Abstract: Glycerides containing methylene-interrupted polyunsaturated fatty acid moieties are prepared by reacting a lower alkyl ester of the corresponding polyunsaturated fatty acid or desired mixture of fatty acids with glycerol in the presence of alkoxide ion as the catalyst and removing the lower alcohol as it is formed, preferably at a pressure below atmospheric pressure. Triglycerides of omega-3 fatty acids, such as EPA and DHA, were prepared and triglycerides of mixed polyunsaturated and monounsaturated or short or medium chain fatty acids can also be prepared using that approach.