Abstract: Enhanced sensitivity in fluorescent assays employing NADH or NADPH is obtained, particularly in icteric samples, by employing excitation light at a wave length range below about 400 nm and reading emitted light above about 500 nm.

Abstract: Tricyclic antidepressant functionalized compounds are provided for conjugation through a side chain to antigenic compounds, particularly poly(amino acids), and enzymes. The antigenic conjugates are employed for the production of antibodies and together with the enzyme conjugates find particular use in immunoassays for the determination or detection of the total amount of tricyclic antidepressants present in a sample.

Abstract: The method disclosed herein allows for the pretreatment of organisms, suspected of being a material of interest, prior to performing an assay for a determination thereof. The method comprises contacting the organism in an aqueous medium with a composition comprising (1) an enzyme capable of hydrolyzing bonds between N-acetylglucosamine and N-acetylmuramic acid and (2) a chelating agent, in amounts and under conditions sufficient to produce a homogeneous suspension of the organism of interest in the aqueous medium but insufficient to produce lysed cells or spheroplasts. The method and composition are particularly applicable to the pretreatment of cells of gram negative bacteria such as, for example, N. gonorrhoeae, and of chlamydia.

Abstract: Chloramphenicol derivatives are provided for use in the preparing of antigen conjugates for the production of antibodies specifically for chloramphenicol. Specifically, the aryl amino group is derivatized to introduce a non-oxo-carbonyl group which is used for amide formation with an antigen. The conjugate is then injected into a vertebrate for production of antisera which is isolated in conventional ways and find particular use in competitive protein binding assays.

Abstract: Carbonyl derivatives of acetaminophen are provided for use in homogeneous enzyme immunoassays for acetaminophen. The derivatives are conjugated to antigenic substances for the preparation of antisera specific to acetaminophen, and to enzymes for the preparation of enzyme conjugates which compete with acetaminophen for antibody binding sites in a typical assay.

Abstract: Fluorescent antigen conjugates are provided comprising antigens covalantly bonded to at least one 2,7-dialiphatic substituted-9-phenyl-6-hydroxy-3H-xanthen-3-one, wherein the 1- and 8-positions are unsubstituted. Also provided are novel fluorescent compounds absorbing at wavelengths in excess of 500 nm, having active functionalities for linking to the antigen. Finally, methods are provided for analyzing antigens in serum, whereby serum interference is avoided.

Abstract: Novel compositions and methods are provided for detecting the presence of a material of interest in a specimen on a solid surface. A composition useful for detecting the presence of a material of interest in a specimen comprises (1) a detector conjugate comprising a fluorescing moiety bonded to a compound capable of specific binding with the material of interest, or a derivative thereof, and (2) a non-detector conjugate comprising a poly(amino acid) and a compound having substantial structural and charge similarity to the fluorescing compound and no observable fluorescence, or low level fluorescence of a different wavelength than that of the fluoresing compound, in the region of fluorescence of the fluorescing compound. In the method of the invention, a specimen, on a solid surface, is combined with the detector conjugate and the non-detector conjugate, and the combination is incubated.

Abstract: An improved immunoassay for determining the presence of an analyte in a serum sample and conjugates therefor are described. The known method comprises combining the sample with a conjugate of the analyte and an enzyme and with a receptor for analyte, and determining the presence of analyte in the sample from the effect that the sample has on the enzymatic activity when compared to the enzymatic activity in the absence of analyte or in the presence of known amounts of analyte. The present invention resides in the conjugation to the enzyme label of a label protectant which allows modulation of the enzymatic activity to occur and minimizes background interference between the enzyme label and the sample, which would alter the enzymatic activity.

Abstract: Composition and method are provided for enzyme immunoassays for high molecular weight proteins, such as ferritin. An enzyme is conjugated to the high molecular weight protein through a specific linking group. Usually, on the average, about 2 to 10 enzyme molecules are bonded to each protein molecule. In an assay, the conjugate competes with an unknown sample for receptor and the resulting enzymatic activity is compared to a standard for a determination of the presence and amount of protein in the unknown.

Abstract: A method of making-up offset printing cylinders having lock-ups positioned in gaps in the cylinders by use of a two piece system involving adhering a compressible printing element to the cylinder with at least one edge extending into the lock-up gap, trimming the edge extending into the lock-up gap, thereafter pulling a non-compressible printing element carrying the printing ink indicia receptive working surface taut over the compressible printing element and securing the ends of the non-compressible printing element in the lock-up under tension. The two piece system involves separating the relatively thick compressible portion of the offset printing blanket from the relatively thinner ink transfer surface of the offset printing blanket. Also a separate embodiment with pressure sensitive adhesive securing the two piece system together and not limited to offset printing cylinders, product and method.

Abstract: An optical inspection system for the inspection of circular workpieces (1) includes an electronic camera (6) having an optical system (21) for focusing an image of a circular workpiece (1) on an electronically active image receiving surface (23), means (16) to move the circular workpiece (1) into a predetermined position in the field of view of the optical system (21) of the camera (6). When a conventional electronic camera is used to inspect circular workpieces it is very difficult to analyse the output signal from the camera since it corresponds to chords taken across the workpiece (1). To overcome this the present inspection system includes means (7) for scanning the image receiving surface in directions extending substantially radially across the image receiving surface to produce an output signal, and a signal analyser (12) arranged to monitor the output signal corresponding to each radial scan to detect the presence of any irregularity in the workpiece.

Abstract: A container sealing composition made from a plastisol having a mass of hollow discrete microspheres incorporated therein. The composition is characterized by having increased shelf life, as manifested in reduced separation of the hollow discrete microspheres from the plastisol, and improved viscosity stability. The container sealing composition also exhibits uniform void volume which is essential for good sealability and seal quality.

Abstract: A polymer composition comprising a urethane having terminal unsaturation and a terminal carboxyl group and which is capable of further free radical polymerization has been synthesized. This polymer can be a liquid or a solid depending on the molecular weight and backbone of the polymer. This polymer as a liquid has been found to be very useful in producing printed circuit boards by photo resist processes.

Abstract: The present invention is directed to a method for treating resin printing plates to prevent or reduce smudging and/or linting using fluorocarbon surface active agent having a perfluoroalkyl group with 4 to 14 carbon atoms. The surface active agent may be anionic, cationic or ampholytic and may be used in an aqueous, alcohol or aqueous-alcohol solution.

Abstract: A method of making-up offset printing cylinders having lock-ups positioned in gaps in the cylinders by use of a two piece system involving adhering a compressible printing element to the cylinder with at least one edge extending into the lock-up gap, trimming the edge extending into the lock-up gap, thereafter pulling a non-compressible printing element carrying the printing ink indicia receptive working surface taut over the compressible printing element and securing the ends of the non-compressible printing element in the lock-up under tension. The two piece system involves separating the relatively thick compressible portion of the offset printing blanket from the relatively thinner ink transfer surface of the offset printing blanket. Also a separate embodiment with pressure sensitive adhesive securing the two piece system together and not limited to offset printing cylinders, product and method.

Abstract: A polymer composition comprising a urethane having terminal unsaturation and a terminal carboxyl group and which is capable of further free radical polymerization has been synthesized. This polymer can be a liquid or a solid depending on the molecular weight and backbone of the polymer. This polymer as a liquid has been found to be very useful in producing printed circuit boards by photo resist processes.

Abstract: A polymer composition comprising a urethane having terminal unsaturation and a terminal carboxyl group and which is capable of further free radical polymerization has been synthesized. This polymer can be a liquid or a solid depending on the molecular weight and backbone of the polymer. This polymer as a liquid has been found to be very useful in producing printed circuit boards by photo resist processes.

Abstract: Printed circuit boards are made by imaging a liquid polymer which has been coated onto the board. The imaging is accomplished while the polymer is wet with the photo tool in a close air gap relationship with the coated board. The uncured polymer which remains liquid is removed after imaging so that the board can be processed by etch resist, plate resist, or solder mask techniques. The cured polymer is removed by stripping with an alkaline solution. The apparatus consists of single station or multi-station equipment to carry out the unit operations of maintaining the board in a set position, coating with a liquid polymer, placing a photo tool in registration with the circuit board blank and in close relationship with the coating, and imaging the liquid polymer via the photo tool to cause areas of the coating to solidify and other areas to remain liquid.

Abstract: A polymer composition comprising a urethane having terminal unsaturation and a terminal carboxyl group and which is capable of further free radical polymerization has been synthesized. This polymer can be a liquid or a solid depending on the molecular weight and backbone of the polymer. This polymer as a liquid has been found to be very useful in producing printed circuit boards by photo resist processes.

Abstract: A method of preparing a letterpress printing plate from a liquid photopolymer using an air knife to etch out the unpolymerized liquid to leave the relief image. A new printing plate substrate is used that has a laminae that is paper and a lamina that is a sealing coat on the paper. The paper is a special paper known as tag stock that is 5 to 20 mils thick, has tensile strengths of at least 30 pounds per inch in width in the press direction and at least 20 pounds per inch in width in the cross press direction, and fibers that are independently colored with a dark color having a good light absorption/reflectivity ratio.