Via Agrobacterium Patents (Class 800/294)
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Patent number: 8779238Abstract: The present invention provides methods for transforming Camelina plants. In particular, the present invention relates to transforming Camelina sativa plants through contacting the plants to a dipping solution comprising Agrobacterium, a sugar, and a nonionic surfactant. The methods do not require a vacuum filtration step. The present invention provides, for example, useful methods for developing transformation systems for Camelina sativa that can enable manipulation of its agronomic qualities.Type: GrantFiled: March 19, 2009Date of Patent: July 15, 2014Assignee: Global Clean Energy Holdings, Inc.Inventors: Thu Nguyen, Xunjia Liu, Jay Derocher
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Patent number: 8771703Abstract: The present invention provides a recombinant soluble trimeric hemagglutinin (rHA) protein comprising a hemagglutinin ectodomain and an oligomerization domain. The rHA is produced as a soluble homotrimer, and may further comprises a signal peptide and/or an endoplasmic reticulum (ER) retention signal. The invention is also directed to nucleic acids encoding the rHA of the invention, as well as vectors and chimeric constructs comprising the nucleic acid. Methods of producing the rHA are also provided. The rHA described herein may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.Type: GrantFiled: July 7, 2009Date of Patent: July 8, 2014Assignee: Medicago Inc.Inventors: Manon Couture, Nathalie Landry, Louis-Philippe Vezina, Michéle Dargis
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METHODS FOR TRANSFORMING TARWI AND FOR PRODUCING MOLECULAR FARMING PRODUCTS IN TRANSGENIC TARWI SEED
Publication number: 20140186924Abstract: The present disclosure describes reproducible methods for the Agrobacterium-mediated production of stable, genetically transformed, fertile tarwi plants (Lupinus mutabilis Sweet) having seed-specific expression of human adenosine deaminase enzyme (hADA) or a functional variant thereof. The method involves slicing a tarwi seed embryo to produce an explant; infecting the explant in co-cultivation medium containing an agrobacterium having a polynucleotide sequence encoding hADA or variant; thereby generating a transformed explant; elongating a transformed shoot from the transformed explant; and regenerating a transformed tarwi plant from the elongated shoot. Seeds and plants so formed are also described herein. Further, methods for the recovery and purification of recombinant hADA, or functional variant from a transformed tarwi plant are described.Type: ApplicationFiled: October 17, 2013Publication date: July 3, 2014Applicants: National Research Council of Canada, PRAIRIE PLANT SYSTEMS INC.Inventors: Ketan M. DOSHI, Natalia N. LOUKANINA, Brent POLLOCK, Patricia POLOWICK, Larry HOLBROOK -
Patent number: 8759609Abstract: The present invention relates to a novel transformation system for generating transformed corn plants. In particular, the invention relates to a rapid selection system at an elevated temperature that allows faster and more efficient transformation.Type: GrantFiled: March 22, 2010Date of Patent: June 24, 2014Assignee: Monsanto Technology LLCInventors: Tishu Cai, Shubha Subbarao, Yuechun Wan, Richard A. Yingling, Michael T. Mann, Anita Otal, David D. Songstad
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Patent number: 8759616Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.Type: GrantFiled: March 19, 2013Date of Patent: June 24, 2014Assignee: Monsanto Technology LLCInventors: Larry A. Gilbertson, Xudong Ye
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Publication number: 20140173780Abstract: A method is disclosed for the Agrobacterium-mediated germline transformation of soybean, comprising infecting split soybean seeds, with a portion of the embryonic axis, with Agrobacterium tumefaciens containing a transgene. The method can further comprise regenerating the explants produced from the transformation of the split soybean seeds comprising a portion of embryonic axis in vitro on selection medium.Type: ApplicationFiled: December 18, 2013Publication date: June 19, 2014Inventors: Dayakar Pareddy, Sivarama R. Chennareddy, Tatyana Minnicks, Olga Karpova, David Griffin, Jayakumar P. Samuel, Kelley A. Smith, Rodrigo Sarria-Millan, Tejinder Kumar Mall
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Patent number: 8748698Abstract: A genome shuffling method for autogamous plants, including producing individuals having the following three traits in a tight coupling linkage by a gene engineering technique selected from a transgenic technique and a gene targeting technique: 1) dominant male sterility, 2) chemical tolerance and 3) lethality inducible by activating an inducible promoter, selecting, from progeny of the individuals, male-sterile individuals by means of the chemical tolerance described in 2) and male-fertile individuals by means of the lethality described in 3), arranging the male-sterile individuals and the male-fertile individuals close together in flowering periods thereof, so that the male-sterile individuals are crossed with the male-fertile individuals, harvesting seeds from the male-sterile individuals, and repeating outcrossing using the seeds from generation to generation.Type: GrantFiled: December 10, 2013Date of Patent: June 10, 2014Inventor: Junichi Tanaka
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Patent number: 8742202Abstract: Methods for the transformation of sugar cane are provided. The methods comprise utilizing sugar cane immature shoots as the source of plant material for transformation. Segments of the immature shoot are excised and transformed by any suitable transformation methodology. In some embodiments, the segments are cultured in embryogenic culture induction medium prior to transformation. Transformation can be performed via Agrobacterium-mediated gene delivery, biolistic transformation, and the like. Transgenic plants are regenerated from plantlets grown under conditions favoring growth of transformed cells while substantially inhibiting growth of non-transformed cells.Type: GrantFiled: December 1, 2009Date of Patent: June 3, 2014Assignee: Syngenta Participations AGInventor: Heng Zhong
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Patent number: 8710207Abstract: The present disclosure relates, in some embodiments, to compositions, organisms, systems, and methods for expressing a gene product in a plant using a expression control sequence (ECS) operable in monocots and/or dicots. For example, (i) an isolated nucleic acid may comprise an ECS (e.g., a sugarcane bacilliform virus promoter) and, optionally, an exogenous nucleic acid (ExNA) operably linked to the ECS; (ii) an expression vector may comprise an ECS; an ExNA; and, optionally, a 3? termination sequence, wherein the ECS has promoter activity sufficient to express the ExNA in at least one monocot and at least one dicot; (iii) a microorganism, plant cell, or plant may comprise an isolated nucleic acid; (iv) a method for constitutively expressing an ExNA in a plant (e.g.Type: GrantFiled: May 10, 2011Date of Patent: April 29, 2014Assignee: The Texas A&M University SystemsInventors: T. Erik Mirkov, Jong Won Park, San-Ji Gao
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Publication number: 20140109260Abstract: Methods of providing gene suppression DNA in a eukaryotic organism comprising introducing a first DNA segment and at least one second DNA segment into the genome of the organism. One of the DNA segments contains a promoter and a transcribable DNA. Another DNA segment contains at least part of the transcribable DNA. When inserted in tandem, the DNA segments are assembled in vivo forming a recombinant transcription unit. RNA transcribed from the transcription unit can form double-stranded RNA.Type: ApplicationFiled: December 17, 2013Publication date: April 17, 2014Applicant: MONSANTO TECHNOLOGY LLCInventors: JoAnne Fillatti, Larry Gilbertson, Toni Voelker
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Publication number: 20140096285Abstract: A transgenic soybean plant harboring an exogenous nucleic acid molecule encoding porcine reproductive and respiratory syndrome virus ORF7 immunogen and methods for using the same to induce an antibody response and prevent a porcine reproductive and respiratory syndrome virus infection are provided.Type: ApplicationFiled: October 2, 2013Publication date: April 3, 2014Applicant: The Board of Trustees of the University of IllinoisInventor: Schuyler S. Korban
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Patent number: 8686223Abstract: The present invention relates to methods for the regeneration and Agrobacterium-mediated transformation of plants in the genera of Jatropha, more specifically, in Jatropha curcas.Type: GrantFiled: July 3, 2012Date of Patent: April 1, 2014Assignee: Joil (S) Pte Ltd.Inventors: Hui Zhu Mao, Jian Ye, Nam Hai Chua
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Patent number: 8674178Abstract: Described herein are methods useful for producing proteins, such as enzymes, by agrofiltration. The methods involve producing an Agrobacterium with a Ti plasmid encoding a cellulase, infecting plant cells with the Agrobacterium, allowing expression of the cellulase, and recovering the cellulase from the plant cells. In one embodiment, the protein produced is an endoglucanase.Type: GrantFiled: August 19, 2009Date of Patent: March 18, 2014Assignee: The Regents of the University of CaliforniaInventors: Karen A. McDonald, Benjamin E. Lindenmuth, Abhaya M. Dandekar, Bryce W. Falk
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Patent number: 8674176Abstract: The present invention relates to enzymes involved in lipid metabolism. In particular, the present invention provides coding sequences for Arabidopsis Desaturases (ADS), the encoded ADS polypeptides, and methods for using the sequences and encoded polypeptides, where such methods include decreasing and increasing saturated fatty acid content in plant seed oils.Type: GrantFiled: January 13, 2010Date of Patent: March 18, 2014Inventors: Ingo H. Heilmann, John Shanklin
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Patent number: 8669417Abstract: The invention provides methods and compositions for increasing the efficiency of genetic transformation of host cells, including plant cells, and other eukaryotic cells, by reducing the expression of a polypeptide active in a pathway, such as the NHEJ pathway, for repairing damage to the cellular genome. In certain embodiments, the polypeptide is active in repairing double strand breaks (DSB's) of a cellular genome, and may include XRCC4, KU70, KU80, the DNA-activated Protein Kinase (DNA-Pkcs), and ATM. Methods for enhancing the resistance of plant cells to Crown Gall disease are also provided. In another aspect, genetic regulatory elements are provided, including an XRCC4 promoter.Type: GrantFiled: July 20, 2010Date of Patent: March 11, 2014Assignee: The Samuel Roberts Noble Foundation, Inc.Inventors: Zarir E. Vaghchhipawala, Kirankumar Mysore
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Publication number: 20140068818Abstract: The present invention relates to a method for producing transgenic plants comprising Agrobacterium mediated in planta transformation of seeds capable of overexpressing the RNAi suppressor proteins. The invention also relates to a rapid method for generating transgenic rice plants stably expressing the viral suppressors of RNAi by directly transforming the seeds and growing them in presence of appropriate selection markers. The invention provides an effective and efficient method for Agrobacterium mediated in planta transformation of rice seeds.Type: ApplicationFiled: August 30, 2013Publication date: March 6, 2014Applicant: INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGYInventors: Neeti Sanan MISHRA, Sudhanshu Sekhar DAS
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Publication number: 20140059718Abstract: The present invention provides a binary vector containing only the elements essential for maintenance in E. coli and Agrobacterium and transforming plant cells, for single copy insertions in transgenic plants with little or no vector backbone integrations. The vectors of the invention are useful for stable or transient expression of one or more genes of interest.Type: ApplicationFiled: January 17, 2012Publication date: February 27, 2014Applicant: PHILIP MORRIS PRODUCTS S.A.Inventor: Prisca Campanoni
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Publication number: 20140059717Abstract: A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.Type: ApplicationFiled: July 18, 2013Publication date: February 27, 2014Applicant: Monsanto Technology LLCInventors: Anisha Akula, David R. Duncan, Brenda A. Lowe, Michael T. Mann, William L. Petersen, Jyoti R. Rout, David D. Songstad, Joel B. Wilks, Wanggen Zhang
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Patent number: 8642839Abstract: Compositions and methods for transiently expressing proteins in a plant are provided. The compositions comprise plants, seeds, plant tissues, and plant parts expressing a protein, wherein the protein is expressed transiently and the transient expression of the protein can be used as a predictive model of how said protein will be expressed in stable transgenic plants in regards to qualitative and quantitative data. The predictive model may be used but is not limited to: promoter evaluation, evaluation of expression cassette construction for best performance (e.g. addition of enhancers or gene silencing suppressors), evaluation of best ways to express heterologous genes (e.g. point mutations, targeting), fast evaluation of endogenous gene knockout, evaluation of protein expression levels, cellular targeting, tissue targeting, transcriptional enhancers, translational enhancer protein toxicity and metabolic profiling. Further provided are methods of use.Type: GrantFiled: June 11, 2010Date of Patent: February 4, 2014Assignee: Syngenta Participations AGInventor: Kasimalai Azhakanandam
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Patent number: 8629322Abstract: A genome shuffling method for autogamous plants, including producing individuals having the following three traits in a tight coupling linkage by a gene engineering technique selected from a transgenic technique and a gene targeting technique: 1) dominant male sterility, 2) chemical tolerance and 3) lethality inducible by activating an inducible promoter, selecting, from progeny of the individuals, male-sterile individuals by means of the chemical tolerance described in 2) and male-fertile individuals by means of the lethality described in 3), arranging the male-sterile individuals and the male-fertile individuals close together in flowering periods thereof, so that the male-sterile individuals are crossed with the male-fertile individuals, harvesting seeds from the male-sterile individuals, and repeating outcrossing using the seeds from generation to generation.Type: GrantFiled: October 29, 2010Date of Patent: January 14, 2014Inventor: Junichi Tanaka
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Publication number: 20140007300Abstract: A method for increasing efficiency of germplasm screening for transformability may include providing a plurality of lines of plant target tissue to be transformed, characterizing each of the lines to provide characterization data, the characterization data comprises DNA or nucleic acid delivery technique response data and tissue culture response data, eliminating one or more of the plurality of lines based on the characterization data without performing transformation of the plurality of lines, such that a subset of the plurality of lines remains, and performing transformation experiments on the subset of the plurality of lines. The method may also include selecting a DNA or nucleic acid delivery technique protocol and a tissue culture protocol prior to the characterization.Type: ApplicationFiled: February 1, 2013Publication date: January 2, 2014Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: MYOENG-JE CHO, XINLI E. WU, DEPING XU, ZUO-YU ZHAO
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Patent number: 8618375Abstract: A novel maize variety designated X13B645 and seed, plants and plant parts thereof, produced by crossing Pioneer Hi-Bred International, Inc. proprietary inbred maize varieties. Methods for producing a maize plant that comprises crossing hybrid maize variety X13B645 with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X13B645 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. This invention relates to the maize variety X13B645, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X13B645. This invention further relates to methods for producing maize varieties derived from maize variety X13B645.Type: GrantFiled: March 22, 2011Date of Patent: December 31, 2013Assignee: Pioneer Hi Bred International IncInventors: Mark David Hoffbeck, Russell L. Fox, Luis A. Verde Chifflet
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Publication number: 20130347144Abstract: The present disclosure relates to a plant promoter and a method of transforming Oxalis tuberosa. In detail the present disclosure relates to a plant promoter, a vector, including the promoter, a method of producing target protein using the vector, target protein produced by the method, a method for producing a transformed cell and a plant using the vector and a propagule of the plant.Type: ApplicationFiled: March 6, 2013Publication date: December 26, 2013Inventors: Ketan DOSHI, Natalia LOUKANINA
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Patent number: 8614366Abstract: In various embodiments, methods described herein comprise the use of water-soluble cationic fullerene derivatives for improving plant genetic transformation. Cationic Fullerene derivatives of the invention possess DNA binding and compaction activity and provide a new method to deliver DNA into plant cells for plant transformation. Water-soluble fullerene derivatives of the invention with anionic or non-polar substituents possess antioxidant (free radical scavenging) activity, provide improved yields and efficiency of plant transformation methods such as biolistic, Agrobacterium tumefaciens, or electroporation methods by limiting cellular damage and resulting cell death leading to higher yields of viable transformed cells in the process.Type: GrantFiled: September 7, 2010Date of Patent: December 24, 2013Inventor: Stephen R. Wilson
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Patent number: 8609934Abstract: Methods of providing gene suppression DNA in a eukaryotic organism comprising introducing a first DNA segment and at least one second DNA segment into the genome of the organism. One of the DNA segments contains a promoter and a transcribable DNA. Another DNA segment contains at least part of the transcribable DNA. When inserted in tandem, the DNA segments are assembled in vivo forming a recombinant transcription unit. RNA transcribed from the transcription unit can form double-stranded RNA.Type: GrantFiled: February 11, 2005Date of Patent: December 17, 2013Assignee: Monsanto Technology LLCInventors: Joanne Fillatti, Larry Gilbertson, Toni Voelker
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Patent number: 8604276Abstract: The subject application provides polynucleotides, compositions thereof and methods for regulating gene expression in a plant. Polynucleotides disclosed herein comprise novel sequences for a promoter isolated from Panicum virgatum (switchgrass) that initiates transcription of an operably linked nucleotide sequence. Thus, various embodiments of the invention comprise the nucleotide sequence of SEQ ID NO: 2 or fragments thereof comprising nucleotides 1 to 692 of SEQ ID NO: 2 that are capable of driving the expression of an operably linked nucleic acid sequence.Type: GrantFiled: June 9, 2010Date of Patent: December 10, 2013Assignee: University of Tennessee Research FoundationInventors: C. Neal Stewart, David George James Mann
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Patent number: 8581035Abstract: The invention provides methods for identifying regenerated transformed plants and differentiated transformed plant parts, obtained without subjecting plant cells to selective conditions prior to regenerating the cells to obtain differentiated tissues. In particular embodiments, the plant cells are corn plant cells. Methods for growing and handling plants, including identifying plants that demonstrate specific traits of interest are also provided.Type: GrantFiled: August 31, 2007Date of Patent: November 12, 2013Assignee: Monsanto Technology LLCInventor: Jyoti R. Rout
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Patent number: 8581045Abstract: This disclosure relates to a mutant Agrobacterium tumefaciens that is functionally deleted for the atu1060 gene that codes for the cyclic di-GMP synthase Atu1060, as well as methods for its use in transforming plants with desired transgenes. Such bacteria are more virulent than currently used strains of A. tumefaciens, and thus can be used to transform a wider variety of plants, such as plants that are traditionally recalcitrant to such transformation.Type: GrantFiled: November 19, 2010Date of Patent: November 12, 2013Assignee: The Board of Trustees of the University of IllinoisInventors: Stephen K. Farrand, David Michael Barnhart
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Patent number: 8581044Abstract: The present invention relates to the field of plant genetic engineering. More specifically, the present invention relates to seed specific gene expression during a defined period of embryogenesis. The present invention provides promoters capable of transcribing heterologous nucleic acid sequences in seeds, and methods of modifying, producing, and using the same.Type: GrantFiled: April 4, 2012Date of Patent: November 12, 2013Assignee: Monsanto Technology LLCInventors: Qi Wang, Lisa M. Weaver, Tim N. Oulmassov, Jeffrey Ahrens, Patrice Dubois, Jeffery Q. Shen
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Patent number: 8575427Abstract: The nucleotide sequence of a 992 bp region of cDNA and the nucleotide sequence of a 1973 bp (or a 1913 bp) of genomic DNA of the Gr-cm-1 gene were determined for G. rostochiensis. PCR primers and probes specific for G. rostochiensis and G. pallida were generated. PCR assays, including a real-time TaqMan PCR were used to identify G. rostochiensis and G. pallida and to differentiate G. rostochiensis from G. pallida. Transgenic hairy roots expressing Gr-cm-1 dsRNA were generated. There was a 52% reduction in the average number of females per root in the Gr-cm-1 dsRNA transgenic lines when compared with the infected control lines.Type: GrantFiled: July 24, 2009Date of Patent: November 5, 2013Assignee: The United States of America as represented by the Secretary of AgricultureInventors: Xiaohong Wang, Shunwen Lu
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Patent number: 8569582Abstract: The invention provides methods for transforming grass plants with Agrobacterium. The invention allows creation of transgenic grass plants without the need for callus as a target tissue for transformation, thus providing a rapid method for the production of transgenic grass plants. Transgenic grass plants produced by this method are also provided.Type: GrantFiled: June 22, 2010Date of Patent: October 29, 2013Assignee: The Samuel Roberts Noble FoundationInventors: Zeng-Yu Wang, Rangaraj Nandakumar
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Patent number: 8541653Abstract: The present invention relates to improved methods for the incorporation of DNA into the genome of a soybean (Glycine max) plant utilizing meristematic cells of primary or higher leaf nodes as target tissue by means of Agrobacterium-mediated transformation and subsequent regeneration of the transformed cells into a whole plant.Type: GrantFiled: June 4, 2005Date of Patent: September 24, 2013Assignee: BASF Plant Science GmbHInventors: Paula Olhoft, Leslie Grist, Libby Bernal, Sara Price, Diana Arias, Haiping Hong
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Patent number: 8536407Abstract: The subject invention concerns materials and methods for providing plants or plant tissue with increased resistance to heat conditions and/or increased starch biosynthesis. Increased resistance of a plant or plant tissue to heat conditions provides for decreased yield losses as compared to the yield losses generally observed at elevated temperatures. One aspect of the invention concerns polynucleotides that encode a mutant plant small subunit of AGPase. The subject invention also comprises a mutant plant small subunit of AGPase encoded by a polynucleotide of the invention. The subject invention also concerns plants comprising a polynucleotide of the invention and method for making the plants.Type: GrantFiled: March 26, 2009Date of Patent: September 17, 2013Assignee: University of Florida Research Foundation, Inc.Inventors: L. Curtis Hannah, Nikolaos Georgelis
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Publication number: 20130227746Abstract: A genetic screen for Arabidopsis mutants displaying a hyper-susceptible to Agrobacterium transformation (hat) phenotype was performed. The gene disrupted in the hat3 mutant encodes a putative myb-family transcription factor (MTF) that negatively regulates susceptibility to Agrobacterium-mediated transformation. Over-expression of an integrin-like protein results in plants that are hyper-susceptible to transformation. Manipulation of MTF, members of this protein family, and members of the integrin domain-like protein family for example At14a allows improved control of Agrobacterium transformation, including in crops.Type: ApplicationFiled: November 9, 2011Publication date: August 29, 2013Applicant: PURDUE RESEARCH FOUNDATIONInventors: Stanton B. Gelvin, Nagesh Sardesai
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Publication number: 20130227740Abstract: The present invention discloses transgenic plants having an altered level of NAP protein compared to that of a non-transgenic plant, where the transgenic plants display an altered leaf senescence phenotype relative to a non-transgenic plant, as well as mutant plants comprising an inactivated NAP gene, where mutant plants display a delayed leaf senescence phenotype compared to that of a non-mutant plant. The present invention also discloses methods for delaying leaf senescence in a plant, as well as methods of making a mutant plant having a decreased level of NAP protein compared to that of a non-mutant plant, where the mutant plant displays a delayed leaf senescence phenotype relative to a non-mutant plant. Methods for causing precocious leaf senescence or promoting leaf senescence in a plant are also disclosed. Also disclosed are methods of identifying a candidate plant suitable for breeding that displays a delayed leaf senescence and/or enhanced yield phenotype.Type: ApplicationFiled: February 28, 2013Publication date: August 29, 2013Applicant: CORNELL RESEARCH FOUNDATION, INC.Inventor: Cornell Research Foundation, Inc.
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Patent number: 8519227Abstract: There is provided an Agrobacterium transformation method for monocotyledons, comprising a step of infecting an intact seed.Type: GrantFiled: November 2, 2004Date of Patent: August 27, 2013Inventors: Hiroshi Tanaka, Toshiaki Kayano, Masashi Ugaki, Fumio Shiobara, Naoto Shibuya, Haruko Onodera, Kazuko Ono, Akemi Tagiri, Yaeko Nishizawa
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Publication number: 20130219564Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.Type: ApplicationFiled: March 19, 2013Publication date: August 22, 2013Applicant: Monsanto Technology LLCInventor: Monsanto Technology LLC
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Publication number: 20130212739Abstract: A process of transfecting a plant, comprising spraying parts of said plant with an aqueous suspension containing cells of an Agrobacterium strain and at least one abrasive suspended in said suspension, said Agrobacterium strain comprising a DNA molecule comprising a nucleic acid construct containing a DNA sequence of interest to be transfected into the plant.Type: ApplicationFiled: May 6, 2011Publication date: August 15, 2013Applicant: NOMAD BIOSCIENCE GMBHInventors: Anatoli Giritch, Yuri Symonenko, Simone Hahn, Doreen Tiede, Anton Shvarts, Patrick Roemer, Yuri Gleba
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Patent number: 8507758Abstract: Methods for identification of successful transformation without a need for in vitro selection are provided. Direct detection of nucleotide sequences of interest is described which eliminate the need for use of ancillary nucleotide sequences.Type: GrantFiled: December 22, 2011Date of Patent: August 13, 2013Assignee: Seminis Vegetable Seeds, Inc.Inventor: Joseph Michael Palys
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Publication number: 20130205448Abstract: The present invention relates to methods for expressing proteins of interest, particularly pharmaceutically valuable proteins, transiently in plants. In particular, the invention provides an improved method for introducing Agrobacterium cells into a whole plant or a plant organ. The methods of the invention provides efficient agroinfiltration of many plants singly or simultaneously resulting in a yield of recombinant proteins that is higher than that obtained by other methods. The methods can be readily scaled and automated to meet changing demands of the recombinant protein.Type: ApplicationFiled: July 15, 2011Publication date: August 8, 2013Applicant: PHILIP MORRIS PRODUCTS S.A.Inventors: Andrea Carraro, John Faulkner, Yorick Klipfel, Oleg Mironov, Karen Oishi, Sandrine Roesti
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Publication number: 20130185830Abstract: The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.Type: ApplicationFiled: December 26, 2012Publication date: July 18, 2013Applicant: Monsanto Technology LLCInventor: Monsanto Technology LLC
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Patent number: 8489531Abstract: A method is provided for unsupervised clustering of gene expression data to identify co-regulation patterns. A clustering algorithm randomly divides the data into k different subsets and measures the similarity between pairs of datapoints within the subsets, assigning a score to the pairs based on similarity, with the greatest similarity giving the highest correlation score. A distribution of the scores is plotted for each k. The highest value of k that has a distribution that remains concentrated near the highest correlation score corresponds to the number of co-regulation patterns.Type: GrantFiled: February 2, 2011Date of Patent: July 16, 2013Assignee: Health Discovery CorporationInventors: Asa Ben Hur, Andre Elisseeff, Isabelle Guyon
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Patent number: 8481813Abstract: This invention relates to methods for the transformation of plants from the genus Allium, and transformed plants produced according to the method. Specifically, this invention relates to direct transformation of Allium leaf tissue using Agrobacterium mediated transformation, and plants regenerated from the transformed leaf tissue. In various aspects, the invention relates to a method of obtaining a transformed Allium leaf tissue and methods of obtaining a transformed Allium plant by regenerating the transformed leaf tissue.Type: GrantFiled: October 24, 2008Date of Patent: July 9, 2013Assignee: The New Zealand Institute for Plant and Food Research LimitedInventors: Colin Charles Eady, Fernand Othmar Kenel, Sheree Alma Brinch
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Patent number: 8476491Abstract: The invention relates to a method of utilizing the pts gene and RNA interference of the ads gene to increase patchouli alcohol content in Artemisia annua L. plants. Using transgenic Artemisia annua L. plants, the method of the invention consistently increases the patchouli alcohol content in those plants, thus laying down a solid foundation for large-scale production of patchouli alcohol and other secondary metabolites such as terpenes other than artemisinin.Type: GrantFiled: June 3, 2011Date of Patent: July 2, 2013Assignee: Firmenich Aromatics (China) Co., Ltd.Inventors: Ke Xuan Tang, Yue Yue Wang, Yue Li Tang, Dong-Fang Chen
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Patent number: 8476492Abstract: The invention relates to a method of utilizing the pts gene and antisense ads to increase patchouli alcohol content in Artemisia annua L. plants. Using transgenic Artemisia annua L. plants, the method of the invention consistently increases the patchouli alcohol content in those plants, thus laying down a solid foundation for large-scale production of patchouli alcohol and other secondary metabolites such as terpenes other than artemisinin.Type: GrantFiled: June 3, 2011Date of Patent: July 2, 2013Assignee: Firmenich Aromatics (China) Co., Ltd.Inventors: Ke Xuan Tang, Yue Yue Wang, Yue Li Tang, Dong-Fang Chen
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Publication number: 20130167266Abstract: The present invention provides a heat-resistance plant gene JAZ5a and use thereof. The inventors of the present invention isolated for the first time a heat resistance gene from the plant of Brassica spp., which can greatly improve the heat-resistance ability of the plant, especially in the bolting stage. The present invention further provides a protein encoded by said gene and its preparation method, vectors and host cells containing said gene, and a method for preparing a transgenic plant containing said gene.Type: ApplicationFiled: July 7, 2011Publication date: June 27, 2013Applicant: SHANGHAI INSTITUTES FOR BIOLOGICAL SCIENCES, CHINESE ACADEMY OF SCIENCESInventors: Yu-Ke He, Chuan-Bao Sun
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Publication number: 20130152232Abstract: The present invention provides methods for Agrobacterium-mediated transformation of sugar cane (Saccharum spp.) comprising introducing a nucleotide sequence of interest into a sugar cane callus tissue or cell thereof via Agrobacterium mediated delivery, wherein the sugar cane callus tissue is less than 28 days post-initiation. The invention further provides methods for transforming a sugar cane callus tissue or cell thereof comprising inoculating the sugar cane callus tissue that is less than 28 days post-initiation with an Agrobacterium comprising a nucleotide sequence of interest to produce an Agrobacterium-inoculated sugar cane tissue or cell thereof; and co-cultivating the Agrobacterium and the sugar cane callus tissue to produce a transformed sugar cane callus tissue or cell thereof.Type: ApplicationFiled: June 22, 2011Publication date: June 13, 2013Applicant: Synegenta Participatins AGInventors: Robert Jason Christopher Geijskes, Paulo Cezar De Lucca
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Patent number: 8461419Abstract: Provided are a crop height regulatory gene from Arabidopsis thaliana, expression regulatory sequences thereof and uses thereof. The crop regulatory gene can be used to regulate the plant height, volume, tiller, yield, flower organ size, or seed size of crops.Type: GrantFiled: August 11, 2008Date of Patent: June 11, 2013Assignee: Shanghai Institutes for Biological Sciences, CASInventors: Zuhua He, Yingying Zhang, Qun Li
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Patent number: 8461417Abstract: Reducing /yso-phosphatidylcholine acyltransferase (LPCAT) activity in a plant increases seed oil content and/or fatty acid levels in the plant. Increases in the level of unusual fatty acids 16:3, 18:3 and 20:1 c11 are particularly pronounced.Type: GrantFiled: May 21, 2009Date of Patent: June 11, 2013Assignee: National Research Council of CanadaInventors: Jitao Zou, Qilin Chen, Wenyun Shen, Liping Wang
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Patent number: RE44750Abstract: The present invention relates “disarmed” strain variants of Agrobacterium strain K599 (NCPPB 2659), “disarmed” plasmid variants of the Ri-plasmid pRi2659, and derivatives thereof, and methods employing these strains and plasmids in plant transformation.Type: GrantFiled: September 10, 2012Date of Patent: February 4, 2014Assignee: BASF Plant Science GmbHInventors: Luke Mankin, Dwight-Steven Hill, Paula Olhoft, Effie Toren, Jeffrey A. Brown, Liqun Xing, Huihua Fu, Lesley Ireland, Hongmei Jia, Hee-Sook Song, Allan R. Wenck, Larry Nea