Abstract: An assembling mechanism of a self-locking linear actuator includes a gearbox, a one-piece rear retainer seat and a casing. The gearbox is composed of two half pieces, which are mated with each other for accommodating and locating a transmission mechanism of the actuator. The rear retainer seat is fixedly held in the gearbox for supporting a bearing of a threaded rod of the transmission mechanism and bearing the stress from the threaded rod. The contact faces of the rear retainer seat and the gearbox are regular polygonal to facilitate adjustment of fixing angle of the rear retainer seat. The casing is composed of two half casings, which are mated with each other to accommodate the transmission mechanism, the gearbox and the rear retainer seat. The mating faces of the half casings are parallel to the axis of the output shaft of a motor to facilitate connection operation of the casing.