Abstract: The present invention relates to novel protein variants that exhibit reduced allergenicity when compared to the parental proteins. Also included are DNA molecules that encode the novel variants, host cells comprising the DNA and methods of making proteins less allergenic.
Type:
Grant
Filed:
May 12, 2005
Date of Patent:
January 13, 2009
Assignees:
Genencor International, Inc., The Procter & Gamble Company
Inventors:
David A. Estell, Grant C. Ganshaw, Fiona A. Harding, Edmund A. Larenas, Ayrookaran J. Poulose, Elizabeth E. Sikorski, Elliott P. Russell
Abstract: The present invention relates to modified pullulanases useful in the starch industry. The present invention provides methods for producing the modified pullulanase, enzymatic compositions comprising the modified pullulanase, and methods for the saccharification of starch comprising the use of the enzymatic compositions.
Abstract: DNA is provided which encodes an enzyme having phytase activity isolated from Penicillium, Fusarium, Humicola and Emericella. Also provided for is a method of isolating DNA encoding an enzyme having phytase activity from organisms which possess such DNA, transformation of the DNA into a suitable host organism, expression of the transformed DNA and the use of the expressed phytase protein in feed as a supplement.
Type:
Grant
Filed:
January 26, 2007
Date of Patent:
November 11, 2008
Assignee:
Genencor International, Inc.
Inventors:
Benjamin Bower, Frits Goedegebuur, Kathleen A. Clarkson, Scott D. Power
Abstract: The present invention is directed to novel acid proteases and more specifically to NSP24 family proteases and NSP25 family proteases including biologically active fragments thereof and to nucleic acid molecules encoding said proteases. Also provided are vectors and host cells including nucleic acid sequences coding for the proteases, methods for producing the proteases, enzyme compositions and methods employing said proteases.
Type:
Grant
Filed:
December 20, 2005
Date of Patent:
September 30, 2008
Assignee:
Genencor International, Inc.
Inventors:
Kathleen A. Clarkson, Nigel Dunn-Coleman, Suzanne E. Lantz, Craig E. Pilgrim, Piet van Solingen, Michael Ward
Abstract: DNA is provided which encodes an enzyme having phytase activity isolated from Penicillium, Fusarium, Humicola and Emericella. Also provided for is a method of isolating DNA encoding an enzyme having phytase activity from organisms which possess such DNA, transformation of the DNA into a suitable host organism, expression of the transformed DNA and the use of the expressed phytase protein in feed as a supplement.
Type:
Grant
Filed:
January 26, 2007
Date of Patent:
September 30, 2008
Assignee:
Genencor International, Inc.
Inventors:
Benjamin Bower, Frits Goedegebuur, Kathleen A. Clarkson, Scott D. Power
Abstract: The present invention generally relates to hyphal growth in fungi and in particular describes the modulation of genes associated with hyphal growth in filamentous fungi. The present invention provides methods and systems for the production of proteins and/or chemicals from filamentous fungi which comprise modulation of genes associated with hyphal growth. Specifically, the present invention is directed to a full length cotA gene, its gene product and methods of use.
Type:
Grant
Filed:
April 25, 2005
Date of Patent:
September 16, 2008
Assignee:
Genencor International, Inc.
Inventors:
Ali R. Akin, Elizabeth A. Bodie, Shirley M. Burrow, Nigel Dunn-Coleman, Geoffrey Turner, Michael Ward
Abstract: The present invention is related to glucoamylases having at least 80% sequence identity to a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.
Type:
Grant
Filed:
August 28, 2006
Date of Patent:
August 19, 2008
Assignee:
Genencor International, Inc.
Inventors:
Nigel Dunn-Coleman, Paulien Neefe-Kruithof, Craig E. Pilgrim, Piet van Solingen, Donald E. Ward
Abstract: The present invention relates to modified pullulanases useful in the starch industry. The present invention provides methods for producing the modified pullulanase, enzymatic compositions comprising the modified pullulanase, and methods for the saccharification of starch comprising the use of the enzymatic compositions.
Abstract: The present invention makes use of unique tags of a specific biopolymer that can be exploited for determining the concentration the biopolymer in crude solutions. In preferred embodiments the biopolymer is either a protein or a polynucleotide. Particularly, the invention provides a method for the determination and quantitation of biomolecules in crude mixtures by way of a separation technique in combination with mass spectroscopy. In one general embodiment, a target biomolecule is selected for analysis and an analog thereof is generated. Peak area integration of the peptide pairs provides a direct measure for the amount of target protein in the crude solution.
Type:
Grant
Filed:
December 14, 2004
Date of Patent:
July 8, 2008
Assignee:
Genencor International, Inc.
Inventors:
David A. Estell, Grant C. Ganshaw, Christian Paech, Sigrid Paech
Abstract: Described herein are methods for the enhanced production of secreted proteins. The secretion of a protein of interest having a substantially non-polar carboxy tail is enhanced by the placement of charged amino acid residues at the carboxy terminus either by adding to the native peptide or by replacing, i.e., substituting, the terminal residues of the native peptide.
Abstract: The present invention relates to isolated promoter sequences, particularly a promoter isolated from Aspergillus niger designated herein as A4-L or A4 and DNA constructs and vectors including the same.
Type:
Grant
Filed:
November 18, 2004
Date of Patent:
May 27, 2008
Assignee:
Genencor International, Inc.
Inventors:
Steve Kim, Edwin Lee, Wei Liu, Huaming Wang