Abstract: The electrophoresis method of the present invention employs a media which contains uniformly dispersed liposomes of phospholipid, or combinations of phospholipid and neutral lipid, which contain chromogenic materials or dye precursers. After electrophoresis of a test sample, the liposomes are lysed and the chromogen or dye material released. The chromogen or dye can be any signal producing substance including a chromogenic agent, and enzyme, a fluorogenic agent, or a chemiluminescent agent, but a detectable signal occurs only where the staining material is in close proximity to specific enzymes, effectors, analytes, or other color-inducing agents which have migrated through the gel during electrophoresis.
Type:
Grant
Filed:
August 9, 1988
Date of Patent:
April 9, 1991
Assignee:
Abbott Laboratories
Inventors:
Stanley R. Bouma, Lawrence J. Blecka, Philip Miller
Abstract: The diagnosis of schizophrenia through the detection of a phospholipase enzyme is disclosed. In this invention, a test sample from the patient can be combined with a test composition to measure phospholipase enzyme in the test sample. The sample can be read and the result compared with a scale of values found in nonschizophrenic controls.
Abstract: An ultrathin selectively permeable membrane made by glow discharge plasma polymerization of a single siloxane monomer with the deposition of the polymer as a single layer upon a nonporous support material. The resulting membrane has an oxygen content between about 10% and 60% and is highly permselective. The support material can be an electrode, thereby forming an electrode that is resistant to fouling or contamination but readily accessible to oxygen.
Abstract: The present invention relates to methods and materials for the detection of ketone and aldehyde analytes in fluid samples by means of reacting analyte containing samples with a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which an amine is covalently coupled. Methods and devices are also provided for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising determining the breath acetone concentration of the subject.
Abstract: A method of performing a diagnostic immunoassay utilizing colloidal non-metal particles having conjugated thereto a binding component capable of specifically recognizing an analyte to be determined. After reaction of the sample and colloidal non-metal particles, the presence or amount of analyte/colloidal non-metal particle complexes are determined by optical analysis as a measure of the amount of analyte in the sample. The method can be utilized for the specific detection of numerous analytes and is sensitive and has a wide detection range.
Type:
Grant
Filed:
July 9, 1987
Date of Patent:
September 4, 1990
Assignee:
Abbott Laboratories
Inventors:
David A. Yost, John C. Russell, Heechung Yang
Abstract: An antimicrobial composition containing quinolones and parabens provides surprising fungicidal and bactericidal activity when used in serum or protein-based in-vitro diagnostic kits. Also, a method of inhibiting the growth of bacteria and fungi in in-vitro diagnostic kits by adding the new antimicrobial composition to the kit components.
Type:
Grant
Filed:
March 23, 1988
Date of Patent:
June 5, 1990
Assignee:
Abbott Laboratories
Inventors:
Bonnie Berger-Neel, Beth K. Gershon, John P. Groff