Abstract: Methods are provided for the establishment and maintenance in long term culture of hormone secreting cells. Cells are derived from tumorous or non-tumorous animal or human tissues, including ovary, endometrium, trophoblast, pituitary, thyroid, and pancreas. The cells secrete into the culture medium hormones such as estrogens, progestins, follicle-stimulating hormone, luteinizing hormone, human chorionic gonadotrophin, thyroxin, glucagon, and insulin, depending on the tissue of origin of individual cell cultures. Contact with an appropriate secretogogue causes the cells to respond with increased hormone secretion. For instance, ovarian follicular cells respond to follicle-stimulating hormone with increased estrogen and progesterone secretion. Pancreatic cells respond to elevated glucose with increased insulin secretion. The cells proliferate in in vitro for up to one year or longer, during which time they retain their hormone-secretion profile.

Abstract: A stowable cargo restraining enclosure assembly (10, 110) for restraining cargo (20) in a cargo space (30) of a transport vehicle (40). The enclosure assembly preferably includes a bed liner structure (70, 170) that may be positioned on the bed (50, 150) of the cargo space and at least one enclosure assembly that includes a plurality of sidewalls (80A, 80B, 90A, 90B, 180A, 180B, 190A, 190B) which are pivoted and/or folded into a collapsed or stowed condition in the bed liner (50, 150). During use, the enclosure assembly (10, 110) is pivoted up into the cargo space (30) and the sidewalls deployed to cooperatively form a walled enclosure for cargo. A transport vehicle (40) having the cargo restraining enclosure assembly (10, 110) and a method of restraining cargo in a transport vehicle also are disclosed.