013Publication date: January 29, 2015Applicant: TOYOBO CO., LTD.Inventors: Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroshi Kawaminami, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
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Publication number: 20150031109Abstract: The present invention provides compositions and methods for producing a polyol oxidase in micoroorganisms, and the use of polyol oxidases in cleaning compositions. The invention includes cleaning compositions that contain combinations of two or more POx oxidases, and cleaning compositions that contain combinations of two or more POx oxidases and a perhydrolase. In particular, the invention provides methods for expressing polyol oxidases in bacterial hosts for use in detergent applications for cleaning, bleaching and disinfecting.Type: ApplicationFiled: July 30, 2014Publication date: January 29, 2015Applicant: DANISCO US INC.Inventors: Manoj KUMAR, Susan M. MADRID, Hugh C. MCDONALD, Ayrookaran J. POULOSE, Thomas RAND, Huaming WANG
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Publication number: 20150031094Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.Type: ApplicationFiled: October 1, 2014Publication date: January 29, 2015Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt W. Huisman, Steven J. Collier
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Publication number: 20150030601Abstract: The invention provides isolated nucleic acids molecules, designated UBA3, UAE, or UBA6, or other E1 enzyme variant nucleic acid molecules, which encode novel E1 enzyme variant proteins. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing UBA3, UAE, or UBA6, or other E1 enzyme variant nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a UBA3, UAE, or UBA6, or other E1 enzyme variant gene has been introduced or disrupted. The invention still further provides isolated UBA3, UAE, or UBA6, or other E1 enzyme variant proteins, fusion proteins, antigenic peptides and anti-UBA3, UAE, or UBA6, or other E1 enzyme variant antibodies. The invention provides methods to identify agents that inhibit UBA3, UAE, or UBA6, or other E1 enzyme variant expression or activity. Diagnostic and therapeutic methods utilizing compositions of the invention are also provided.Type: ApplicationFiled: October 5, 2012Publication date: January 29, 2015Applicant: Millennium Pharmaceuticals, Inc.Inventors: Benjamin Stone Amidon, James E. Brownell, James M. Gavin, Erik M. Koenig, Michael D. Sintchak, Peter G. Smith
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Publication number: 20150030717Abstract: The present teachings provide modified enzymes, preferably phytases, which have increased stability, hypothesized to arise from increased glycosylation. The enzymes can be modified to introduce or increase the number of glycosylation sites in the amino acid sequence, or glycosylation can be increased by the use of specific host production methods, or both. The enzymes of the present teachings have an increased stability after treatment at elevated temperature, which can be measured by inactivity reversibility or percent recovery following a treatment such as heating. The enzymes of the present teachings find application for example in food, feed, and feed pelleting.Type: ApplicationFiled: February 1, 2013Publication date: January 29, 2015Inventors: Mark S. Gebert, Sang-Kyu Lee, Mariliz Ortiz-Maldonado, Michael Ward
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Publication number: 20150031076Abstract: The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolized by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.Type: ApplicationFiled: October 2, 2014Publication date: January 29, 2015Inventors: Hubertus Johannes Marie OP DEN CAMP, Harry Ramanoedj HARHANGI, Christiaan VAN DER DRIFT, Jacobus Thomas PRONK
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Publication number: 20150031080Abstract: The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: October 13, 2014Publication date: January 29, 2015Inventor: Mark Wogulis
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Publication number: 20150030581Abstract: The invention provides for the use of a therapeutic derived from a truncated lipoprotein lipase protein (LPL S447X), including nucleic acids encoding such proteins, for the treatment of conditions including LPL responsive conditions, such as cardiovascular disease, hypertension, LPL deficiency, high triglyceride levels, low HDL-cholesterol levels or atherosclerosis.Type: ApplicationFiled: July 4, 2014Publication date: January 29, 2015Inventors: Michael R. HAYDEN, John P. Kastelein
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Publication number: 20150031096Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding multizymes (i.e., single polypeptides having at least two independent and separable enzymatic activities) along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these multizymes in plants and oleaginous yeast are disclosed.Type: ApplicationFiled: July 22, 2014Publication date: January 29, 2015Inventors: HOWARD GLENN DAMUDE, Anthony J. Kinney, Kevin G. Ripp, Quinn Qun Zhu
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Publication number: 20150031111Abstract: The present invention relates to isolated lipase variants, comprising a substitution at one or more positions corresponding to positions I86D,E,N,Q, E87C, I90D,E,Q, and N92D,E,Q of the mature polypeptide of SEQ ID NO: 2, wherein the variant has lipase activity. In some embodiments the present invention relates to isolated polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; methods of producing the variants; and compositions comprising the variants. The present invention also relates to methods of obtaining lipase variants; methods of cleaning; and use of lipase variants for cleaning.Type: ApplicationFiled: March 22, 2013Publication date: January 29, 2015Inventors: Jakob Skjold-Joergensen, Jesper Vind, Allan Svendsen
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Patent number: 8940515Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: November 8, 2010Date of Patent: January 27, 2015Assignee: Novozymes, Inc.Inventors: Alfredo Lopez de Leon, Michael Rey
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Patent number: 8940295Abstract: The present invention comprises methods and compositions for the reduction of oxalate in humans, and methods for the purification and isolation of recombinant oxalate reducing enzyme proteins. The invention provides methods and compositions for the delivery of oxalate-reducing enzymes in particle compositions. The compositions of the present invention are suitable in methods of treatment or prevention of oxalate-related conditions.Type: GrantFiled: March 27, 2013Date of Patent: January 27, 2015Assignee: Oxthera Intellectual Property ABInventors: Harmeet Sidhu, Aaron Blake Cowley, Carl-Gustaf Golander, Qingshan Li
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Patent number: 8940510Abstract: The invention provides spray-dried preparations of microbes and methods of using those microbes.Type: GrantFiled: November 16, 2007Date of Patent: January 27, 2015Assignee: University of Iowa Research FoundationInventors: Venkiteswaran Subramanian, James H. Glenn, IV, Shuvendu Das
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Patent number: 8940514Abstract: A thioesterase comprising an amino acid sequence set forth in SEQ ID NO: 1, a thioesterase gene encoding the thioesterase, a transformant comprising the gene, and a method of producing fatty acids or lipids using the transformant.Type: GrantFiled: April 13, 2011Date of Patent: January 27, 2015Assignee: Kao CorporationInventors: Takuto Tojo, Keiji Endo
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Publication number: 20150024485Abstract: This invention relates to industrial production of proteins. More specifically, the invention relates to the res-DHFR surrogate marker, which corresponds to a fusion between DHFR and a protein conferring resistance to a toxic compound or conferring a metabolic advantage. The invention further relates to the use of res-DHFR for screening cells for high expression of a protein of interest. The invention is illustrated by the Puro-DHFR surrogate marker, which corresponds to a fusion between the puromycin N-acetyltransferase and dihydrofolate reductase (DHFR).Type: ApplicationFiled: September 10, 2014Publication date: January 22, 2015Inventors: MICHEL KOBR, PHILIPPE DUPRAZ
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Publication number: 20150024461Abstract: An object is to provide a novel enzyme that exhibits glucose dehydrogenase activity. Furthermore, another object is to provide a novel method pertaining to enzyme modification. Provided is a mutated enzyme containing an amino acid sequence wherein one or at least two amino acids selected from a group are substituted with another amino acid in the amino acid sequence of a microorganism-derived glucose oxidase.Type: ApplicationFiled: October 7, 2014Publication date: January 22, 2015Inventors: Kyoichi Nishio, Satoshi Koikeda
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Publication number: 20150024439Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: September 29, 2014Publication date: January 22, 2015Inventors: Kimberly Brown, Paul Harris, Elizabeth Zaretsky, Edward Re, Elena Vlasenko, Keith McFarland, Alfredo Lopez de Leon
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Publication number: 20150026843Abstract: The present invention relates to isolated polypeptides having proteaseactivity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptidesin e.g. animal feed and detergents.Type: ApplicationFiled: December 20, 2012Publication date: January 22, 2015Inventors: Tine Hoff, Carsten Sjoeholm, Peter Rahbek Oestergaard, Katrine Pontoppidan
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Publication number: 20150024990Abstract: The present invention relates to novel subtilase variants exhibiting alterations relative to the parent subtilase in one or more properties including: Wash performance, thermal stability, storage stability or catalytic activity. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dishwash compositions, including automatic dishwash compositions.Type: ApplicationFiled: September 30, 2014Publication date: January 22, 2015Inventors: Tina Sejersgard Fano, Claus Von Der Osten, Malene Kappen Kruger, Mads Norregaard-Madsen
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Publication number: 20150024442Abstract: Provided are fusion proteins of diacylglycerol acyltransferase (DGAT) polypeptides fused to at least one heterologous intracellular localization domain, such as a bacterial membrane-targeting domain, modified photosynthetic microorganism that comprise such fusion proteins, and related methods for improving the production of lipids by photosynthetic microorganisms, including lipids such as triglycerides, fatty acids, and wax esters.Type: ApplicationFiled: March 4, 2013Publication date: January 22, 2015Applicant: Matrix Genetics, LLCInventors: James Roberts, Kimberly Marie Kotovic, Jason W. Hickman, Tracey Jurista, Michael Carleton
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Publication number: 20150024465Abstract: The present invention relates to non-catalytic carbohydrate-binding modules (CBM) belonging to a new family of CBM's. A CBM of the invention was found attached to a glycosyl hydrolase family 61 (GH61) polypeptide and was shown to have little homology with known CBM's indicating that it is the first known member of a new family of CBM's. The present invention further relates to CBM's preferably exhibiting binding affinity for cellulose; to a method of producing such CBM's; and to methods for using such CBM's in the textile, detergent and cellulose fiber processing industries, for purification of polypeptides, immobilisation of active enzymes, baking, manufacturing of biofuel, modification of plant cell walls.Type: ApplicationFiled: September 29, 2014Publication date: January 22, 2015Inventors: Kirk Matthew Schnorr, Lars Lehmann Hylling Christensen
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Publication number: 20150024464Abstract: Aspects of the invention provide engineered endonucleases that are characterized by both a long recognition sequence and specific cleavage outside of the recognition site. Engineered endonucleases of the invention are useful for manipulating long pieces of DNA.Type: ApplicationFiled: May 5, 2014Publication date: January 22, 2015Applicant: Celexion, LLCInventors: Shaun M. Lippow, Dasa Lipovsek, Patricia M. Aha
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Publication number: 20150023943Abstract: Purified Ref polypeptides with increased nuclease site-specific targeting activity, recombinant nucleic acids and cells for expression of such Ref polypeptides, and methods for using the Ref polypeptides in combination with RecA protein and variants thereof to effect targeted nuclease cleavage of a DNA duplex are disclosed.Type: ApplicationFiled: July 21, 2014Publication date: January 22, 2015Inventors: Michael Matthew Cox, Angela Jo Gruber, Tayla Maria Olsen
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Publication number: 20150023945Abstract: The present invention is directed to lysozyme variants and nucleotide sequences encoding same. The lysozyme variants have antimicrobial and/or lysozyme activity and comprise an alteration of an amino acid sequence at one or more positions. The present invention is also directed to methods for producing and of using the Opisthocomus hoazin lysozyme and the lysozyme variants of the present invention as antimicrobial agents.Type: ApplicationFiled: September 29, 2014Publication date: January 22, 2015Inventors: Mikkel Klausen, Marie Allesen Holm, Leonardo De Maria, Michael Skjoet
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Publication number: 20150024460Abstract: The present invention provides a novel fusion polypeptide containing a catalytic domain of NPP1 fused to a targeting moiety, nucleic acids encoding the fusion polypeptide, a vector containing the nucleic acid integrated thereinto, a host cell transformed with the vector and pharmaceutical compositions comprising the fusion polypeptide.Type: ApplicationFiled: October 6, 2014Publication date: January 22, 2015Inventors: Anthony Quinn, Alex J Harvey, Zhinan Xia
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Publication number: 20150024469Abstract: The present invention relates to a polypeptide with an amino acid sequence according to SEQ ID NO: 1 and fragments or derivatives thereof. The present invention further relates to fusion proteins comprising said polypeptide and an additional peptide stretch fused to said polypeptide at the N- or C-terminus. Moreover, the present invention relates to nucleic acid molecules encoding said polypeptide or fusion protein, vectors comprising said nucleic acid molecules and host cells comprising either said nucleic acid molecules or said vectors. In addition, the present invention relates to said polypeptide or fusion protein for use as a medicament, in particular for the treatment or prevention of Gram-negative bacterial infections, as diagnostic means, as cosmetic substance or as sanitizing agent.Type: ApplicationFiled: September 3, 2014Publication date: January 22, 2015Applicants: LYSANDO AG, KATHOLIEKE UNIVERSITEIT LEUVEN, K.U. LEUVEN R&DInventors: Yves BRIERS, Rob LAVIGNE, Maarten WALMAGH, Stefan MILLER
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Publication number: 20150024435Abstract: The present invention pertains to a mutated T7 RNA polymerase and its use, the T7 RNA polymerase being mutated at position 744, the glutamine (Q) being replaced by an amino acid selected from arginine (Q744R), leucine (Q744L) or proline (Q744P).Type: ApplicationFiled: October 8, 2012Publication date: January 22, 2015Inventors: Jean-Claude Boulain, Janie Dassa, Frédéric Ducancel, Bruno H. Muller, Alain Troesch, Laurent Mesta