Patents Represented by Attorney, Agent or Law Firm Elias J. Lambiris, Esq.
  • Patent number: 6423524
    Abstract: The present invention relates to cellulase preparations consisting essentially of a homogeneous endoglucanase component. The cellulase preparation may be employed in the treatment of cellulose-containing fabrics for harshness reduction, for color clarification, or to provide a localized variation in the color of such fabrics, or in the treatment of paper pulp.
    Type: Grant
    Filed: November 10, 1998
    Date of Patent: July 23, 2002
    Assignee: Novo Nordisk A/S
    Inventors: Fred Hagen, Carsten Mailand Hjort, Sven Hastrup
  • Patent number: 6372465
    Abstract: The present invention relates to variants of a parent vanadium-containing haloperoxidase, which variant has haloperoxidase activity and an altered pH optimum and comprises a mutation in a position corresponding to at least one of the following positions: R490A,L,I,Q,M,E,D; A399G; F397N,Y,E,Q; P395A,S; R360A,L,I,Q,M,E,D; K353Q,M; S402A,T,V,S; D292L; A501S; W350F,Y; V495A,T,V,S; K394 A,L,I,Q,M,E,D; wherein the parent haloperoxidase has the amino acid sequence given in SEQ ID No. 1, or the parent haloperoxidase has an amino acid sequence which is at least 80% homologous to SEQ ID No. 1.
    Type: Grant
    Filed: February 20, 2001
    Date of Patent: April 16, 2002
    Assignee: Novozymes A/S
    Inventors: Allan Svendsen, Louise Jørgensen
  • Patent number: 6365204
    Abstract: Disclosed is a process for preparing a dough or a baked product from the dough which process involves incorporating into the dough an anti-staling amylase and a phospholipase. The bread made by the combined use of an anti-staling amylase and a phospholipase has improved softness, both when eaten on the same day and when stored for several days after baking. There is no significant change in the taste or smell of the baked product.
    Type: Grant
    Filed: April 5, 1999
    Date of Patent: April 2, 2002
    Assignee: Novozymes
    Inventors: Tina Spendler, Lone Nilsson, Claus Crone Fuglsang
  • Patent number: 6346401
    Abstract: The present invention deals with a process for oxidation of a hydroxy group of C1 and/or C2 and/or C3 and/or C4 and/or C5 and/or C6 of a sugar monomer of an oligo- or a polysaccharide comprising contacting, in an aqueous medium, the oligo- or the polysaccharide with a phenol oxidizing enzyme and an enhancing agent, whereby an oligo- or a polysaccharide with altered characteristics compared to the native oligo- or polysaccharide is created.
    Type: Grant
    Filed: May 26, 1999
    Date of Patent: February 12, 2002
    Assignee: Novozymes A/S
    Inventor: Jesper Vallentin Kierulff
  • Patent number: 6306631
    Abstract: The present invention relates to a novel ppGpp synthetase and expression systems for improved production of proteins of interest in Gram-positive bacteria, involving use of the novel ppGpp synthetase.
    Type: Grant
    Filed: November 16, 1999
    Date of Patent: October 23, 2001
    Assignee: Novozymes A/S
    Inventors: Jens Tonne Andersen, Stanislas Dusko Ehrlich
  • Patent number: 6306813
    Abstract: Lipases having improved washing performance for use in detergents to improve the removal of fatty soiling, and having activity throughout a temperature range of 1 to 65° C., including an activity at 1° C. which is at least 20% the activity at 30° C., activity over a pH range of 8-12, and activity at high concentration of anionic surfactant and in the presence of bleach.
    Type: Grant
    Filed: September 11, 1998
    Date of Patent: October 23, 2001
    Assignee: Novozymes A/S
    Inventor: Masahiro Suzuki
  • Patent number: 6303752
    Abstract: The present invention relates to modified polypeptides with reduced respiratory allergenicity having polymeric molecules with a molecular weight from 100 up to 750 Da, coupled covalently to the parent polypeptide having a molecular weight from 5 to 100 kDa. The present invention also relates to industrial compositions comprising modified polypeptide with reduced respiratory allergenicity, skin care products, the use of modified polypeptides for reducing the allergenicity of industrial composition and products and finally a method for reducing the allergenicity of polypeptides.
    Type: Grant
    Filed: June 25, 1998
    Date of Patent: October 16, 2001
    Assignee: Novozymes A/S
    Inventors: Arne Agerlin Olsen, Tine Muxoll Fatum, Heinz Josef Deussen, Erwin Ludo Roggen
  • Patent number: 6298859
    Abstract: A process for preparing tobacco, which process comprises the steps of treating a tobacco material with a phenol oxidising enzyme, such as by extracting tobacco with a solvent to provide an extract and a residue; and treating the extract with a phenol oxidising enzyme such as a laccase. An improved tobacco product having a reduced amount of phenolic compounds. This is an alternative or a supplement to a process in which the phenolic compounds are adsorbed onto the insoluble carrier polyvinylpolypyrrolidone (PVPP). In preferred embodiments, the process includes further steps of removing the oxidised phenolic compound, adding adsorbents such as bentonite; removing and/or inactivating the enzyme; and concentrating the extract. Preferred phenol oxidising enzymes are peroxidases and laccases. The thus treated extract is advantageously re-combined with the tobacco residue and further processed to provide a tobacco article for smoking.
    Type: Grant
    Filed: July 7, 1999
    Date of Patent: October 9, 2001
    Assignee: Novozymes A/S
    Inventors: Jesper Vallentin Kierulff, Ole Bill Jørgensen, Tomas Tage Hansen, Anthony James Knox, Yves De Grandpré
  • Patent number: 6300116
    Abstract: The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved autoproteolytic stability in comparison to their wild type parent enzymes.
    Type: Grant
    Filed: November 4, 1997
    Date of Patent: October 9, 2001
    Assignee: Novozymes A/S
    Inventors: Claus von der Osten, Torben Halkier, Carsten Andersen, Peter Bauditz, Peter Kamp Hansen
  • Patent number: 6296672
    Abstract: The present invention relates to methods of dyeing a material which involve contacting the material with a dyeing system which comprises: (a) a mixture of (i) an aromatic diamine and (ii) one or more of a naphthol and an aminonaphthalene and (b) an oxidation system comprising (i) a hydrogen peroxide source and an enzyme exhibiting peroxidase activity or (ii) an enzyme exibiting oxidase activity on one or more of the compounds of mixture (a). The material may be a fabric, yarn, fiber, garment or film made of fur, hide, leather, silk or wool, or made of cationic polysaccharide, cotton, diacetate, flax, linen, lyocel, polyacrylic, synthetic polyamide, polyester, ramie, rayon, triacetate, or viscose.
    Type: Grant
    Filed: December 14, 1999
    Date of Patent: October 2, 2001
    Assignees: Novozymes A/S Patents, Novozymes North America
    Inventors: Martin Barfoed, Ole Kirk, Sonja Salmon
  • Patent number: 6287826
    Abstract: The present invention relates to a process for the preparation of a glucose syrup wherein starch is treated with a Termamyl-like &agr;-amylase comprising a substitution in Val54 shown in SEQ ID NO: 2 or in the corresponding position in another Termamyl-like &agr;-amylase. The invention also relates to a glucose syrup obtainable by the process of the invention and the use thereof as ingredient in food products. An object of the invention is also to provide for the use of a Termamyl-like &agr;-amylase with a substitution in position Val54 using SEQ ID NO: 2 as the backbone or a corresponding position in another Termamyl-like &agr;-amylase for preparing glucose syrup.
    Type: Grant
    Filed: March 8, 1999
    Date of Patent: September 11, 2001
    Assignee: Novo Nordisk A/S
    Inventors: Barrie Edmund Norman, Hanne Vang Hendriksen
  • Patent number: 6270813
    Abstract: A process for preparing a dough or a baked product comprises adding an amylase to the dough in an amount which is effective to retard the staling of the bread. The amylase is an exo-amylase which hydrolyzes starch to form mainly mal-totriose.
    Type: Grant
    Filed: March 28, 2000
    Date of Patent: August 7, 2001
    Assignee: Novozymes A/S
    Inventors: Jack Beck Nielsen, Thomas Schäfer
  • Patent number: 6268197
    Abstract: A xyloglucanase having a relative xyloglucanase activity of at least 50% at pH 7 and either no or an insignificant cellulolytic activity is obtainable e.g. from a strain of Bacillus. A xyloglucanase comprising an amino acid sequence as shown in positions 30-261 of SEQ ID NO:2 or homologues may be derived from eg Bacillus licheniformis, ATCC 14580, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 88 to nucleotide 783; and a xyloglucanase comprising an amino acid sequence as shown in positions 1-537 of SEQ ID NO:4 or homologues may be derived from eg B. agaradhaerens, NCIMB 40482, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:3 from nucleotide 1 to nucleotide 1611. The xyloglucanases are useful e.g. in cleaning compositions and for treatment of cellulosic fibres.
    Type: Grant
    Filed: July 7, 1998
    Date of Patent: July 31, 2001
    Assignee: Novozymes A/S
    Inventors: Martin Schulein, Helle Outtrup, Per Lina Jorgensen, Mads Eskelund Bjornvad
  • Patent number: 6268343
    Abstract: The present invention relates to GLP-1 derivatives having a lipophilic substituent, pharmaceutical compositions comprising same, and methods of making an using same. The GLP-1 derivatives of the present invention have a protracted profile of action.
    Type: Grant
    Filed: February 26, 1999
    Date of Patent: July 31, 2001
    Assignee: Novo Nordisk A/S
    Inventors: Liselotte Bjerre Knudsen, Per Olaf Huusfeldt, Per Franklin Nielsen, Niels C. Kaarsholm, Helle Birk Olsen, Søren Erik Bjørn, Freddy Zimmerdahl Pedersen, Kjeld Madsen
  • Patent number: 6261828
    Abstract: A one-step process for combined desizing and “stone-washing” of dyed denim, wherein the denim is treated with an amylolytic enzyme in combination with a first abrading monocomponent endoglucanase and a second streak-reducing monocomponent endoglucanase.
    Type: Grant
    Filed: April 29, 1998
    Date of Patent: July 17, 2001
    Assignee: Novo Nordisk A/S
    Inventor: Henrik Lund
  • Patent number: 6255084
    Abstract: The invention relates to an isolated thermostable glucoamylase derived from Talaromyces emersonii suitable for starch conversion processes.
    Type: Grant
    Filed: November 24, 1998
    Date of Patent: July 3, 2001
    Assignee: Novozymes A/S
    Inventors: Bjarne Rønfeldt Nielsen, Ruby Ilum Nielsen, Jan Lehmbeck
  • Patent number: 6242237
    Abstract: The present invention relates to an enzyme with galactanase activity, a DNA construct encoding the enzyme with galactanase activity, a method of producing the enzyme, an enzyme composition comprising the enzyme with galactanase activity, and the use of the galactanase enzyme and enzyme composition for a number of industrial applications.
    Type: Grant
    Filed: August 21, 1998
    Date of Patent: June 5, 2001
    Assignee: Novo Nordisk A/S
    Inventors: Lene Venke Kofod, Markus Sakari Kauppinen, Lene Nonboe Andersen, Ib Groth Clausen, Anette Müllertz
  • Patent number: 6232101
    Abstract: A method for causing gelling or increase of viscosity of an aqueous medium containing a gellable polymeric material having substituents with phenolic hydroxy groups comprises adding an oxidase, particularly a laccase, to the aqueous medium.
    Type: Grant
    Filed: October 28, 1996
    Date of Patent: May 15, 2001
    Assignee: Novozymes A/S Patents
    Inventors: Gitte Budolfsen, Hans Peter Heldt-Hansen
  • Patent number: 6231621
    Abstract: The invention relates to substrates which develops color on oxidation of general formula (I). The invention also relates to the use of said substrates for dyeing, e.g. keratinous fibers, in particular hair, dyeing textiles, a dyeing composition and a method for dyeing keratinous fibers.
    Type: Grant
    Filed: March 18, 1999
    Date of Patent: May 15, 2001
    Assignee: Novozymes A/S
    Inventors: Niels Henrik Sørensen, Ole Kirk, Rikke Lolck, Torben Desler
  • Patent number: 6228630
    Abstract: An enzyme preparation comprising (a) first enzyme with xylanase activity and (b) a second enzyme having cellulotytic, xylanolytic or pectinolytic activity and are supported by the specification.
    Type: Grant
    Filed: June 22, 2000
    Date of Patent: May 8, 2001
    Assignee: Novo Nordisk A/S
    Inventors: Lene Venke Kofod, MArkus Sakari Kauppinen, Stephan Christgau, Hans Peter Heldt-Hansen, Henrik Dalbøge, Lene Nonboe Andersen, Joan Qi Si, Tina Sejersgåard Jacobsen, Niels Munk, Anette Müllertz