Abstract: The present invention relates to a novel arrangement of denitrification reactors for removal of nitrate compounds in a recirculating aquaculture system. The novel arrangement of an aquaculture system of the present invention includes positioning one or more anaerobic denitrification reactors upstream of aerobic nitrification and degassing processes. One aspect of the present invention includes a flow of aqueous medium from aquatic species rearing tanks towards one or more denitrification reactors. Another aspect of the present invention includes flow of aqueous medium from aquatic species rearing tanks to a solids removing filter or mechanical filtration means for removal of solid waste matter or biomass prior to flow of aqueous medium towards one or more denitrification reactors.

Abstract: Conditioned media compositions having valuable biological activity are obtained from cultures of immune cells from elasmobranch fishes. A method is provided for producing the conditioned media compositions. Conditioned media obtained using epigonal cells from bonnethead sharks (Sphyrna tiburo) and lemon sharks (Negaprion brevirostris) demonstrate strong anti-tumor activities. The conditioned media compositions can be used for treating tumor proliferation.

Abstract: The present invention includes a horizontal foam fractionation protein skimmer for removing organic waste material (dissolved organic compounds) from organic loaded water. The protein skimmer includes a horizontal reaction chamber connected to a foam collection chamber in which protein-loaded foam containing organic waste material is collected. The reaction chamber is comprised of a series of horizontally-directed, nested, concentric tubes for increasing the number and decreasing the size of bubbles to facilitate efficient removal of dissolved organic compounds. Each horizontal tube has either one or several small openings that are offset from the openings on the adjacent tube at a small angle, typically 10°. The openings are formed by either a single slit that runs along the length of each tube, or by a series of holes that runs along the length of each tube. Organic-loaded water is pumped through tubing into the reaction chamber. As water moves through the tubing, gas (e.g.

Abstract: The present invention describes a method for controlling growth of harmful algal blooms generated by the red tide organism, Karenia brevis, and other harmful algal species. The method includes the steps of applying seawater containing low levels of dissolved ozone directly onto or under the surface of water containing harmful algal blooms. Since only low levels of ozone are required for this method to be effective and since the application of ozonated seawater is directed to the bloom itself, release of excess ozone into the atmosphere and/or surrounding water is minimal, which is advantageous since it greatly reduces adverse effects of ozone on the environment, marine life, and human health. The low concentration of ozone utilized in this method is sufficient to destroy the red tide organism, but leave surrounding marine life unharmed.

Abstract: Conditioned media compositions having valuable biological activity are obtained from cultures of immune cells from elasmobranch fishes. Methods are provided for producing the conditioned media compositions. Conditioned media obtained using epigonal cells from bonnethead sharks (Sphyrna tiburo) and lemon sharks (Negaprion brevirostris) demonstrate strong anti-tumor activities. The conditioned media compositions can be used for treating tumor proliferation and in immunosuppressive therapy.

Abstract: The present invention includes a horizontal foam fractionation protein skimmer for removing organic waste material (dissolved organic compounds) from organic loaded water. The protein skimmer includes a horizontal reaction chamber connected to a foam collection chamber in which protein-loaded foam containing organic waste material is collected. The reaction chamber is comprised of a series of horizontally-directed, nested, concentric tubes for increasing the number and decreasing the size of bubbles to facilitate efficient removal of dissolved organic compounds. Each horizontal tube has either one or several small openings that are offset from the openings on the adjacent tube at a small angle, typically 10°. The openings are formed by either a single slit that runs along the length of each tube, or by a series of holes that runs along the length of each tube. Organic-loaded water is pumped through tubing into the reaction chamber. As water moves through the tubing, gas (e.g.