Abstract: A method of preparing a sugar chain sample, for reducing unreacted labeling reagent in a sample solution containing a labeled sugar chain, includes (process 1) a process of bringing the sample solution containing the labeled sugar chain into contact with monolithic silica, so as to allow the monolithic silica to adsorb a sugar chain component; (process 2) a process of washing the monolithic silica with a washing liquid; and (process 3) a process of bringing the monolithic silica into contact with an eluant, so as to elute the adsorbed sugar chain.

Abstract: A process and an apparatus for isotope ratio analysis, the process having the following steps: performing a liquid chromatography process and thus providing an eluate which comprises at least one liquid carrier fluid and at least one analytes, collecting a portion of interest from the eluate, processing the eluate portion to form at least one gaseous conversion products of the analytes, and supplying the gaseous conversion products, especially with gaseous carrier fluid, to an isotope analyzer and determining the isotrope ratios.

Abstract: The invention relates to the determination of phosphorylation levels of some specific salivary peptides (statherin, histatin 1, acidic proline-rich proteins, both in whole form (PRP-1 type) and in truncated form (PRP-3 type) as a non-invasive diagnostic marker with a >40% sensitivity for defining autism spectrum disorders (ASDs) patients, methods for performing said determination, a kit for the non-invasive diagnosis of at least 40% of autism cases, containing means for such a determination, and the use of such a determination for therapeutical research on autism.

Abstract: A method for determining asphaltene stability in a hydrocarbon-containing material having solvated asphaltenes therein is disclosed. The method involves the steps of: (a) precipitating an amount of the asphaltenes from a liquid sample of the hydrocarbon-containing material with an alkane mobile phase solvent in a column; (b) dissolving a first amount and a second amount of the precipitated asphaltenes by gradually and continuously changing the alkane mobile phase solvent to a final mobile phase solvent having a solubility parameter at least 1 MPa0.5 higher than the alkane mobile phase solvent; (c) monitoring the concentration of eluted fractions from the column; (d) creating a solubility profile of the dissolved asphaltenes in the hydrocarbon-containing material; and (e) determining one or more asphaltene stability parameters of the hydrocarbon-containing material.

Abstract: A new fractionation device shows desirable features for exploratory screening and biomarker discovery. The constituent MSCs may be tailored for desired pore sizes and surface properties and for the sequestration and enrichment of extremely low abundant protein and peptides in desired ranges of the mass/charge spectrum. The MSCs are effective in yielding reproducible extracts from complex biological samples as small as 10 ?l in a time as short as 30 minutes. They are inexpensive to manufacture, and allow for scaled up production to attain the simultaneous processing of a large number of samples. The MSCs are multiplexed, label-free diagnostic tools with the potential of biological recognition moiety modification for enhanced specificity. The MSCs may store, protect and stabilize biological fluids, enabling the simplified and cost-effective collection and transportation of clinical samples.

Abstract: The present invention pertains to a method of ion chromatography wherein a specialized electrodeionization (EDI) apparatus is used for (1) the preparation of a pure acid or pure base for use as an eluent in a chromatographic separation column and/or (2) for ion suppression (or neutralization) of the acid or base after it has been used to elute ions from a chromatographic separation column. Methods for trace ion removal, acid and base neutralization, and ion suppression using a specialized EDI apparatus are also described. The methods described herein allow for the ion suppression of samples containing chloride, nitrate, and other electrochemically active anions, without causing damage to the suppressor.

Abstract: The invention provides an oil-based ink composition for ink jet recording, which is best suited for printing onto polyvinyl chloride substrates, and improved in terms of both the stabilities of ink on storage and jetting, and a method for inspecting the same. The oil-based ink composition for ink jet recording comprises as a main solvent at least a specific polyalkylene glycol dialkyl ether and containing at least a specific quinacridone pigment component. That composition should be such that when a measuring sample comprising it is analyzed by means of high speed liquid chromatography under specific measuring conditions, the concentration of a component having a peak at the holding time of 10.3 minutes is 0.4 ppm or less.

Abstract: A liquid gas vaporization and measurement system, and associated method, for efficiently vaporizing a continuous sample of liquid gas, such as liquid natural gas (LNG), and accurately determining the constituent components of the gas. A constant flow of liquid gas sampled from a mass storage device is maintained in a vaporizing device. Within the vaporizing device the liquid gas is flash vaporized within heated narrow tubing. The liquid gas is converted to vapor very quickly as it enters one or more independently operating vaporizer stages within the vaporizing device. The vapor gas is provided to a measuring instrument such as a chromatograph and the individual constituent components and the BTU value of the gas are determined to an accuracy of within +/?0.5 mole percent and 1 BTU, respectively.

Abstract: A method of liquid chromatography includes providing an injection valve, drawing a sample and a diluent while mixing, pushing the mixed sample and diluent onto a sample loop of the injection valve, and injecting the mixed sample and diluent. An analytical apparatus includes a proportioning unit, an injection valve having a sample loop, and a sample pump. The injection valve has a draw state and a load state, and has a port in fluidic communication with an outlet port of the proportioning unit. The sample pump is in fluidic communication with the outlet port of the proportioning unit, if the injection valve is in the draw state, to draw both a sample and a diluent through the proportioning unit and the injection valve, and in fluidic communication with the sample loop, if the injection valve is in the load state, to push the drawn sample and diluent onto the sample loop.

Abstract: The present invention relates to a method and apparatus for chromatographically analyzing each of a plurality of samples in detector, comprising an autosampler to contain a plurality of samples for chromatographic analysis and a pluraltity of chromatographic systems, each system comprising one or more pumps and one or more chromatographic columns. A detector is included for detecting compounds in the samples from each of the chromatography systems along with a valve positioned between the detector and the plurality of chromatography systems, the valve permitting each sample to reach the detector in sequence. A computer control device is included which adjusts the introduction of samples from the autosampler into the plurality of chromatography systems as well as the position of the valve to sequentially separate and deliver compounds within the samples to the detector.

Abstract: The invention pertains to a sample injector for liquid chromatography, particularly for high performance liquid chromatography, with a controllable injection valve (3) that features at least one waste port (12) for discharging fluid under low pressure, two sample loop ports (13, 16) and two high-pressure ports (14, 15) for supplying and discharging fluid under high pressure, wherein one high-pressure port (15) can be connected to a pump (40) and the other high-pressure port (14) can be connected to a chromatography column (41), with a sample loop (44, 51, 52) that comprises a first sample loop section (51) that is connected to one of the sample loop ports (16) on one end and to a pump volume (V) of a sample conveying device (5) on the other end, as well as a second sample loop section (44, 52) that is connected to the other sample loop port (13) on one end and to the pump volume (V) of the sample conveying device (5) on the other end.

Abstract: A sample injector includes a rotary valve element with a straight passageway through it, which element may be rotated to connect a port communicating with a source of solvent with a port communicating with an inlet to a column. Another passageway connects two adjacent ports. The valve element may be rotated to one position in which it provides a straight path for sample to be injected, a second position in which it provides a path that connects a source of purge gas to the chromatographic system and a third position in which it provides a path that connects solvent to the column for preconditioning.

Abstract: A transfer structure for droplet-based microfluidic analysis is characterized by a first conduit containing a first stream having at least one immiscible droplet of aqueous material and a second conduit containing a second stream comprising an aqueous fluid. The interface between the first conduit and the second conduit can define a plurality of apertures, wherein the apertures are sized to prevent exchange of the first and second streams between conduits while allowing lossless transfer of droplets from the first conduit to the second conduit through contact between the first and second streams.

Abstract: The present invention is directed to a fast, nondestructive measurement method for determining the contents of solid, liquid and/or suspended flowing organic compounds. The arrangement according to the invention comprises a sample vessel, a pump, and a measurement cell which form a unit together with a spectroscopic measurement head. The measurement cell is connected to the pump, which can be regulated to vary the flow rate, and to the sample vessel by a pipe, and the spectroscopic measurement head and the regulatable pump have electrical connections to a controlling and evaluating unit. Due to its compact construction, the solution which makes use of the principle of transflection is also particularly suited to mobile use, for example, to determine the components of liquid manure while the latter is being dispensed. In principle, the solution can be transferred to any applications with suspensions or pumpable, homogeneous and inhomogeneous materials.

Abstract: A liquid chromatography/mass spectrometry system includes a chromatographic column through which an effluent passes, wherein the effluent comprises a plurality of analytes that correspond to a plurality of chromatographic peaks and an eluent; a post-column splitter having at least two output ports through which the effluent of the column is split to at least a first portion and a second portion; a mass spectrometer configured to receive the first portion from a first of the output ports for analysis; and a tube connected to a second of the output ports configured to prevent substantial evaporation of the eluent in the second portion until undergoing mass spectrometry. The second portion has a plurality of separated analytes corresponding to at least two chromatographic peaks. A method of using the system is also disclosed.

Abstract: Apparatus and methods related to centrifugal liquid chromatography are described. An angular velocity can be simultaneously imparted to a large number of chromatographic enclosures. Via centrifugal forces, a mobile phase fluid including a sample can be driven through a stationary phase within the chromatographic enclosure to perform a chromatographic separation process on components of the sample. The use of centrifugation as a driving force can allow significantly smaller stationary phase particles to be employed as compared to high performance liquid chromatography (HPLC). Further, for an equivalent chromatographic separation process, the use of centrifugation can provide much greater separation efficiencies than HPLC.

Abstract: Disclosed is an improved post-column fluorimetric determination-boric acid complex anion exchange method which allows analysis of mannose-6-phosphate. The disclosed method is a method for separation analysis of reducing sugars using column chromatography, comprising loading a sample onto an anion exchange column, washing the column by allowing to flow a sufficient volume of a first mobile phase consisting of an aqueous solution of a predetermined concentration of boric acid containing a predetermined concentration of a water-soluble inorganic salt through the column, supplying a second mobile phase with an elevated concentration of the salt to elute the reducing sugars, adding to the eluate a basic amino acid, heating, and continuously measuring and recording the intensity of fluorescent light emitted under irradiation with excitation light.

Abstract: A fluidic device comprising a capillary for conducting a fluid and a pivot arm configured for being pivoted, wherein the pivot arm supports at least a portion of the capillary, and a part of the capillary is coiled to at least partially compensate stress resulting from pivoting of the pivot arm.

Abstract: An optically active N-(?-mercaptopropionyl)glycine, i.e., R-(?)-N-(?-mercaptopropionyl)glycine or S-(?)-N-(?-mercaptopropionyl)glycine, a preparation method thereof, a pharmaceutical preparation containing the compound or a pharmaceutically acceptable salt or ester thereof, and use of the same in preparation of detoxification medicament for improving metabolism, are provided.

Abstract: An acid or base is generated in an aqueous solution by the steps of: (a) providing a source of first ions adjacent an aqueous liquid in a first acid or base generation zone, separated by a first barrier (e.g., anion exchange membrane) substantially preventing liquid flow and transporting ions only of the same charge as said first ions, (b) providing a source of second ions of opposite charge adjacent an aqueous liquid in a second acid or base generation zone, separated by a second barrier transporting ions only of the same charge as the second ions, and (c) transporting ions across the first barrier by applying an electrical potential through said first and second zones to generate an acid-containing aqueous solution in one of said first or second zones and a base-containing aqueous solution in the other one which may be combined to form a salt. Also, electrolytic apparatus for performing the above method.

Abstract: A specimen pretreating device which includes a micro scale or nano scale high-performance liquid chromatograph having a capillary column (2), a probe (1) integrally formed at the tip of the capillary column (2), and an additive feeding flow passage (16). The probe (1) is formed in a multi-tube structure in which a plurality of tubes are disposed on the same axis. The innermost tube is located at the tip of the capillary column (2), and one of the outer tubes is used as an additive supply tube converging an additive solution to an eluent solvent eluted from the capillary column (2) to form liquid drops including the eluent solvent and an additive, and the liquid drops are released from the tip of the probe (1). The specimen is not diffused since a detector is not present in the flow passage.

Abstract: A system is provided to calibrate for a near infrared analytical testing device configured to screen for ethanol production yield of an ethanol-producing material. The system comprises a first analytical testing device configured collect a first and second signal associated with a characteristic parameter of a sample material, wherein the characteristic parameter is capable of enabling evaluation of ethanol production yield for plant material corresponding to the sample material. A drying unit is configured to drive the sample material to a substantially standard moisture level. A fermentation unit is configured to ferment the sample material. A second analytical testing device is configured to collect a reference measurement associated with the characteristic parameter. The reference measurement is capable of being correlated to the first and second signals for building a calibration model used to evaluate ethanol production yield of plant material corresponding in composition to the sample material.

Abstract: A multi-dimensional chromatographic method for the separation of N-glycans. The method comprises providing a glycan preparation that includes at least one negatively charged N-glycan. The glycan preparation is then separated by anion-exchange chromatography and at least one secondary chromatographic technique.

Abstract: The present invention relates to a device and method for separation of proteins and other biomolecules. A preferred use is for sample preparation of crude as well as pre-fractionated samples. In a preferred embodiment, the device of the present invention is a pipette tip having dual channels, one for inlet of sample and one for outlet. The outlet, but not the inlet, channel is provided with sample separation media for separation of a desired biomolecule from a sample. The flow through the sample separation media is unidirectional.

Abstract: The present invention provides a detector for a chromatograph capable of obtaining the accurate retention time, even if there is a frequency deviation of the clock signal for determining the timing of data sampling, by conforming the progress of the detector internal time accompanied with the repetition of the sampling to that of real time. In the detector, consecutive data sampling intervals are defined to be a time adjustment period, and in one time adjustment period, each of the sampling intervals whose number is given as the interpolation value M is elongated by a predetermined number of clock counts compared to each of the other sampling intervals. If the clock signal has a positive frequency deviation, the apparatus internal time determined by the clock signal progresses faster than real time.

Abstract: An elongated flow-through degassing apparatus includes an elongated gas and liquid impermeable outer member and a gas-permeable, liquid-impermeable inner barrier extending within the outer member and at least partially along a first chamber defined within the outer member. The apparatus also includes inlet and outlet connection structures operably coupled to respective portions of the outer member and a second chamber defined by the inner barrier to further enable a sealed engagement between the outer member and the inner barrier, and to provide for connection devices to operably couple the degassing apparatus of the present invention to respective spaced apart components. The degassing apparatus may be sufficiently flexible so as to be readily manipulatable into desired configurations.

Abstract: Device for recovering a material to be measured comprising a reservoir filled with a sample holding material impregnated with a sample liquid in which a material to be measured is dissolved and an adsorbing column for adsorbing the material to be measured, wherein the reservoir and the adsorbing column are communicated by a straight pipe, and further communicated with a recovery vessel via a recovery pipe branched out the straight pipe. Accordingly, operations of opening/closing or switching a first valve provided on the in-flow side of the reservoir, a second valve provided on the out-flow side of the adsorbing column, and a third valve provided to a vent hole to the atmosphere in the recovery vessel, make it possible to recover the material to be measured without depositing the material on the valves.

Abstract: A pumping apparatus for a high performance liquid chromatography system (350) is disclosed. The pumping apparatus comprises a piston (1) for reciprocation in a pump working chamber (3) to compress liquid in the pump working chamber (3) to a high pressure at which compressibility of the liquid becomes noticeable. At least one of the piston (1) and the pump working chamber (3) is at least partially coated with or comprised of silicon carbide.

Abstract: Provided are methods for determining the amount of vitamin C in a sample using mass spectrometry. The methods generally involve ionizing vitamin C in a sample and detecting and quantifying the amount of the ion to determine the amount of vitamin C in the sample.

Abstract: The present invention relates to a method and apparatus for chromatographically analyzing/purifying samples in a detector, comprising an autosampler to contain a sample for chromatographic analysis and a chromatographic system comprising a loading pump, and an eluting pump and at least one chromatography column. A detector is included for detecting compounds in the sample from the chromatography system. The loading pump establishes a flow of a loading solvent through the autosampler to dissolve the sample and to load the sample on the chromatography column. The eluting pump establishes a flow of eluent of increasing strength through the column and to the detector. In addition, the present invention provides improved methods of screening and preparative chromatography.

Abstract: A capture and purification apparatus is configured as a stand-alone apparatus or as part of a larger system. The capture and purification apparatus can be configured as a microfluidic cartridge that includes microfluidic circuitry and individually controlled valves. The microfluidic cartridge can be configured to function independently, or can be configured to be coupled to a separate instrument that provides the actuation to perform the capture and purification process. The capture and purification apparatus is configured as a volume-driven system that applies single-direction valves, a single fluid driving device, and fluid lines to control and discretely direct fluid flow within a full-loaded fluidic system. Such control enables various fluid sample processing techniques to be performed including, but not limited to, lysis, thermal cycling, and/or target analyte capture and purification, for example using a combination of ion-exchange chromatography and size-exclusion chromatography (SEC).

Abstract: Embodiments of the present invention are directed to methods and apparatus for placing a sample in a chromatographic system. The device and method feature placing samples held in a sample loop to pressurization prior to placing such sample loop in communication with high pressure conduits.

Abstract: A device for collecting a sample and for depositing the sample in a sample-receiving port of a measuring apparatus comprises a first connection element (8) with three connections for fluid, and a second connection element (7), and a hollow needle (5) for collecting and depositing the sample. A first feed line (9) for a first washing liquid is connected via a first pump to a first connection for fluid of the first connection element (8). A second feed line (10) for a second washing liquid is connected via a second pump to a second connection for fluid of the first connection element (8). A connection for fluid of the second connection element (7) is connected to the third connection for fluid of the first connection element (8), and the second connection element (7) is connected to a dosing device (1) and to the hollow needle (5). A valve (2) is arranged between the third connection for fluid of the first connection element (8) and the second connection element (7).

Abstract: A temperature control unit (216) for a column (201) of a fluidic device (200) for analyzing a fluidic sample, wherein the fluidic device (200) is adapted to conduct the fluidic sample through the column (201), the temperature control unit (216) being arranged to adjust a temperature of the fluidic sample in a flow path between an inlet 5 (207) of the column (201) and an outlet (208) of the column (201) so that a temperature adjustment effect occurs selectively in an interior of the column (201).

Abstract: A capillary column, and method for forming a capillary column, in which the capillary column comprises at least one porous segment at a terminus of the capillary column, wherein the at least one porous segment is formed by exposing the segment to one or more of a solution of acid, base, and a mechanical tool.

Abstract: The preparation of atorvastatin calcium epoxide dihydroxy (AED) is described. AED can be used as a standard or marker in determining the amount of AED in a sample. AED can therefore be used as a tool in preparing atorvastatin calcium substantially free of AED.

Abstract: Disclosed herein is a liquid chromatograph in which a fractionation flow path, a non-fractionation flow path, a concentration flow path, and a secondary analysis flow path can be provided by operating flow path switching valves and flow path selecting valves. The concentration flow path and either one of the fractionation flow path and the non-fractionation flow path can be provided at the same time, and the secondary analysis flow path and either one of the fractionation flow path and the non-fractiontation flow path can also be provided at the same time.

Abstract: The present invention is directed to a SPE-LC-MS-MS method for quantitatively determining risedronate in a urine sample.

Abstract: A technique for extracting an objective component from a sample solution and obtaining the component in the form of equally separated powder aliquots is provided. A peak detector 12 detects the start point of the peak of the objective component on a chromatogram created from detection signals produced by a detector 5. Then, a controller 14 immediately changes a valve 6 so that the eluate will flow to a trap column 7a. A peak area processor 13 calculates the peak area of the objective component in real time. When the calculated area has exceeded a threshold, the controller 14 changes the valve 6 so that the eluate will flow to the next trap column 7b. Thus, the trap columns 7a to 7d are sequentially selected to divide the peak into one or more portions with the same area so that the objective component in the eluate will be sequentially captured by the trap columns 7a to 7d.

Abstract: The present invention relates to impurities of donepezil, or a pharmaceutically acceptable salt thereof, which may be produced during synthesis and storage of donepezil, or a pharmaceutically-acceptable salt thereof, and to methods of identifying such impurities. The invention also relates to the use of these impurities in analytical techniques and to product that contain certain low levels of these impurities in particular the following impurities are disclosed: formula (I) and formula (II).

Abstract: The present invention provides apparatus and methods for detection of an analyte in liquid chromatography with increased detection sensitivity and signal resolution.

Abstract: Separating trihalomethanes from drinking water samples (via a process such as capillary membrane sampling, and the like) followed by gas chromatograph analysis to determine quantity measurements and species identification of such trihalomethane (THM4) contaminants therein is provided. With the necessity to chlorinate drinking water to remove harmful bacteria and other potential toxins, trihalomethane byproducts are generated that may harm humans after consumption as well due to suspect carcinogenicity of such compounds. A reliable manner of measuring such drinking water supplies for such THM4 contaminants at locations far from the source and closer to dispensers is highly desirable.

Abstract: This invention concerns an automatic sampler (AS) associable with two or more chromatographic analysis instruments (GC1, GC2, GCn), such as gas chromatographs for example, which can be interfaced with two or more separate data systems (DS1, DS2, DSn), each operating in an independent manner.

Abstract: This invention is a method and device for use with multi-dimensional chromatography that utilizes partial modulation. An analyte-bearing sample is subjected to a first dimension of chromatography. Thereafter the separated analyte-bearing sample is diluted with a modulated second carrier such at the analyte-bearing sample is not stopped or its temperature altered. The partially modulated analyte-bearing sample then feeds into a secondary column where the analyte-bearing sample is further separated.

Abstract: This invention provides a chromatographic apparatus comprising an introduction part for a carrier gas as a main mobile phase medium, a sample injection part, a separation column comprising a stationary phase formed by coating a polymeric material, a part for housing the separation column, and a detector. The sample injection part is provided between the carrier gas introduction part and the inlet of the separation column. The chromatographic apparatus further comprises a solvent introduction means for adding a liquid solvent as a second mobile phase medium to a carrier gas and mixing them together, a mechanism for regulating the amount of the carrier gas introduced and the amount of the solvent added, and a mechanism for regulating the temperature of each flow passage. A plurality of clogging liquid stoppers, of which the liquid film thickness is equal to the inner diameter of a capillary column, are partly provided at predetermined intervals within the column in a longitudinal direction of the column.

Abstract: A device (100) for detecting a condition in a fluid system (14) and initiating a response based on the condition. The device consists of a sensor (12) that responds to a first and second condition of the fluid (13) in the system and a control means (10) that receives the responses and generates command signals in based on the received response. In a liquid chromatography system, the device is used to determine the volume of components of the fluid system. In addition, the device allow leaks to be detected and permits the fluid in the system to be transported at a optimum speed. The device is well implemented utilizing to a light emitter and light receptor that are sensitive to the fluid in either a gaseous or liquid state.

Abstract: Methods and devices are provided for overcoming detrimental diffusive effects in a sample liquid stream by forming segmented liquid bodies (e.g., droplets) from a sample liquid stream in an immiscible liquid stream. The liquid bodies are formed at the intersection of a channel providing the sample liquid stream and a channel providing the immiscible liquid stream. The formed liquid bodies compartmentalize the portion of the sample liquid stream from which the liquid bodies are formed, thus minimizing the detrimental effects of diffusion that occur in a continuous liquid stream.

Abstract: The invention relates to a gas chromatography system for the direct coupling of liquid chromatography and gas chromatography or for the introduction of large sample volumes, which is configured to operate selectively in a retention mode. The inventive system includes a tube (2) having a first inlet part (21), a second inlet part (22) and a retention part (3) between said two inlet parts. The system also includes two gas delivery subsystems (6, 7) and a sample introduction conduit (101) which is connected to the first inlet part (21). The system further includes a discharge conduit (9) and a gas chromatography column (300), both of which are connected to the second inlet part (22). The invention also relates to a gas chromatography method.

Abstract: Markers are provided that are predictive of Alzheimer's disease. Levels of these markers, when different from a standard, are indicative of a patient being at risk of having or developing Alzheimer's disease.

Abstract: Disclosed is a lateral flow quantitative assay method capable of quantitatively determining the concentration and analyzing the spatial distribution of a disease marker by employing the principle of the laser-induced fluorescence detection technique, which is based on detecting emitted fluorescence when laser light is focused to the disease marker deposited onto a lateral flow quantitative assay chip. The present invention discloses a strip, a laser-induced epifluorescence detection device and a small scanner for the assay method.