Abstract: Host cells comprising recombinant vectors encoding the FK-520 polyketide synthase and FK-520 modification enzymes can be used to produce the FK-520 polyketide. Recombinant DNA constructs comprising one or more FK-520 polyketide synthase domains, modules, open reading frames, and variants thereof can be used to produce recombinant polyketide synthases and a variety of different polyketides with application as pharmaceutical and veterinary products.
Type:
Grant
Filed:
October 1, 1999
Date of Patent:
January 7, 2003
Assignee:
Kosan Biosciences, Inc.
Inventors:
Christopher Reeves, Daniel Chu, Chaitan Khosla, Daniel Santi, Kai Wu
Abstract: Compounds of the formula
wherein
Ra is H; substituted or unsubstituted alkyl (1-10C); substituted or unsubstituted alkenyl (2-10C); substituted or unsubstituted alkynyl (2-10C); substituted or unsubstituted aryl (4-14C); substituted or unsubstituted arylalkyl (5-20); or ORa is replaced by H;
Rb is H or halogen;
Rc is H or a protecting group;
Rd is methyl, unsubstituted alkyl (3-10C); substituted alkyl (1-10C); substituted or unsubstituted alkynyl (2-10C); substituted or unsubstituted aryl (4-14C); substituted or unsubstituted arylalkyl (5-20C); substituted or unsubstituted arylalkenyl (5-20C); substituted or unsubstituted arylalkynyl (5-20C); substituted or unsubstituted amidoarylalkyl (5-20C); substituted or unsubstituted amidoarylalkenyl (5-20C); or substituted or unsubstituted amidoarylalkynyl (5-20C);
Re is H or a protecting group;
L is methylene or carbonyl;
T is —O—, —N(R)—, —N(OR)—, —N(NHCOR)—, —N(N═CHR)—, or &mda