Abstract: A surface energy gradient on a fluid-impervious surface and method of its creation comprising the steps of a) Exposing a base surface having a proximal and a distal portion to a first solution comprising at least one molecule of the formula X-J-M1 wherein X and M1 represent separate functional groups and J represents a spacer moiety that, together, are able to promote formation from solution of a self-assembled monolayer for sufficient time to form a monolayer surface having a uniform surface energy on the base surface. b) Removing a portion of the monolayer of (a) such that a portion of the base surface is again fully or partially exposed. (c) Exposing the portion of the base surface from (b) to at least one other molecule including a functional group having a different surface energy from that of the functional group removed in(b) such that a surface energy gradient from a proximal location to a distal location is formed.