Abstract: A process for forming an integrated multiplex electrospray includes forming multiple holes in a ring extractor substrate to create a ring extractor. A nozzle array having multiple nozzles each nozzle defining a central axis is provided. A spacer layer is bonded to either the ring extractor or the nozzle array to form a bonded stack. The bonded stack is then aligned with remaining layer to align each of the multiple nozzles with one of the plurality of holes to less than 10 microns from concentric and the spacer layer then bonded intermediate between the ring extractor and the nozzle array layer. The spacer layer is then etched to provide fluid communication between multiple nozzles and the multiple holes of the ring extractor and form the spacer.

Abstract: Novel immunoregulatory utilities of Escherichi coli heat-labile enterotoxin (LT) are disclosed. This enterotoxin can be used in combination with an unrelated antigen to achieve a higher immune response to said antigen when administered as part of an oral vaccine preparation. By way of example, the efficacy of oral adjuvant therapy of LT in the development of immunological protection against herpes simplex virus was examined. In addition, the ability of LT to influence the induction and maintenance of tolerance in animals primed orally with two unrelated protein antigens administered simultaneously, OVA and BSA was examined. Simultaneous administration of LT with OVA was shown to prevent the induction of tolerance to OVA and to increase the serum anti-OVA IgG response to 30-90 fold over PBS primed and OVA primed animals, respectively.

Abstract: A first embodiment is a specific plasmid vector, pDIP/PyCSP.1, into which nucleotides encoding the targets of specific immune responses are inserted. These targets include, but are not limited to proteins and peptides. These plasmid constructs are incorporated in a composition comprising a suitable and acceptable art recognized pharmaceutical reagent that is benign (non-reactive with) to the plasmid construct. The plasmid construct provides protective immune responses to the disease associated with the selected targets. A second embodiment is a construct having, at a minimum, the nucleotide sequences encoding one or more Plasmodium species proteins in a pharmaceutically acceptable vector. a third embodiment is a method of controlling malaria in mammals comprising injecting a polynucleotide delivery vector into a mammal.

Abstract: An assay method and kit for detecting specific oral cariogenic bacteria, ., mutans streptococci, Lactobacillus sp. and Actinomyces sp., separately or in combination, comprising gathering a sample suspected of containing cariogenic bacteria; treating the sample with a stripping buffer to remove host antibodies from bacteria present in the sample; retaining the treated bacteria on a blocked solid phase substrate; reacting the retained bacteria with a primary antibody specific for the desired cariogenic bacteria; reacting the primary antibody with a conjugated label producing a detectable signal; and detecting the signal whereby the presence of the desired cariogenic bacteria is determined in the sample. The device for conducting these assays is a frame or support which holds a solid substrate capable of retaining the bacteria of interest while permitting drainage of other materials or fluids away from the retained bacteria.

Abstract: Decompression from dives using nitrogen or hydrogen as a dilutent gas are celerated by introducing into the large intestine an enzyme or, preferably non-toxic bacteria from the group that metabolizes hydrogen or from the group that metabolizes nitrogen. The bacteria are encouraged to multiply and feed on the hydrogen or nitrogen (dependent on the gas mixture used in the dive) by metabolizing the diluent gas released into the large intestine and the new product is vented from the large intestine. The metabolism of the hydrogen or nitrogen causes a reduction of the partial pressure of the metabolized gas in the large intestine thereby increasing the diffusion of the metabolized gas from the blood and surrounding tissues into the intestine. The delivery of the bacteria is accomplished by any one of several means with packaging of the enzyme or bacteria in enteric coatings for oral ingestion as a prefered means.

Abstract: A method for ameliorating the effects of hemorrhagic shock in a subject consists of locally administering to the gut of the subject, at a selected time during the period of from about 5 hours before blood loss to about 5 hours after blood loss, a therapeutic amount of a cytokine.

Abstract: A recombinant fusion protein (DEN-2 MBP) containing the B domain of the due (DEN 2 envelope protein is disclosed as a candidate subunit immunogen for vaccination against dengue virus infection. A gene fragment encoding amino acid 298 to amino acid 400 of the DEN-2 virus envelope was expressed as a fusion protein with the maltose binding protein (MBP) of Escherichia coli (E. coli). The recombinant fusion protein was purified and analyzed for its antigenicity imunogenicity and ability to protect mice against lethal challenge. This antigen is detected by monoclonal antibody (3H5) which is specific for a neutralizing epitope on the DEN-2 envelope and reacted with homologous polyclonal mouse immune ascitic fluid and DEN-2 immune human sera. A recombinant fusion plasmid bearing the DEN-2 MBP DNA sequence, expressing the fusion product in E. coli is disclosed.

Abstract: A dental waste collection method and system in which a slurry of water and olid waste particles, including amalgam containing mercury and soluble and suspended mercury and other heavy metals is fed into a settling tank in which particles of insoluble solid waste material settle by gravity for later extraction, disposal, or reclamation. The liquid wastes and suspended metal particulates also can be co-precipitated to remove small size insoluble particles entrained in the slurry and soluble mercury and other metals. The supernatant remaining in the tank is removed and passed through a treatment stage including at least one filter and, if needed ion exchange media, and is in a form clarified of the mercury such as to be able to be conveyed into a public sewer system.

Abstract: Fluorescent-labeled substrates are provided for fluorescence polarization says of enzymes. These substrates are proteins labeled with derivatives of BODIPY.RTM., 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene. The BODIPY.RTM. fluorescent tag of the present invention is pH independent, and can be used over a pH range of from about 2 to about 11. Thus one can assay, in real time, enzymes with pH maxima at pH below 7 using fluorescence polarization methodology, which could not be done with fluorescein derivatives. Different enzymes can be compared using the same BODIPY.RTM. conjugate by merely changing the buffer system which changes the pH conditions.Fluorescence polarization assays of enzyme activity can be performed in the presence of whole bacteria and other finely suspended particles, such as might be present in tissue homogenates or cellular material. This is particularly useful for chairside assays on dental plaque or clinical assays on bacteria or tissue or exudates.

Abstract: A method and apparatus to treat dental waste water has been developed in ch a slurry of water and solid waste particles, including mercury-containing amalgam and soluble and suspended mercury and other heavy metals, is collected under vacuum into an main chamber. The particulate-containing liquid slurry settles into a main chamber and into the central collection well of a removable filter while the vacuumed air exits the main chamber and continues toward a vacuum source. The liquid slurry is drawn by vacuum through a removable filter making the effluent substantially free of mercury-containing particulate material. The resulting filtered effluent is ready for further treatment of soluble and suspended mercury, if necessary, before being conveyed into a public sewer system. When a certain level of particulate material has been collected, the removable filter can be sealed and the particulate contents disposed or reclaimed.

Abstract: An assay for detecting microbial protease activity in clinical and laboray samples is described which comprises gathering a sample suspected of containing certain microorganisms having the desired protease activity; immobilizing the microorganisms in the sample on a solid phase substrate; contacting the immobilized microorganisms with an enzymatic substrate producing an enzymatic substrate end-product; contacting the enzymatic substrate end-product with a chemical enhancing reagent producing a detectable chromogenic reaction which varies in intensity with the level of protease activity in the sample; and detecting the chromogenic reaction whereby the semi-quantitative presence of the protease activity in the sample is determined.

Abstract: A monoclonal antibody is disclosed which is reactive to Bacteroides gingivalis and produced by the hybridoma deposited under ATCC HB 9968. The invention also discloses diagnostic reagents and methods for detecting Bacteroides gingivalis utilizing the hybridoma deposited under ATCC HB 9968.

Abstract: Decompression from dives using nitrogen or hydrogen as a dilutent gas are accelerated by introducing into the large intestine an enzyme or, preferably non-toxic bacteria from the group that metabolizes hydrogen or from the group that metabolizes nitrogen. The bacteria are encouraged to multiply and feed on the hydrogen or nitrogen (dependent on the gas mixture used in the dive) by metabolizing the diluent gas released into the large intestine and the new product is vented from the large intestine. The metabolism of the hydrogen or nitrogen causes a reduction of the partial pressure of the metabolized gas in the large intestine thereby increasing the diffusion of the metabolized gas from the blood and surrounding tissues into the intestine. The delivery of the bacteria is accomplished by any one of several means with packaging of the enzyme or bacteria in enteric coatings for oral ingestion as a prefered means.

Abstract: A self-contained personal microwave and RF detector, which includes, inter alia, the housing and associated electronics of a standard hearing aid, is configured to produce an audible indication to a wearer thereof in response to electromagnetic fields of dangerously high levels within predetermined frequency ranges.

Abstract: Antisense oligonucleotides complementary to human mRNAs or pre-mRNAs coding or VCAM-I are used in a therapeutic treatment of sepsis (sepsis, the sepsis syndrome, septic shock and all other manifestations of the sepsis disease, including but not inclusive of, adult respiratory distress syndrome, multi-organ failure, or cardiovascular dysfunction).

Abstract: The present invention provides a method for the treatment of septic shock d inflammatory complications of shock. A process for selectively inhibiting the expression of the human ELAM-I mRNA transcript using at least one oligonucleotide which is substantially complementary to at least a portion of the ELAM-I gene is disclosed, as are composition comprising the oligonucleotide.

Abstract: The present invention provides a method for the treatment of septic shock and inflammatory complications of shock. A process for selectively inhibiting the expression of the human ICAM-I mRNA transcript using at least one oligonucleotide which is complementary to at least a portion of the human ICAM-I mRNA is disclosed, as are oligonucleotides which are complementary to portions of the ICAM-I mRNA and compositions comprising the oligonucleotides.