Abstract: The invention relates to increasing the yield of succinate in bacteria by increasing the intracellular availability of cofactors such as NADH.

Abstract: An integrated semiconductor chemical microreactor for real-time polymerase chain reaction (PCR) monitoring, has a monolithic body of semiconductor material; a number of buried channels formed in the monolithic body; an inlet trench and an outlet trench for each buried channel; and a monitoring trench for each buried channel, extending between the inlet and outlet trenches thereof from the top surface of the monolithic body to the respective buried channel. Real-time PCR monitoring is carried out by channeling light beams into the buried channels, possibly through one of the inlet or outlet trenches, whereby the light beams impinge on the fluid therein and collecting the emergent light coming out from the monitoring trench.

Abstract: Methods of increasing the cellular pool of A-CoA and thus driving the metabolic pathways in the direction of A-CoA containing metabolites by overexpressing rate limiting enzymes in A-CoA synthesis. Methods of increasing intracellular levels of CoA and A-CoA through genetic engineering of bacterial strains in conjunction with supplementation with precursor molecules.

Abstract: An apparatus and method to internally provide apertures inside PVC, HDPE, or plastic pipe-riser (blank casing) in existing methane gas recovery wells (extraction wells) that have been installed at Municipal Solid Waste Facilities are described. Apertures in methane well risers allow methane gas, LFG derived from the decomposition of waste, to enter the existing riser and extraction system. This process saves time and cost associated with drilling additional wells to retrieve methane gas from subsequent layers of the waste body. The process assists in maintaining regulatory compliance by capturing LFG and preventing it from being emitted into the atmosphere.