Abstract: An immobilized enzyme is prepared for synthesis in a mainly organic medium devoid of free water. In one embodiment, an enzyme and liquid binder are introduced by atomization onto a particulate silica carrier having a particle size below 100 .mu.m in a granulator and simultaneously granulating to form the immobilized enzyme. In another embodiment, a liquid enzyme composition is contacted with the particulate silica carrier to obtain a particulate immobilized enzyme having a particle size below 100 .mu.m, the immobilized enzyme is introduced into granulator, a liquid binder is introduced into the granulator and granulation is carried out. The enzyme is preferably a lipase such as a thermostable lipase, and the immobilized lipase is used for interesterification of fats or synthesis of fatty acid esters.

Abstract: This invention relates to stabilization of phenylalanine ammonia lyase against proteolytic degradation by chemical modification with crosslinking agents, or by genetic modification, a phenylalanine ammonia lyase variant, a method of preparing the variant and a pharmaceutical composition containing phenylalanine ammonia lyase.