Abstract: Novel bacteria strains and plants are described which produce and secrete chitinase and other proteins as the result of the introduction of foreign DNA linked to a sequence encoding chitinase, an enzyme capable of degrading chitin present in fungi and nematodes. The bacterial strains have utility in producing chitinase for the purpose of inhibiting plant pathogens. Novel pathogen resistant plants are also described which are created by introduction of DNA encoding for the production of chitinase.

Abstract: A cutter blade assembly for cutting a product, such as a carrot, into a plurality of finished end products, such as carrot sticks, comprises a blade holder and a first and second blade group disposed within the blade holder. The blade holder has a longitudinal passage formed therein. The first blade group comprises a plurality of first blades radially disposed about the longitudinal passage for cutting a first group of finished end products from the product as the product is advanced through the longitudinal passage. The second blade group comprises a plurality of second blades radially disposed about the longitudinal passage and angularly displaced with respect to the first blades. The second blades cut a second group of finished end products from the product as the product is further advanced through the longitudinal passage. Since both the first and second blades produce finished end products, the utilization of the product is maximized, and the amount of waste generated from the product is minimized.

Abstract: Novel bacteria strains and plants are described which produce and secrete chitinase and other proteins as the result of the introduction of foreign DNA linked to a sequence encoding chitinase, an enzyme capable of degrading chitin present in fungi and nematodes. The bacterial strains have utThis invention was made with Government support under Grant No. 1S1-8560311 awarded by the National Science Foundation. The Government has certain rights in this invention.

Abstract: A novel method of producing a plant with a marker closely linked to a target locus, in particular a nuclear male sterile target locus, is described. The method involves transformation of a group of plants in order to introduce a marker into each plant, and isolation of a plant with the marker closely linked to a target locus. The markers include visible markers and dominant conditional lethal markers. The method is of particular use for hybrid seed production where the target locus is a nuclear male sterile locus.

Abstract: This invention relates to the discovery that the prokaryotic enzyme, aminoglycoside 3"-adenyltransferase (AGAT), in particular as encoded by a bacterial aadA gene, is useful as a selectable marker for transformed plants. The enzyme conveys resistance to spectinomycin and streptomycin. Such markers are particularly advantageous because they are non-lethal, provide rapid visual identification of transformed cells and permit selection in media containing either spectinomycin or streptomycin. In addition, AGAT may be used as a selectable marker which differentiates by enabling survival on selective media.

Abstract: A novel method of producing a plant with a marker closely linked to a target locus, in particular a nuclear male sterile target locus, is described. The method involves transformation of a group of plants in order to introduce a marker into each plant, and isolation of a plant with the marker closely linked to a target locus. The markers include visible markers and dominant conditional lethal markers. The method is of particular use for hybrid seed production where the target locus is a nuclear male sterile locus.

Abstract: Promoter sequences from the gene from the small subunit of ribulose-1,5-bisphosphate carboxylase are disclosed. Expression cassettes containing a promoter sequence, a linker region, and a 3' fragment are also disclosed. The promotor sequences and expression cassettes are useful for expressing foreign genes to high levels in transformed plants.

Abstract: Novel bacteria strains are decribed which are created by the introduction of DNA encoding for the production of chitinase, an enzyme capable of degrading chitin present in fungi and nematodes. The strains have utility in producing chitinase for the purpose of inhibiting plant pathogens. Novel pathogen-resistant plants are also described which are created by introduction of DNA encoding for the production of chitinase.

Abstract: A novel method for carrying out immunoassays is described. The method involves use of a biological ice nucleating agent as a label. In particular, the method involves use of ice nucleating bacteria, or ice nucleating components derived therefrom, as a label.

Abstract: Novel bacteria strains are described which are created by the introduction of DNA encoding for the production of chitinase, an enzyme capable of degrading chitin present in fungi and nematodes. The strains have utility in producing chitinase for the purpose of inhibiting plant pathogens.