Search Patents
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Patent number: 4977246Abstract: Disclosed is a method of recovery of antihemophilic factor (AHF) from human plasma by precipitation with citric acid, sodium citrate, or potassium citrate.Type: GrantFiled: June 6, 1989Date of Patent: December 11, 1990Assignee: Rorer Pharmaceutical CorporationInventors: Ted C. K. Lee, Michael E. Hrinda
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Patent number: 4894444Abstract: Immunologically intact protein or peptide immunogens are recovered from vaccines consisting of immunogen-aluminum hydroxide (alum) complexes. Recovery consists of dissolution of the complexes with an alkali metal salt of a carboxylic acid at a basic pH, reduction of the pH to physiological levels, removal of excess dissolvent and isolation of the protein or peptide immunogen.Type: GrantFiled: July 16, 1987Date of Patent: January 16, 1990Assignee: Merck & Co., Inc.Inventors: Edgar M. Scattergood, Roy W. Grabner
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Patent number: 5179196Abstract: The present invention provides a process for the recovery of heterologous proteins from CTAP-III fusion proteins comprising expressing a fusion protein having a first amino acid sequence, a second amino acid sequence, and a selectable site which may be cleaved to provide first and second polypeptide fragments, respectively, wherein the first amino acid fragment is homologous to CTAP-III, and the first and second fragments have different pI values; cleaving the fusion protein to provide the first and second fragments; and separating the first and second fragments by ion exchange chromatography.Type: GrantFiled: May 4, 1989Date of Patent: January 12, 1993Assignee: SRI InternationalInventors: Paul H. Johnson, Ping Sze, Richard C. Winant, Jerome B. Lazar
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Patent number: 5064943Abstract: A method for solubilization and naturation of somatotropin using an aqueous alkaline solution results in lower dimer formation and eliminates denaturants and separate renaturation steps and agents.Type: GrantFiled: December 16, 1988Date of Patent: November 12, 1991Assignee: American Cyanamid CompanyInventors: Kevin M. McCoy, Robert A. Frost
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Patent number: 5003047Abstract: A method for purifying a biologically active ligate. In this method, a ligand bonded to a first phase and having a specific affinity for the ligate is provided. The ligate is provided with a second phase. The first and second phases are then contacted together under conditions in which the ligand and ligate form a complex bonded to the first phase, with the ligand and ligate held together only by one or more non-covalent pressure sensitive bonds. At least a part of the second phase is then separated from the first phase to provide a purified first phase, and the purified first phase subjected to a pressure of at least 300 atmospheres under conditions sufficient to cause release of the ligate from the complex, but not sufficient to cause significant release of the ligand from the first phase. These conditions do not irreversibly cause biological activity of the ligate to be significantly reduced.Type: GrantFiled: January 10, 1989Date of Patent: March 26, 1991Assignee: Massachusetts Institute of TechnologyInventors: Martin L. Yarmush, William C. Olson
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Patent number: 4985544Abstract: Reactivation of cysteine-containing protein in a process, in which a reduced and denatured cysteine-containing protein such as salmon growth hormone I or eel growth hormone I can be efficiently reactivated.Type: GrantFiled: August 2, 1988Date of Patent: January 15, 1991Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Yoshiharu Yokoo, Seiji Sugimoto
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Patent number: 4975529Abstract: A method of recovering biologically active somatotropins which are produced as insoluble refractile bodies in transformed microorganisms which comprises dissolving refractile bodies in a denaturant and 2-amino-2-methyl-1-propanol, homogenizing, and diluting to fold.Type: GrantFiled: August 18, 1989Date of Patent: December 4, 1990Assignee: Monsanto CompanyInventors: Ronald B. Frazier, Yasuo Konishi
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Patent number: 4897465Abstract: Disclosed are ultrafiltration processes for the enrichment and concentration of selected proteins from protein conaining fluids. Illustratively, IgG is concentrated from whey by means of multiple ultrafiltration "passes", using a metallic oxide ultrafiltration membrane, which are characterized by differing pH's.Type: GrantFiled: October 12, 1988Date of Patent: January 30, 1990Assignee: Abbott LaboratoriesInventors: Christopher T. Cordle, Larry G. Criswell, Ronald L. Thomas
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Patent number: 5116952Abstract: Substantially pure proteins active in humoral hypercalcemia of malignancy (PTHrP) and sub-units and fragments thereof. Antibody reagents capable of binding to epitopes of PTHrP. Methods and kits for the detection of PTHrP.Type: GrantFiled: May 9, 1988Date of Patent: May 26, 1992Assignee: The University of MelbourneInventors: Thomas J. Martin, Jane M. Moseley, Bruce E. Kemp, Richard E. H. Wettenhall
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Patent number: 5122597Abstract: The present invention relates to the application, as the stationary phase in affinity chromatography for the purification of the FGF type growth factors, of the polymers or copolymers onto which --SO.sub.3 H and --SO.sub.3 M groups are randomly bound, groups in which M denotes a physiologically acceptable metal, as well as, preferably, --SO.sub.2 R groups, in which R denotes a radical obtained by removal of a hydrogen atom from the amino group of an amino acid or an amino acid derivative.The invention relates also to the obtaining of a purified bFGF or aFGF growth factor, or a mixture of these growth factors, consisting in carrying out an affinity chromatography on a resin such as defined hereinabove, by carrying out an elution with a neutral pH buffer having an ionic strength equivalent to that of a 0.15 M to 2.5 M NaCl solution.Type: GrantFiled: April 15, 1988Date of Patent: June 16, 1992Assignee: Centre National de la Recherche Scientifique (CNBS)Inventors: Denis S. C. Barritault, Josette Badet nee Genissel, Jose P. Courty, Marie-Anne Dourges nee Jacquot, Danielle Gulino nee Debrac, Jacqueline Jozefonvicz nee Dorgebray
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Patent number: 5245012Abstract: A recombinant spider silk protein can be obtained in a commercially useful form by cloning and the expression in a host cell of a polynucleotide encoding an endogenous spider silk protein or variant thereof. The sequencing of a spider silk protein is made possible by a method for solubilizing a spider silk protein.Type: GrantFiled: September 29, 1992Date of Patent: September 14, 1993Assignee: The United States of America as represented by the Secretary of the ArmyInventors: Stephen J. Lombari, David L. Kaplan
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Patent number: 5268363Abstract: A purified protein, factor J, which has inhibitory properties which prevent the formation or the dissociation of Cl complex and a method of purification for said protein. The method including the following sequential chromatography steps: anion exchange QAE-"SEPHADEX", heparin-"SEPHAROSE" affinity, "MONO Q" and hydroxylapatite.Type: GrantFiled: April 27, 1992Date of Patent: December 7, 1993Assignee: The Beth Israel Hospital AssociationInventor: Anne Nicholson-Weller
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Patent number: 5008377Abstract: A method for the preparation of a protein in a physiologically active or native form, which method includesproviding a source of protein in a solubilized form,and a cationic exchange medium;contacting the source of protein and cationic exchange medium; andrecovering the protein in a physiologically active form.Type: GrantFiled: April 21, 1988Date of Patent: April 16, 1991Assignee: Bunge (Australia) Pty. Ltd.Inventors: Joseph J. Patroni, Malcolm R. Brandon
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Patent number: 5128453Abstract: Purification of human FSH from post-menopausal urine gonadotropin using immunoaffinity chromatography with elution at high pH and reverse-phase HPLC steps yields a biologically active hormone which is free from detectable traces of luteinizing hormone and other urinary proteins.Type: GrantFiled: February 7, 1989Date of Patent: July 7, 1992Assignee: Istituto DiRicerca Cesare Serono SpAInventors: Guiseppe Arpaia, Serenella Serani, Antonino Sirna, Stefano Villa
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Patent number: 5077390Abstract: A substantially pure, hemagglutinin-free composition of trichosanthin, and a method of producing the composition is disclosed. A plant extract from Trichosanthes kirilowii is contacted with an anionic exchange resin to remove contaminating hemagglutinins, and trichosanthin is further purified from the extract by cation exchange chromatography to a purity of greater than 95%.Type: GrantFiled: September 7, 1989Date of Patent: December 31, 1991Assignee: Genelabs, IncorporatedInventors: Paul S. Wu, Susan B. Wade, Raul R. Soikes
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Patent number: 4992531Abstract: A method for the recovery of proteins in a solubilized form from host cells including providing a source of host cells incorporating a synthesized or expressed protein; providing a source of at least one cationic surfactant; and treating the host cells with at least one cationic surfactant, in an amount sufficient to effect solubilization of the proteins.Type: GrantFiled: June 13, 1988Date of Patent: February 12, 1991Assignee: Bunge (Australia) Pty. Ltd.Inventors: Joseph J. Patroni, Malcolm R. Brandon
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Patent number: 4981952Abstract: The recovery of vitamin K-dependent proteins produced by transformed microorganisms can be effected from the cell culture medium utilizing the changes in the protein which occur in the presence of divalent cations. The present process uses divalent cations to alter the binding affinity of the proteins and thereby selectively elute the proteins away from contaminants in the culture medium using standard chromatography.Type: GrantFiled: August 16, 1989Date of Patent: January 1, 1991Assignee: Eli Lilly and CompanyInventor: S. Betty Yan
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Patent number: 4966963Abstract: A method for the preparation of proteins in biologically active form including providing a source of protein solubilized from inclusion bodies with a cationic surfactant; providing a weak denaturing agent; and contacting the solubilized protein with the weak denaturant in water in an amount sufficient to allow the protein to remain in a biologically active form.Type: GrantFiled: February 11, 1988Date of Patent: October 30, 1990Assignee: Bunge (Australia) Pty. Ltd.Inventor: Joseph J. Patroni
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Patent number: 4983722Abstract: Protein A is selectively isolated from an antibody--Protein A mixture by exposing the mixture to an anion exchange material under conditions sufficient to adsorb both components and then sequentially eluting the antibodies and protein A under conditions of increasing ionic strength. Resulting antibody preparations have less than about 15 ng of Protein A per mg of antibody.Type: GrantFiled: June 8, 1988Date of Patent: January 8, 1991Assignee: Miles Inc.Inventors: James W. Bloom, Melvin F. Wong, Gautam Mitra
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Patent number: 5415859Abstract: The protein doublet H110D, the individual components thereof, and the production and use thereof in a vaccine against a nematode infection. This protein doublet is a plasma membrane-associated protein material of the intestinal microvilli of Haemonchus contortus. H110D has a molecular weight of about 110 kd and reacts with antibodies raised in animals injected with a contortin-enriched fraction. Injection of preparations of the protein doublet H110D or its components induces the production of specific protective antibodies.Type: GrantFiled: August 14, 1992Date of Patent: May 16, 1995Inventor: Edward A. Munn