Abstract: Genes encoding Class II EPSPS enzymes are disclosed. The genes are useful in producing transformed bacteria and plants which are tolerant to glyphosate herbicide. Class II EPSPS genes share little homology with known, Class I EPSPS genes, and do not hybridize to probes from Class I EPSPS's. The Class II EPSPS enzymes are characterized by being more kinetically efficient than Class I EPSPS's in the presence of glyphosate. Plants transformed with Class II EPSPS genes are also disclosed as well as a method for selectively controlling weeds in a planted transgenic crop field.
Type:
Grant
Filed:
September 13, 1994
Date of Patent:
May 27, 1997
Assignee:
Monsanto Company
Inventors:
Gerard F. Barry, Ganesh M. Kishore, Stephen R. Padgette, William C. Stallings
Abstract: Genes encoding Class II EPSPS enzymes are disclosed. The genes are useful in producing transformed bacteria and plants which are tolerant to glyphosate herbicide. Class II EPSPS genes share little homology with known, Class I EPSPS genes, and do not hybridize to probes from Class I EPSPS's. The Class II EPSPS enzymes are characterized by being more kinetically efficient than Class I EPSPS's in the presence of glyphosate. Plants transformed with Class II EPSPS genes are also disclosed as well as a method for selectively controlling weeds in a planted transgenic crop field.
Type:
Grant
Filed:
June 7, 1995
Date of Patent:
May 6, 1997
Assignee:
Monsanto Company
Inventors:
Gerard F. Barry, Ganesh M. Kishore, Stephen R. Padgette, William C. Stallings
Abstract: Promoters for enhanced expression of ADPglucose pyrophosphorylase in potato tubers and fruits such as tomato; methods of using them; DNA molecules, plant cells and plants containing them. A method of decreasing the oil content of seeds by expression of ADPglucose pyrophosphorylase.
Type:
Grant
Filed:
March 3, 1995
Date of Patent:
March 4, 1997
Assignee:
Monsanto Company
Inventors:
Gerard F. Barry, Ganesh M. Kishore, David M. Stark
Abstract: An isolated DNA sequence which codes for a potyvirus protease gene is disclosed herein. A method for providing resistance to infection by a virus by expressing a protease gene in plants is also disclosed. Transgenic potato plants and tubers containing the protease gene are also disclosed.
Abstract: A plant promoter that is a nucleic acid region located upstream of the 5' end of a plant DNA structural coding sequence that is transcribed at high levels in meristematic tissue and/or rapidly dividing cells. This promoter region is capable of conferring high levels of transcription in meristematic tissue and/or rapidly dividing cells when used as a promoter for a heterologous coding sequence in a chimetic gene. The promoter and any chimeric gene in which it may be used can be used to obtain transformed plants or plant cells. A DNA coding sequence that codes for a gene that is highly transcribed in meristematic tissue of Arabidopsis thaliana is also disclosed. This coding sequence can be used to obtain a cDNA probe useful in obtaining analogous promoters from a homologous coding sequence in other plant species. Chimeric genes including the isolated promoter region, transformed plants containing the isolated promoter region, transformed plant cells and seeds are also disclosed.
Abstract: A method for Agrobacterium-mediated transformation and regeneration of soybean is disclosed. The method utilizes a cotyledon explant which is prepared by first removing the hypocotyl and then tearing the two cotyledons apart at the cotyledonary node. The explant may be inoculated with either a smear of the disarmed Agrobacterium vector or of liquid culture of the bacterium.
Abstract: 3-Hydroxysteroid oxidase controls insects, particularly lepidopteran and boll weevil. Genes encoding for this enzyme may be cloned into vectors for transformation of plant-colonizing microorganisms, thereby providing a method of controlling insect infestation.
Type:
Grant
Filed:
June 7, 1995
Date of Patent:
September 24, 1996
Assignee:
Monsanto Company
Inventors:
David R. Corbin, John T. Greenplate, Michael G. Jennings, John P. Purcell, Robert D. Sammons
Abstract: Disclosed are certain 3-phenoxypyridazines, compositions thereof which are herbicidal and methods of using such compositions for controlling undesired plants. Intermediate compounds useful in preparing the phenoxypyridazines are also disclosed.
Abstract: 3-Hydroxysteroid oxidase controls insects, particularly lepidopterans and boll weevil. Genes encoding for this enzyme may be cloned into vectors for transformation of plant-colonizing microorganisms or plants, thereby providing a method of controlling insect infestation.
Type:
Grant
Filed:
February 24, 1995
Date of Patent:
September 10, 1996
Assignee:
Monsanto Company
Inventors:
David R. Corbin, John T. Greenplate, Michael G. Jennings, John P. Purcell, Robert D. Sammons
Abstract: Herbicidal compositions of glyphosate, its salts, diquat, or paraquat in combination with a compound of the following formula ##STR1## wherein R.sub.1, R.sub.2, R.sub.3, R.sub.4, and n are as defined. Preferred are combinations of glyphosate, its salts, diquat, or paraquat with 2-chloro-5-(4-bromo-1-methyl-5-(trifluoromethyl)-1H-pyrazol-3-yl)-4-fluoro benzoic acid, 1-methylethyl ester.
Type:
Grant
Filed:
December 20, 1993
Date of Patent:
July 16, 1996
Assignee:
Monsanto Company
Inventors:
Scott S. Woodard, Bruce C. Hamper, Kurt Moedritzer, Michael D. Rogers, Deborah A. Mischke, Gerard A. Dutra
Abstract: Disclosed are certain 3-pyrazolyloxypyridazines, compositions thereof which are herbicidal and methods of using such composition for controlling undesired plants. Also disclosed are mixtures of such pyridazines and acetanilide herbicides, to which mixture a safener may be added, if desired. Intermediate compounds useful in preparing the pyrazolyloxypyridazines are also disclosed.
Abstract: The present invention relates to novel 4-halo-2-fluoro-5-alkylbenzoyl compounds and their methods of manufacture. These compounds are useful for the preparation of agricultural chemicals and medicines, particularly as intermediates for an active class of aryl-haloalkylpyrazole and aryl alkylsulfonylpyrazole herbicides.
Type:
Grant
Filed:
July 20, 1994
Date of Patent:
July 2, 1996
Assignee:
Monsanto Company
Inventors:
Bruce C. Hamper, Kindrick L. Leschinsky
Abstract: In one aspect the present invention relates to the use of viral promoters in the expression of chimeric genes in plant cells. In another aspect this invention relates to chimeric genes which are capable of being expressed in plant cells, which utilize promoter regions derived from viruses which are capable of infecting plant cells. One such virus comprises the cauliflower mosaic virux (CaMV). Two different promoter regions have been derived from the CaMV genome and ligated to heterologous coding sequences to form chimeric genes. These chimeric genes have been shown to be expressed in plant cells. This invention also relates to plant cells, plant tissue, and differentiated plants which contain and express the chimeric genes of this invention.
Type:
Grant
Filed:
September 2, 1994
Date of Patent:
June 25, 1996
Assignee:
Monsanto Company
Inventors:
Robert T. Fraley, Robert B. Horsch, Stephen G. Rogers
Abstract: The disclosure herein relates to a process for the preparation of compounds according to Formula N ##STR1## which comprises using a compound of Formula M ##STR2## as starting material for alkylation to said compounds of Formula N in an inert solvent at temperatures within the range of 0.degree.-200.degree. C. whereinR.sub.1 is C.sub.1-5 alkyl;R.sub.2 is C.sub.1-5 haloalkyl;R.sub.3 is halogen;R.sub.5 is hydrogen or halogen;q is 0-2;R.sub.28 and R.sub.30 are independently an alkyl, alkenyl or alkynyl radical having up to 5 carbon atoms or said radicals substituted with a ##STR3## radical; X is O, S(O).sub.m, NR.sub.19 or CR.sub.20 R.sub.21 ;Y is O, S(O).sub.m or NR.sub.22 ;m is 0-2 andR.sub.13 and R.sub.19 -R.sub.22 are hydrogen or an R.sub.28 member.The above compounds are useful as herbicides.
Type:
Grant
Filed:
January 31, 1994
Date of Patent:
June 25, 1996
Assignee:
Monsanto Company
Inventors:
Scott S. Woodard, Bruce C. Hamper, Kurt Moedritzer, Michael D. Rogers, Deborah A. Mischke, Gerard A. Dutra
Abstract: 3-Hydroxysteroid oxidase controls insects, particularly lepidopterans and boll weevil. Genes encoding for this enzyme may be cloned into vectors for transformation of plant-colonizing microorganisms or plants, thereby providing a method of controlling insect infestation.
Type:
Grant
Filed:
June 28, 1993
Date of Patent:
May 21, 1996
Assignee:
Monsanto Company
Inventors:
David R. Corbin, John T. Greenplate, Michael G. Jennings, John P. Purcell, Robert D. Sammons
Abstract: Plants can be transformed to express glucose oxidase from Aspergillus sp. and be made resistant to bacterial and fungal pathogens. Optionally, the plants may also express invertase to increase the available glucose as a substrate for enzymatic activity.
Type:
Grant
Filed:
November 3, 1994
Date of Patent:
May 14, 1996
Assignee:
Monsanto Company
Inventors:
Ellen B. Lawrence, Elaine B. Levine, Dilip M. Shah
Abstract: A method for controlling the ripening of fruits and vegetables as well as a method for controlling senescence of plant tissue is described. The method generally embraces the expression of an ACC metabolizing enzyme in the fruit or other desired plant tissue to inhibit the production of ethylene in the fruit or plant tissue. The use of the ACC metabolizing enzyme ACC deaminase is described in detail. The ripening or senescence process in the fruit or plant tissue is inhibited by the expression of the ACC deaminase gene such that the shelf-life and marketability of the fruit or plant is enhanced. The ACC metabolizing enzyme may be used in combination with other methods for reducing ethylene production in transformed plants to further reduce the production of ethylene in the fruit or plant. DNA constructs containing the ACC deaminase gene are also described.