Fluorescence (epo) Patents (Class 850/31)
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Patent number: 10234443Abstract: An apparatus for spectroscopic tissue analysis is disclosed. The apparatus comprises: a light delivery system configured to direct an excitation signal on to a tissue sample; a light collection system configured to collect a backscattered signal comprising diffuse reflectance photons backscattered by the tissue sample; an imaging device; a spectrometer; an optical adaptor configured to direct a first portion of the backscattered signal to the imaging device and a second portion of the backscattered signal to the spectrometer; and an analysis system configured to apply polar decomposition to spectral image data of the tissue captured by the imaging device and the spectrometer and thereby derive polarization metrics for the tissue sample.Type: GrantFiled: March 2, 2018Date of Patent: March 19, 2019Assignee: National University of SingaporeInventor: Zhiwei Huang
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Patent number: 10094766Abstract: According to one aspect, the invention relates to a device (100) for remote polarimetric characterization of a sample (S). It comprises a source (10) for emitting at least one incident light wave at at least one first wavelength (?E); a monomode optical fiber (30) in which the incident light wave is intended to propagate; a polarization state generator (PSG) arranged on the proximal side of the optical fiber; a reflector (40) intended to be arranged on the distal side of the optical fiber; a polarization state analyzer (PSA) arranged on the proximal side of the optical fiber and allowing, for each probe state of the incident wave generated by the polarization state generator, the polarization of the light wave obtained after propagation of the incident wave in the optical fiber (30), reflection from the distal side of the optical fiber and reverse propagation in the optical fiber (30), to be analyzed.Type: GrantFiled: February 18, 2015Date of Patent: October 9, 2018Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE-CNRS, Ecole PolytechniqueInventors: Antonello De Martino, Dominique Pagnoux, Jérémy Vizet, Sandeep Manhas, Jean-Charles Vanel, Stanislas Deby
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Patent number: 8948851Abstract: A tomographic fluorescent imaging device for imaging fluorophores in biological tissues has a scanned laser for scanning the tissue and a camera for receiving light from the biological tissue at an angle to the beam at a second wavelength ten or more nanometers greater in wavelength than the wavelength of the laser. Use of both intrinsic and extrinsic fluorophores is described. Images are obtained at each of several positions of the beam. An image processing system receives the series of images, models a path of the beam through the tissue, and determines depth of fluorophore in tissue from intersections of the modeled path of the beam and the path of the received light. The laser is of 600 nm or longer wavelength, to provide penetration of tissue. The imaging device is used during surgery to visualize lesions of various types to ensure complete removal of malignant tumors.Type: GrantFiled: December 4, 2009Date of Patent: February 3, 2015Assignee: The Trustees of Dartmouth CollegeInventors: Frederic Leblond, David W. Roberts, Brian W. Pogue, Keith D. Paulsen, Alex Hartov, Scott C. Davis, Dax Kepshire
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Patent number: 8528111Abstract: A method for positioning a tip of an atomic force microscope relative to a intracellular target site in a cell is provided. In general terms, the method comprises: a) positioning a fluorescent tip of an atomic force microscope over a cell comprising a fluorescent intracellular target site so that said tip is above target site; b) moving the tip toward said target site while obtaining images of the distal end of said tip and/or the target site using a fluorescence microscope; and c) arresting the movement of the tip when the target site and the distal end of the tip are both in focus in the fluorescence microscope. A microscope system for performing the method is also provided.Type: GrantFiled: July 5, 2011Date of Patent: September 3, 2013Assignee: Agilent Technologies, Inc.Inventors: Nazumi Alice Yamada, Bo U. Curry, Chriatian Rankl
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Patent number: 8429761Abstract: A method of obtaining PINEM images includes providing femtosecond optical pulse, generating electron pulses, and directing the electron pulses towards a sample. The method also includes overlapping the femtosecond optical pulses and the electron pulses spatially and temporally at the sample and transferring energy from the femtosecond optical pulses to the electron pulses. The method further includes detecting electron pulses having an energy greater than a zero loss value, providing imaging in space and time.Type: GrantFiled: September 16, 2010Date of Patent: April 23, 2013Assignee: California Institute of TechnologyInventors: Ahmed H. Zewail, David J. Flannigan, Brett Barwick
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Patent number: 8286260Abstract: A system and method for analyzing and imaging a sample containing molecules of interest combines modified MALDI mass spectrometer and SNOM devices and techniques and includes: (A) an atmospheric pressure or near-atmospheric pressure ionization region; (B) a sample holder for holding the sample; (C) a laser for illuminating said sample; (D) a mass spectrometer having at least one evacuated chamber; (E) an atmospheric pressure interface for connecting said ionization region and said mass spectrometer; (F) a scanning near-field optical microscopy instrument; (G) a recording device for recording topography and mass spectrum measurements made during scanning of the sample with the near-field probe; (H) a plotting device for plotting said topography and mass spectrum measurements as separate x-y mappings; and (I) an imaging device for providing images of the x-y mappings.Type: GrantFiled: May 19, 2010Date of Patent: October 9, 2012Assignee: The George Washington UniversityInventors: Akos Vertes, Mark E. Reeves, Fatah Kashanchi
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Patent number: 7823215Abstract: The present invention relates to near-field scanning optical microscopy (NSOM) and near-field/far-field scanning microscopy methods, systems and devices that permit the imaging of biological samples, including biological samples or structures that are smaller than the wavelength of light. In one embodiment, the present invention permits the production of multi-spectral, polarimetric, near-field microscopy systems that can achieve a spatial resolution of less than 100 nanometers. In another embodiment, the present invention permits the production of a multifunctional, multi-spectral, polarimetric, near-field/far-field microscopy that can achieve enhanced sub-surface and in-depth imaging of biological samples. In still another embodiment, the present invention relates to the use of polar molecules as new optical contrast agents for imaging applications (e.g., cancer detection).Type: GrantFiled: May 12, 2006Date of Patent: October 26, 2010Assignee: The University of AkronInventor: George C. Giakos
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Patent number: 7621964Abstract: An improved near-field scanning optical microscope probe is disclosed. The near-field scanning optical microscope probe includes a probe body and two electrodes extending from the probe body to form a probe tip. In addition, a light-emitting diode is disposed between the two electrodes at the probe tip to act as a light source for the near-field scanning optical microscope probe.Type: GrantFiled: August 31, 2007Date of Patent: November 24, 2009Assignee: The Board of Regents, University of Texas SystemInventors: Kazunori Hoshino, Xiaojing Zhang
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Publication number: 20090119808Abstract: The present invention relates to near-field scanning optical microscopy (NSOM) and near-field/far-field scanning microscopy methods, systems and devices that permit the imaging of biological samples, including biological samples or structures that are smaller than the wavelength of light. In one embodiment, the present invention permits the production of multi-spectral, polarimetric, near-field microscopy systems that can achieve a spatial resolution of less than 100 nanometers. In another embodiment, the present invention permits the production of a multifunctional, multi-spectral, polarimetric, near-field/far-field microscopy that can achieve enhanced sub-surface and in-depth imaging of biological samples. In still another embodiment, the present invention relates to the use of polar molecules as new optical contrast agents for imaging applications (e.g., cancer detection).Type: ApplicationFiled: May 12, 2006Publication date: May 7, 2009Applicant: The University of AkronInventor: George C. Giakos