Abstract: A purified novel cellulase composition is provided which may be isolated from a fermentation culture of Trichoderma longibrachiatum and has a molecular weight of about 95-105 kD as approximated on SDS-PAGE (see FIG. 1), a pl of about 5.6-6.8 as estimated on an IEF gel and a pH optimum of about 5.0 on RBB-CMC when measured at 65.degree. C. and pH 4 or lower at temperatures of 40.degree. C. and 50.degree. C.
Type:
Grant
Filed:
October 9, 1996
Date of Patent:
January 25, 2000
Assignee:
Genencor International, Inc.
Inventors:
Benjamin S. Bower, Kathleen A. Clarkson, Katherine D. Collier, James T. Kellis, Moira B. Kelly, Edmund A. Larenas
Abstract: Novel .alpha.-amylase enzymes are disclosed in which one or more disulfide bonds are introduced into the enzyme via addition or substitution of a residue with a cysteine. The disclosed .alpha.-amylase enzymes show altered or improved stability and/or activity profiles.