Abstract: Methods and compositions are provided for recombinant DNA production of Factor VIIIC and truncated derivatives thereof. Based on amino acid sequences, probes are developed for isolating messenger RNA and/or chromosomal DNA encoding for Factor VIIIC. The Factor VIIIC gene in its entirety or encoding for a fragment thereof is then used for expression of Factor VIIIC in a host. The bacteriophage .lambda.FVIII23D containing the 14.43 kb EcoRI fragment was deposited at the A.T.C.C. on Jan. 4, 1984 and given Accession No. 40094.
Type:
Grant
Filed:
October 26, 1993
Date of Patent:
July 21, 1998
Assignees:
Chiron Corporation, Novo Nordisk A/S
Inventors:
George Kuo, Frank Masiarz, Martha Truett, Pablo Valenzuela, Mirella Ezban Rasmussen, Jennifer M. Favaloro
Abstract: A composition for the treatment of osteoporosis and the method of treatment of osteoporosis therewith in mammals is disclosed. The method comprises administering Insulin-Like Growth Factor I (IGF-I) to a mammal in need thereof. Need is determined by bone mineral density levels, whether by single measurement (as compared to the population generally) or by a series of measurements evidencing a loss of bone mineral density, or due to a genetic or familial or other predisposition to bone mineral density loss.
Abstract: The isolation, identification and production by recombinant methods of bone calcification factor, a 22 KD polypeptide, are disclosed. The peptide has calcification-inducing activity when implanted with matrix Gla protein into mammals.
Type:
Grant
Filed:
May 4, 1994
Date of Patent:
June 3, 1997
Assignee:
Chiron Corporation
Inventors:
Michael C. Kiefer, Frank R. Masiarz, Philip J. Barr
Abstract: DNA encoding two forms of PDGF A-chain polypeptide, the construction of expression vectors for expressing such DNA in yeast and mammalian cells, and the expression of such DNA in yeast and mammalian cells to produce active PDGF A-chain homodimer and active PDGF A-chain/B-chain heterodimer are disclosed.
Type:
Grant
Filed:
May 30, 1995
Date of Patent:
February 25, 1997
Assignee:
Chiron Corporation
Inventors:
Carl-Henrik Heldin, Christer Betsholtz, Bengt Westermark, Timothy J. Knott, James Scott, Graeme I. Bell
Abstract: A yeast .alpha.-factor expression system is provided comprised of a truncated leader sequence, containing the .alpha.-factor signal peptide and one glycosylation site, linked by a processing site to a non-yeast protein-encoding sequence.
Abstract: EGF muteins in which the histidine at position 16 is replaced with a neutral or acidic amino acid exhibit activity at pHs lower than obtainable with wild type EGF.
Type:
Grant
Filed:
August 2, 1994
Date of Patent:
August 20, 1996
Assignee:
Chiron Corporation
Inventors:
Guy T. Mullenbach, Jeffrey M. Blaney, Steven Rosenberg
Abstract: Methods and compositions are provided for the production of human superoxide dismutase and a novel protocol for enhancing efficiency of expression. The gene encoding for human superoxide dismutase is isolated and inserted into a vector in conjunction with a synthetic linker which provides for enhanced efficiency in translation.E. coli strain D1210 (pSOD.times.8) was deposited at the A.T.C.C. on Sep. 27, 1983 and given Accession No. 39453. Yeast strain 2150-2-3 (pC1/1GAPSOD) and E. coli strains D1210 (pSOD11) and D1210 (pS2OR) were deposited at the A.T.C.C. on May 9, 1984, and given Accession Nos. 20708, 39679 and 39,680, respectively.
Abstract: DNA encoding two forms of PDGF A-chain polypeptide, the construction of expression vectors for expressing such DNA in yeast and mammalian cells, and the expression of such DNA in yeast and mammalian cells to produce active PDGF A-chain homodimer and active PDGF A-chain/B-chain heterodimer are disclosed.
Type:
Grant
Filed:
August 27, 1990
Date of Patent:
June 15, 1993
Assignee:
Chiron Corporation
Inventors:
Carl-Henrik Heldin, Christer Betsholtz, Bengt Westermark, Timothy J. Knott, James Scott, Graeme I. Bell