Abstract: A method is disclosed for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4-trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4-trifluoromethylcoumarin chromophore in the hydrolyzate.

Abstract: A method is disclosed for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4-trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4-trifluoromethylcoumarin chromophore in the hydrolyzate.

Abstract: Fluorogenic substrates for proteolytic enzymes having the formula: ##STR1## or acid salts thereof wherein: R.sub.1 is hydrogen-L, hydrogen-D, benzoyl, benzenesulfonyl, glutaryl, pyroglutamyl, carbobenzoxy, D-serine, or carbobenzoxy-serine;R.sub.2 is hydrogen, phenyl, a straight, branched or cyclic alkyl having 1 to 4 carbons, or propionic acid;R.sub.3 is hydrogen, straight or branched or cyclic alkyl having 1 to 4 carbons, 4-aminobutane, or 3-guanidylpropane;R.sub.4 is methyl, 4-aminobutane, or 3-guanidylpropane;R.sub.5 is a fluorogenic moiety, severable from said compound by a proteolytic enzyme and having different fluorescent property when severed from said compound than when forming part of said compound.The enzymes, when reacting with the substrate, remove the fluorogenic group R.sub.5 producing an increase in its fluorescence. The increase of the fluorescence is an indication of the activity of the enzyme present. Specific enzymes thusly detectable include plasmin, thrombin, the factors X.sub.a, XI.sub.

Abstract: A low ionic strength suspending medium for immunologic reactions which includes a salt solution, a buffer, gelatin, albumin, and an organic solute to control osmolality.

Abstract: A method of detecting antibodies to native deoxyribonucleic acid (n-DNA), anti-nuclear antibodies, antibodies to non-histone containing protein, rheumatoid factors, antibodies to thyroglobulin, and an immunofluorescent assay for such detection is described.

Abstract: A method of detecting antibodies to native deoxyribonucleic acid (n-DNA), anti-nuclear antibodies, antibodies to non-histone containing protein, rheumatoid factors, antibodies to thyroglobulin, and an immunofluorescent assay for such detection is described.

Abstract: A set of four cell suspensions containing erythrocytes monospecifically sensitized with IgG, IgM, C3, and C4. The suspensions provide control cells to determine the purity and strength of the antibodies to these immunoglobins or complement components contained in antisera used for clinical testing. Strict control of the pH, temperature, and EDTA concentration of a solution to which blood is added allows for the elective production of C3- or C4-sensitized cells. Selecting a serum with IgG or IgM antibodies and inactivating undesired proteins permits the production IgG- or IgM-sensitized cells. The resultant cells have agglutination reaction strengths of about 2-4+. Erythrocytes from humans or other animals may undergo the monospecific sensitization. Other human proteins, such as IgA or C5, and plasma proteins from other animals may similarly be attached monospecifically to red blood cells.

Abstract: A method of detecting antibodies to native deoxyribonucleic acid (n-DNA), anti-nuclear antibodies, antibodies to non-histone containing protein, rheumatoid factors, antibodies to thyroglobulin, and an immunofluorescent assay for such detection is described.

Abstract: Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 4-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes.

Abstract: Disclosed herein is a method for producing maltooligosaccharide glycosides in substantially pure form which includes the steps of incubating a glucosyl donor and a glucosyl acceptor in the presence of a glucanotransferase enzyme under transglycosylating conditions and separating the maltooligosaccharide glycoside from the reaction mixture.

Abstract: A reagent for detection of aldehydes in biological specimens. The reagent is a modified Schiff reagent which is stable at room temperature for a substantial period of time. The reagent comprises potassium metabisulfite, pararosanilin acetate and hydrochloric acid. The potassium metabisulfite and pararosanilin acetate are included in the reagent in equal amounts. The sulfur dioxide source is potassium metabisulfite. The pH of the reagent ranges from about 1.2 to about 2.7.

Abstract: A filter device for connecting in series with a parenteral liquid administration line delivering a medical liquid to a patient. The filter unit includes a housing with a filter member that is previous to liquid but impervious to gas, and a gas collection chamber. A nonpuncturable hypodermic needle guide structure on the unit aligns a hypodermic needle with the gas collection chamber and limits cocking of the needle to prevent puncture or damage to the filter member.

Abstract: Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 5-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes.

Abstract: A snap-on retention ring by which a hospital pharmacist can easily attach an additive cap over a sealed closure of a medical liquid container after the pharmacist has added supplemental medication to the container. The retention ring can be of a bright color as a reminder to a nurse that additive drugs have been applied to the container and special instructions should be read carefully.

Abstract: A method is disclosed for partially resolving the components of a pentaerythritol waste liquor which contains pentaerythritol, polypentaerythritols, organic syrups, water, and an alkali metal formate which comprises acidifying the waste liquor to convert the alkali metal formate to formic acid and an alkali metal salt, adding a lower aliphatic alcohol to the acidified waste liquor to react with the formic acid to form a formate ester and to precipitate the alkali metal salt, removing the formate ester by distillation, and removing the precipitated alkali metal salt.

Abstract: A secondary sampling device for particulate matter is disclosed which includes a plurality of sample splitters arranged in series wherein each sample splitter is perpendicular to those adjacent thereto.

Abstract: Improved explosive slurries of the type containing an oxidizing salt, an organic fuel, a metallic fuel, a thickening agent, and water, wherein the improvement includes a sensitizing amount of an inorganic peroxide.

Abstract: Novel steroid ethers are disclosed, which are represented by the formula: ##STR1## wherein Z is selected from the group consisting of >C=O >CHOH, and ##STR2## X is selected from the group consisting of --H and --OH, and R is selected from the group consisting of ##STR3## wherein R.sub.1 is a straight or branched chain hydrocarbon of from 1 to about 16 carbon atoms; A is selected from the group consisting of --CH.sub.2 --, >C=O, and >CHOR.sub.3 ; Y may be a single bond or a double bond; R.sub.2 and --R.sub.1 O-- represent substituents on the aromatic ring of the group of the second formula, in either the 2 or 4 positions, and R.sub.2 is selected from the group consisting of H and OR.sub.3 ; R.sub.