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  • Modified Insecticidal Proteins
    Publication number: 20240090511
    Abstract: Provided herein are modified insecticidal proteins comprising an insecticidal protein, wherein the insecticidal protein has been modified to include at least one stink bug gut binding peptide.
    Type: Application
    Filed: November 15, 2021
    Publication date: March 21, 2024
    Applicants: BASF Corporation, University of Florida Research Foundation Incorporated
    Inventors: Razvan Dumitru, Bryony C. Bonning
  • INSECT TOXIN DELIVERY MEDIATED BY A DENSOVIRUS COAT PROTEIN
    Publication number: 20190239513
    Abstract: A coat protein of Junonia coenia densovirus (JcDNV) is used to deliver attached peptide insect toxin across the gut epithelium of a fall armyworm, Spodoptera frugiperda. A fusion protein comprising VP4 attached to an insect toxin via a peptide linker is developed. A composition comprising a JcDNV coat protein attached to an insect toxin via a peptide linker can be used for insect pest control.
    Type: Application
    Filed: September 27, 2017
    Publication date: August 8, 2019
    Inventors: Bryony C. Bonning, Mariah Kemmeree
  • Basement membrane degrading proteases as insect toxins and methods of use for same
    Publication number: 20040123346
    Abstract: Novel insect toxins are disclosed for use in pesticidal compositions and methods. According to the invention basement membrane degrading proteases are identified which are capable of acting as insecticidal agents. Polynucleotides are provided which include expression constructs for the expression of the recombinant insecticidal proteases of the invention as recombinant insect pathogens, as well as transgenic plants with a substantial degree of insect resistance.
    Type: Application
    Filed: October 8, 2003
    Publication date: June 24, 2004
    Inventors: Robert L. Harrison, Bryony C. Bonning
  • INSECT TOXIN DELIVERY MEDIATED BY A DENSOVIRUS COAT PROTEIN
    Publication number: 20210400962
    Abstract: A coat protein of Junonia coenia densovirus (JcDNV) is used to deliver attached peptide insect toxin across the gut epithelium of a fall armyworm, Spodoptera frugiperda. A fusion protein comprising VP4 attached to an insect toxin via a peptide linker is developed. A composition comprising a JcDNV coat protein attached to an insect toxin via a peptide linker can be used for insect pest control.
    Type: Application
    Filed: September 9, 2021
    Publication date: December 30, 2021
    Inventors: Bryony C. Bonning, Mariah Kemmerer
  • Plant virus transmission inhibitor and methods
    Publication number: 20090055975
    Abstract: Provided is a peptide, peptide multimer or fusion protein containing such peptide which binds to the gut of sap-sucking insects, e.g., aphids. When bound, this peptide inhibits the binding of targeted virus to the insect gut. When this peptide or tandem repeat peptide is expressed (or is expressed as part of a fusion protein) in the fluids of transgenic plants, it is taken up by the insect with the sap, binds to gut receptors and thereby inhibits transmission of the virus from plant to plant via the insect vector. Thus, the use of such transgenic plants blocks or reduces the transmission of the targeted virus and others which share the peptide binding site within the insect gut among plants susceptible to the virus and thereby reduces losses due to viral infection.
    Type: Application
    Filed: December 11, 2007
    Publication date: February 26, 2009
    Applicant: Iowa State University Research Foundation, Inc.
    Inventors: Bryony Bonning, W. Allen Miller, Sijun Liu
  • Plant virus transmission inhibitor and methods
    Patent number: 7547677
    Abstract: Provided is a peptide, peptide multimer or fusion protein containing such peptide which binds to the gut of sap-sucking insects, e.g., aphids. When bound, this peptide inhibits the binding of targeted virus to the insect gut. When this peptide or tandem repeat peptide is expressed (or is expressed as part of a fusion protein) in the fluids of transgenic plants, it is taken up by the insect with the sap, binds to gut receptors and thereby inhibits transmission of the virus from plant to plant via the insect vector. Thus, the use of such transgenic plants blocks or reduces the transmission of the targeted virus and others which share the peptide binding site within the insect gut among plants susceptible to the virus and thereby reduces losses due to viral infection.
    Type: Grant
    Filed: December 11, 2007
    Date of Patent: June 16, 2009
    Assignee: Iowa State University Research Foundation, Inc.
    Inventors: Bryony Bonning, W. Allen Miller, Sijun Liu
  • Insect toxin delivery mediated by a densovirus coat protein
    Patent number: 11140902
    Abstract: A coat protein of Junonia coenia densovirus (JcDNV) is used to deliver attached peptide insect toxin across the gut epithelium of a fall armyworm, Spodoptera frugiperda. A fusion protein comprising VP4 attached to an insect toxin via a peptide linker is developed. A composition comprising a JcDNV coat protein attached to an insect toxin via a peptide linker can be used for insect pest control.
    Type: Grant
    Filed: September 27, 2017
    Date of Patent: October 12, 2021
    Assignees: University of Florida Research Foundation, Inc., Iowa State University Research Foundation, Inc.
    Inventors: Bryony C. Bonning, Mariah Kemmerer
  • Basement membrane degrading proteases as insect toxins and methods of use for same
    Patent number: 6673340
    Abstract: Novel insect toxins are disclosed for use in pesticidal compositions and methods. According to the invention basement membrane degrading proteases are identified which are capable of acting as insecticidal agents. Polynucleotides are provided which include expression constructs for the expression of the recombinant insecticidal proteases of the invention as recombinant insect pathogens, as well as transgenic plants with a substantial degree of insect resistance.
    Type: Grant
    Filed: July 12, 2000
    Date of Patent: January 6, 2004
    Assignee: Iowa State University Research Foundation, Inc.
    Inventors: Robert L. Harrison, Bryony C. Bonning
  • Insect diagnostic and control compositions
    Patent number: 5643776
    Abstract: A diagnostic or control composition is useful to characterize or control insects and comprises a nucleotide sequence coding for juvenile hormone esterase (JHE). The coding sequence may be combined with a promoter sequence regulating the transcription thereof in a recombinant expression vector for use in controlling insects having a juvenile hormone esterase dependency. Preferred embodiments of the invention are recombinant baculoviruses in which a mutated JHE coding sequence provides relatively rapid speed of kill in insects.
    Type: Grant
    Filed: August 10, 1992
    Date of Patent: July 1, 1997
    Assignee: The Regents of the University of California
    Inventors: Bruce D. Hammock, Terry N. Hanzlik, Lawrence G. Harshman, Bryony C. Bonning, Vernon K. Ward
  • Viral vector coding for juvenile hormone esterase
    Patent number: 5674747
    Abstract: A diagnostic or control composition is useful to characterize or control insects and comprises a nucleotide sequence coding for juvenile hormone esterase (JHE). The coding sequence may be combined with a promoter sequence regulating the transcription thereof in a recombinant expression vector for use in controlling insects having a juvenile hormone esterase dependency. Preferred embodiments of the invention are recombinant baculoviruses in which a mutated JHE coding sequence provides relatively rapid speed of kill in insects.
    Type: Grant
    Filed: May 12, 1995
    Date of Patent: October 7, 1997
    Assignee: The Regents of the University of California
    Inventors: Bruce D. Hammock, Terry N. Hanzlik, Lawrence G. Harshman, Bryony C. Bonning, Vernon K. Ward
  • Aphicidal Toxins and Methods
    Publication number: 20130097729
    Abstract: Provided are chimeric aphicidal and insecticidal toxin proteins comprising peptide, peptide multimer or fusion protein containing such peptide which binds to the gut of sap-sucking insects, e.g., aphids, thrips, leafhoppers, or other target interest. When bound, this peptide mediates the binding of the chimeric aphicidal or other insecticidal protein to the target insect gut. Also described are coding sequences, vectors, and transgenic plants genetically modified to contain and express such aphicidal or insecticidal proteins. Thus, the use of such transgenic plants reduces economic loss due to feeding by the target insect and also reduces loss due to plant diseases spread by the target insect.
    Type: Application
    Filed: June 8, 2012
    Publication date: April 18, 2013
    Inventors: Bryony Claire Bonning, Sijun Liu, Huarong Li
  • INSECTICIDAL TOXINS FOR PLANT RESISTANCE TO HEMIPTERA
    Publication number: 20180127771
    Abstract: Provided are chimeric Hemiptera-active insecticidal toxin proteins comprising a Bt toxin peptide with activity against Hemiptera, a peptide multimer or fusion protein containing such peptide which binds to the gut of sap-sucking insects, preferably the Asian citrus psyllid, Diaphorina citri. This insect carries a bacterium associated with Huanlongbing disease or citrus greening and damages citrus crops. When bound, this peptide mediates the binding of the chimeric insecticidal protein to the target insect gut. Also described are coding sequences, vectors, and transgenic plants genetically modified to contain and express such insecticidal proteins. Other delivery mechanisms such as use of phloem-inhabiting bacteria, are also contemplated. Thus, the use of these toxins reduces economic loss due to feeding by the target insect and also reduces loss due to plant diseases spread by the target insect.
    Type: Application
    Filed: November 9, 2017
    Publication date: May 10, 2018
    Inventors: BRYONY C. BONNING, NANASAHEB CHOUGULE, MARIA TERESA FERNANDEZ-LUNA, MICHAEL B. BLACKBURN, DAVID G. HALL
  • Plant resistance to insect pests mediated by viral proteins
    Publication number: 20030217385
    Abstract: The invention involves combining a peptide toxin effective against insects, including but not limited to thrips, leaf hoppers, and beetles, with a transport peptide capable of facilitating transfer of the peptide toxin from the gut of an insect to the hemocoel. The combination can be effected by a fusion of genetic material encoding the peptide toxin and the transport peptide, such that expression of the genetic material fusion results in synthesis of a fusion protein combining the functions of both the toxin and the transport protein. Ingestion of the fusion protein by the sucking insect transfers the fusion protein into the insect's gut from which it is transferred into the hemocoel due to the functional activity of the transport peptide where the toxin exerts its toxic effect upon the insect. In a preferred embodiment, the invention is effective in control of such sucking insects as aphids, whiteflies and the like, and other vectors that transmit viruses in a circulative manner.
    Type: Application
    Filed: January 15, 2003
    Publication date: November 20, 2003
    Inventors: W. A. Miller, Bryony C. Bonning
  • Plant resistance to insect pests mediated by viral proteins
    Patent number: 7312080
    Abstract: The invention involves combining a peptide toxin effective against insects, including but not limited to thrips, leaf hoppers, and beetles, with a transport peptide capable of facilitating transfer of the peptide toxin from the gut of an insect to the hemocoel. The combination can be effected by a fusion of genetic material encoding the peptide toxin and the transport peptide, such that expression of the genetic material fusion results in synthesis of a fusion protein combining the functions of both the toxin and the transport protein. Ingestion of the fusion protein by the sucking insect transfers the fusion protein into the insect's gut from which it is transferred into the hemocoel due to the functional activity of the transport peptide where the toxin exerts its toxic effect upon the insect. In a preferred embodiment, the invention is effective in control of such sucking insects as aphids, whiteflies and the like, and other vectors that transmit viruses in a circulative manner.
    Type: Grant
    Filed: January 15, 2003
    Date of Patent: December 25, 2007
    Assignee: Iowa State University Research Foundation, Inc.
    Inventors: W. Allen Miller, Bryony C. Bonning
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