Abstract: Methods and reagents are provided for amplifying and detecting target nucleic acid sequences in a sample suspected of containing Borrelia spirochetes using the polymerase chain reaction (PCR) process. The PCR process employs primers and probes which are derived from the 16S ribosomal RNA gene of Borrelia burgdorferi and Borrelia hermsii.
Abstract: The present invention provides improved primers for the polymerase chain reaction (PCR) amplification of a nucleic acid sequence from hepatitis C virus (HCV). The primers and amplification methods of the invention enable the detection of HCV with greatly increased sensitivity.
Abstract: Methods and reagents for determining an individual's genotype at the ABO locus with respect to newly discovered polymorphisms, which facilitates typing tissue for determinign individual identity and has application in the field of forensic science.
Abstract: The presence or absence of a nucleic acid sequence associated with AIDS in a sample containing one or more nucleic acids and suspected of containing such sequence can be detected by amplifying the sequence using primers to form extension products as templates and detecting the amplified product if it is present. This may be accomplished by adding a labeled hybridization probe to the amplified product either free in solution or after immobilization on a solid support. Exemplary primers and probes for amplifying and detecting AIDS virus are provided.
Type:
Grant
Filed:
July 16, 1993
Date of Patent:
January 31, 1995
Assignee:
Hoffman-La Roche Inc.
Inventors:
John J. Sninsky, Shirley Y. Kwok, David Mack, Henry A. Erlich, Kary B. Mullis