Patent number: 6500405
Abstract: This invention relates to methods for screening compounds for antitubulin activity, evaluation of cells for sensitivity to antitubulin drugs, resistance monitoring and quantifying tubulin using certain amides that inhibit the growth of eukaryotic cells as probes in binding assays, said amides having the structural formula
wherein
A is cycloalkyl, alkyl, haloalkyl, alkylthio, haloalkylthio, alkenyl, haloalkenyl, alkynyl, haloalkynyl, alkoxy, haloalkoxy, or phenyl, pyridyl, furyl, thienyl, isoxazolyl, oxazolyl, pyrrolyl, isothiazolyl, thiazolyl, pyrazolyl, imidazolyl, pyrimidinyl, quinolyl, isoquinolyl, naphthyl, pyridazinyl, pyrazinyl, benzothienyl, indolyl, benzofuranyl or benzyl, all optionally substituted in an acceptable manner with up to 4 substituents;
R1 and R2 are each independently a hydrogen atom, alkyl, haloalkyl, alkenyl, haloalkenyl, alkynyl or haloalkynyl, provided that both R1 and R2 are not a hydrogen atom; and
X, Y and Z are each independently a hydrogen atom, halo, cyano,
Type:
Grant
Filed:
November 4, 1999
Date of Patent:
December 31, 2002
Assignee:
Dow AgroSciences LLC
Inventor:
David Hamilton Young