Abstract: The present invention provides a highly versatile method for amino acid analysis, the method enabling separation and analysis of amino acids in a sample with high precision in a shorter time. This method is a method for amino acid analysis, the method including a step of allowing a sample containing a plurality of amino acids to flow together with an eluent 2 through a separation column 4 packed with a cation exchange resin, thereby separating the amino acids, and a step of detecting the separated amino acids, wherein the eluent 2 is an eluent containing a divalent or higher inorganic acid, a cation source, and water, and having a pH of 5.0 or lower, and the sample is allowed to flow through the separation column 4 heated by applying a temperature gradient including a temperature region of 100° C. or higher.

Abstract: A simulated moving-bed type chromatographic separation method separating a weakly adsorptive component, a strongly adsorptive component, and an intermediately adsorptive component, with eluents by using a circulation system in which a plurality of unit packed columns packed with an adsorbent are connected in series and in an endless form via pipes in which a feed solution supply port F, two or more eluent supply ports D corresponding to the eluents, an extraction port A of a fraction containing the weakly adsorptive component, an extraction port B of a fraction containing the intermediately adsorptive component, and an extraction port C of a fraction containing the strongly adsorptive component are provided in the pipes of the circulation system, and positions of the ports F, A, B, and C are set to have a specified relationship.

Abstract: Methods for achieving complete sequence coverage of monoclonal antibodies by trypsin digestion and dual-column LC-MS system are provided. The disclosed method improves upon current techniques for standard peptide mapping.

Abstract: An alternating flow column chromatography apparatus comprising a ‘U’ shaped or T shaped separation column including at least one loading port for loading of components for separation, a first purification column in fluid communication with one end of the separation column and a second purification column in fluid communication with another end of the separation column, at least one eluent input port, an eluate output port and an alternating flow valve in fluid communication with the primary eluent input port, the eluate output port, the first purification column and the second purification column wherein, when operated, the alternating flow valve reverses the flow of eluent through the purification columns and the separation column. Also a method of using the apparatus. A benefit of the apparatus and method is more efficient operation compared to existing direct flow column chromatography apparatuses.

Abstract: A separation column connecting device includes: a column holder for retaining a separation column; a first fitting holder carrying a first fitting which includes a seal portion to be connected to an upstream seal portion of the separation column and connected with an upstream pipe; a second fitting holder carrying a second fitting which includes a seal portion to be connected to a downstream seal portion of the separation column and connected with a downstream pipe; a body member to which either one of the first fitting holder and the second fitting holder is fixed; a driver for moving, relative to the body member, the first fitting holder or the second fitting holder not fixed to the body member; a guide for guiding the column holder in a direction of movement driven by the driver; and an elastic body disposed between the column holder and the second fitting holder.

Abstract: Methods for achieving complete sequence coverage of monoclonal antibodies by trypsin digestion and dual-column LC-MS system are provided. The disclosed method improves upon current techniques for standard peptide mapping.

Abstract: A system and method of reducing chromatographic band broadening within a separation column include passing a mobile phase through a length of a separation column, and generating a spatial thermal gradient external to and along the length of the separation column. The spatial thermal gradient is specifically configured to counteract a particular change in a property of the mobile phase as the mobile phase passes through the separation column. For example, the particular change counteracted may be a change in density or in temperature of the mobile phase. For analytical-scale columns, for example, the spatial thermal gradient may be configured to produce temperatures external to and along the length of the separation column that substantially matches temperatures predicted to form in the mobile phase along the column length as the mobile phase passes through the separation column, thereby substantially preventing formation of a radial thermal gradient in the mobile phase.

Abstract: A polymer-based PLOT capillary column prepared by in situ copolymerization of a functional monomer and a crosslinking monomer, which enhances the strength of the polymer matrix, is disclosed. Also disclosed is a system comprising the polymer-based PLOT column coupled to a mass flow or concentration sensitive detector, for carrying out a chemical analysis method on samples separated by liquid chromatography using the column, and a process for using the system. Columns of the invention can be prepared in a robust fashion with a very narrow i.d., e.g., 5-15 ?m. Thus, they are suitable for commercial use in ultratrace LC/MS proteomic analysis. Columns according to the invention are characterized by high resolving power and high column-to-column reproducibility. When these columns are coupled on-line with, e.g., ESI-MS detection, the resulting systems are capable of detecting the component parts of complex proteomic samples down to the low attomole to sub-attomole level.

Abstract: A column oven includes a main unit, an auxiliary unit, an outside sensor, an internal sensor, a temperature range holding section for holding a plurality of temperature ranges, a temperature range specifying portion for specifying the temperature range to which the outside temperature belongs, a temperature control program holding section for holding a temperature control program for a control method of the main unit and the auxiliary unit in each temperature range in such a way that outputs of the main unit and the auxiliary unit are continuous in the temperature ranges that are adjacent to each other, a control method setting portion for setting a control method of the main unit and the auxiliary unit based on the temperature range specified and the temperature control program, and a temperature control portion for controlling the main unit and the auxiliary unit.

Abstract: The present invention provides a rack for holding sample vials comprising: (i) a solid base; (ii) an upwardly extending wall around the perimeter of said base; (iii) a plurality of means for supporting a plurality of vials, each means for supporting one vial; and (iv) a means for draining liquid from said rack.

Abstract: Disclosed are an apparatus and method for separation analysis of mannose-6-phosphate (M6P) by post-column fluorescence detection method. The apparatus is based on chromatography, and includes a column with a solid phase having affinity for phosphate, a flow path for the eluate, a heater installed on the flow path for M6P and a basic amino acid to react by heating the eluate in the flow path, and a fluorescence detector installed downstream of the heater for continuously irradiating the eluate with excitation light and measuring the intensity of the emission, and may include in the flow path a supply channel for addition of a basic amino acid between the column and the heater. The method is characterized in that it uses the apparatus and a second mobile phase consisting of a second buffer containing phosphate of predetermined concentration and adjusted to a predetermined pH.

Abstract: An automated system for handling components of a chromatographic system, comprising: one or more trap columns each having sorbent material therein; a robotic system for moving a trap column from a storage position to an operating position where the trap column is connected in a liquid pathway along which solvent is flowed in operation to a liquid chromatography (LC) column; and an optical sensor for sensing one or more parameters of the trap column. Sensed parameters can include: a presence or absence of a trap column in the operating position, a position or orientation of the trap column, a fluid level in the trap column, the presence and position of a sorbent material in the trap column, the condition of the trap column and/or whether the trap column is new or used.

Abstract: Two quantitative separation approaches to fractionate de-asphalted oils (DAOs) into seven classes of compounds (saturates, 1-4+ ring-aromatics, sulfides, and polars). In the first step (named as “SGS”) of present invention, the DAO of a petroleum vacuum resid is separated in to four classes of compounds, namely saturates, aromatics, and sulfides. In this first step of separation, about 3 grams of a DAO can be separated. Whereas in the second step (named as “ARC” separation) of invention, only less than 300 mg of the aromatic fraction obtained in “SGS” (described above) can be further fractionated at very low temperature (about ?40 degrees centigrade) into 4 fractions, namely 1-ring, 2-ring, 3-ring, and 4+-ring aromatics. The present invention protocol is suitable for a wide range of compositionally different DAOs of petroleum vacuum resids.

Abstract: Embodiments of the present invention are directed to devices, methods and apparatus for performing separations in which particulates are separated or removed from a fluid stream flowing to a column. In one embodiment directed to a device, the invention includes a body (13) having a first surface (17) and a second surface (19) and at least one wall (23). At least one of the first surface (17) and second surface (19) is for receiving in sealing engagement a first conduit means. The remaining first surface (17) or second surface (19) is for engaging a column or a second conduit (19). The at least one wall (23) extends between the first surface (17) and the second surface (19). The body (13) has at least one opening (25) extending from the first surface (17) to the second surface (19) defining a chamber for receiving a solid phase separation media assembly (15).

Abstract: A chromatography column comprises a pipe that contains a tubular metal casing with an inlay and sealing ring. The inlay is configured as a plastics material tube and is pushed or drawn into the metal casing and a sealing ring of plastics material is connected to the inlay at the end. Because of this structure, a connection to further system parts is possible, which is carry-over-free, inert on the inside, liquid-tight, optionally gas-tight, and pressure-tight. A connection element and connection connect the pipe to feed and/or discharge lines of a capillary system. Pipes, connection elements and connections of this type are advantageously used in low and high pressure systems, such as, for example, in an HPLC column. The use of such systems is advantageous in in-vitro diagnostics and in liquid handling applications.

Abstract: The invention relates to a system for packing chromatography columns with a chromatography medium and packing method for use in such columns. In particular, the invention relates to a method and system for packing chromatography columns which utilizes an external drive means to compress a bed of particulate medium to a target bed height.

Abstract: A method and system for conducting chromatographic analysis of a total vacuum resid to provide quantification of eight classes of compounds (i.e., asphaltenes, saturates, 1-4+ ring aromatics, sulfides, and polars) contained within the total vacuum resid without prior de-asphalting are disclosed. The system is also capable of conducting chromatographic analysis of a vacuum gas oils and de-asphalted oils to provide quantification of seven classes of compounds (i.e., saturates, 1-4+ ring aromatics, sulfides, and polars). The system is also capable of conducting chromatographic analysis of a resid to identity the presence of and provide quantification of asphaltenes.

Abstract: The invention relates to a method for separating fractions of a mixture to be separated including the steps of providing a device comprising at least two chromatography columns mounted in series, discontinuously injecting a mixture to be separated, in a single point of the device, discontinuously drawing off the fraction enriched in the product of interest, in a single point of the device, and injecting eluents in each column and displacing the points for injection eluents, the eluents having a different eluting power. The method allows efficient separation which is simple to perform.

Abstract: Systems and methods for monitoring analytes in real time using integrated chromatography systems and devices. Integrated microfluidic liquid chromatography devices and systems include multiple separation columns integrated into a single substrate. Using such a device, parallel analysis of multiple samples can be performed simultaneously and/or sequential analysis of a single sample can be performed simultaneously on a single chip or substrate. The devices and systems are well suited for use in high pressure liquid chromatography (HPLC) applications. HPLC chips and devices including embedded parylene channels can be fabricated using a single mask process.

Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

Abstract: An analytical instrument fitting assembly for coupling first and second analytical fluid conduits is provided. The fitting assembly includes a first fitting defining a central passage adapted to receive a first fluid conduit defining an internal diameter of no greater than 0.040 inch and a second fitting. The second fitting defines a central passage extending between first and second ends and is in fluid communication with the first fitting. A separation device is disposed within a central passages of the first and second fittings to selectively separate liquids passing therethrough. The fitting assembly further includes a retractable end fitting having a housing, a tube extending within the housing, and a biasing member. Selective positioning of the tube within the housing minimizes dead space between the tube and the second end.

Abstract: A fitting assembly includes a first fitting that is configured to receive a first fluid tube and a second fitting that is configured to receive a second fluid tube. The first fitting defines a first groove. The second fitting includes a spring that is configured to engage the first groove to connect the first and second fittings such that the first and second fluid tubes are placed in fluid communication and a fluid tight seal is established between the first and second sealing fittings.

Abstract: A polymer-based PLOT capillary column prepared by in situ copolymerization of a functional monomer and a crosslinking monomer, which enhances the strength of the polymer matrix, is disclosed. Also disclosed is a system comprising the polymer-based PLOT column coupled to a mass flow or concentration sensitive detector, for carrying out a chemical analysis method on samples separated by liquid chromatography using the column, and a process for using the system. Columns of the invention can be prepared in a robust fashion with a very narrow i.d., e.g., 5-15 ?m. Thus, they are suitable for commercial use in ultratrace LC/MS proteomic analysis. Columns according to the invention are characterized by high resolving power and high column-to-column reproducibility. When these columns are coupled on-line with, e.g., ESI-MS detection, the resulting systems are capable of detecting the component parts of complex proteomic samples down to the low attomole to sub-attomole level.

Abstract: Described is a capillary column cartridge. The cartridge can be used to perform separations according to various techniques such as capillary gas chromatography, capillary electrophoresis and capillary liquid chromatography. The cartridge includes a capillary column secured in a cartridge body. The capillary column includes an inlet port and an outlet port that, in some embodiments, are disposed on a planar surface of the body. When the body is engaged to a separation system module, the inlet port is aligned to receive a sample to be separated and the outlet port is aligned to provide the separated sample to the separation system module. The path of the capillary through the body has a non-planar path shape such as a coil shape. Consequently, longer column lengths can be accommodated, leading to an improvement in separation resolution. The body can include a material having a high thermal conductivity to achieve improved thermal performance.

Abstract: An object of the present invention is to provide a column for liquid chromatography that can be produced at low cost and allows suppression of non-specific adsorption of components derived from living organisms. Another object of the present invention is to provide a method for analyzing hemoglobin using the column for liquid chromatography. The present invention provides a column for liquid chromatography having a column pipe and an end fitting, wherein the column pipe and the end fitting comprise polyethylene or polymethylmethacrylate.

Abstract: Ionic liquid (IL)-mediated sol-gel hybrid organic-inorganic materials present enormous potential for effective use in analytical microextraction. One obstacle to materializing this prospect arises from high viscosity of ILs significantly slowing down sol-gel reactions. A method was developed which provides phosphonium-based, pyridinium-based, and imidazolium-based IL-mediated advanced sol-gel organic-inorganic hybrid materials for capillary microextraction. Scanning electron microscopy results demonstrate that ILs can serve as porogenic agents in sol-gel reactions. IL-mediated sol-gel coatings prepared with silanol-terminated polymers provided up to 28 times higher extractions compared to analogous sol-gel coatings prepared without any IL in the sol solution.

Abstract: A mobile phase supply device comprises an aqueous path including a first delivery pump for delivering an aqueous mobile phase, an organic solvent path including a second delivery pump for delivering an organic solvent mobile phase, and a mixer for mixing mobile phases from the aqueous path and the organic solvent path, and supplying the mixture to an analysis path of a liquid chromatograph. A flow resistance between the second delivery pump and the mixer is greater than a flow resistance between the first delivery pump and the mixer.

Abstract: There is provided a method for thermally focusing the elution times of compounds of a liquid chromatography system comprising a pre-column and a separation column as well as to provide a liquid chromatography system, wherein the chromatographic separation of a mixture of substances is optimized.

Abstract: This invention provides for the fully automated, hands free, packing of chromatography columns by means of delivering a pre-calculated volume of slurry and using two different packing modalities to stop the packing when either 1) this volume has been delivered in the column, or 2) when the adapter is moved to reach a bed height corresponding to the pre-calculated volume. Thus, a chromatography column can be packed in a fully automated fashion and such a column is 1) stable and 2) has the desired performance characteristic.

Abstract: After a target compound is captured by an adsorbent within a trap column (7) held by a column rack (8) in a substantially vertical position and the column is filled with water to wash its inside, dichloromethane is slowly introduced from an inlet end (7a) on the lower side by a pump (5). The water within the trap column (7) is pushed upward by dichloromethane and exits from an outlet end (7b), to be disposed of via a two-way selector valve (12) and a disposal passage (14). Dichloromethane elutes the captured target compound. Therefore, when the eluate, which initially consists of water, changes via an emulsion to dichloromethane, the eluate contains an adequately high concentration of target compound. At this point, the two-way valve (12) is switched to a preparative separation passage (13). As a result, a solution that is adequately free from water and yet contains the target compound can be collected into a collection container (17), and the target compound in solid forms can be quickly obtained.

Abstract: An injection device (100) comprising: a housing (102) defining a space (104); a seat (110) located in said space (104), the seat (110) being configured for receiving a flow path element (116) capable of injecting a pressurized fluid into the injection device (100) through the seat (110); a pressure source (132) for pressurizing a medium in said space (104), thereby reducing the pressure difference between the pressurized fluid in said flow path element (116) and said medium.

Abstract: A flow through cartridge for solid phase extraction of an analyte from a liquid has a cartridge body bounding a passage for retaining a sorbent. The passage extends from a first end face to a second end face, opposite the first end face of the cartridge body. The cartridge body is composed of a core of a first material extending around the passage and sealing rings of a second material, extending around an axis of the passage at the first and second end faces. Surface portions of the sealing rings constituting surface portions of the first and second end faces. The first material is stronger and stiffer than the second material.

Abstract: A concentration sensor device includes a sensor unit, a substrate, and a sedimentation limit unit. The sensor unit detects a concentration of a specific component contained in liquid. The substrate has a face to which the sensor unit is arranged. The sedimentation limit unit is integrally arranged with the sensor unit or arranged at an upstream side of the sensor unit in a flowing direction of the liquid. The sedimentation limit unit is configured to prevent sedimentation of a foreign object on the sensor unit. The sedimentation limit unit includes a piezoelectric element to vibrate when electricity is supplied so as to promote the foreign object to be separated from the sensor unit. The substrate has a recess recessed in a thickness direction of the substrate.

Abstract: Systems and methods for monitoring analytes in real time using integrated chromatography systems and devices. Integrated microfluidic liquid chromatography devices and systems include multiple separation columns integrated into a single substrate. Using such a device, parallel analysis of multiple samples can be performed simultaneously and/or sequential analysis of a single sample can be performed simultaneously on a single chip or substrate. The devices and systems are well suited for use in high pressure liquid chromatography (HPLC) applications. HPLC chips and devices including embedded parylene channels can be fabricated using a single mask process.

Abstract: A high pressure liquid chromatography (HPLC) capillary column device, system and method for processing a HPLC sample with a cartridge housing a packed capillary column; at least one inlet connection to the capillary column for a sample fluid; and at least one outlet connection from the capillary column for the sample fluid. The outlet connection is able to accommodate either a spray tip for atomizing the sample fluid or a transport tube for transporting the sample fluid from a spray tip column to a spray tip. Inlet connections enable supply of electrical power to the capillary column through electrical connections disposed within the cartridge housing; and gas for evaporating the sample liquid is supplied to at least one outlet connection from the capillary column for the sample fluid through a gas supply line within the cartridge housing. The temperature of the sample liquid can be controlled through a heat connection.

Abstract: A micro-fabricated chromatography column (70) which is particularly well-suited to the surface well-site and/or the downhole analysis of subterranean reservoir fluids in oilfield or gasfield applications (but which may also be used in non-oilfield or non-gasfield situations) is described. This micro-fabricated column integrates a micro-structured substrate (50), such as a silicon substrate, with a stationary phase material (66) deposited by sputtering as a coating in a microchannel (56) in the substrate (50). Benefits of the presently claimed and disclosed inventive concept(s) include enhanced separation of alkanes and isomers, particularly below hexane (i.e., below C6 as well as the separation of carbon dioxide, hydrogen sulfide, and water and other substances present in reservoir fluids, such as natural gas.

Abstract: To provide a column packing, a separation column, an analysis device, and an analysis method to reduce the analysis time without deteriorating the separation of multi-component amino acids. Disclosed are a column packing comprising a core portion made of a styrene-di-vinylbenzene copolymer having a cross-linkage degree of 50% or more and a shell portion made of a styrene-di-vinylbenzene copolymer having a cross-linkage degree of 20% or less; a separation column using the column packing; and a high-speed liquid chromatography analysis device and a analysis method using the separation column.

Abstract: Chromatography columns are disclosed. Methods of making chromatography columns and methods of using chromatography columns are also disclosed.

Abstract: Methods and systems for evaluating and/or monitoring chromatography column performance are provided. Embodiments apply multivariate analysis (MVA) methods to process data as well as transition analysis data to provide a comprehensive evaluation of chromatography column performance. In embodiments, transition analysis data generated over extended periods of time can be analyzed together with process data to evaluate column performance. Further, embodiments enable a compact and robust tool for combining and presenting performance evaluation results, which allows for time-efficient performance examination.

Abstract: Two quantitative separation approaches to fractionate de-asphalted oils (DAOs) into seven classes of compounds (saturates, 1-4+ ring-aromatics, sulfides, and polars). In the first step (named as “SGS”) of present invention, the DAO of a petroleum vacuum resid is separated in to four classes of compounds, namely saturates, aromatics, and sulfides. In this first step of separation, about 3 grams of a DAO can be separated. Whereas in the second step (named as “ARC” separation) of invention, only less than 300 mg of the aromatic fraction obtained in “SGS” (described above) can be further fractionated at very low temperature (about ?40 degrees centigrade) into 4 fractions, namely 1-ring, 2-ring, 3-ring, and 4+-ring aromatics. The present invention protocol is suitable for a wide range of compositionally different DAOs of petroleum vacuum resids.

Abstract: A column for chromatography includes a flow path for allowing a mobile phase, the flow path including mutually-opposing sidewalls and a bottom wall joined to the sidewalls and arranged at bottom ends of the sidewalls; and a plurality of pillars provided to extend along the sidewalls from the bottom wall and regularly arranged at a specified interval. The column is configured to separate individual components from a mixture sample containing multiple kinds of components by using the pillars as a stationary phase. Each of the pillars includes a curved outer surface and the sidewalls partially include a curved surface conforming to the outer surface of each of the pillars. The shortest distance between each of the sidewalls and a pillar positioned closest to said each of the sidewalls is equal to the shortest distance between the pillars in a flow direction of the mobile phase.

Abstract: A high performance liquid chromatography method to routinely and reproducibly detect and quantitate metal complexes is provided. The metal complexes used in the method of the invention can be different metal complexes, or they can be stereoisomers of the same metal complexes. The high performance liquid chromatography method of the present invention is suitable for the separation of diastereomers of the same metal complexes. Also provided is a chiral high performance liquid chromatography method to separate enantiomers of metal complexes. Superoxide dismutase mimetic compounds are also provided.

Abstract: Described is a method of transferring a chromatographic method between liquid chromatography (LC) systems. The method is based on a determination of an isoretention temperature at which two solutes co-elute. The method enables separations to be performed using different LC systems with reproducible and equivalent results. For example, the method allows for a chromatography method developed for HPLC to be more readily transferred to a UPLC system and for a chromatography method developed for a UPLC system to be more readily transferred to a HPLC system. The method addresses LC systems having column ovens of different design in which the internal column temperatures are not equal although the operating temperatures of the column ovens may be accurately controlled to equal values. The retention behavior and resolution of different LC systems is caused to be substantially the same so that equivalent separation results are obtained.

Abstract: A separation body for three-dimensional chromatography, which provides at least one predefinable individual retention capacity in each spatial direction, for an analyte transported in a mobile phase in the respective direction.

Abstract: Methods and systems for analyzing samples using multi-dimensional chromatography are disclosed.

Abstract: Solvent feed systems are disclosed. Chromatography systems containing a solvent feed system are also disclosed. Methods of making and using solvent feed systems are further disclosed.

Abstract: Automated sample injection apparatus, multiport valves, and chromatography systems containing an automated sample injection apparatus and/or a multiport valve are disclosed. Methods of making and using automated sample injection apparatus and multiport valves within chromatography systems are also disclosed.

Abstract: Chromatography columns are disclosed. Methods of making chromatography columns and methods of using chromatography columns are also disclosed.

Abstract: Chromatography assembly for multi-dimensional chromatography, includes a first separating column (12; 112) for separating a sample into components; a second separating column (13; 114) having different separating characteristics for further separating the components separated in the first separating column (12; 112); a collecting volume (20; 120, 121) for collecting the components from the first separating column (12; 112) before entering the second separating column (14; 114); a switching assembly (22, 24; 122, 124) for switching from a first state (FIG. 1; FIG. 3), where the components leaving the first separating column (12; 112) are collected in the collecting volume (20; 120, 121) to a second state (FIG. 2; FIG. 4) where the components collected in the collecting volume (20; 120, 121) are transferred to the second separating column (14; 114); and a detector (18; 118) for detecting sample components leaving the second separating column (14; 114).

Abstract: A method for determining the concentration of a chromatographic medium in a liquid slurry comprises the steps of: providing a column (1) having a top end (3) and a bottom end (2) and adapted to retain a chromatographic medium therein; introducing a defined volume of the slurry (15) into the column (1); providing a liquid flow through the column (1) from the top end to the bottom end of the column to settle the chromatographic medium in the column; measuring the bed height of the consolidated chromatographic medium or a value related thereto; and determining from the measured bed height or value related thereto the concentration, or a value related to the concentration of the chromatographic medium in the slurry. A device for performing the method as well as the use of the method in packing of especially large scale columns are also disclosed.