Fungal Protein Patents (Class 536/23.74)
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Patent number: 7507574Abstract: The present invention provides a modified Saccharomyces yeast which produces significantly lower levels of ethanol than wild-type yeast under aerobic conditions and saccharide concentrations of 2% glucose, and which exhibits a growth rate of at least 30% of the wild-type yeast, preferably containing a chimeric construct of at least 2 saccharide transporters, nucleic acid molecules encoding the chimeras and polypeptides encoded by such sequences, and methods of using the modified yeast for preparing products in the yeast.Type: GrantFiled: June 29, 2001Date of Patent: March 24, 2009Assignee: Gothia Yeast Solutions ABInventors: Roslyn Bill, Eckhard Boles, Lena Gustafsson, Stefan Hohmann, Christer Larsson, Karin Elbing
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Publication number: 20090074913Abstract: The present invention relates to a gene encoding a protein responsible for flocculation property of yeast and use thereof, in particular, a brewery yeast with appropriate flocculation property for producing desired alcoholic beverages, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast to which suitable flocculation property for brewing desired alcoholic beverages was imparted by controlling expression level of MHP1 gene encoding a protein responsible for flocculation property of brewery yeast, especially non-ScMHP1 gene specific to a lager brewing yeast, to alcoholic beverages produced with said yeast and to a method for producing said beverages.Type: ApplicationFiled: December 27, 2006Publication date: March 19, 2009Applicant: SUNTORY LIMITEDInventors: Yoshihiro Nakao, Yukiko Kodama, Tomoko Shimonaga
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Patent number: 7504259Abstract: The present invention relates to fungal ?12 fatty acid desaturases that are able to catalyze the conversion of oleic acid to linoleic acid (LA; 18:2). Nucleic acid sequences encoding the desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the desaturase genes, and recombinant host microorganisms expressing increased levels of the desaturases are described. Methods of increasing production of specific ?-3 and ?-6 fatty acids by over-expression of the ?12 fatty acid desaturases are also described herein.Type: GrantFiled: November 10, 2004Date of Patent: March 17, 2009Assignee: E. I. du Pont de Nemours and CompanyInventors: Narendra S. Yadav, Quinn Qun Zhu, Hongxiang Zhang
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Publication number: 20090069256Abstract: Modified polynucleotide compositions providing enhanced gene expression and methods for preparing said compositions are disclosed. Methods of using the compositions, such as in screening assays, diagnostic tools, kits, etc. and for prevention and/or treatment of diseases and disorders are also disclosed.Type: ApplicationFiled: June 6, 2005Publication date: March 12, 2009Inventors: Larry R. Smith, Vafa Shahabi, Maninder K. Sidhu
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Publication number: 20090061427Abstract: The present invention relates to a gene encoding a protein responsible for flocculation property of yeast and use thereof in particular, a brewery yeast with appropriate flocculation property for producing desired alcoholic beverages, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast to which suitable flocculation property for brewing desired alcoholic beverages was imparted by controlling expression level of HSP150 gene encoding a protein responsible for flocculation property of brewery yeast, especially non-ScHSP150 gene specific to a lager brewing yeast, to alcoholic beverages produced with said yeast and to a method for producing said beverages.Type: ApplicationFiled: December 27, 2006Publication date: March 5, 2009Inventors: Yoshihiro Nakao, Yukiko Kodama, Tomoko Shimonaga
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Publication number: 20090053360Abstract: The present invention relates to a gene encoding glycogen synthase and use thereof, in particular, a yeast for practical use with superior resistance property to dryness and/or low-temperature storage, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose resistance property to dryness and/or resistance property to low-temperature storage is enhanced by amplifying expression level of GSY1 or GSY2 gene encoding a Gsy1p or Gsy2p which is a glycogen synthase in brewer's yeast, especially non-ScGSY1 gene or non-ScGSY2 gene specific to a lager brewing yeast and to a method for producing alcoholic beverages with said yeast, etc.Type: ApplicationFiled: February 1, 2007Publication date: February 26, 2009Applicant: Suntory LimitedInventors: Yoshihiro Nakao, Yukiko Kodama, Tomoko Shimonaga
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Publication number: 20090047701Abstract: The present invention relates to a fungus which produces substantially no functional protein with regulating activity on the transcription of genes which are involved in cell wall stress response. It also relates to a method for screening for antifungal agents, in particular to antifungal agents which target the cell wall. It also relates to a kit for screening for antifungal agents which disturb cell wall biogenesis.Type: ApplicationFiled: December 23, 2005Publication date: February 19, 2009Inventors: Arthur Franciscus Johannes Ram, Robbert Antonius Damveld, Mark Arentshorst, Patricia Ann van Kuyk, Cornelis Antonius Maria Jacobus Johannes van den Hondel
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Publication number: 20090042776Abstract: The invention relates to an improved nucleic acid molecule encoding fungal immunomodulatory protein (FIP) that is better expressed in fungi, to vectors comprising the nucleic acid molecule, to hosts transformed with said vectors, to processes of expressing the protein of the invention in said transformed hosts, to the protein of the invention produced by said processes, to uses of said hosts comprising the protein of the invention and to a process of purifying FIP. The protein of the invention has wide immunomodulatory activity. Thus, the present invention further relates to uses of the protein of the invention in cosmetic or pharmaceutical compositions and to food or feed additives comprising the protein of the invention. Finally, the invention relates to the method of modulating immunological activities by orally administering FIP or proteins fused with FIP to a subject.Type: ApplicationFiled: September 14, 2004Publication date: February 12, 2009Inventors: Jiunn-Liang Ko, Yu-Lu Huang, Tzu-Chih Chen, Hsu-Wei Hung, Ho-Lung Jiang, Ching-Lung Hu, Cheng-Chun Kuan, Hsuan-Ju Thou
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Publication number: 20090041889Abstract: The present invention relates to a gene encoding a protein having a vicinal diketone or diacetyl-reducing activity and use thereof, in particular, a brewery yeast for producing alcoholic beverages with superior flavor, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose capability of producing vicinal diketones, especially diacetyl, that are responsible for off-flavors in products, is reduced by amplifying expression level of MMF1 gene encoding a protein (Mmflp) having a vicinal diketone or diacetyl-reducing activity, especially the non-ScMMF1 gene or ScMMF1 gene specific to a lager brewing yeast, and to a method for producing alcoholic beverages with said yeast.Type: ApplicationFiled: November 21, 2006Publication date: February 12, 2009Applicant: SUNTORY LIMITEDInventors: Yoshihiro Nakao, Yukiko Kodama, Tomoko Shimonaga, Fumihiko Omura
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Publication number: 20090029001Abstract: The present invention relates to a gene encoding a protein responsible for flocculation property of yeast and use thereof, in particular, a brewery yeast with appropriate flocculation property for producing desired alcoholic beverages, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast to which suitable flocculation property for brewing desired alcoholic beverages was imparted by controlling expression level of KRE9 gene encoding a protein responsible for flocculation property of brewery yeast, especially non-ScKRE9 gene specific to a lager brewing yeast, to alcoholic beverages produced with said yeast and to a method for producing said beverages.Type: ApplicationFiled: December 27, 2006Publication date: January 29, 2009Applicant: Suntory LimitedInventors: Yoshihiro Nakao, Yukiko Kodama, Tomoko Shimonaga
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Publication number: 20090029455Abstract: Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in taste signaling, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for representing taste perception of a particular tastant in a mammal are also described, as are methods for generating novel molecules or combinations of molecules that elicit a predetermined taste perception in a mammal, and methods for simulating one or more tastes. Further, methods for stimulating or blocking taste perception in a mammal are also disclosed.Type: ApplicationFiled: October 23, 2007Publication date: January 29, 2009Applicant: Senomyx, Inc.Inventors: Jon Elliot Adler, Sergey Zozulya, Xiaodong Li, Shawn O'Connell, Lena Staszewski
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Publication number: 20090031454Abstract: The invention provides isolated nucleic acid molecules which encode novel fatty acid hydroxylases. The invention also provides recombinant expression vectors including hydroxylase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for the production of hydroxyl fatty acids such as 12-hydroxyoctadec-9-enoic acid (ricinoleic acid).Type: ApplicationFiled: June 22, 2007Publication date: January 29, 2009Applicants: Bioriginal Food & Science Corp., University of SaskatchewanInventors: Dauenpen Meesapyodsuk, Xiao Qiu
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Publication number: 20090011080Abstract: The present invention relates to a gene encoding a protein responsible for flocculation property of yeast and use thereof, in particular, a brewery yeast with appropriate flocculation property for producing desired alcoholic beverages, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast to which suitable flocculation property for brewing desired alcoholic beverages was imparted by controlling expression level of CIS3 gene encoding a protein responsible for flocculation property of brewery yeast, especially non-ScCIS3 gene specific to a lager brewing yeast, to alcoholic beverages produced with said yeast and to a method for producing said beverages.Type: ApplicationFiled: January 15, 2007Publication date: January 8, 2009Applicant: Suntory LimitedInventors: Yoshihiro Nakao, Yukiko Kodama, Tomoko Shimonaga
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Process For Producing Protein With Reduction of Acidic Sugar Chain and Glycoprotein Produced Thereby
Publication number: 20090004725Abstract: The present invention intends to find out a gene participating in addition of mannose phosphate to a sugar chain of a glycoprotein originating in a yeast belonging to the genus Pichia and provide a means of controlling the same. The present invention also intends to provide a process for producing a protein with reduction of an acidic sugar chain by using the thus controlled yeast strain belonging to the genus Pichia. Namely, the present invention includes a protein participating in the addition of mannose phosphate to a sugar chain of a glycoprotein; a gene coding this protein; a mutant of this gene; a vector carrying the mutant gene; a yeast strain belonging to the genus Pichia having been transformed by this vector; a process for producing a protein with reduction of an acidic sugar chain by using the transformed yeast strain; and a glycoprotein thus produced.Type: ApplicationFiled: August 1, 2008Publication date: January 1, 2009Applicant: MITSUBISHI PHARMA CORPORATIONInventors: Masami Miura, Masaaki Hirose, Taeko Miwa, Hiroyuki Irie, Shinobu Kuwae, Kenmi Miyano, Wataru Otani, Hideyuki Ohi -
Publication number: 20090004693Abstract: The present invention relates to polypeptides that have an activity corresponding to at least one activity of the SEC61 polypeptide, polynucleotides encoding these polypeptides and the use thereof in the preparation of host cells suitable for production of a polypeptide of interest. Such host cells may have an increased capacity to secrete a polypeptide of interest.Type: ApplicationFiled: June 16, 2005Publication date: January 1, 2009Inventors: Cornelis Maria Sagt, Noel Nicolaas Maria Elisabeth Peij, Linda De Lange, Martina Beishuizen, Serge Petrus Donkers
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Patent number: 7465850Abstract: The invention relates to methods for increasing the oil content in plants, preferably in plant seeds, by expressing a polypeptide from yeast. The invention furthermore relates to expression constructs for expressing the yeast polypeptide in plants, preferably in plant seeds, the transgenic plants expressing the yeast polypeptide and to the use of said transgenic plants for the production of food, feeds, seed, pharmaceuticals or fine chemicals, in particular for the production of oils.Type: GrantFiled: July 3, 2003Date of Patent: December 16, 2008Assignee: BASF Plant Science GmbHInventors: Martijn Gipmans, Anders Dahlqvist, Antoni Banas, Ulf Stahl, Eva Wiberg, Marit Lenman, Hans Ronne, Sten Stymne
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Publication number: 20080274500Abstract: The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.Type: ApplicationFiled: March 24, 2008Publication date: November 6, 2008Applicant: Novozymes, Inc.Inventors: Debbie Yaver, Peter Nham
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Publication number: 20080274959Abstract: The present invention provides a chromoprotein produced by Actinomadura sp. 21G792, as well as amino acid and nucleic acid sequences of the apoprotein component of the chromoprotein and of components of the biosynthetic pathway for the chromophore. The present invention is useful for developing pharmaceutical and treating diseases such as cancer or bacterial infections.Type: ApplicationFiled: December 16, 2005Publication date: November 6, 2008Applicant: WyethInventors: Bradley A. Haltli, Haiyin He, Ying Huang, Sridhar Krishna Rabindran, Jiang Wu, Min He
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Publication number: 20080261272Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.Type: ApplicationFiled: April 15, 2008Publication date: October 23, 2008Applicant: EISAI R&D MANAGEMENT CO., LTDInventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Ohba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Publication number: 20080263729Abstract: The present invention provides a MAP kinase homologue gene, designated EhHOG, isolated from Eurotium herbariorum, a common fungal species from the extreme hypersaline environment of the Dead Sea, capable of improving tolerance of plants and other organisms to abiotic stresses such as osmotic, heat, dehydration, freezing-thawing, oxidative and salinity stress.Type: ApplicationFiled: September 8, 2005Publication date: October 23, 2008Inventors: Eviatar Nevo, Yan Jin, Weining Song
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Publication number: 20080227145Abstract: The present invention relates to a method for increasing the efficiency of targeted integration of a polynucleotide to a pre-determined site into the genome of a filamentous fungal cell with a preference for NHR, wherein said polynucleotide has a region of homology with said pre-determined site, comprising steering an integration pathway towards HR. The present invention also relates to a mutant filamentous fungus originating from a parent cell, said mutant having an HR pathway with elevated efficiency and/or an NHR pathway with a lowered efficiency and/or a NHR/HR ratio with decreased efficiency as compared to said HR and/or NHR efficiency and/or NHR/HR ratio of said parent cell under the same conditions.Type: ApplicationFiled: March 31, 2005Publication date: September 18, 2008Inventors: Petrus Jacobus Theodorus Dekker, Marco Alexander van den Berg
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Publication number: 20080220473Abstract: The present invention discloses mannosyl-glycoprotein endo-beta-N-acetylglucosamidase (E.C.3.2.1.96, endo-N-acetyl-beta-D-glucosaminidase acting on the di-N-acetylchitobiosyl part of N-linked glycans) from filamentous fungi such as Trichoderma reesei.Type: ApplicationFiled: November 9, 2005Publication date: September 11, 2008Applicant: UNIVERSITEIT GENTInventors: Marc Claeyssens, Ingeborg Stals
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Publication number: 20080220484Abstract: The present invention relates to proteins having an enzymatic activity of reducing substituted alkanones such as 3-methylamino-1-(2-thienyl)-propan-1-one. The invention furthermore relates to nucleic acids coding for said proteins, nucleic acid constructs, vectors, genetically modified microorganisms and to methods for preparing optically active substituted alkanols, such as, for example, (S)-3-methylamino-1-(2-thienyl)-(S)-propanol.Type: ApplicationFiled: September 14, 2006Publication date: September 11, 2008Applicant: BASF AktiengesellschaftInventors: Michael Breuer, Thomas Friedrich, Maria Kesseler
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Publication number: 20080220503Abstract: Provided herein are a method for selecting a gene participating in the desire brewing character and compiling a database of the whole genome sequence of industrial yeast; identifying a gene participating in a brewing characteristic from the database; functional analysis of the gene; and a DNA array of the whole genome sequences of an industrial yeast. Also provided are a method for yeast breeding; a method of producing an alcoholic beverage with improved quality; and a screening method to identify genes that increase productivity and/or improve flavor in the production of an alcohol or an alcoholic beverage by (A) analyzing a whole industrial yeast genome sequence, (B) comparing the genome sequence with the genome sequence of S. cerevisiae, (C) selecting a gene of the industrial yeast encoding having 70 to 97% identity to an amino acid sequence of S. cerevisiae; and (D) analyzing the selected gene.Type: ApplicationFiled: August 7, 2006Publication date: September 11, 2008Inventors: Yoshihiro Nakao, Norihisa Nakamura, Yukiko Kodama, Tomoko Fujimura, Toshihiko Ashikari
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Publication number: 20080187964Abstract: The present invention relates to isolated promoter sequences, particularly a promoter isolated from Aspergillus niger designated herein as A4-L or A4 and DNA constructs and vectors including the same.Type: ApplicationFiled: April 2, 2008Publication date: August 7, 2008Inventors: Steve Kim, Edwin Lee, Wei Liu, Huaming Wang
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Publication number: 20080182321Abstract: A novel hybrid fungus culture, designated J9277, of the mushroom species Agaricus bisporus produces crops of mushrooms having white, rounded, thick-fleshed caps and proportionally long stems in a relatively short interval of time. Diverse additional strains can be developed from J9277 by various means including somatic and tissue culture selection, basidiospore selection, and hybridization to other strains of Agaricus bisporus, and the resulting derivative strains can be screened for desirable commercial characteristics.Type: ApplicationFiled: May 12, 2006Publication date: July 31, 2008Inventors: Richard W. Kerrigan, Jeffrey W. Smathers, Mark P. Wach
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Publication number: 20080166765Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, fungal genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. These genes encode proteins participating in fungal cell wall synthesis. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved.Type: ApplicationFiled: December 17, 2007Publication date: July 10, 2008Applicant: Eisai R&D Management, Ltd.Inventors: Kappei Tsukahara, Katsura Hata, Koji Sagane, Kazutaka Nakamoto, Mamiko Tsuchiya, Naoaki Watanabe, Fuminori Oba, Itaru Tsukada, Norihiro Ueda, Keigo Tanaka, Junko Kai
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Publication number: 20080161225Abstract: The present invention relates to an antifungal protein gene and cDNA sequence thereof, which is obtained by mining the whole genome sequences of Monascus pilosus BCRC 38072 and the unigene database. The gene can encode an antifungal protein MAFP1. A purified protein obtained from M. pilosus culture broth having molecular weight of about 7 kDa is identified as MAFP1 by N-terminal protein sequencing and comparative analysis. The purified MAFP1 protein can inhibit the growth of pathogens such as Paecilomyces variotii BCRC 33174 and Helminthosporium panici BCRC 35004. In addition, it is found by PCR test that the gene of this antifungal protein exists in other Monascus species such as M. Barkeri, M. floridanus, M. lunisporas, M. pilosus, M. ruber and the like. It is also been proved that the mafp1 gene and cDNA thereof in four Monascus strains, M. pilosus (BCRC 38072, BCRC 38093 and BCRC 31502) and M. ruber BCRC 31533, have the same DNA sequences.Type: ApplicationFiled: December 27, 2006Publication date: July 3, 2008Inventors: Ching-Yu Tu, Li-Ling Liaw
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Publication number: 20080145892Abstract: The present invention relates to the field of antibiotics, and more specifically to the isolation of nucleic acid molecules that code for the biosynthetic pathway of the glycopeptide antibiotic A40926. Disclosed are the functions of the gene products involved in A40926 production. The present invention provides biosynthetic genes that code for A40926 production, the encoded polypeptides, the recombinant vectors comprising the nucleic acid sequences that encode said polypeptides, the host cells transformed with said vectors and methods for producing glycopeptide antibiotics using said transformed host cells, including methods for producing A40926, a precursor thereof, a derivative thereof or a modified glycopeptide different from A 40926 or a precursor thereof.Type: ApplicationFiled: October 15, 2003Publication date: June 19, 2008Applicant: PfizerInventors: Stefano Donadio, Margherita Sosio, Fabrizio Beltrametti
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Publication number: 20080138873Abstract: The present invention relates to yeast strains with improved carbohydrate fermentation capacity, in particular to strains transformed with a gene encoding an improved hexose transporter gene, more in particular a mutated HXT3 gene.Type: ApplicationFiled: December 20, 2004Publication date: June 12, 2008Inventors: Patrice Jacques Marie Pellerin, Bruno Blondin, Jean-Marie Sablayrolles, Carole Guillaume
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Publication number: 20080118965Abstract: The invention relates to a nucleotide sequence comprising: a synonymous nucleotide coding sequence with optimized codon frequency such that a native codon has been exchanged with a synonymous codon, said synonymous codon encoding the same amino acid as the native codon and having a higher frequency in codon usage as defined in Table 1 than the native codon; and optionally said nucleotide sequence comprises control sequences such as: one translational termination sequence orientated in 5? towards 3? direction selected from the following list of sequences: TAAG, TAGA and TAAA, preferably TAAA, and/or one translational initiator coding sequence orientated in 5? towards 3? direction selected from the following list of sequences: gctnccyyc, using ambiguity codes for nucleotides: v (A/C/G); n (A/C/G/T), preferably 5?-GCT TCC TTC-3?.Type: ApplicationFiled: January 24, 2006Publication date: May 22, 2008Inventors: Johannes Andries Roubos, Serge Petrus Donkers, Hein Stam, Noel Nicolaas Maria Elisabeth Van Peij
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Patent number: 7351573Abstract: The present invention provides a novel ?-glucosidase nucleic acid sequence, designated bgl3, and the corresponding BGL3 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL3, recombinant BGL3 proteins and methods for producing the same.Type: GrantFiled: January 27, 2005Date of Patent: April 1, 2008Assignee: Genencor International, Inc.Inventors: Nigel Dunn-Coleman, Frits Goedegebuur, Michael Ward, Jian Yao
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Patent number: 7351568Abstract: The present invention provides a novel endoglucanase nucleic acid sequence, designated egl6, and the corresponding EGVI amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVI, recombinant EGVI proteins and methods for producing the same.Type: GrantFiled: January 10, 2006Date of Patent: April 1, 2008Assignee: Genencor International, Inc.Inventors: Nigel Dunn-Coleman, Frits Goedegebuur, Michael Ward, Jian Yao
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Publication number: 20080069829Abstract: A substantially pure, isolated, antigenic protein from fungi of the genus Malassezia, characterized in that said antigenic protein has a binding ability to IgE antibodies from patients with allergoses; an antigenic fragment derived from the antigenic protein; and an antibody against the antigenic protein or fragments thereof. According to the present invention, there can be provided an isolated and purified antigenic protein having high purity from Malassezia, antigenic fragments thereof, and a specific antibody against those antigenic protein or fragments thereof. In addition, there can be provided a diagnostic agent, a therapeutic agent, or a prophylactic drug for Malassezia allergoses, wherein the agent includes, as an active ingredient, the antigenic protein or fragments thereof.Type: ApplicationFiled: April 28, 2005Publication date: March 20, 2008Applicant: TAKARA BIO INC.Inventors: Kazutoh Takesako, Takashi Okado, Tomoko Yagihara, Masanobu Kuroda, Yoshimi Onishi, Ikunoshin Kato, Kazuo Akiyama, Hiroshi Yasueda, Hideyo Yamaguchi
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Patent number: 7332341Abstract: The present invention relates to mutants cells comprising a marker-free modification of a gene, and methods for obtaining and using such mutant cells.Type: GrantFiled: March 31, 2005Date of Patent: February 19, 2008Assignee: Novozymes, Inc.Inventors: John C. Royer, Lynne M. Christanson, Gregory A. Gambetta, Howard Brody, Suzanne M. Otani, Wendy T. Yoder
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Patent number: 7309595Abstract: The present invention provides An isolated polypeptide which has proline specific endoprotease activity, selected from the group consisting of:(a) a polypeptide which has an amino acid sequence which has at least 40% amino acid sequence identity with amino acids 1 to 526 of SEQ ID NO:2 or a fragment thereof;(b) a polypeptide which is encoded by a polynucleotide which hybridizes under low stringency conditions with (i) the nucleic acid sequence of SEQ ID NO:1 or a fragment thereof which is at least 80% or 90% identical over 60, preferably over 100 nucleotides, more preferably at least 90% identical over 200 nucleotides, or (ii) a nucleic acid sequence complementary to the nucleic acid sequence of SEQ ID NO:1.Type: GrantFiled: December 6, 2001Date of Patent: December 18, 2007Assignee: DSM IP Assets B.V.Inventors: Petrus Jacobus Theodorus Dekker, Luppo Edens, Robertus Antonius Mijndert Van Der Hoeven, Linda De Lange
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Patent number: 7309815Abstract: This invention encompasses the identification and isolation of genes that confer disease control properties in plants, as well as plants comprising such genes. These genes are derived from the following sources: Nicotiana benthamiana, Oryzae sativa (var. Indica IR7), Papaver rhoeas, Saccharomyces cerevisiae and Trichoderma harzianum (Rifai 1295-22). The control conferred is against the one or more of the following phytopathogens: Aspergillus flavus, Cercospora zeae-maydis, Fusarium monilforme, Fusarium graminearum, Helminthosporium maydis, Phoma lingam, Phomopsis helianthi, Phytopthera infestans, Pyricularia oryzae, Pythium ultimum, Rhizoctonia solani, Sclerotinia sclerotiorum, Ustilago maydis, and Verticillium dahliae. Further, this invention encompasses other homologous and heterologous sequences with a high degree of functional similarity.Type: GrantFiled: August 30, 2002Date of Patent: December 18, 2007Assignee: Dow AgroSciences LLCInventors: Vipula Kiran Shukla, Holly Jean Butler, Aaron Todd Woosley, Pamela Rene Haygood, Cristie Min Dewes, James Patrick Connell, Ignacio Mario Larrinua, Avutu Sambi Reddy, Anthony Trieu, Zihua Hu
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Patent number: 7285407Abstract: The purpose of the present invention is to isolate a gene encoding novel exo-, or endo-?-1,3-glucanase, to obtain a microorganism having an enhanced expression of said gene and to degrade ?-1,3-glucan to its low molecular weight form by means of said enzymes. The present invention relates to a gene or DNA encoding novel enzymes having ?-1,3-glucanase activity (exo-, or endo-?-1,3-glucanase), a recombinant expression vector comprising them, a microorganism having the recombinant expression vector, the novel enzymes having ?-1,3-glucanase activity, and a method for the production of said enzymes.Type: GrantFiled: March 22, 2005Date of Patent: October 23, 2007Assignees: National Institute of Advanced Industrial Science and Technology, National Institute of Technology and EvaluationInventors: Masayuki Machida, Motoaki Sano, Misao Sunagawa, Tasuku Nakajima, Keietsu Abe, Katsuya Gomi, Kiyoshi Asai, Taishin Kin, Hideki Nagasaki, Akira Hosoyama, Osamu Akita, Naotake Ogasawara, Satoru Kuhara
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Patent number: 7279316Abstract: The invention relates to glycosidases and to polynucleotides encoding the glycosidases. In addition methods of designing new glycosidases and method of use thereof are also provided. The glycosidases have increased activity and stability at increased pH and temperature.Type: GrantFiled: March 6, 2002Date of Patent: October 9, 2007Assignee: Verenium CorporationInventors: Jay M. Short, Edward Bylina, Ronald V. Swanson, Eric J. Mathur, David E. Lam
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Patent number: 7276489Abstract: The invention relates to the therapeutic use of oligonucleotides as immunostimulatory agents in immunotherapy applications. More particularly, the invention provides immunomers for use in methods for generating an immune response or for treating a patient in need of immunostimulation. The immunomers of the invention comprise at least two oligonucleotides linked at their 3? ends, internucleoside linkages or functionalized nucleobase or sugar to a non-nucleotidic linker, at least one of the oligonucleotides being an immunostimulatory oligonucleotide and having an accessible 5? end.Type: GrantFiled: October 24, 2002Date of Patent: October 2, 2007Assignee: Idera Pharmaceuticals, Inc.Inventors: Sudhir Agrawal, Ekambar R. Kandimalla, Dong Yu, Lakshmi Bhagat
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Patent number: 7273742Abstract: The present invention provides a novel ?-glucosidase nucleic acid sequence, designated bgl3, and the corresponding BGL3 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL3, recombinant BGL3 proteins and methods for producing the same.Type: GrantFiled: May 16, 2005Date of Patent: September 25, 2007Assignee: Genencor International, Inc.Inventors: Nigel Dunn-Coleman, Frits Goedegebuur, Michael Ward, Jian Yao
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Patent number: 7264964Abstract: Chimeric polypeptides are disclosed that comprise a first polypeptide segment having histone acetyltransferase enzymatic activity and a second polypeptide segment that is similar to a subunit of a chromatin-associated histone deacetyltransferase protein complex. Also disclosed are nucleic acids encoding such chimeric polypeptides and eukaryotic organisms expressing such chimeric polypeptides.Type: GrantFiled: June 21, 2002Date of Patent: September 4, 2007Assignee: Ceres, Inc.Inventors: Van-Dinh Dang, Jack Okamuro
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Patent number: 7250502Abstract: The present invention provides formaldehyde dehydrogenase genes (FLD) from methylotrophic yeasts. The FLD structural genes confer resistance to formaldehyde and are therefore useful as a selectable marker in methylotrophic yeasts. The FLD promoter sequences are strongly and independently induced by either methanol as sole carbon source (with ammonium sulfate as nitrogen source) or methylamine as sole nitrogen source (with glucose as carbon source). Induction under either methanol, methylamine or both provides levels of heterologous gene expression comparable to those obtained with the commonly used alcohol oxidase I gene promoter (PAOX1). The FLD promoter of Pichia pastoris (PFLD1) is an attractive alternative to PAOX1 for expression of foreign genes in P. pastoris, allowing regulation by carbon (methanol) or nitrogen (methylamine) source within the same expression strain.Type: GrantFiled: June 12, 2003Date of Patent: July 31, 2007Assignee: Research Corporation Technologies, Inc.Inventor: James M. Cregg
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Patent number: 7244821Abstract: The invention relates to yeast strains in which a human GLUT4 transport or a human GLUT1 transporter can be functionally expressed and to particular GLUT4 transport proteins which can be functionally expressed particularly readily in yeast strains.Type: GrantFiled: September 10, 2003Date of Patent: July 17, 2007Assignee: Sanofi-Aventis Deutschland GmbHInventors: Guenter Mueller, Silke Dlugai, Doerthe Voss, Eckhard Boles
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Patent number: 7235715Abstract: Disclosed are transgenic plants containing an exogenous nucleic acid encoding an L3 protein. The plant exhibits increased resistance to viruses and/or fungi that infect plants. The L3 proteins include wild-type proteins, spontaneously occurring mutants and non-naturally occurring L3 mutants. Also disclosed are methods of reducing the toxicity of single-chain ribosome inhibitory proteins in cells, e.g., yeast, plant and animal cells, by co-administering the L3 protein with the RIP. Further disclosed are non-naturally occurring L3 mutants that (a) substantially fail to bind single-chain RIPs that bind endogenous L3 proteins, (b) are unable to maintain M1 killer virus, (c) promote altered programmed ribosomal frameshift efficiency, (d) exhibit resistance to peptidyltransferase inhibitors, and combinations of any of (a)–(d).Type: GrantFiled: August 7, 2003Date of Patent: June 26, 2007Assignees: Rutgers, The State University, The University of Medicine and Dentistry of New JerseyInventors: Nilgun E. Tumer, Jonathan D. Dinman, Katalin A. Hudak
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Patent number: 7226767Abstract: A substantially pure culture of a microorganism having enzyme activities of pyrG and CCT and carrying a recombinant DNA composed of a DNA fragment containing genes encoding pryG and CCT and a vector.Type: GrantFiled: April 2, 2002Date of Patent: June 5, 2007Assignee: Kyowa Hakko Food Specialities Co., Ltd.Inventors: Akihiko Maruyama, Tatsuro Fujio, Sadao Teshiba
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Patent number: 7217538Abstract: Disclosed and claimed is the CaESS1 gene, portions thereof such as primers or probes, expression products therefrom, and methods for using the gene, and expression products; for instance, for diagnostic, therapeutic or preventive compositions.Type: GrantFiled: January 15, 2003Date of Patent: May 15, 2007Assignee: Health Research IncorporatedInventors: Steven D Hanes, Gina Devasahayam, Vishnu Chaturvedi
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Patent number: 7214491Abstract: The present invention relates to a ?12 fatty acid desaturase able to catalyze the conversion of oleic acid to linoleic acid (LA; 18:2). Nucleic acid sequences encoding the desaturase, nucleic acid sequences that hybridize thereto, DNA constructs comprising the desaturase gene, and recombinant host microorganisms expressing increased levels of the desaturase are described. Methods of increasing production of specific ?-3 and/or ?-6 fatty acids are described by overexpression of the ?12 fatty acid desaturase or by disruption of the native gene.Type: GrantFiled: May 6, 2004Date of Patent: May 8, 2007Assignee: E. I. du Pont de Nemours and CompanyInventors: Narendra S. Yadav, Hongxiang Zhang
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Patent number: 7208300Abstract: The present invention relates to a novel lysyl oxidase genes, termed EER-7. The invention relates to the protein and nucleic acids encoding the protein. The invention further relates to an assay system to identify compounds that selectively modulate EER-7 protein activity by interaction with estrogen receptors.Type: GrantFiled: August 8, 2001Date of Patent: April 24, 2007Assignee: WyethInventors: Mark J. Evans, Marshall S. Scicchitano, Ashok R. Bapat, Ramesh A. Bhat, Robert Mastroeni, Sotirios K. Karathanasis
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Patent number: 7205123Abstract: The present invention provides recombinant DNA comprising a transcription promoter and a downstream sequence to be expressed, in operable linkage therewith, wherein the transcription promoter comprises a region found upstream of the open reading frame of a highly expressed Phaffia gene, preferably a glycolytic pathway gene, more preferably the gene coding for Glyceraldehyde-3-Phosphate Dehydrogenase. Further preferred recombinant DNAs according to the invention contain promoters of ribosomal protein encoding genes, more preferably wherein the transcription promoter comprises a region found upstream of the open reading frame encoding a protein as represented by one of the amino acid sequences depicted in any one of SEQIDNOs: 24 to 50.Type: GrantFiled: October 30, 2001Date of Patent: April 17, 2007Assignee: DSM IP Assets B.V.Inventors: Albert Johannes Joseph Van Ooijen, Jan Cornelis Verdoes, Jan Wery