Patents Represented by Attorney, Agent or Law Firm Bertram I. Rowland
  • Carboxyl-terminal protein sequencing method and kit
    Patent number: 5064767
    Abstract: Polypeptides are sequenced from the carboxyl terminus by treating the peptide with an iodoxybenzene, followed by removal of the excess reagent and reaction with 2-aminothiophenol at an elevated temperature. The resulting benzthiazolidine is then analyzed to determine the C-terminal amino acid which has been cleaved. A kit which contains the iodoxybenzene and 2-aminothiophenol reagents is separate containers is also disclosed.
    Type: Grant
    Filed: June 21, 1990
    Date of Patent: November 12, 1991
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Dean A. Le, Kazuhiko Tatemoto
  • Retroviral polypeptides associated with human cellular transformation
    Patent number: 5063150
    Abstract: Polypeptides, fragments thereof, antisera and monoclonal antibodies thereto are provided for the detection of retroviruses in the HTLV family capable of inducing transformation in human lymphocytes and other immune cells, regulation of viral replication and transformation or infection of human cells.
    Type: Grant
    Filed: November 13, 1987
    Date of Patent: November 5, 1991
    Assignee: The Regents of The University of California
    Inventors: Dennis J. Slamon, Irvin S. Y. Chen
  • Human hematopoietic stem cell
    Patent number: 5061620
    Abstract: Human hematopoietic stem cells are provided by separation of the stem cells from dedicated cells. The stem cells may than be maintained by regeneration in an appropriate growth medium. Means are provided for assaying for the stem cells as to their capability for producing members of each of the hematopoietic lineages.
    Type: Grant
    Filed: March 30, 1990
    Date of Patent: October 29, 1991
    Assignee: SyStemix, Inc.
    Inventors: Ann Tsukamoto, Charles M. Baum, Yukoh Aihara, Irving Weissman
  • Fluorescent conjugates for analysis of molecules and cells
    Patent number: 5055556
    Abstract: Sensitive detection techniques and compositions for such techniques are provided by employing fluorescent proteins having bilin prosthetic groups as labels. The bilin containing proteins can be conjugated to ligands or receptors for use in systems involving ligand-receptor binding for the analysis, detection or separation of ligands and receptors. Particularly, one or more of the bilin containing proteins may be used as labels in conjunction with each other or other fluorescers for defining subsets of naturally occurring aggregations e.g. cells.
    Type: Grant
    Filed: October 25, 1985
    Date of Patent: October 8, 1991
    Assignee: The Board of Trustees of the Leland Stanford Jr. Univ.
    Inventors: Lubert Stryer, Alexander N. Glazer, Vernon T. Oi
  • Intimate mixture of calcium and phosphate sources as precursor to hydroxyapatite
    Patent number: 5053212
    Abstract: Novel compositions are provided for the production of hydroxyapatite, where a dry mixture is provided which combines a calcium source and an acidic phosphate source, particularly monocalcium phosphate monohydrate or orthophosphoric acid crystals. These two are combined, optionally with other ingredients, with mechanical mixing, resulting in partial reaction or intimate combination to produce a product which requires less water for formation of a desired viscosity, has enhanced mechanical properties and is physilogically acceptable.
    Type: Grant
    Filed: July 27, 1990
    Date of Patent: October 1, 1991
    Assignee: Norian Corporation
    Inventors: Brent R. Constantz, Bryan Barr, Kevin McVicker
  • In situ calcium phosphate minerals method
    Patent number: 5047031
    Abstract: Calcium phosphate minerals are formed by using phosphoric acid substantially free of uncombined water in conjunction with a calcium source, normally as any combination of carbonate, phosphate and hydroxide, and, as required, any additional base to neutralize the phosphoric acid. Protein may be optionally added. The resulting product is readily formed and then sets to a hard, stable, workable shaped object.
    Type: Grant
    Filed: May 30, 1989
    Date of Patent: September 10, 1991
    Assignee: Norian Corporation
    Inventor: Brent R. Constantz
  • Intronic overexpression vectors
    Patent number: 5043270
    Abstract: DNA constructs are provided employing intronically positioned expression, systems, where one of the genes is a dominant gene, usually amplifiable, and the other gene encodes a sequence of interest. Higher levels of co-expression are achieved than when the genese are ligated in tandem. Specifically, the gene of interest is inserted into the intron of a DHFR minigene, the construct transformed into a mammalian cell and the resulting transformants stressed with progressively increasing levels of methotrexate. Substantially increasing levels of co-expression are achieved with increasing levels of methotrexate.
    Type: Grant
    Filed: March 31, 1989
    Date of Patent: August 27, 1991
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: John M. Abrams, Robert T. Schimke, Susan M. Thorpe
  • Secretory signal selection vectors for extracellular protein synthesis bacilli
    Patent number: 5037760
    Abstract: Novel vectors are provided for identifying secretory signal sequences from DNA fragments of unicellular microorganisms. The plasmids comprise a multiple cloning site with restriction sites in reading frame with a structural gene which permits rapid screening of the screted expression product. Optionally, the vectors may include a promoter region upstream from the multiple cloning site. The invention is exemplified with Bacillus. Specific secretory signal sequence have been isolated with those vectors, allowing for efficient secretion into the supernatant, and not just to the periplasmic space to provide proteins in economically high yields. Secretory sequences are provided superior to other previously known sequences.
    Type: Grant
    Filed: May 1, 1987
    Date of Patent: August 6, 1991
    Assignee: Gist-Brocades NV
    Inventors: Hilde E. Smith, Jan H. Van Ee, Ben P. H. Peeters, Sierd Bron, Gerard Venema
  • Composition comprising octacalcium phosphate crystals and polypeptide
    Patent number: 5034059
    Abstract: Octacalcium phosphate and hydroxyapatite are provided, where octacalcium phosphate is produced as novel single crystals and organized polycrystalline structures that are non-dense. The crystals serve as fibers, which find application in a wide variety of applications as well as coatings of prosthetic devices or other materials which may have physiological application.
    Type: Grant
    Filed: February 17, 1989
    Date of Patent: July 23, 1991
    Assignee: Norian Corporation
    Inventor: Brent R. Constantz
  • Area-modulated luminescence (AML)
    Patent number: 5026159
    Abstract: A luminescence measuring system is provided for detecting luminescence at extremely low concentrations of luminescing moieties. The method employs alternating radiation at a plurality of loci of an inhomogenous solution, where the radiant power is maintained constant, and the irradiated volumes of pairs of loci are systematically varied. With the probability being very low that the same luminescence signal will be obtained in the two or more measurements, by comparing the measurements, one can detect a low luminescence signal in the presence of relatively high noise levels. Various techniques are described for modulating the irradiance and detecting changes in signal.
    Type: Grant
    Filed: May 19, 1989
    Date of Patent: June 25, 1991
    Assignee: Acrogen, Inc.
    Inventors: Fritz S. Allen, Carlos Bustamante, Thomas M. Niemczyk, Burton P. Dorman
  • Conjugates of a vinca derivative carrying a detergent chain in the C-3 position
    Patent number: 5024835
    Abstract: The subject of the present invention is conjugates of vinca (indole-dihydroindole) derivatives carrying a detergent chain of at least 7 aliphatic carbon atoms in the C-3 position bonded via a covalent bond to a macromolecular carrier of polypeptide character, and also pharmaceutical compositions containing these conjugates and a process of preparation.
    Type: Grant
    Filed: February 3, 1989
    Date of Patent: June 18, 1991
    Assignee: Ire-Celltarg S.A.
    Inventors: KSP Siva Bhushana Rao, Jean-Paul Dejonghe, Marie-Paule Collard, Andre Trouet
  • Regulatory region cloning and analysis plasmid for bacillus
    Patent number: 5024943
    Abstract: Novel constructs in plasmids are provided for evaluating the efficiency of expression and secretion of structural genes. The constructs provide for transcriptional and translational regulatory regions, a signal sequence and structural gene, which also may be readily excised and substituted, so as to allow for mixing and matching of regulatory regions, signal sequences and genes to evaluate regions for use in the expression of a desired peptide. Particularly, synthetic regions are provided which may be used with other synthetic regions or wild-type regions.Plasmids are provided for screening Bacillus genomic sequences for regulatory regions, particularly promoters employing a structural gene secreting an enzyme which can produce a product which allows for visual detection.
    Type: Grant
    Filed: November 4, 1986
    Date of Patent: June 18, 1991
    Assignee: Gist-brocades
    Inventor: Jan H. Van Ee
  • In situ transcription in cells and tissues
    Patent number: 5021335
    Abstract: In situ transcription is provided by hybridizing cells or tissue with a primer, and extending the primer bound to any template mRNA or DNA with reverse transcriptase or DNA polymerase in the presence of labeled nucleotides, which allows for detection of cells containing the template. The resulting cDNA may be eluted and used in a polymerase chain reaction for isolation of the DNA, and/or the cDNA electrophoresed, which may provide for information concerning the sequence, or the like.
    Type: Grant
    Filed: June 17, 1988
    Date of Patent: June 4, 1991
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Laurence Tecott, Jack D. Barchas, James Eberwine
  • Immunonodulating compositions and their use
    Patent number: 5019384
    Abstract: Novel methods or compositions are provided for modulating the immune system, so as to be able to selectively stimulate or inactivate lymphocytes in relation to a particular transplantation antigen content. Particularly, mixtures may be employed associated with the more common transplanatation antigens of a host population. In this manner, a large number of people can be treated, for example, by immunization, stimulation of particular T-cells or B-cells in relation to a pathogenic invasion of other aberrant state, e.g. neoplasia, treatment of autoimmune diseases, and the like. Particularly, the compositions may involve an oligopeptide involving as a first region a consensus sequence and an epitope or the first region may be joined to a second region comprising an antibody target sequence which is capable of competing with an epitopic site of an antigen of interest.
    Type: Grant
    Filed: November 13, 1989
    Date of Patent: May 28, 1991
    Assignee: Massachusetts Institute of Technology
    Inventors: Malcolm L. Gefter, Jean G. Guillet
  • Human squamous lung carcinoma cell specific antigens and antibodies
    Patent number: 5019497
    Abstract: Methods and compositions are provided for detecting antigens having a specific epitope associated with squamous lung carcinoma. The antigen may be found at lesion sites or in the blood as indicative of the squamous lung carcinoma.Specific antibodies may be used for the detection of the antigen and in therapy.The mouse hybridoma 43-9F producing IgM monoclonal antibody 43-9F and SLC cell RH-SLC-L11 were deposited at The PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, U.K. on Jan. 31, 1985 and given Accession Nos. 85013101 and 85061403, respectively.
    Type: Grant
    Filed: July 5, 1988
    Date of Patent: May 28, 1991
    Inventor: Lennart Olsson
  • Synthetic vaccine peptide epitomes of hepatitis B surface antigen
    Patent number: 5017558
    Abstract: Novel oligopeptides are provided having serologic activity for the a determinant of hepatitis B virus surface antigen. Included in the oligopeptide chain are a sequence of at least two cysteines and a lysine in proximity to the cysteines. The oligopeptides can find use in immunoassays, the formulation of vaccines, and the production of antisera.
    Type: Grant
    Filed: September 13, 1983
    Date of Patent: May 21, 1991
    Assignee: The Regents of the University of California
    Inventor: Girish N. Vyas
  • Schirmer tear test
    Patent number: 5006310
    Abstract: An improved Schirmer I Tear Test is provided. The improvement comprises a strip having a graduated migration scale printed on the test strip and the presence of a water soluble marker dye that comigrates with the tear migration front. Both improvements serve to increase the reproducibility of the test and ease which with the results are read.
    Type: Grant
    Filed: November 3, 1989
    Date of Patent: April 9, 1991
    Assignee: Visionex
    Inventors: Jerry Gin, Vernon G. Wong
  • Specific ligands for estrogen and progestagen steroid hormone receptors, application and intermediate synthesis products
    Patent number: 5002753
    Abstract: The present invention relates to specific ligands for estrogen or progestagen steroid hormone receptors which have the formula ##STR1## in which X denotes a vinyl group substituted by a radioactive or nonradioactive halogen on the double bond according to a Z isomerism, andY denotes either a hydroxyl group, in which case the ring to which it is attached is an aromatic ring, or a ketone functional group, in which case it is conjugated with a double bond at C.sub.4 -C.sub.5.An application of the ligands according to the invention is the targeted therapy and/or medical imagery, especially of cancer. However, these ligands can also be employed in the quantitative determination of said hormone receptors.However, the labeled ligands according to the invention are of very particular interest in the case of the targeted radiotherapy of cancer.
    Type: Grant
    Filed: October 18, 1989
    Date of Patent: March 26, 1991
    Assignee: IRE-Celltarg S.A.
    Inventors: Marc Zeicher, Jacques Quivy
  • Polypeptide pertussis toxin vaccine
    Patent number: 5000952
    Abstract: A novel method of defining oligopeptides is provided for determining useful immunodominant sequences for use as vaccines for pathogens. The method involves identifying sequences by particular selection procedures and using such sequences with antigen-presenting cells and T-cells to demonstrate activation of the common histocompatibility antigens DQ and DR in humans and their analogs in other animals. The oligopeptides may then be used individually or in combination to produce safe and effective vaccines, where genes may be prepared encoding the oligopeptides and used for expression of the oligopeptides or combinations of the oligopeptides or the gene transformed into the appropriate host, e.g., B. pertussis, for use as a vaccine to the intact organism.
    Type: Grant
    Filed: August 2, 1988
    Date of Patent: March 19, 1991
    Assignee: The Board of Trustees of the Leland Stanford, Jr. University
    Inventors: Lawrence Steinman, Jorge R. Oksenberg, Gary K. Schoolnik, Amrit K. Judd
  • L-ascorbic acid production in microorganisms
    Patent number: 5001059
    Abstract: Improved production of ascorbic acid is obtained empolying Chlorella as a microorganism source and growing the culture under a controlled pattern of carbon source supply. Greatly improved ratios of ascorbic acid to total carbon supplied as well as enhanced ascorbic acid concentrations in the fermentor are obtained.C. pyrenoidosa UV101-158 was deposited at the A.T.C.C. on June 27, 1985 and given Accession No. 53170.
    Type: Grant
    Filed: July 1, 1985
    Date of Patent: March 19, 1991
    Assignee: Bio-Technical Resources, Inc.
    Inventors: Thomas J. Skatrud, Ronald J. Huss