Abstract: Retroviruses are used as genetic tools to isolate transcriptionally active chromosomal regions. The retroviruses have a promoterless protein coding sequence located in U3 or U5. The retroviruses may be used to infect cells under conditions which permit selection for instances when the retrovirus integrates in close proximity to and under the control of a cellular promoter. The promoter and its associated gene then may be identified and isolated. In this manner, the retroviruses function as promoter-traps. Related methods and products including vectors, kits and assays provided.
Abstract: A tissue or cell growth device is described for placement within a well of a cluster dish. The device has a cell or tissue retention element detachably attached to a hanger. The hanger preferably has openings allowing access to the well without removal of the member and is capable of positioning the cell or tissue retention element a preselected distance relative to the hanger. Also described are various embodiments of the device having differently shaped hangers and the combination of the device and a cluster dish.
Type:
Grant
Filed:
October 10, 1990
Date of Patent:
August 18, 1992
Assignee:
Costar Corporation
Inventors:
David Butz, George Lyman, David Root, Gregory Mathus
Abstract: A chandelier having a frame for supporting ornaments with loop shafts attached thereto provides a frame member having a predetermined thickness and a width between an inner edge and an outer edge. An ornament shaft that terminates in a loop has segments that engage a width defining edges of the frame member while the ornament is positioned in a vertical hanging orientation. The ornament further includes an opening defined by the shaft and a portion of the loop that is constructed and arranged so that the frame member may be engaged with and disengaged from the loop only through the opening and only by first rotating the shaft substantially out of the vertical orientation. The loop is further constructed and arranged so that substantially vertical displacement is prevented. The opening may be defined by an end of the loop and the shaft and the frame member may include one or more formations or indentations having a width less than the width of adjacent sections for supporting the loop.
Abstract: The invention provides methods and devices for conducting affinity electrophoresis and for characterizing, identifying, selecting, separating and/or isolating molecules based upon affinity. Acceptor molecules are dispersed in discrete lanes within a gel and test molecules are electrophoresed simultaneously through the lanes. Therapeutics may be screened, heterogeneous mixes may be separated and affinity equilibrium constants determined. Special combs and casting trays for forming gels also are provided, as well as the gels formed thereby. An electrophoresis box also is provided.
Abstract: A photographic image is prepared for facsimile transmission by photocopying the photographic image onto a sheet of paper through a partially transparent screen. The screen may comprise a plurality of spaced dots on a sheet in which one of the sheet and dots are transparent and the other opaque to light transmission whereby the photocopy has reproduced on it the picture and the pattern of the dots with either the dots or the spaces between the dots forming the image of the picture.