Abstract: A method to make libraries of hybrid polynucleotide molecules of two parental polynucleotide molecules utilizing single-stranded DNA was invented. Example of the method comprises several steps: (i) preparation of two single-stranded polynucleotide molecules comprising sequences containing one or more parts of homology and one or more parts of heterology, (ii) random or non-random fragmentation of said polynucleotides, (iii) hybridization of the fragmented molecules followed by de novo polynucleotide synthesis (i.e. polynucleotide chain elongation) on the hybridized molecules, (iv) separation of the chain elongation products (i.e. double-stranded polynucleotide molecules) into single-stranded polynucleotide molecules (denaturation) (v) hybridization of the resultant single-stranded polynucleotide molecules followed by de novo polynucleotide synthesis on the hybridized molecules, and (vi) repeating at least two further cycles of steps (iv) and (v).

Abstract: Nucleic acids encoding a novel ferritin light chain variant, a ferritin light chain variant polypeptide, ferritin light chain variant polypeptide-specific antibodies, and methods of use thereof are provided herein. Also provided are methods to screen and identify agents capable of modulating the activity of the ferritin light chain variant.

Abstract: Methods are provided for the identification, biochemical characterization and therapeutic use of agents which impact PTEN, p53, PI-kinase and AKT mediated cellular signaling.

Abstract: A gravity-flow grain dryer for particulate material comprises a generally vertical drying column having first and second opposed spaced perforate walls, the column being adapted to receive particulate material and direct the material through the dryer. An input is provided for introducing moist particulate material into a top portion of the column and a discharge mechanism is provided for removing dried particulate material from a bottom portion of the column. A blower and heater are also provided for passing drying air into the column through the first perforate wall and out through the second perforate wall, the air drying the material within the column. A dividing wall extends between the perforate walls for dividing at least a portion of the column into at least two channels, of the channels containing a discharging mechanism for removing particulate material from the channel.