Abstract: Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein.
Type:
Grant
Filed:
April 30, 2007
Date of Patent:
June 26, 2012
Assignee:
Butamax(TM) Advanced Biofuels LLC
Inventors:
Andrew C. Eliot, Vasantha Nagarajan, Lixuan Lisa Huang
Abstract: From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of Achromobacter xylosoxidans.
Type:
Grant
Filed:
April 27, 2009
Date of Patent:
May 29, 2012
Assignee:
Butamax(TM) Advanced Biofuels LLC
Inventors:
Michael G. Bramucci, Andrew C. Eliot, Lori Ann Maggio-Hall, Charles E. Nakamura
Abstract: Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
Type:
Grant
Filed:
August 8, 2011
Date of Patent:
May 15, 2012
Assignee:
Butamax(TM) Advanced Biofuels LLC
Inventors:
Gail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
Abstract: Methods for the fermentative production of isobutanol is provided by the fermentative growth of a recombinant microorganism expressing a highly active ketol-acid reductoisomerase enzyme in addition to other enzymes required for conversion of glucose to isobutanol.
Type:
Grant
Filed:
April 16, 2008
Date of Patent:
March 22, 2011
Assignee:
Butamax(TM) Advanced Biofuels LLC
Inventors:
Der-Ing Liao, Mark J. Nelson, Michael G. Bramucci