Abstract: A new expression system is provided which comprises component(s) of a lipase regulation cascade. the lipase regulation cascade as disclosed herein includes a kinase, a DNA binding regulator, a polymerase, a promoter, an upstream activating sequence, and secretion factors. Plasmids and transformed cells are also provided as well as methods of transforming host cells using the plasmids. Further, there is provided a kinase that can regulate the expression of a protein, a DNA binding regulator that can regulate the expression of a protein, a Pseudomonas alcaligenes polymerase, a Pseudomonas alcaligenes sigma 54 promoter, a Pseudomonas alcaligenes upstream activating sequence, the Pseudomonas alcaligenes secretion factors XcpP, XcpQ, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and the xcp regulators OrfV, OrfX.

Abstract: There are provided novel transposable elements isolated from Aspergillus. Also provided are novel fragments comprising the inverted repeat(s) of the transposable elements, such fragments being useful as probes to isolate transposable elements from other filamentous fungi.

Abstract: An immobilized enzyme preparation is disclosed which comprises a gelling agent which has been cross-linked and active carbon in an amount of 1 to 3%. The gelling agent may be egg white chitosan or alginate. The enzyme glucose isomerase may be employed. When used in packed bed columns in enzymatic glucose isomerization processes, the disclosed immobilized enzyme formulation allows for a bedheight that is twice as high, a syrup flow that is doubled, or a doubled syrup viscosity when compared to formulations without carbon.

Abstract: The invention relates to a novel water-insoluble glucose isomerase which is formed by a crystalline enzyme converted to solid form by cross-linking. The invention also concerns a process for the preparation of the novel crystalline glucose isomerase by cross-linking with dialdehyde in the presence of a compound containing at least one amino group, and the use of this novel enzyme preparation as an isomerization catalyst.

Abstract: This invention relates to .alpha. and .beta.-L-pyranosyl nucleosides of Formula (I), wherein the nucleoside substitution on the pyranosyl carbohydrate molecule comprises a substituted or unsubstituted purine (adenine or guanine) or pyrimidine (cytosine, uracil, thymine and hypoxanthine) base. Also provided are methods to make the .alpha. and .beta.-L-pyranosyl nucleosides and methods of using such to treat cancer in a mammal.

Abstract: This invention relates to novel mutant filamentous fungi which are deficient in the gene for the corresponding aspartic proteinase. These organisms are useful production hosts in the production of heterologous polypeptides such as chymosin.

Abstract: The present invention relates to methods of enriching for desired cell population from cell sources, such as body fluids, dispersed tissue specimens and cultured cells. In particular, the present invention relates to the use of a cell-trap centrifugation tube containing a specific density gradient solution adjusted to the specific density of a desired cell population to enrich for the desired cell from a cell source. The tube allows the desired cell population to be collected by decantation after centrifugation to minimize cell loss and maximize efficiency. In addition, the method can be further simplified by density-adjusted cell sorting which uses cell type-specific binding agents such as antibodies and lectins linked to carrier particles to impart a different density to the undesired populations in a more convenient manner. The rapid cell enrichment method described herein has a wide range of diagnostic and therapeutic applications.

Abstract: The invention relates to a novel water-insoluble glucose isomerase which is formed by a crystalline enzyme converted to solid form by cross-linking. The invention also concerns a process for the preparation of the novel crystalline glucose isomerase by cross-linking with dialdehyde in the presence of a compound containing at least one amino group, and the use of this novel enzyme preparation as an isomerization catalyst.

Abstract: According to the invention a method is provided for liquefying starch comprising the steps of treating the starch prior to or simultaneously with liquefying the starch to inactivate and/or remove the enzyme inhibiting composition present in the starch and form treated starch; adding .alpha.-amylase to the treated starch; and reacting the treated starch for a time and at a temperature effective to liquefy the treated starch. Effective means to treat the starch include the addition of a phytate degrading enzyme and heat treatment, optionally followed by filtration or centrifugation, of granular starch or a starch solution.

Abstract: Novel methods are disclosed for the enhanced expression and secretion of lactase from filamentous fungi. Specifically the novel processes cause enhanced production of lactase from an Aspergillus and preferably enhanced production of A. oryzae lactase from A. oryzae host strains transformed with necessary DNA. Also described are the DNA sequence encoding the lactase gene from A. oryzae and the deduced amino acid sequence of the lactase therefrom.

Abstract: The present invention relates to methods of enriching fetal cells from maternal body fluids. In particular, it relates to the use of a cell-trap centrifugation tube containing a gradient solution adjusted to a specific density to enrich for fetal nucleated red blood cells from maternal blood. The tube allows the desired cell population to be collected by decantation after centrifugation to minimize cell loss and maximize efficiency. In addition, the method can be further simplified by density-adjusted cell sorting which uses cell type-specific binding agents such as antibodies and lectins linked to carrier particles to impart a different density to undesired cell populations allowing the fetal cells to be separated during centrifugation in a more convenient manner. The rapid fetal cell enrichment method described herein has a wide range of applications, including but not limited to, gender determination and prenatal diagnosis of genetic diseases without the use of invasive procedures.