Abstract: Printing inks, which do not require any petroleum-derived component, are prepared from vegetable oils by heat bodying the oils to within a specified viscosity range. The process is easily tailored to give products having viscosity, tackiness, ruboff resistance, and color density properties which meet or exceed industry standards. The inherent light coloration of the vehicles permits formulation into colored inks having substantially reduced pigment levels.

Abstract: Disclosed are DnA sequences which code for antigenic proteins, methods for identifying such DNA sequences, and antigens coded for by such DNA sequences.The first step of the method is to provide a multiplicity of DNA sequences. These sequences are then inserted into DNA expression vectors to form recombinant expression vectors. The expression vectors are inserted into suitable hosts to form transformants which express the DNA sequences. The transformants are then contacted with antibodies directed against Eimeria antigens to identify transformants containing DNA sequences which code for Eimeria antigens. These antigens are then produced from the DNA sequences identified as coding for the antigens. The antigens so produced are contacted with white blood cells which effect a cell-mediated immune response, which white blood cells are sensitized to an antigenic Eimeria protein, to thereby identify DNA sequences which code for antigens that induce a cell-mediated immune response to avian coccidiosis.

Abstract: Monoclonal cell lines producing antibodies to severe citrus tristeza virus have been developed. The antibodies react with crude plant extracts infected with severe isolates, but not with crude plant extracts infected with mild isolaates or healthy plant tissue. This immunological probe has been utilized in enzyme-linked immunosorbent assays as a rapid technique for identifying citrus infected with severe isolates of CTV. Field tests on recently planted trees in a commercial citrus grove have verified the effectiveness of the immunoassay.

Abstract: A distinctive repetitive genomic element of Leptospira interrogans serovar hardjo-type hardjo-bovis permits distinguishing this pathogen type from other commonly found leptospires in North American cattle using single-stranded RNA probes. Plasmids carrying DNA templates for useful probes have been deposited as NRRL Accession Nos. B-18462, B-18463, and B-18464. The probes are sufficiently sensitive to detect hardjo-bovis in as few as 1.times.10.sup.2 cells/ml. The diagnostic capabilities of the probes render them useful not only as herd management tools, but also in epidemiology studies designed to determine the origin and migration of L. interrogans isolates.

Abstract: Stable heterohybridomas which secrete monoclonal antibodies to bovine herpesvirus-1 (BHV-1) are obtained by fusing primed bovine lymphocytes with an immortal cell line, selecting for secretial hybrids, and sequentially re-fusing such hybrids with fresh bovine lymphocytes. Three such heterohybridomas which secrete neutralizing antibody to BHV-1 have been obtained in this manner and have been deposited in the ATCC as Accession Nos. HB9907, HB9908, and HB9909. The neutralizing antibodies are useful in immunological research, pathological diagnosis, and therapeutic disease control. Specifically, they have utility as serologic control regents in assays, and as primary reagents in anti-species, anti-isotype, and anti-idiotypic antibody production.

Abstract: A gene encoding spinach acyl carrier protein-I-Gly.sup.76 (ACP-I) was fused to a gene encoding the F.sub.c -binding portion of staphylococcal protein A. The gene fusion can be expressed in E. coli thereby resulting in the production of an appropriately sized protein possessing ACP activity in acyl ACP synthetase reactions, plant MCT reactions, and plant fatty acid synthesis. This gene fusion, under the control of the .lambda.P.sub.R promoter, expresses high levels of a 42 kDa fusion protein in E. coli. This protein functions as a affinity moiety in purification methods for the isolation of enzymes involved in fatty acid synthetase, acyl ACP synthetase and malonyl-CoA:ACP tranacylase reactions.

Abstract: Soluble dietary fiber-and-maltodextrin-containing products can be prepared by hydrolyzing a cereal flour or a blend of cereal flour and starch with an .alpha.-amylase and recovering the soluble fraction. The recovered material comprises .beta.-glucans and/or pentosans as the dietary fiber. These compositions are colorless and devoid of inherent undesirable flavors and are, therefore, uniquely suitable for use in a variety of foods.

Abstract: A feeding device singulates, orients, and delivers seeds and other comparatively smooth-surfaced particulate material to a desired destination. The device comprises a horizontal rotating drum having an inner surface cut with a spiral groove for orienting ellipsoid-shaped particles and advancing them to the location of pickup orifices within the drum. A vacuum valve alternately subjects the orifices to vacuum and ambient pressure for picking up the individual particles and releasing them to the desired point of delivery.

Abstract: A versatile device is provided for securely gripping the outer surface of a cylindrical object and pulling it against a resistant force comprising a sleeve that fits around the cylindrical object and engages its outer surface with gripping jaws when a pulling force is applied. The extractor enables efficient and economical extraction of soil access tubes without tube damage. The device can be operated either with large power equipment or with a manual jack.

Abstract: Sprayable, starch-based formulations for autoencapsulating biological control agents, such as pathogenic bacteria and viruses, incorporate a sugary material to promote adherence of the encapsulated agent to treated foliage. The autoencapsulated pathogens are characterized by high survivability and are useful in controlling insects and other pest species.

Abstract: Nematodes are controlled in host animals by contacting the animal or its environment with a bacterial toxin produced by strains of Bacillus laterosporus, the toxin being capable of inhibiting nematode egg hatchability and/or larval development.

Abstract: Single-stranded oligonucleotide probes complementary to a signature region of Treponema hyodysenteriae (T. hyo) 16S rRNA are useful in distinguishing this pathogenic species of Treponema from closely related, nonpathogenic species. The probes find practical application in diagnosing swine dysentary from clinical samples, such as fecal material. They are also useful in differentiating T. hyo strains based on RFLP analysis.

Abstract: A strain of Colletotrichum trancatum has been discovered which is selectively pathogenic toward hemp sesbania (Sesbania exaltata). Formulations incorporating propagules of the fungal pathogen are useful for biological control of the hemp sesbania weed, particularly in agriculatural fields.

Abstract: A substantially pure species of avian Interleukin-2 has a molecular weight of about 30 kda. as determined by SDS-polyacrylamide gel electrophoresis. The compound is obtained from avian lymphocytes. It is produced by collecting lymphocytes from an avian donor, growing the lymphocytes in a medium containing a T cell mitogenic agent, and recovering the compound from the medium.

Abstract: Stable heterohybridomas which secrete monoclonal antibodies to bovine herpesvirus-1 (BHV-1) are obtained by fusing primed bovine lymphocytes with an immortal cell line, selecting for secretial hybrids, and sequentially re-fusing such hybrids with fresh bovine lymphocytes. Three such heterohybridomas which secrete neutralizing antibody to BHV-1 have been obtained in this manner and have been deposited in the ATCC as Accession Nos. HB 9907, HB 9908, and HB 9909. The neutralizing antibodies are useful in immunological research, pathological diagnosis, and therapeutic disease control. Specifically, they have utility as serologic control reagents in assays, and as primary reagents in anti-species, anti-isotype, and anti-idiotypic antibody production.

Abstract: Addition of L-leucine ot the fermentation medium of Fusarium mutant NRRL 18365 increases the production of the trichothecenes neosolaniol, 8-propionyl-neosolaniol, and 8-isobutyryl-neosolaniol. These materials are useful as toxins for the production of or investigation of immunotoxins for cancer therapy.

Abstract: Tissue of Taxus brevifolia has been successfully cultured to produce taxol, related alkaloids, and alkaloid precursors. These procedures will provide a supply of chemotherapeutic agents.

Abstract: A novel indole diterpene compound named "nominine" has been isolated from the sclerotia of the fungus, Aspergillus nomius. Nominine is characterized by the structural formula ##STR1## and is effective for controlling Lepidopteran and Coleopteran insect pests.

Abstract: A transgenic avian line, which is resistant to infection by avian leukosis virus (ALV), subgroup A, has been produced. The line was discovered as a chance event occurring in a program of infecting embryonic chickens with a recombinant ALV virus, selecting individuals assayed to be positive for genomic insertion of proviral sequences, and backcrossing to the original line to obtain G1 progeny demonstrating stable inheritance of the inserts. Of at least three proviral inserts defective for ALV production, one was found to be capable of expressing in the cell membrane subgroup A envelope glycoprotein which specifically interferes with infection by subgroup A virus. This insert represents an artificially introduced dominant gene for ALV disease resistance. Through an appropriate breeding program, a line of chickens homozygous for this gene has been obtained.

Abstract: The levels of essential metals in the mammalian system can be precisely controlled by administering these metals in the form of exogenously synthesized coordination complexes of picolinic acid. The complexes are characterized by the following structural formula: ##STR1## wherein M represents the metallic cation and n is equal to the cation's valence.