Patents Represented by Attorney, Agent or Law Firm Cheryl H. Agris, Esq.
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Patent number: 5622988Abstract: The present invention relates to the use of compounds of formula I ##STR1## wherein R.sup.1 is independently hydrogen, hydroxy, alkyl with 1 to 6 carbon atoms, acyloxy groups with 1 to 6 carbon atoms, alkyloxy with 1 to 6 carbon atoms or from 1 to 5 sugar moieties; and R.sup.2 is independently hydrogen, or alkyl with 1 to 6 carbon atoms, in the reduction of coagulation time in mammals.Type: GrantFiled: March 16, 1995Date of Patent: April 22, 1997Assignee: Novo Nordisk A/SInventors: Helle Worsaae, Frank W. Rasmussen, Mirella E. Rasmussen
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Patent number: 5622841Abstract: The invention is directed to an isolated nucleic acid construct(s) comprising a nucleic acid sequence encoding an alkaline protease having a molecular weight of about 34 kD and is obtainable from a strain of Bacillus sp. Group I, as well as recombinant vectors and host cells comprising such constructs. Additionally, the invention is directed to a method for obtaining the protease. The invention is also directed to promoter and signal sequences derived from said alkaline protease as well as methods of using the promoter and signal sequences in the expression of heterologous nucleic acid sequences encoding heterologous proteins in bacteria.Type: GrantFiled: June 2, 1995Date of Patent: April 22, 1997Assignees: Novo Nordisk Biotech, Inc., Novo Nordisk A/SInventors: Alan P. Sloma, Helle Outtrup, Claus Dambmann, Dorrit A. Aaslyng
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Patent number: 5622850Abstract: The invention is directed to an isolated nucleic acid construct(s) comprising a nucleic acid sequence encoding an alkaline protease having a molecular weight of about 34 kD and is obtainable from a strain of Bacillus sp. Group I, as well as recombinant vectors and host cells comprising such constructs. Additionally, the invention is directed to a method for obtaining the protease. The invention is also directed to promoter and signal sequences derived from said alkaline protease as well as methods of using the promoter and signal sequences in the expression of heterologous nucleic acid sequences encoding heterologous proteins in bacteria.Type: GrantFiled: June 2, 1995Date of Patent: April 22, 1997Assignees: Novo Nordisk Biotech, Inc., Novo Nordisk A/SInventors: Alan P. Sloma, Helle Outtrup, Claus Dambmann, Dorrit A. Aaslyng
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Patent number: 5621074Abstract: The present invention relates to methods for producing aprotinin and analogs thereof in yeast, synthetic genes encoding such products, expression vectors and transformed yeast cells. The invention further relates to aprotinin analogs, particularly analogs with increased specific inhibitory activity and/or reduced nephrotoxicity compared to native aprotinin, as well as compositions comprising such analogs.Type: GrantFiled: May 18, 1995Date of Patent: April 15, 1997Assignee: Novo Nordisk A/SInventors: Soren E. Bj.o slashed.rn, Kjeld Norris, Viggo Diness, Leif N.o slashed.rskov-Lauritsen, Niels D. Christensen, Claus Bregengaard, Fanny Norris, Lars C. Petersen
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Patent number: 5621089Abstract: The invention is directed to an isolated nucleic acid construct(s) comprising a nucleic acid sequence encoding an alkaline protease having a molecular weight of about 34 kD and is obtainable from a strain of Bacillus sp. Group I, as well as recombinant vectors and host cells comprising such constructs. Additionally, the invention is directed to a method for obtaining the protease. The invention is also directed to promoter and signal sequences derived from said alkaline protease as well as methods of using the promoter and signal sequences in the expression of heterologous nucleic acid sequences encoding heterologous proteins in bacteria.Type: GrantFiled: May 3, 1995Date of Patent: April 15, 1997Assignees: Novo Nordisk Biotech, Inc., Novo Nordisk A/SInventors: Alan P. Sloma, Helle Outtrup, Claus Dambmann, Dorrit A. Aaslyng
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Patent number: 5618696Abstract: The present invention relates to a human Kunitz-type protease inhibitor comprising the following amino acid sequenceAsp Leu Leu Pro Asn Val Cys Ala Phe Pro Met Glu Lys Gly Pro Cys Gln Thr Tyr Met Thr Arg Trp Phe Phe Asn Phe Glu Thr Gly Glu Cys Glu Leu Phe Ala Tyr Gly Gly Cys Gly Gly Asn Ser Asn Asn Phe Leu Arg Lys Glu Lys Cys Glu Lys Phe Cys Lys Phe Thr(SEQ ID NO:1)or a variant thereof with protease inhibitor properties.Type: GrantFiled: February 6, 1995Date of Patent: April 8, 1997Assignee: Novo Nordisk A/SInventors: Fanny Norris, Kjeld Norris, S.o slashed.ren E. Bj.o slashed.rn, Lars C. Petersen, Ole H. Olsen, Donald C. Foster, Cindy A. Sprecher
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Patent number: 5618915Abstract: The present invention relates to methods for producing aprotinin and analogs thereof in yeast, synthetic genes encoding such products, expression vectors and transformed yeast cells. The invention further relates to aprotinin analogs, particularly analogs with increased specific inhibitory activity and/or reduced nephrotoxicity compared to native aprotinin, as well as compositions comprising such analogs.Type: GrantFiled: May 18, 1995Date of Patent: April 8, 1997Assignee: Novo NordiskInventors: Soren E. Bj.o slashed.rn, Kjeld Norris, Viggo Diness, Leif N.o slashed.rskov-Lauritsen, Niels D. Christensen, Claus Bregengaard, Fanny Norris, Lars C. Petersen
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Patent number: 5610033Abstract: In a process for producing proteins with FVIII activity and FVIII derivatives by in vitro culturing of mammalian cells, the culturing is carried out at temperatures below 32.degree. C. and the culturing times used are below 24 hours.Type: GrantFiled: October 11, 1994Date of Patent: March 11, 1997Assignee: Novo Nordisk A/SInventors: Poul B. Rasmussen, Ole Nordfang
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Patent number: 5604129Abstract: The present invention relates to recombinant thermophilic host cells comprising a nucleic acid sequence encoding a heterologous protein, and a method of producing recombinant protein utilizing same. The recombinant hosts of the present invention provide a better morphology in tank fermentations than many known fungal host cells, such as Aspergillus, which morphology results in lower viscosity levels, and therefore improved productivity.Type: GrantFiled: June 1, 1995Date of Patent: February 18, 1997Assignee: Novo Nordisk Biotech, Inc.Inventors: Ejner B. Jensen, Karuppan C. Boominathan
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Patent number: 5602004Abstract: The present invention relates to recombinant thermophilic host cells comprising a nucleic acid sequence encoding a heterologous protein, and a method of producing recombinant protein utilizing same. The recombinant hosts of the present invention provide a better morphology in tank fermentations than many known fungal host cells, such as Aspergillus, which morphology results in lower viscosity levels, and therefore improved productivity.Type: GrantFiled: July 20, 1994Date of Patent: February 11, 1997Assignee: Novo Nordisk Biotech, Inc.Inventors: Ejner B. Jensen, Karuppan C. Boominathan
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Patent number: 5597720Abstract: An alkaline protease has been isolated from Bacillus sp. PD 498 which has a mass of 34 kD by SDS-PAGE, a pH optimum of 9-11, a pI of 9.3, and a temperature optimum of 40.degree.-55.degree. C. The protease has been formulated into detergent additives and detergents and is suitable for washing processes.Type: GrantFiled: October 26, 1994Date of Patent: January 28, 1997Assignee: Novo Nordisk A/SInventors: Helle Outtrup, Claus Dambmann, Dorrit A. Aaslyng
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Patent number: 5596015Abstract: Compounds having formula (3), wherein R.sup.1 is hydrogen, straight or branched alkyl with 1-10 carbon atoms, straight or branched alkenyl with 2-10 carbon atoms, straight or branched alkynyl with 2-10 carbon atoms, benzyl or aryl, optionally mono- or plurisubstituted with straight or branched alkyl with 1-10 carbon atoms, hydroxy, alkoxy, halogen, an amino or a nitro group; and when R.sup.1 is H salts thereof, or R.sup.1 is acyl (R.sup.1 =--COR.sup.2) wherein R.sup.2 is straight or branched alkyl with 1-10 carbon atoms, alkenyl with 2-10 carbon atoms, alkynyl with 2-10 carbon atoms, or aryl, optionally mono- or plurisubstituted with alkyl with 1-10 carbon atoms, hydroxy, alkoxy, halogen, an amino or a nitro group. Compounds of formula (3) have interesting antifungal activities and may, accordingly, be used as active ingredients in fungicidal compositions.Type: GrantFiled: December 2, 1994Date of Patent: January 21, 1997Assignee: Novo Nordisk A/SInventors: Jens Breinholt, Lene Lange
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Patent number: 5591603Abstract: The present invention relates to methods for producing aprotinin and analogs thereof in yeast, synthetic genes encoding such products, expression vectors and transformed yeast cells. The invention further relates to aprotinin analogs, particularly analogs with increased specific inhibitory activity and/or reduced nephrotoxicity compared to native aprotinin, as well as compositions comprising such analogs.Type: GrantFiled: June 23, 1993Date of Patent: January 7, 1997Assignee: Novo Nordisk A/SInventors: Soren E. Bj.o slashed.rn, Kjeld Norris, Viggo Diness, Leif N.o slashed.rskov-Lauritsen, Niels D. Christensen, Claus Bregengaard, Fanny Norris, Lars C. Petersen
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Patent number: 5580560Abstract: This application relates to modified blood coagulation factors, DNA sequences coding for such modified factors and a process for their production.Type: GrantFiled: August 22, 1994Date of Patent: December 3, 1996Assignee: Novo Nordisk A/SInventors: Else M. Nicolaisen, S.o slashed.ren E. Bj.o slashed.rn, Finn C. Wiberg, Richard Woodbury
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Patent number: 5578489Abstract: Removal of hydrophobic esters from fabric comprises the sequential steps of: 1) impregnating the fabric with an aqueous solution of lipase to a liquor pick-up ratio of 50-200%; 2) incubating the impregnated fabric at 15.degree.-70.degree. C. for 1-24 hours; and 3) washing and rinsing to remove fatty acids.Type: GrantFiled: June 1, 1994Date of Patent: November 26, 1996Assignee: Novo Nordisk A/SInventor: Gitte Petersen
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Patent number: 5573948Abstract: The method for processing of potatoes is characterized by the fact that the method is carried out in a potato processing plant, which comprises an oil separator, heating equipment and at least one enzyme reactor and which is modified in such a manner that it can also be used for processing of rape. It is not obvious that such modification is sufficiently small for achieving an economically sound method for processing of rape outside the potato season.Type: GrantFiled: July 7, 1994Date of Patent: November 12, 1996Assignee: Novo Nordisk A/SInventors: Hans S. Olsen, Erik C. Wormslev
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Patent number: 5569671Abstract: Novel compounds of formula I ##STR1## wherein R.sup.1 is independently hydrogen, hydroxy, alkyl with 1 to 6 carbon atoms, acyloxy groups with 1 to 6 carbon atoms, alkyloxy with 1 to 6 carbon atoms or from 1 to 5 sugar moieties; and R.sup.2 is independently hydrogen, or alkyl with 1 to 6 carbon atoms.The compounds are producible by aerobic cultivation on suitable nutrient media under suitable conditions of a strain of the fungus Ascochyta sp., subsequent recovery of the active component from the fermentation medium, and optionally modifying the active compound to obtain a compound of the desired formula I.Disclosed is also microorganisms capable of producing said compounds, compositions containing said compounds, and the use of such compositions for controlling fungi in valuable crops, animals, including birds and mammals, and in the preservation of wood, paints and edible products.Type: GrantFiled: April 24, 1995Date of Patent: October 29, 1996Assignee: Novo Nordisk A/SInventors: Ruby I. Nielsen, Frank W. Rasmussen
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Patent number: 5556784Abstract: The invention relates to a Bacillus thuringiensis strain(s) which solely produces a CryIA(a) crystal delta-endotoxin having a molecular weight of 130,000 daltons and is active against lepidopteran pests. The invention is also related to a spore(s), mutant(s), or crystal delta-endotoxin obtainable therefrom. Furthermore, the invention relates to insecticidal compositions comprising the B.t. strain, spore, mutant or crystal delta-endotoxin of the present invention. The invention further relates to methods of using the insecticidal compositions to control an insect pest(s) of the order Lepidoptera.Type: GrantFiled: November 23, 1993Date of Patent: September 17, 1996Assignee: Novo Nordisk Entotech, Inc.Inventor: Chi-Li Liu
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Patent number: 5547696Abstract: A pharmaceutical formulation comprising a growth hormone and valine as additive or buffering substance shows a very high stability against deamidation, oxidation and cleavage of peptide bonds. The stability of the product allows for the storing and shipment thereof in a lyophilized state or in the form of a dissolved or re-dissolved formulation at ambient temperature.Type: GrantFiled: October 13, 1994Date of Patent: August 20, 1996Assignee: Novo Nordisk A/SInventor: Hans H. S.o slashed.rensen
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Patent number: 5543502Abstract: A coagulation active complex of Factor VIII fragments is produced by causing a coagulation inactive FVIII heavy chain to react with a coagulation inactive FVIII light chain in the presence of a complex forming agent. Thus, FVIII-HC and FVIII-LC are converted to coagulation active FVIII complex in the presence of metal ions, such as Mn.sup.2+, Ca.sup.2+, or Co.sup.2+ or a component of the prothrombin complex or a substance having reactivity to compounds containing the group --SH and/or --S--S.Type: GrantFiled: February 3, 1995Date of Patent: August 6, 1996Assignee: Novo Nordisk A/SInventors: Ole Nordfang, Mirella E. Rasmussen